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To elucidate the underlying regulatory mechanisms, transcriptomic and metabolomic analyses were conducted on pink, gray, and white flower germplasms. Anthocyanin content was higher in pigmented flowers (162.29 \u03bcg\/g) than in white flowers (36.28 \u03bcg\/g). Compared to white flowers, gray flowers showed upregulation of four key metabolites related to petunidin, cyanidin, and delphinidin. Pink flowers encompassed 15 derivatives derived from cyanidin, delphinidin, peonidin, petunidin, and malvidin. Across the three flower colors, 70 structural genes involved in anthocyanin biosynthesis were differentially expressed. Integrated omics and weighted gene coexpression network analysis (WGCNA) identified 15 candidate regulators: ten structural genes (VfCHI4, four CHSs, four UFGTs, VfGST18) and five transcription factors (two MYBs, two bHLHs, one WRKY), all of which showed significant positive correlations with anthocyanin components. These results provide a valuable reference for understanding the molecular mechanisms underlying flower color variation.<\/jats:p>","DOI":"10.1021\/acs.jafc.6c03598","type":"journal-article","created":{"date-parts":[[2026,9,21]],"date-time":"2026-09-21T12:15:04Z","timestamp":1789992904000},"page":"29932-29944","source":"Crossref","is-referenced-by-count":0,"title":["Integrated Transcriptome and Metabolome Analysis Reveals\nthe Molecular Basis Underlying Anthocyanin Biosynthesis Associated\nwith Different Flower Colors in\n                    <i>Vicia faba<\/i>\n                    L."],"prefix":"10.1021","volume":"74","author":[{"given":"Changcai","family":"Teng","sequence":"first","affiliation":[{"name":"Qinghai University , , ,","place":["Xining, Qinghai, China, 810016"]},{"name":"Qinghai Academy of Agricultural and Forestry Sciences , , ,","place":["Xining, Qinghai, China, 810016"]},{"name":"Laboratory of Research and Utilization of Crop Germplasm Resources on the Qinghai\u2212Tibet Plateau , , ,","place":["Xining, Qinghai, China, 810016"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0001-0730-4336","authenticated-orcid":true,"given":"Xianli","family":"Zhou","sequence":"additional","affiliation":[{"name":"Qinghai University , , ,","place":["Xining, Qinghai, China, 810016"]},{"name":"Qinghai Academy of Agricultural and Forestry Sciences , , ,","place":["Xining, Qinghai, China, 810016"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hongyan","family":"Zhang","sequence":"additional","affiliation":[{"name":"Qinghai University , , ,","place":["Xining, Qinghai, China, 810016"]},{"name":"Qinghai Academy of Agricultural and Forestry Sciences , , ,","place":["Xining, Qinghai, China, 810016"]},{"name":"Laboratory of Research and Utilization of Crop Germplasm Resources on the Qinghai\u2212Tibet Plateau , , ,","place":["Xining, Qinghai, China, 810016"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Wanwei","family":"Hou","sequence":"additional","affiliation":[{"name":"Qinghai University , , ,","place":["Xining, Qinghai, China, 810016"]},{"name":"Qinghai Academy of Agricultural and Forestry Sciences , , ,","place":["Xining, Qinghai, China, 810016"]},{"name":"Laboratory of Research and Utilization of Crop Germplasm Resources on the Qinghai\u2212Tibet Plateau , , ,","place":["Xining, Qinghai, China, 810016"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ping","family":"Li","sequence":"additional","affiliation":[{"name":"Qinghai University , , ,","place":["Xining, Qinghai, China, 810016"]},{"name":"Qinghai Academy of Agricultural and Forestry Sciences , , ,","place":["Xining, Qinghai, China, 810016"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chengzhang","family":"Du","sequence":"additional","affiliation":[{"name":"Ministry of Agriculture and Rural Affairs (Co-construction by the Ministry and Province) , , ,","place":["Chongqing, China, 402160"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yujiao","family":"Liu","sequence":"additional","affiliation":[{"name":"Qinghai University , , ,","place":["Xining, Qinghai, China, 810016"]},{"name":"Laboratory of Research and Utilization of Crop Germplasm Resources on the Qinghai\u2212Tibet Plateau , , ,","place":["Xining, Qinghai, China, 810016"]}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"316","published-online":{"date-parts":[[2026,9,21]]},"reference":[{"key":"2026093009071279100_cit1","doi-asserted-by":"publisher","first-page":"110796","DOI":"10.1016\/j.lwt.2020.110796","article-title":"Nutritional composition, anti-nutritional\nfactors, pretreatments-cum-processing impact and food formulation\npotential of faba bean (Vicia faba L.): A comprehensive\nreview","volume":"138","author":"Rahate","year":"2021","journal-title":"LWT"},{"issue":"3","key":"2026093009071279100_cit2","doi-asserted-by":"publisher","first-page":"329","DOI":"10.1016\/j.fcr.2009.09.016","article-title":"Nutritional value of\nfaba bean (Vicia faba L.) seeds\nfor feed and food","volume":"115","author":"Crepon","year":"2010","journal-title":"Field Crops Res"},{"issue":"2","key":"2026093009071279100_cit3","doi-asserted-by":"publisher","first-page":"1647","DOI":"10.1016\/j.jksus.2019.12.024","article-title":"Morphological diversity of faba bean\n(Vicia faba L.) 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