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Results showed that QZTP3 was an acidic heteropolysaccharide with a molecular weight of 1.35 \u00d7 105 Da, primarily composed of galacturonic acid, arabinose, galactose, and rhamnose in a molar ratio of 34.19:20.31:13.94:13.84. QZTP3 possesses a complex backbone with \u21924)-\u03b1-D-GalpA--(1\u2192and\u21922,5)\u2192\u03b1-L-Araf-(1\u2192, among others, alongside side chains containing \u21923)-\u03b1-L-Araf-(1\u2192 and \u21925)-\u03b1-L-Araf-(1\u2192 units. Notably, QZTP3 demonstrated strong resistance to simulated gastrointestinal digestion. During in vitro fecal fermentation, QZTP3 stimulated the production of short-chain fatty acids, particularly acetate and propionate, which lowered pH. At the end of fermentation (48 h), the QZTP3 group exhibited higher relative abundances of beneficial genera (Bacteroides and Bifidobacterium) and lower relative abundances of putative pathogens (Escherichia-Shigella and Morganella). These findings support its potential application in functional foods and gut health supplements.<\/jats:p>","DOI":"10.1021\/acs.jafc.6c03886","type":"journal-article","created":{"date-parts":[[2026,7,23]],"date-time":"2026-07-23T05:53:15Z","timestamp":1784785995000},"page":"23868-23882","source":"Crossref","is-referenced-by-count":0,"title":["Structural Characterization,\n                    <i>In Vitro<\/i>\n                    Digestion,\nFermentation Properties, and Impact on Gut Microbiota\nof Polysaccharide from Qingzhuan Tea"],"prefix":"10.1021","volume":"74","author":[{"given":"Qianwei","family":"Ma","sequence":"first","affiliation":[{"name":"Shanghai Jiao Tong University , , 800 Dongchuan Road , Minhang District, ,","place":["Shanghai, China, 200240"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8302-8903","authenticated-orcid":true,"given":"Yang","family":"Wei","sequence":"additional","affiliation":[{"name":"Shanghai Jiao Tong University , , 800 Dongchuan Road , Minhang District, ,","place":["Shanghai, China, 200240"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Qitong","family":"Liu","sequence":"additional","affiliation":[{"name":"Shanghai Jiao Tong University , , 800 Dongchuan Road , Minhang District, ,","place":["Shanghai, China, 200240"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kang","family":"Wei","sequence":"additional","affiliation":[{"name":"Shanghai Jiao Tong University , , 800 Dongchuan Road , Minhang District, ,","place":["Shanghai, China, 200240"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zhonghua","family":"Liu","sequence":"additional","affiliation":[{"name":"Hunan Agricultural University , , ,","place":["Changsha, China, 410128"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7389-5330","authenticated-orcid":true,"given":"Xinlin","family":"Wei","sequence":"additional","affiliation":[{"name":"Shanghai Jiao Tong University , , 800 Dongchuan Road , Minhang District, ,","place":["Shanghai, China, 200240"]}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"316","published-online":{"date-parts":[[2026,7,23]]},"reference":[{"issue":"21","key":"2026080510093663400_cit1","doi-asserted-by":"publisher","first-page":"11837","DOI":"10.1021\/acs.jafc.3c08492","article-title":"Antidiabetic Potential\nof Tea and Its Active Compounds: From Molecular Mechanism to Clinical\nEvidence","volume":"72","author":"Wei","year":"2024","journal-title":"J. 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