{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,1]],"date-time":"2026-09-01T09:18:12Z","timestamp":1788254292398,"version":"build-2803163510"},"reference-count":33,"publisher":"American Chemical Society (ACS)","issue":"33","license":[{"start":{"date-parts":[[2026,8,19]],"date-time":"2026-08-19T00:00:00Z","timestamp":1787097600000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-029"},{"start":{"date-parts":[[2026,8,19]],"date-time":"2026-08-19T00:00:00Z","timestamp":1787097600000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-037"},{"start":{"date-parts":[[2026,8,19]],"date-time":"2026-08-19T00:00:00Z","timestamp":1787097600000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-045"}],"funder":[{"DOI":"10.13039\/501100018537","name":"National Science and Technology Major Project","doi-asserted-by":"publisher","award":["2026ZD1806901"],"award-info":[{"award-number":["2026ZD1806901"]}],"id":[{"id":"10.13039\/501100018537","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2026,8,26]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>The phytopathogen Fusarium oxysporum threatens global food security, necessitating sustainable biocontrol alternatives to chemical fungicides. Here, we employed an OSMAC-driven dual-layer fast screening (DLFS) approach to identify Streptomyces sp. OUC-HL1638 as a potent antagonist. Through integrated NMR-based lipopeptide signature mapping and LC-MS-assisted NRPS gene mining, we efficiently targeted and isolated two stendomycins (1 and 2). These compounds exhibited potent in vitro antifungal activity (MIC = 1 \u03bcM), outperforming hymexazol by 1000-fold, and effectively controlled F. oxysporum infection in radish seedlings and postharvest cherry tomatoes without phytotoxicity. The producing strain intrinsically balances stendomycin production with low plant toxicity and sustains production under high-salinity and alkaline conditions, indicating potential for saline-alkaline agriculture. This work establishes an efficient pipeline for green fungicide discovery and highlights the promise of stendomycins and strain OUC-HL1638 as sustainable biocontrol agents for crop protection.<\/jats:p>","DOI":"10.1021\/acs.jafc.6c00886","type":"journal-article","created":{"date-parts":[[2026,8,19]],"date-time":"2026-08-19T05:02:07Z","timestamp":1787115727000},"page":"26209-26220","source":"Crossref","is-referenced-by-count":0,"title":["Combating\n                    <i>Fusarium<\/i>\n                    Wilt: A Metabologenomics\nApproach Identifies Stendomycins as Promising Sustainable Biocontrol\nAgents"],"prefix":"10.1021","volume":"74","author":[{"given":"Jingyi","family":"Lyu","sequence":"first","affiliation":[{"name":"Ocean University of China , , ,","place":["Qingdao, China, 266003"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hu","family":"Chen","sequence":"additional","affiliation":[{"name":"Ocean University of China , , ,","place":["Qingdao, China, 266003"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Junjie","family":"Liu","sequence":"additional","affiliation":[{"name":"Ocean University of China , , ,","place":["Qingdao, China, 266003"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4639-7851","authenticated-orcid":true,"given":"Ming","family":"Lu","sequence":"additional","affiliation":[{"name":"Qingdao Institute of Bioenergy and Bioprocess Technology, Chinese Academy of Sciences , , ,","place":["Qingdao, China, 266101"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9186-1804","authenticated-orcid":true,"given":"Huayue","family":"Li","sequence":"additional","affiliation":[{"name":"Ocean University of China , , ,","place":["Qingdao, China, 266003"]},{"name":"Qingdao National Laboratory for Marine Science and Technology , , ,","place":["Qingdao, China, 266003"]}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"316","published-online":{"date-parts":[[2026,8,19]]},"reference":[{"issue":"4","key":"2026090104470382600_cit1","doi-asserted-by":"publisher","first-page":"414","DOI":"10.1111\/j.1364-3703.2011.00783.x","article-title":"The Top\n10 fungal\npathogens in molecular plant pathology","volume":"13","author":"Dean","year":"2012","journal-title":"Mol.\nPlant Pathol."},{"issue":"4","key":"2026090104470382600_cit2","doi-asserted-by":"publisher","first-page":"512","DOI":"10.1094\/PHYTO-08-18-0320-RVW","article-title":"Current status of Fusarium oxysporumformae speciales and races","volume":"109","author":"Edel-Hermann","year":"2019","journal-title":"Phytopathology"},{"issue":"7","key":"2026090104470382600_cit3","doi-asserted-by":"publisher","first-page":"717","DOI":"10.1016\/S2666-5247(24)00042-9","article-title":"The WHO fungal priority pathogens\nlist: a crucial reappraisal to review the prioritisation","volume":"5","author":"Casalini","year":"2024","journal-title":"Lancet Microbe"},{"issue":"3","key":"2026090104470382600_cit4","doi-asserted-by":"publisher","first-page":"258","DOI":"10.1016\/j.jiac.2023.10.011","article-title":"Fusarium oxysporum disseminated\ninfection in a teenage patient with a relapse of acute lymphoblastic\nleukemia-case report and review of the literature","volume":"30","author":"Samborska","year":"2024","journal-title":"J. Infect. Chemother."},{"issue":"1","key":"2026090104470382600_cit5","doi-asserted-by":"publisher","first-page":"13","DOI":"10.1007\/s40572-020-00266-5","article-title":"Synthetic pesticides\nand health in vulnerable populations: agricultural workers","volume":"7","author":"Curl","year":"2020","journal-title":"Curr. Environ. Health Rep."},{"key":"2026090104470382600_cit6","doi-asserted-by":"publisher","DOI":"10.1016\/j.scitotenv.2019.134612","article-title":"Pesticides in the urban\nenvironment: a potential threat\nthat knocks at the door","volume":"711","author":"Meftaul","year":"2020","journal-title":"Sci. Total Environ."},{"issue":"4","key":"2026090104470382600_cit7","doi-asserted-by":"publisher","first-page":"707","DOI":"10.1094\/PHYTO-10-22-0370-KD","article-title":"Fungicide resistance: progress in\nunderstanding mechanism, monitoring,\nand management","volume":"113","author":"Yin","year":"2023","journal-title":"Phytopathology"},{"key":"2026090104470382600_cit8","doi-asserted-by":"publisher","DOI":"10.3389\/fmicb.2023.1040901","article-title":"Biopesticides as a promising alternative to synthetic\npesticides: a case for microbial pesticides, phytopesticides, and\nnanobiopesticides","volume":"14","author":"Ayilara","year":"2023","journal-title":"Front. 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Microbiol."},{"issue":"42","key":"2026090104470382600_cit13","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.2304668120","article-title":"Identification\nof the lipodepsipeptide selethramide encoded in a giant nonribosomal\npeptide synthetase from a Burkholderia bacterium","volume":"120","author":"Romanowski","year":"2023","journal-title":"P. Natl. Acad. Sci. U.S.A."},{"key":"2026090104470382600_cit14","doi-asserted-by":"publisher","DOI":"10.1016\/j.micres.2023.127518","article-title":"Structural diversity\nand applications\nof lipopeptide biosurfactants as biocontrol agents against phytopathogens:\na review","volume":"278","author":"Sani","year":"2024","journal-title":"Microbiol. Res."},{"key":"2026090104470382600_cit15","doi-asserted-by":"publisher","first-page":"471","DOI":"10.1016\/j.tifs.2021.04.019","article-title":"Antimicrobial\npeptides and their application in food packaging","volume":"112","author":"Liu","year":"2021","journal-title":"Trends Food Sci. 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Chem."},{"issue":"11","key":"2026090104470382600_cit28","doi-asserted-by":"publisher","first-page":"794","DOI":"10.1038\/nchembio.684","article-title":"A mass spectrometry-guided genome\nmining approach for natural product peptidogenomics","volume":"7","author":"Kersten","year":"2011","journal-title":"Nat. Chem. Biol."},{"issue":"46","key":"2026090104470382600_cit29","doi-asserted-by":"publisher","first-page":"19425","DOI":"10.1021\/jacs.1c08177","article-title":"Enzymatic\nC\u03b2-H functionalization of\nL-Arg and L-Leu in nonribosomally derived peptidyl natural products:\na tale of two oxidoreductases","volume":"143","author":"Cui","year":"2021","journal-title":"J. Am. Chem.\nSoc."},{"key":"2026090104470382600_cit30","doi-asserted-by":"publisher","first-page":"1239","DOI":"10.1038\/nchembio.2493","article-title":"Stendomycin\nselectively inhibits TIM23-dependent mitochondrial protein import","volume":"13","author":"Filipuzzi","year":"2017","journal-title":"Nat. Chem. Biol."},{"key":"2026090104470382600_cit31","doi-asserted-by":"publisher","DOI":"10.3389\/fpls.2014.00082","article-title":"Evidence for interactions\nbetween the mitochondrial import apparatus and respiratory chain complexes\nvia Tim21-like proteins in Arabidopsis","volume":"5","author":"Murcha","year":"2014","journal-title":"Front.\nPlant Sci."},{"key":"2026090104470382600_cit32","doi-asserted-by":"publisher","first-page":"1737","DOI":"10.1104\/pp.102.016808","article-title":"Identification, expression,\nand import of components 17 and 23 of the inner mitochondrial membrane\ntranslocase from Arabidopsis","volume":"131","author":"Murcha","year":"2003","journal-title":"Plant Physiol."},{"issue":"2","key":"2026090104470382600_cit33","doi-asserted-by":"publisher","first-page":"304","DOI":"10.1016\/j.bbamcr.2012.02.015","article-title":"Unique components\nof the plant mitochondrial\nprotein import apparatus","volume":"1833","author":"Duncan","year":"2013","journal-title":"Biochim. Biophys.\nActa, Mol. Cell Res."}],"container-title":["Journal of Agricultural\nand Food Chemistry"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/pubs.acs.org\/doi\/pdf\/10.1021\/acs.jafc.6c00886","content-type":"application\/pdf","content-version":"vor","intended-application":"unspecified"},{"URL":"https:\/\/pubs.acs.org\/jafcau\/article-pdf\/74\/33\/26209\/66856070\/acs.jafc.6c00886.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"syndication"},{"URL":"https:\/\/pubs.acs.org\/jafcau\/article-pdf\/74\/33\/26209\/66856070\/acs.jafc.6c00886.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,9,1]],"date-time":"2026-09-01T08:47:23Z","timestamp":1788252443000},"score":1,"resource":{"primary":{"URL":"https:\/\/pubs.acs.org\/jafcau\/article\/74\/33\/26209\/5275834\/Combating-Fusarium-Wilt-A-Metabologenomics"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,8,19]]},"references-count":33,"journal-issue":{"issue":"33","published-online":{"date-parts":[[2026,8,19]]},"published-print":{"date-parts":[[2026,8,26]]}},"URL":"https:\/\/doi.org\/10.1021\/acs.jafc.6c00886","relation":{},"ISSN":["0021-8561","1520-5118"],"issn-type":[{"value":"0021-8561","type":"print"},{"value":"1520-5118","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026,8,19]]}}}