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Most compounds showed high in vitro activity against Rhizoctonia solani, with EC50 values below 0.01 mg\/L. Compound V-17b (4-OPh) was the most potent (EC50 = 0.0029 mg\/L), surpassing boscalid and fluxapyroxad by over 10-fold. Several compounds retained &amp;gt;50% inhibition in vivo at 200 mg\/L against Rhizoctonia solani. Compound V-6b exhibited 100% efficacy against Phakopsora pachyrhizi at 1 mg\/L, and V-9b showed 77.8% and 96.3% efficacy against Puccinia glumarum and Puccinia sorghi at 10 mg\/L, respectively. Enzyme assays revealed that V-9b inhibited porcine SDH (IC50 = 1.87 \u03bcM) more effectively than fluxapyroxad (IC50 = 8.13 \u03bcM), with the trans isomer superior to the cis. Molecular docking indicated that the trans configuration enables additional \u03c0\u2013\u03c0 stacking with Trp_B173. These results demonstrate that the monofluoroalkene scaffold, combined with appropriate substitution patterns and configuration, can generate potent SDHIs with promising fungicidal profiles.<\/jats:p>","DOI":"10.1021\/acs.jafc.6c02371","type":"journal-article","created":{"date-parts":[[2026,9,29]],"date-time":"2026-09-29T20:02:01Z","timestamp":1790712121000},"page":"31329-31342","source":"Crossref","is-referenced-by-count":0,"title":["Discovery of a\n                    <i>trans<\/i>\n                    -Configured Monofluoroalkene\nas a Potent Succinate Dehydrogenase Inhibitor against\n                    <i>Phakopsora pachyrhizi<\/i>"],"prefix":"10.1021","volume":"74","author":[{"given":"Letian","family":"Zhang","sequence":"first","affiliation":[{"name":"East China University of Science and Technology , , ,","place":["Shanghai, China, 200237"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yuchen","family":"Li","sequence":"additional","affiliation":[{"name":"East China University of Science and Technology , , ,","place":["Shanghai, China, 200237"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Wende","family":"Wu","sequence":"additional","affiliation":[{"name":"East China University of Science and Technology , , ,","place":["Shanghai, China, 200237"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6665-5040","authenticated-orcid":true,"given":"Zhong","family":"Li","sequence":"additional","affiliation":[{"name":"East China University of Science and Technology , , ,","place":["Shanghai, China, 200237"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9679-3153","authenticated-orcid":true,"given":"Xiaoyong","family":"Xu","sequence":"additional","affiliation":[{"name":"East China University of Science and Technology , , ,","place":["Shanghai, China, 200237"]}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"316","published-online":{"date-parts":[[2026,9,29]]},"reference":[{"key":"2026101408085532200_cit1","doi-asserted-by":"publisher","first-page":"917","DOI":"10.1016\/j.ejmech.2018.08.040","article-title":"A review on diverse heterocyclic\ncompounds as the privileged scaffolds in antimalarial drug discovery","volume":"158","author":"Kalaria","year":"2018","journal-title":"Eur. 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