{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,9]],"date-time":"2026-09-09T03:43:31Z","timestamp":1788925411559,"version":"build-2803163510"},"reference-count":45,"publisher":"American Chemical Society (ACS)","issue":"20","license":[{"start":{"date-parts":[[2026,5,12]],"date-time":"2026-05-12T00:00:00Z","timestamp":1778544000000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-029"},{"start":{"date-parts":[[2026,5,12]],"date-time":"2026-05-12T00:00:00Z","timestamp":1778544000000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-037"},{"start":{"date-parts":[[2026,5,12]],"date-time":"2026-05-12T00:00:00Z","timestamp":1778544000000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-045"}],"funder":[{"DOI":"10.13039\/501100012166","name":"National Key Research and Development Program of China","doi-asserted-by":"publisher","award":["2021YFC2103800"],"award-info":[{"award-number":["2021YFC2103800"]}],"id":[{"id":"10.13039\/501100012166","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100004608","name":"Natural Science Foundation of Jiangsu Province","doi-asserted-by":"publisher","award":["BK20253004"],"award-info":[{"award-number":["BK20253004"]}],"id":[{"id":"10.13039\/501100004608","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["82204192"],"award-info":[{"award-number":["82204192"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["J. Agric. Food Chem."],"published-print":{"date-parts":[[2026,5,27]]},"DOI":"10.1021\/acs.jafc.6c02908","type":"journal-article","created":{"date-parts":[[2026,5,12]],"date-time":"2026-05-12T19:06:28Z","timestamp":1778612788000},"page":"15727-15736","source":"Crossref","is-referenced-by-count":1,"title":["Computational-Aided Mutagenesis of Lipase CRL1 with Promoted Stability and Activity for Biosynthesis of Vitamin E Succinate"],"prefix":"10.1021","volume":"74","author":[{"given":"Wenyu","family":"Shao","sequence":"first","affiliation":[{"name":"College of Biotechnology and Pharmaceutical Engineering, Nanjing Tech University, 30 Puzhunan Road, Jiangbei New Area, Nanjing 211800, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yu","family":"Zhang","sequence":"additional","affiliation":[{"name":"College of Biotechnology and Pharmaceutical Engineering, Nanjing Tech University, 30 Puzhunan Road, Jiangbei New Area, Nanjing 211800, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jialing","family":"Wang","sequence":"additional","affiliation":[{"name":"College of Biotechnology and Pharmaceutical Engineering, Nanjing Tech University, 30 Puzhunan Road, Jiangbei New Area, Nanjing 211800, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6999-1576","authenticated-orcid":true,"given":"BingBing","family":"Gao","sequence":"additional","affiliation":[{"name":"School of Pharmaceutical Sciences, Nanjing Tech University, 30 Puzhunan Road, Jiangbei New Area, Nanjing 211800, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8197-6459","authenticated-orcid":true,"given":"Jianlin","family":"Chu","sequence":"additional","affiliation":[{"name":"School of Pharmaceutical Sciences, Nanjing Tech University, 30 Puzhunan Road, Jiangbei New Area, Nanjing 211800, China"},{"name":"Jiangsu Provincial University Key Laboratory of Green Biomanufacturing for Pharmaceuticals, School of Pharmaceutical Sciences","place":["Nanjing, China"]},{"name":"Nanjing Tech University","place":["Nanjing, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zhen","family":"Gao","sequence":"additional","affiliation":[{"name":"College of Biotechnology and Pharmaceutical Engineering, Nanjing Tech University, 30 Puzhunan Road, Jiangbei New Area, Nanjing 211800, China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"316","published-online":{"date-parts":[[2026,5,12]]},"reference":[{"key":"ref1\/cit1","doi-asserted-by":"publisher","DOI":"10.1179\/135100001101536247"},{"key":"ref2\/cit2","doi-asserted-by":"publisher","DOI":"10.3390\/catal11060739"},{"key":"ref3\/cit3","doi-asserted-by":"publisher","DOI":"10.3390\/molecules30061241"},{"key":"ref4\/cit4","doi-asserted-by":"publisher","DOI":"10.1016\/j.bbagen.2023.130485"},{"key":"ref5\/cit5","doi-asserted-by":"publisher","DOI":"10.1111\/j.1742-7843.2004.950305.x"},{"key":"ref6\/cit6","doi-asserted-by":"publisher","DOI":"10.1016\/j.ijpharm.2021.120457"},{"key":"ref7\/cit7","doi-asserted-by":"publisher","DOI":"10.3389\/fnut.2023.1281879"},{"key":"ref8\/cit8","doi-asserted-by":"publisher","DOI":"10.1134\/S0006297916080034"},{"key":"ref9\/cit9","doi-asserted-by":"publisher","DOI":"10.1016\/j.biotechadv.2022.108002"},{"key":"ref10\/cit10","doi-asserted-by":"publisher","DOI":"10.1016\/j.pbiomolbio.2017.07.014"},{"key":"ref11\/cit11","doi-asserted-by":"publisher","DOI":"10.1002\/anie.202217878"},{"key":"ref12\/cit12","doi-asserted-by":"publisher","DOI":"10.1016\/j.biotechadv.2013.08.006"},{"key":"ref13\/cit13","doi-asserted-by":"publisher","DOI":"10.1039\/C5GC02557E"},{"key":"ref14\/cit14","doi-asserted-by":"publisher","DOI":"10.6023\/A24100326"},{"key":"ref15\/cit15","doi-asserted-by":"publisher","DOI":"10.1016\/S1004-9541(09)60189-0"},{"key":"ref16\/cit16","doi-asserted-by":"publisher","DOI":"10.1007\/s40242-013-2486-z"},{"key":"ref17\/cit17","doi-asserted-by":"publisher","DOI":"10.1023\/B:BILE.0000023020.12576.1e"},{"key":"ref18\/cit18","doi-asserted-by":"publisher","DOI":"10.1016\/j.bbrc.2021.02.003"},{"key":"ref19\/cit19","doi-asserted-by":"publisher","DOI":"10.1002\/aocs.12847"},{"key":"ref20\/cit20","doi-asserted-by":"publisher","DOI":"10.1002\/jsfa.10947"},{"key":"ref21\/cit21","doi-asserted-by":"publisher","DOI":"10.1021\/acs.langmuir.5c03561"},{"key":"ref22\/cit22","doi-asserted-by":"publisher","DOI":"10.1016\/j.biotechadv.2025.108658"},{"key":"ref23\/cit23","doi-asserted-by":"publisher","DOI":"10.1007\/s00253-021-11520-7"},{"key":"ref24\/cit24","doi-asserted-by":"publisher","DOI":"10.1016\/j.enzmictec.2015.09.003"},{"key":"ref25\/cit25","doi-asserted-by":"publisher","DOI":"10.1016\/bs.mie.2020.07.010"},{"key":"ref26\/cit26","doi-asserted-by":"publisher","DOI":"10.1039\/D2CY00882C"},{"key":"ref27\/cit27","doi-asserted-by":"publisher","DOI":"10.1016\/S1872-2067(25)64767-X"},{"key":"ref28\/cit28","doi-asserted-by":"publisher","DOI":"10.1016\/j.ijbiomac.2025.142646"},{"key":"ref29\/cit29","doi-asserted-by":"publisher","DOI":"10.1016\/j.mcat.2021.111937"},{"key":"ref30\/cit30","doi-asserted-by":"publisher","DOI":"10.1016\/j.bbrc.2025.151991"},{"key":"ref31\/cit31","doi-asserted-by":"publisher","DOI":"10.1016\/j.biotechadv.2024.108376"},{"key":"ref32\/cit32","doi-asserted-by":"publisher","DOI":"10.1021\/acs.chemrev.8b00290"},{"key":"ref33\/cit33","doi-asserted-by":"publisher","DOI":"10.1021\/acscatal.3c00503"},{"key":"ref34\/cit34","doi-asserted-by":"publisher","DOI":"10.1021\/cs500325p"},{"key":"ref35\/cit35","doi-asserted-by":"publisher","DOI":"10.1016\/j.abb.2017.07.003"},{"key":"ref36\/cit36","doi-asserted-by":"publisher","DOI":"10.1016\/j.xinn.2024.100750"},{"key":"ref37\/cit37","doi-asserted-by":"publisher","DOI":"10.1038\/srep33797"},{"key":"ref38\/cit38","doi-asserted-by":"publisher","DOI":"10.1021\/acscatal.5c03699"},{"key":"ref39\/cit39","doi-asserted-by":"publisher","DOI":"10.1007\/s12033-018-0062-y"},{"key":"ref40\/cit40","doi-asserted-by":"publisher","DOI":"10.1016\/j.ijbiomac.2025.142543"},{"key":"ref41\/cit41","doi-asserted-by":"publisher","DOI":"10.1007\/s00253-019-09790-3"},{"key":"ref42\/cit42","doi-asserted-by":"publisher","DOI":"10.1007\/s11274-023-03518-2"},{"key":"ref43\/cit43","doi-asserted-by":"publisher","DOI":"10.1039\/C7RA11679A"},{"key":"ref44\/cit44","doi-asserted-by":"publisher","DOI":"10.1016\/j.ijbiomac.2023.125998"},{"key":"ref45\/cit45","doi-asserted-by":"publisher","DOI":"10.1016\/j.biotechadv.2023.108094"}],"container-title":["Journal of Agricultural and Food Chemistry"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/pubs.acs.org\/doi\/pdf\/10.1021\/acs.jafc.6c02908","content-type":"application\/pdf","content-version":"vor","intended-application":"unspecified"},{"URL":"https:\/\/pubs.acs.org\/doi\/pdf\/10.1021\/acs.jafc.6c02908","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,5,27]],"date-time":"2026-05-27T08:11:50Z","timestamp":1779869510000},"score":1,"resource":{"primary":{"URL":"https:\/\/pubs.acs.org\/doi\/10.1021\/acs.jafc.6c02908"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,5,12]]},"references-count":45,"journal-issue":{"issue":"20","published-print":{"date-parts":[[2026,5,27]]}},"alternative-id":["10.1021\/acs.jafc.6c02908"],"URL":"https:\/\/doi.org\/10.1021\/acs.jafc.6c02908","relation":{},"ISSN":["0021-8561","1520-5118"],"issn-type":[{"value":"0021-8561","type":"print"},{"value":"1520-5118","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026,5,12]]}}}