{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,1]],"date-time":"2026-09-01T09:18:12Z","timestamp":1788254292375,"version":"build-2803163510"},"reference-count":45,"publisher":"American Chemical Society (ACS)","issue":"33","license":[{"start":{"date-parts":[[2026,8,12]],"date-time":"2026-08-12T00:00:00Z","timestamp":1786492800000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-029"},{"start":{"date-parts":[[2026,8,12]],"date-time":"2026-08-12T00:00:00Z","timestamp":1786492800000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-037"},{"start":{"date-parts":[[2026,8,12]],"date-time":"2026-08-12T00:00:00Z","timestamp":1786492800000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-045"}],"funder":[{"DOI":"10.13039\/100007540","name":"Jiangsu Agricultural Science and Technology Innovation Fund","doi-asserted-by":"publisher","award":["CX(24)3113"],"award-info":[{"award-number":["CX(24)3113"]}],"id":[{"id":"10.13039\/100007540","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["22478268"],"award-info":[{"award-number":["22478268"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/na","name":"Jiangsu Province Social Development General Project","doi-asserted-by":"publisher","award":["BE2023716"],"award-info":[{"award-number":["BE2023716"]}],"id":[{"id":"10.13039\/na","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/na","name":"Agricultural infrastructure project of Suzhou Science and Technology Development Plan","doi-asserted-by":"publisher","award":["SNG2022057"],"award-info":[{"award-number":["SNG2022057"]}],"id":[{"id":"10.13039\/na","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2026,8,26]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>Biomass polysaccharides are explored as inexpensive raw materials for preparing value-added products, such as mannan oligosaccharides (MOS). However, the varied composition of MOS and the underlying mechanism have not been studied thoroughly yet. Here a \u03b2-mannanase from Paenibacillus sp. was engineered for hydrolyzing biomass polysaccharides and producing prebiotic MOS. Structural analysis and alanine scanning revealed two hot spots, Q66 and T88, in the carbohydrate binding module (CBM). The mutant T88Y demonstrated a 65% activity improvement and a 5-fold thermal stability enhancement at 70 \u00b0C, and Q66F produced 2.2 g\/L of mannobiose and mannotriose from locust bean gum, with a 46% activity increase. Moreover, Q66F generated a 2.0 molar ratio of mannobiose\/mannotriose, and a reverse ratio of 0.4 was found in Q66R. The application of Q66R was further validated in the fermentation of citrus peel waste. The results provided efficient biocatalysts for producing prebiotic MOS from biomass polysaccharides and deeper comprehension of altering product chain length of \u03b2-mannanases from CBM.<\/jats:p>","DOI":"10.1021\/acs.jafc.6c03912","type":"journal-article","created":{"date-parts":[[2026,8,12]],"date-time":"2026-08-12T16:04:41Z","timestamp":1786550681000},"page":"26498-26509","source":"Crossref","is-referenced-by-count":0,"title":["Engineering CBM of \u03b2-Mannanase from\n                    <i>Paenibacillus<\/i>\n                    to Alter Product Chain Length for Preparing\nMannan Oligosaccharide Prebiotics"],"prefix":"10.1021","volume":"74","author":[{"given":"Ruiqi","family":"Fang","sequence":"first","affiliation":[{"name":"Suzhou University of Science and Technology , , ,","place":["Suzhou, P. R. China, 215009"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3659-0658","authenticated-orcid":true,"given":"JiaJia","family":"Chen","sequence":"additional","affiliation":[{"name":"Suzhou University of Science and Technology , , ,","place":["Suzhou, P. R. China, 215009"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yan","family":"Xu","sequence":"additional","affiliation":[{"name":"Suzhou University of Science and Technology , , ,","place":["Suzhou, P. R. China, 215009"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4088-0527","authenticated-orcid":true,"given":"Lishi","family":"Yan","sequence":"additional","affiliation":[{"name":"Suzhou University of Science and Technology , , ,","place":["Suzhou, P. R. China, 215009"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xin","family":"Ju","sequence":"additional","affiliation":[{"name":"Suzhou University of Science and Technology , , ,","place":["Suzhou, P. R. China, 215009"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7224-0973","authenticated-orcid":true,"given":"Liangzhi","family":"Li","sequence":"additional","affiliation":[{"name":"Suzhou University of Science and Technology , , ,","place":["Suzhou, P. R. China, 215009"]}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"316","published-online":{"date-parts":[[2026,8,12]]},"reference":[{"issue":"2","key":"2026090104471061500_cit1","doi-asserted-by":"publisher","DOI":"10.1111\/1541-4337.70144","article-title":"Citrus\nWastes as Sustainable Materials for Active and Intelligent Food Packaging:\nCurrent Advances","volume":"24","author":"Bertolo","year":"2025","journal-title":"Compr. Rev. Food Sci. Food\nSaf."},{"key":"2026090104471061500_cit2","doi-asserted-by":"publisher","first-page":"19","DOI":"10.1007\/978-1-0716-1822-6_2","article-title":"Sustainable Technological\nMethods for the Extraction of Phytochemicals from Citrus Byproducts","volume-title":"Plant Metabolic Engineering Methods and Protocols","author":"Multari","year":"2022"},{"issue":"1","key":"2026090104471061500_cit3","doi-asserted-by":"publisher","first-page":"9","DOI":"10.1007\/s13596-017-0292-8","article-title":"From Folk Medicine\nto Functional Food: A Review on the Bioactive Components and Pharmacological\nProperties of Citrus Peels","volume":"18","author":"Ademosun","year":"2018","journal-title":"Orient. Pharm. Exp.\nMed."},{"issue":"1","key":"2026090104471061500_cit4","doi-asserted-by":"publisher","first-page":"149","DOI":"10.1007\/s44187-024-00238-w","article-title":"Citrus Byproduct\nValorization: Pectin\nExtraction, Characterization, and Research Advances in Biomaterial\nDerivation for Applications in Active Film Packaging","volume":"4","author":"Rawat","year":"2024","journal-title":"Discovery Food"},{"key":"2026090104471061500_cit5","doi-asserted-by":"publisher","first-page":"121086","DOI":"10.1016\/j.fuel.2021.121086","article-title":"Lignocellulosic Biomass Based Biorefinery:\nA Successful Platform towards Circular Bioeconomy","volume":"302","author":"Rajesh Banu","year":"2021","journal-title":"Fuel"},{"key":"2026090104471061500_cit6","doi-asserted-by":"publisher","first-page":"1101","DOI":"10.1016\/j.biortech.2017.05.097","article-title":"Waste Biorefineries:\nEnabling Circular\nEconomies in Developing Countries","volume":"241","author":"Nizami","year":"2017","journal-title":"Bioresour.\nTechnol."},{"key":"2026090104471061500_cit7","doi-asserted-by":"publisher","first-page":"204","DOI":"10.1016\/j.apenergy.2012.03.066","article-title":"Bioethanol\nProduction from Mandarin (Citrus Unshiu) Peel Waste Using Popping\nPretreatment","volume":"102","author":"Choi","year":"2013","journal-title":"Appl. Energy"},{"issue":"6","key":"2026090104471061500_cit8","doi-asserted-by":"publisher","first-page":"3422","DOI":"10.1021\/jf903163t","article-title":"Ethanol\nProduction from Orange Peels: Two-Stage Hydrolysis and Fermentation\nStudies Using Optimized Parameters through Experimental Design","volume":"58","author":"Oberoi","year":"2010","journal-title":"J. Agric. Food Chem."},{"issue":"1","key":"2026090104471061500_cit9","doi-asserted-by":"publisher","first-page":"155","DOI":"10.1007\/s00253-023-12904-7","article-title":"Thermophilic Biocatalysts\nfor One-Step Conversion of\nCitrus Waste into Lactic Acid","volume":"108","author":"Aulitto","year":"2024","journal-title":"Appl. Microbiol.\nBiotechnol."},{"issue":"17","key":"2026090104471061500_cit10","doi-asserted-by":"publisher","first-page":"6834","DOI":"10.1021\/acssuschemeng.0c01735","article-title":"Valorization\nof Residual Orange Peels: Limonene Recovery, Volatile Fatty Acids,\nand Biogas Production","volume":"8","author":"Battista","year":"2020","journal-title":"ACS Sustainable Chem.\nEng."},{"issue":"1","key":"2026090104471061500_cit11","doi-asserted-by":"publisher","first-page":"43","DOI":"10.1007\/s13205-015-0362-x","article-title":"Evaluation\nof Orange Peel for Biosurfactant Production\nby Bacillus Licheniformis and Their Ability to Degrade Naphthalene\nand Crude Oil","volume":"6","author":"Kumar","year":"2016","journal-title":"3 Biotech"},{"issue":"5","key":"2026090104471061500_cit12","doi-asserted-by":"publisher","first-page":"1321","DOI":"10.1007\/s12010-020-03385-9","article-title":"Fermentative Production of Naringinase\nfrom Aspergillus Niger van Tieghem MTCC 2425 Using Citrus Wastes:\nProcess Optimization, Partial Purification, and Characterization","volume":"193","author":"Borkar","year":"2021","journal-title":"Appl. Biochem. Biotechnol."},{"key":"2026090104471061500_cit13","doi-asserted-by":"publisher","first-page":"706","DOI":"10.1016\/j.jclepro.2017.08.039","article-title":"Development of a Citrus\nPeel-Based Biorefinery Strategy\nfor the Production of Succinic Acid","volume":"166","author":"Patsalou","year":"2017","journal-title":"J. Clean.\nProd."},{"issue":"3","key":"2026090104471061500_cit14","doi-asserted-by":"publisher","first-page":"671","DOI":"10.1007\/s00253-010-2726-9","article-title":"Succinic Acid Production from Orange\nPeel and Wheat Straw by Batch Fermentations of Fibrobacter Succinogenes\nS85","volume":"88","author":"Li","year":"2010","journal-title":"Appl. Microbiol. Biotechnol."},{"key":"2026090104471061500_cit15","doi-asserted-by":"publisher","first-page":"103219","DOI":"10.1016\/j.copbio.2024.103219","article-title":"Sharp Solutions\nto Cleave Plant Fibers","volume":"90","author":"Jacobson","year":"2024","journal-title":"Curr. Opin. Biotechnol."},{"issue":"D1","key":"2026090104471061500_cit16","doi-asserted-by":"publisher","first-page":"D490","DOI":"10.1093\/nar\/gkt1178","article-title":"The Carbohydrate-Active\nEnzymes Database (CAZy) in 2013","volume":"42","author":"Lombard","year":"2014","journal-title":"Nucleic Acids\nRes."},{"issue":"4","key":"2026090104471061500_cit17","doi-asserted-by":"publisher","first-page":"130","DOI":"10.1007\/s11274-024-03912-4","article-title":"Thermophilic \u03b2-Mannanases from Bacteria: Production,\nResources, Structural Features and Bioengineering Strategies","volume":"40","author":"Sadaqat","year":"2024","journal-title":"World J. Microbiol. Biotechnol."},{"key":"2026090104471061500_cit18","doi-asserted-by":"publisher","first-page":"107840","DOI":"10.1016\/j.fbio.2025.107840","article-title":"\u03b2-Mannanase\nand Its Application\nin Prebiotic (Mannooligosaccharides) Preparation","volume":"74","author":"Pongsapipatana","year":"2025","journal-title":"Food Biosci."},{"issue":"28","key":"2026090104471061500_cit19","doi-asserted-by":"publisher","first-page":"10221","DOI":"10.1080\/10408398.2023.2222165","article-title":"Functional\n\u03b2-Mannooligosaccharides:\nSources, Enzymatic Production and Application as Prebiotics","volume":"64","author":"Rana","year":"2024","journal-title":"Crit. Rev. Food Sci. Nutr."},{"key":"2026090104471061500_cit20","doi-asserted-by":"publisher","first-page":"308","DOI":"10.1016\/j.biortech.2018.07.143","article-title":"Production\nOptimization and Characterization of Mannooligosaccharide Generating\n\u03b2-Mannanase from Aspergillus Oryzae","volume":"268","author":"Jana","year":"2018","journal-title":"Bioresour.\nTechnol."},{"issue":"5","key":"2026090104471061500_cit21","doi-asserted-by":"publisher","first-page":"443","DOI":"10.1016\/j.jbiosc.2020.07.001","article-title":"Engineering\nof \u03b2-Mannanase from Aspergillus Niger to Increase Product Selectivity\ntowards Medium Chain Length Mannooligosaccharides","volume":"130","author":"Arunrattanamook","year":"2020","journal-title":"J. Biosci. Bioeng."},{"issue":"13","key":"2026090104471061500_cit22","doi-asserted-by":"publisher","first-page":"2827","DOI":"10.1021\/acs.jafc.7b00123","article-title":"Structural Characterization\nand in Vitro Fermentation of \u03b2-Mannooligosaccharides Produced\nfrom Locust Bean Gum by GH-26 Endo-\u03b2-1,4-Mannanase (ManB-1601)","volume":"65","author":"Srivastava","year":"2017","journal-title":"J. Agric. Food Chem."},{"issue":"5","key":"2026090104471061500_cit23","doi-asserted-by":"publisher","first-page":"1919","DOI":"10.1007\/s00253-022-11832-2","article-title":"A Novel Neutral Thermophilic \u03b2-Mannanase\nfrom Malbranchea Cinnamomea\nfor Controllable Production of Partially Hydrolyzed Konjac Powder","volume":"106","author":"Li","year":"2022","journal-title":"Appl. Microbiol. Biotechnol."},{"key":"2026090104471061500_cit24","doi-asserted-by":"publisher","first-page":"130373","DOI":"10.1016\/j.biortech.2024.130373","article-title":"Production\nof Mannooligosaccharides from Orange Peel Waste with \u03b2-Mannanase\nExpressed in Trichosporonoides Oedocephalis","volume":"395","author":"Zhou","year":"2024","journal-title":"Bioresour. Technol."},{"issue":"7","key":"2026090104471061500_cit25","doi-asserted-by":"publisher","first-page":"815","DOI":"10.1002\/jobm.202200080","article-title":"Enhanced Extracellular\n\u03b2-Mannanase Production by Overexpressing\nPrsA Lipoprotein in Bacillus Subtilis and Optimizing Culture Conditions","volume":"62","author":"Xu","year":"2022","journal-title":"J. Basic Microbiol."},{"issue":"8016","key":"2026090104471061500_cit26","doi-asserted-by":"publisher","first-page":"493","DOI":"10.1038\/s41586-024-07487-w","article-title":"Accurate Structure\nPrediction of\nBiomolecular Interactions with AlphaFold 3","volume":"630","author":"Abramson","year":"2024","journal-title":"Nature"},{"issue":"10","key":"2026090104471061500_cit27","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pcbi.1002708","article-title":"CAVER 3.0:\nA Tool for the Analysis of Transport Pathways in Dynamic Protein Structures","volume":"8","author":"Chovancova","year":"2012","journal-title":"PLoS Comput. Biol."},{"issue":"20","key":"2026090104471061500_cit28","doi-asserted-by":"publisher","first-page":"14624","DOI":"10.1074\/jbc.M113.459438","article-title":"Structural and Biochemical\nAnalyses of Glycoside Hydrolase\nFamilies 5 and 26 \u0392b-(1,4)-Mannanases from Podospora Anserina\nReveal Differences upon Manno-Oligosaccharide Catalysis","volume":"288","author":"Couturier","year":"2013","journal-title":"J. Biol. Chem."},{"issue":"30","key":"2026090104471061500_cit29","doi-asserted-by":"publisher","first-page":"18853","DOI":"10.1021\/acs.jafc.5c03371","article-title":"Protein Engineering\nof an Inositol-1-Phosphate Synthase Based on\nthe Active-Site Redesign Facilitates the Biomanufacturing of Myo-Inositol\nfrom Starch via In Vitro Synthetic Enzymatic Biosystem","volume":"73","author":"Fan","year":"2025","journal-title":"J. Agric. Food Chem."},{"issue":"32","key":"2026090104471061500_cit30","doi-asserted-by":"publisher","first-page":"13204","DOI":"10.1002\/anie.201901491","article-title":"The Crucial\nRole of Methodology Development in Directed Evolution of Selective\nEnzymes","volume":"59","author":"Qu","year":"2020","journal-title":"Angew. Chem., Int. Ed."},{"issue":"7","key":"2026090104471061500_cit31","doi-asserted-by":"publisher","DOI":"10.1128\/aem.00888-24","article-title":"Functional\nStudies on Tandem Carbohydrate-Binding Modules of a Multimodular Enzyme\nPossessing Two Catalytic Domains","volume":"90","author":"Liu","year":"2024","journal-title":"Appl. Environ.\nMicrobiol."},{"issue":"10","key":"2026090104471061500_cit32","doi-asserted-by":"publisher","first-page":"269","DOI":"10.1007\/s13205-022-03339-4","article-title":"Enhancement\nof Catalytic Activity and Alkaline Stability of Cellobiohydrolase\nby Structure-Based Protein Engineering","volume":"12","author":"Prabmark","year":"2022","journal-title":"3 Biotech"},{"key":"2026090104471061500_cit33","doi-asserted-by":"publisher","first-page":"99","DOI":"10.1016\/j.enzmictec.2016.08.003","article-title":"Genome\nMining and Motif Truncation\nof Glycoside Hydrolase Family 5 Endo-\u03b2-1,4-Mannanase Encoded\nby Aspergillus Oryzae RIB40 for Potential Konjac Flour Hydrolysis\nor Feed Additive","volume":"93\u201394","author":"Tang","year":"2016","journal-title":"Enzyme. Microb. Technol."},{"key":"2026090104471061500_cit34","doi-asserted-by":"publisher","first-page":"131275","DOI":"10.1016\/j.ijbiomac.2024.131275","article-title":"The CBM91\nModule Enhances the Activity of \u03b2-Xylosidase\/\u03b1-L-Arabinofuranosidase\nPphXyl43B from Paenibacillus Physcomitrellae XB by Adopting a Unique\nLoop Conformation at the Top of the Active Pocket","volume":"266","author":"Pang","year":"2024","journal-title":"Int. J. Biol. Macromol."},{"issue":"12","key":"2026090104471061500_cit35","doi-asserted-by":"publisher","first-page":"2298","DOI":"10.1080\/09168451.2016.1230003","article-title":"Production of High Activity Aspergillus\nNiger BCC4525 \u03b2-Mannanase in Pichia Pastoris and Its Application\nfor Mannooligosaccharides Production from Biomass Hydrolysis","volume":"80","author":"Harnpicharnchai","year":"2016","journal-title":"Biosci., Biotechnol., Biochem."},{"key":"2026090104471061500_cit36","doi-asserted-by":"publisher","first-page":"23","DOI":"10.1016\/j.pep.2018.01.009","article-title":"Kinetic Properties\nAnalysis of Beta-Mannanase from\nKlebsiella Oxytoca KUB-CW2\u20133 Expressed in Escherichia Coli","volume":"146","author":"Tuntrakool","year":"2018","journal-title":"Protein Expression Purif."},{"key":"2026090104471061500_cit37","doi-asserted-by":"publisher","first-page":"1119232","DOI":"10.3389\/fmicb.2023.1119232","article-title":"Thermostability Modification of \u03b2-Mannanase from\nAspergillus Niger via Flexibility Modification Engineering","volume":"14","author":"Tan","year":"2023","journal-title":"Front. Microbiol."},{"issue":"9","key":"2026090104471061500_cit38","doi-asserted-by":"publisher","first-page":"3500","DOI":"10.1021\/acssuschemeng.3c06266","article-title":"Supercharging\nCarbohydrate Binding\nModule Alone Enhances Endocellulase Thermostability, Binding, and\nActivity on Cellulosic Biomass","volume":"12","author":"DeChellis","year":"2024","journal-title":"ACS Sustainable\nChem. Eng."},{"key":"2026090104471061500_cit39","doi-asserted-by":"publisher","first-page":"348","DOI":"10.1016\/j.foodchem.2017.04.159","article-title":"Structural\nCharacterization\nand Evaluation of Prebiotic Activity of Oil Palm Kernel Cake Mannanoligosaccharides","volume":"234","author":"Kalidas","year":"2017","journal-title":"Food Chem."},{"key":"2026090104471061500_cit40","doi-asserted-by":"publisher","first-page":"125709","DOI":"10.1016\/j.foodchem.2019.125709","article-title":"Preparation,\nCharacterization, and Prebiotic Activity of Manno-Oligosaccharides\nProduced from Cassia Gum by a Glycoside Hydrolase Family 134 \u03b2-Mannanase","volume":"309","author":"Li","year":"2020","journal-title":"Food Chem."},{"issue":"2","key":"2026090104471061500_cit41","doi-asserted-by":"publisher","first-page":"111","DOI":"10.1007\/s12033-014-9807-4","article-title":"Recovery and\nPurification of Oligosaccharides from Copra Meal by Recombinant Endo-\u03b2-Mannanase\nand Deciphering Molecular Mechanism Involved and Its Role as Potent\nTherapeutic Agent","volume":"57","author":"Ghosh","year":"2015","journal-title":"Mol. Biotechnol."},{"key":"2026090104471061500_cit42","doi-asserted-by":"publisher","first-page":"64","DOI":"10.1016\/j.ejbt.2020.11.001","article-title":"Enzymatic\nPreparation of Manno-Oligosaccharides\nfrom Locust Bean Gum and Palm Kernel Cake, and Investigations into\nIts Prebiotic Activity","volume":"49","author":"Zhang","year":"2021","journal-title":"Electron. J. Biotechnol."},{"issue":"3","key":"2026090104471061500_cit43","doi-asserted-by":"publisher","first-page":"535","DOI":"10.1016\/j.jmb.2008.03.068","article-title":"From Structure to\nFunction: Insights into the Catalytic Substrate Specificity and Thermostability\nDisplayed by Bacillus Subtilis Mannanase BCman","volume":"379","author":"Yan","year":"2008","journal-title":"J. Mol. Biol."},{"issue":"3","key":"2026090104471061500_cit44","doi-asserted-by":"publisher","first-page":"1027","DOI":"10.1042\/bj20021860","article-title":"The Modular\nArchitecture of Cellvibrio\nJaponicus Mannanases in Glycoside Hydrolase Families 5 and 26 Points\nto Differences in Their Role in Mannan Degradation","volume":"371","author":"Hogg","year":"2003","journal-title":"Biochem. J."},{"issue":"1\u20132","key":"2026090104471061500_cit45","doi-asserted-by":"publisher","first-page":"86","DOI":"10.1080\/10242420701788835","article-title":"Kinetics and Stereochemistry of the\nCellulomonas Fimi \u03b2-Mannanase Studied Using 1H-NMR","volume":"26","author":"Anderson","year":"2008","journal-title":"Biocatal. Biotransform."}],"container-title":["Journal of Agricultural and Food Chemistry"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/pubs.acs.org\/doi\/pdf\/10.1021\/acs.jafc.6c03912","content-type":"application\/pdf","content-version":"vor","intended-application":"unspecified"},{"URL":"https:\/\/pubs.acs.org\/jafcau\/article-pdf\/74\/33\/26498\/66516130\/acs.jafc.6c03912.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"syndication"},{"URL":"https:\/\/pubs.acs.org\/jafcau\/article-pdf\/74\/33\/26498\/66516130\/acs.jafc.6c03912.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,9,1]],"date-time":"2026-09-01T08:47:31Z","timestamp":1788252451000},"score":1,"resource":{"primary":{"URL":"https:\/\/pubs.acs.org\/jafcau\/article\/74\/33\/26498\/5253687\/Engineering-CBM-of-Mannanase-from-Paenibacillus-to"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,8,12]]},"references-count":45,"journal-issue":{"issue":"33","published-online":{"date-parts":[[2026,8,12]]},"published-print":{"date-parts":[[2026,8,26]]}},"URL":"https:\/\/doi.org\/10.1021\/acs.jafc.6c03912","relation":{},"ISSN":["0021-8561","1520-5118"],"issn-type":[{"value":"0021-8561","type":"print"},{"value":"1520-5118","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026,8,12]]}}}