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The results of bioassays showed that compound Z-2 possessed potent activity against Rhizoctonia solani and Botrytis cinereaPers with EC50 values of 7.03 and 13.5 mg\/L, respectively. Compound Z-3 (EC50 = 6.81 mg\/L) exhibited significant activity against Sclerotinia sclerotiorum. In vivo antifungal bioassays suggested that compounds Z-2 and Z-3 could serve as novel agricultural antifungals. Cell membrane permeability and PI staining experiments demonstrated that compounds Z-2 and Z-3 impaired hyphal membrane integrity, leading to cytoplasmic leakage and suppression of hyphal growth. SEM and TEM revealed that compound Z-2 induced distinct morphological alterations in the hyphae, thereby suppressing hyphal growth. Molecular docking and DFT calculations confirmed that compounds Z-2 and Z-3 share highly similar binding modes. Plant safety tests verified their low phytotoxicity. 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