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This study demonstrates that sulfonyl-containing sweeteners enhance GOD activity and catalytic efficiency. Rapid kinetic analysis of allosteric transitions and redox reactions revealed that glucose recognition follows the induced-fit model: glucose induces conformational changes in GOD, enabling the redox reaction. The sweeteners increase GOD\u2019s conformational flexibility and substrate affinity while reducing the activation energy for allostery. This facilitates the transition between active and inactive states and accelerates the catalytic function of flavin adenine dinucleotide toward the substrate. These findings provide deeper insights into GOD catalysis and regulation, opening new avenues for its diverse applications.<\/jats:p>","DOI":"10.1021\/acs.jafc.6c01746","type":"journal-article","created":{"date-parts":[[2026,9,3]],"date-time":"2026-09-03T19:57:44Z","timestamp":1788465464000},"page":"28641-28653","source":"Crossref","is-referenced-by-count":1,"title":["Mechanistic Insight\ninto the Enhancement of Glucose\nOxidase Activity by Sulfonyl-Containing Sweeteners"],"prefix":"10.1021","volume":"74","author":[{"given":"Yu","family":"Sun","sequence":"first","affiliation":[{"name":"Zhejiang Gongshang University , , No. 18 Xuezheng Street , ,","place":["Hangzhou, China, 310018"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Pan-Pan","family":"Chen","sequence":"additional","affiliation":[{"name":"Zhejiang Gongshang University , , No. 18 Xuezheng Street , ,","place":["Hangzhou, China, 310018"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Biao","family":"Wang","sequence":"additional","affiliation":[{"name":"Zhejiang Gongshang University , , No. 18 Xuezheng Street , ,","place":["Hangzhou, China, 310018"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yu-Long","family":"Sun","sequence":"additional","affiliation":[{"name":"Zhejiang Gongshang University , , No. 18 Xuezheng Street , ,","place":["Hangzhou, China, 310018"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Li","family":"Zhang","sequence":"additional","affiliation":[{"name":"Acon Biotech (Hangzhou) Co., Ltd. , No. 210 Zhenzhong Road, Aicheng Industrial Park , , ,","place":["Hangzhou, Zhejiang, China, 310030"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1230-4859","authenticated-orcid":true,"given":"Zhong-Xiu","family":"Chen","sequence":"additional","affiliation":[{"name":"Zhejiang Gongshang University , , No. 18 Xuezheng Street , ,","place":["Hangzhou, China, 310018"]}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"316","published-online":{"date-parts":[[2026,9,3]]},"reference":[{"key":"2026091609095327300_cit1","doi-asserted-by":"publisher","first-page":"472","DOI":"10.3390\/biom12030472","article-title":"Glucose Oxidase, an Enzyme \"Ferrari\":\nIts Structure, Function, Production and Properties in the Light of\nVarious Industrial and Biotechnological Applications","volume":"12","author":"Bauer","year":"2022","journal-title":"Biomolecules"},{"issue":"1","key":"2026091609095327300_cit2","doi-asserted-by":"publisher","first-page":"82","DOI":"10.1177\/1932296818791538","article-title":"In Vitro Sugar Interference\nTesting With Amperometric\nGlucose Oxidase Sensors","volume":"13","author":"Boehm","year":"2019","journal-title":"J. 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