{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,5]],"date-time":"2026-08-05T15:09:48Z","timestamp":1785942588728,"version":"3.56.0"},"reference-count":90,"publisher":"American Chemical Society (ACS)","issue":"30","license":[{"start":{"date-parts":[[2026,7,21]],"date-time":"2026-07-21T00:00:00Z","timestamp":1784592000000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-029"},{"start":{"date-parts":[[2026,7,21]],"date-time":"2026-07-21T00:00:00Z","timestamp":1784592000000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-037"},{"start":{"date-parts":[[2026,7,21]],"date-time":"2026-07-21T00:00:00Z","timestamp":1784592000000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-045"}],"funder":[{"DOI":"10.13039\/501100002873","name":"Chulalongkorn University","doi-asserted-by":"publisher","award":["FOOD_FF_68_103_3000_018"],"award-info":[{"award-number":["FOOD_FF_68_103_3000_018"]}],"id":[{"id":"10.13039\/501100002873","id-type":"DOI","asserted-by":"publisher"}]},{"name":"BIOTEC","award":["P-17-51846"],"award-info":[{"award-number":["P-17-51846"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2026,8,5]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>Enterotoxigenic Escherichia coli (ETEC) causes significant mortality and economic losses in piglet production. This study aimed to identify a safe, effective probiotic for E. coli control under practical conditions. Using a green fluorescent protein-tagged ETEC screening platform, we identified three Lactobacillus johnsonii (LJ) strains with potent antimicrobial activity. LJ FBU1718 demonstrated superior efficacy against multidrug-resistant ETEC, high gastrointestinal tolerance, and strong mucin adhesion. In IPEC-J2 cells, LJ FBU1718 reduced ETEC-induced inflammation, preserved epithelial integrity, and decreased cytotoxicity. Whole-genome sequencing confirmed its safety, showing an absence of mobile antimicrobial resistance elements. In suckling piglets, LJ FBU1718 supplementation improved growth and survival while enhancing fecal consistency. These benefits were underpinned by gut microbiota modulation, where Lactobacillus enrichment and E. coli reduction correlated with a significant decrease in diarrheal incidence. These findings establish LJ FBU1718 as a safe, practical probiotic candidate to enhance productivity in swine production.<\/jats:p>","DOI":"10.1021\/acs.jafc.6c04192","type":"journal-article","created":{"date-parts":[[2026,7,21]],"date-time":"2026-07-21T20:05:41Z","timestamp":1784664341000},"page":"23779-23794","source":"Crossref","is-referenced-by-count":0,"title":["A Genomically Safe\n                    <i>Lactobacillus johnsonii<\/i>\n                    Lacking Mobile Antimicrobial\nResistance Genes Suppresses\n                    <i>Escherichia coli<\/i>\n                    and Modulates Gut Microbiota and\nDiarrhea Incidence in Suckling Piglets"],"prefix":"10.1021","volume":"74","author":[{"given":"Mattika","family":"Abhisingha","sequence":"first","affiliation":[{"name":"National Center for Genetic Engineering and Biotechnology (BIOTEC), National Science and Technology Development Agency (NSTDA) , ,","place":["Pathumthani, Thailand, 12120"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kanittha","family":"Chantarasakha","sequence":"additional","affiliation":[{"name":"National Center for Genetic Engineering and Biotechnology (BIOTEC), National Science and Technology Development Agency (NSTDA) , ,","place":["Pathumthani, Thailand, 12120"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Pannita","family":"Santiyanont","sequence":"additional","affiliation":[{"name":"National Center for Genetic Engineering and Biotechnology (BIOTEC), National Science and Technology Development Agency (NSTDA) , ,","place":["Pathumthani, Thailand, 12120"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Manadsaree","family":"Klomtun","sequence":"additional","affiliation":[{"name":"National Center for Genetic Engineering and Biotechnology (BIOTEC), National Science and Technology Development Agency (NSTDA) , ,","place":["Pathumthani, Thailand, 12120"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chetsadaporn","family":"Pitaksutheepong","sequence":"additional","affiliation":[{"name":"National Center for Genetic Engineering and Biotechnology (BIOTEC), National Science and Technology Development Agency (NSTDA) , ,","place":["Pathumthani, Thailand, 12120"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Suwimon","family":"Boonrungsiman","sequence":"additional","affiliation":[{"name":"National Science and Technology Development Agency (NSTDA) , , ,","place":["Pathumthani, Thailand, 12120"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sawannee","family":"Sutheeworapong","sequence":"additional","affiliation":[{"name":"King Mongkut\u2019s University of Technology Thonburi , , ,","place":["Bangkok, Thailand, 10150"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Saharuetai","family":"Jeamsripong","sequence":"additional","affiliation":[{"name":"Chulalongkorn University , , ,","place":["Bangkok, Thailand, 10330"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Cherdpong","family":"Phupolphan","sequence":"additional","affiliation":[{"name":"Chulalongkorn University , , ,","place":["Nakhon Pathom, Thailand, 73000"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Wonnop","family":"Visessanguan","sequence":"additional","affiliation":[{"name":"Mahidol University , , ,","place":["Bangkok, Thailand, 10700"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5315-2249","authenticated-orcid":true,"given":"Surapun","family":"Tepaamorndech","sequence":"additional","affiliation":[{"name":"Chulalongkorn University and King Chulalongkorn Memorial Hospital , , ,","place":["Bangkok, Thailand, 10330"]}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"316","published-online":{"date-parts":[[2026,7,21]]},"reference":[{"issue":"4","key":"2026080510084198500_cit1","doi-asserted-by":"publisher","first-page":"742","DOI":"10.5713\/ab.23.0303","article-title":"Invited Review- Current status, challenges and prospects for pig\nproduction in Asia","volume":"37","author":"Wang","year":"2024","journal-title":"Anim Biosci"},{"key":"2026080510084198500_cit2","unstructured":"Organisation for Economic Co-operation and Development (OECD) and Food and Agriculture Organization of the United Nations (FAO).\n          \n          Agricultural\nOutlook 2024\u20132033, July 2024."},{"key":"2026080510084198500_cit3","doi-asserted-by":"publisher","first-page":"297","DOI":"10.1016\/j.aninu.2024.03.006","article-title":"Nutrition\nstrategies to control post-weaning diarrhea of piglets: From the perspective\nof feeds","volume":"17","author":"Tang","year":"2024","journal-title":"Anim. Nutr."},{"issue":"11","key":"2026080510084198500_cit4","doi-asserted-by":"publisher","first-page":"2434","DOI":"10.3390\/v14112434","article-title":"Porcine Epidemic\nDiarrhea Virus: An Updated Overview of Virus Epidemiology, Virulence\nVariation Patterns and Virus\u2013Host Interactions","volume":"14","author":"Zhang","year":"2022","journal-title":"Viruses"},{"issue":"5","key":"2026080510084198500_cit5","doi-asserted-by":"publisher","first-page":"2722","DOI":"10.1111\/tbed.14039","article-title":"African Swine Fever\nspread across Asia, 2018\u20132019","volume":"68","author":"Mighell","year":"2021","journal-title":"Transbound.\nEmerg. Dis"},{"issue":"4","key":"2026080510084198500_cit6","doi-asserted-by":"publisher","first-page":"682","DOI":"10.3390\/antibiotics12040682","article-title":"Swine Colibacillosis:\nGlobal Epidemiologic and Antimicrobial Scenario","volume":"12","author":"Barros","year":"2023","journal-title":"Antibiotics"},{"key":"2026080510084198500_cit7","doi-asserted-by":"publisher","DOI":"10.29011\/2688948x.100136","article-title":"Enterotoxigenic\nE. coli (ETEC) and Porcine Epidemic Diarrhea Virus (PEDV) Diarrheal\nInfections and Resistance in Piglets","author":"Augustino","year":"2022","journal-title":"Archives\nof Environmental Sciences and Environmental Toxicology"},{"issue":"1","key":"2026080510084198500_cit8","doi-asserted-by":"publisher","first-page":"20","DOI":"10.1186\/s40813-016-0039-9","article-title":"Prevalence\nof virulence factors in\nenterotoxigenic Escherichia coli isolated from pigs\nwith post-weaning diarrhoea in Europe","volume":"2","author":"Luppi","year":"2016","journal-title":"Porcine\nHealth Manag"},{"issue":"1","key":"2026080510084198500_cit9","doi-asserted-by":"publisher","first-page":"24","DOI":"10.3390\/toxins11010024","article-title":"Heat-Stable\nEnterotoxins of Enterotoxigenic Escherichia coli and\nTheir Impact on Host Immunity","volume":"11","author":"Wang","year":"2019","journal-title":"Toxins"},{"key":"2026080510084198500_cit10","doi-asserted-by":"publisher","first-page":"1077","DOI":"10.2147\/IDR.S354884","article-title":"Gut Commensal Escherichia coli, a High-Risk Reservoir of Transferable\nPlasmid-Mediated Antimicrobial Resistance Traits","volume":"15","author":"Tawfick","year":"2022","journal-title":"Infect Drug Resist"},{"key":"2026080510084198500_cit11","doi-asserted-by":"publisher","first-page":"740927","DOI":"10.1016\/j.aquaculture.2024.740927","article-title":"Insights into antimicrobial and multidrug\nresistance of Escherichia coli in hybrid red tilapia\ncultivation","volume":"588","author":"Thaotumpitak","year":"2024","journal-title":"Aquaculture"},{"issue":"8","key":"2026080510084198500_cit12","doi-asserted-by":"publisher","DOI":"10.1128\/aem.00749-24","article-title":"Host species\nshapes genotype, antimicrobial resistance, and virulence profiles\nof enterotoxigenic Escherichia coli (ETEC) from livestock\nin the United States","volume":"90","author":"Fu","year":"2024","journal-title":"Appl. Environ. Microbiol."},{"issue":"7","key":"2026080510084198500_cit13","doi-asserted-by":"publisher","first-page":"1188","DOI":"10.3390\/foods14071188","article-title":"Prevalence, Molecular Characterization, and\nAntimicrobial Resistance\nProfile of Enterotoxigenic Escherichia coli Isolates\nfrom Pig Farms in China","volume":"14","author":"Zhu","year":"2025","journal-title":"Foods"},{"issue":"1","key":"2026080510084198500_cit14","doi-asserted-by":"publisher","first-page":"2469","DOI":"10.1038\/s41467-025-56825-7","article-title":"The future of antibiotic use in livestock","volume":"16","author":"Acosta","year":"2025","journal-title":"Nat. Commun."},{"issue":"12","key":"2026080510084198500_cit15","doi-asserted-by":"publisher","DOI":"10.15252\/embr.202051034","article-title":"Antibiotic\nresistance and persistence\u2500Implications for human health and\ntreatment perspectives","volume":"21","author":"Huemer","year":"2020","journal-title":"EMBO Rep."},{"key":"2026080510084198500_cit16","doi-asserted-by":"publisher","first-page":"1070340","DOI":"10.3389\/fmicb.2023.1070340","article-title":"A longitudinal study\nreveals persistence of antimicrobial resistance on livestock farms\nis not due to antimicrobial usage alone","volume":"14","author":"Smith","year":"2023","journal-title":"Front.\nMicrobiol."},{"issue":"1","key":"2026080510084198500_cit17","doi-asserted-by":"publisher","first-page":"9","DOI":"10.1038\/s44259-024-00026-7","article-title":"Co-selection for\nantibiotic resistance\nby environmental contaminants","volume":"2","author":"Murray","year":"2024","journal-title":"NPJ. Antimicrob\nResist"},{"issue":"1","key":"2026080510084198500_cit18","doi-asserted-by":"publisher","first-page":"34","DOI":"10.3390\/pathogens4010034","article-title":"The Use of Lactic Acid Bacteria as\na Probiotic in Swine Diets","volume":"4","author":"Yang","year":"2015","journal-title":"Pathogens"},{"issue":"1","key":"2026080510084198500_cit19","doi-asserted-by":"publisher","first-page":"184","DOI":"10.1007\/s12602-018-9511-y","article-title":"Probiotic Lactobacillus johnsonii BS15 Promotes\nGrowth Performance, Intestinal\nImmunity, and Gut Microbiota in Piglets","volume":"12","author":"Xin","year":"2020","journal-title":"Probiotics\nAntimicrob. Prot."},{"issue":"3","key":"2026080510084198500_cit20","doi-asserted-by":"publisher","first-page":"493","DOI":"10.3390\/ani14030493","article-title":"Lactobacillus johnsonii Improves Intestinal\nBarrier Function and Reduces Post-Weaning Diarrhea in Piglets: Involvement\nof the Endocannabinoid System","volume":"14","author":"Yin","year":"2024","journal-title":"Animals"},{"key":"2026080510084198500_cit21","doi-asserted-by":"publisher","first-page":"100292","DOI":"10.1016\/j.vas.2023.100292","article-title":"Detection of antimicrobial\nresistance\ngenes in Lactobacillus spp. from poultry probiotic\nproducts and their horizontal transfer among Escherichia coli","volume":"20","author":"Rokon-Uz-Zaman","year":"2023","journal-title":"Vet. Anim. Sci."},{"issue":"1","key":"2026080510084198500_cit22","doi-asserted-by":"publisher","DOI":"10.1128\/spectrum.00019-24","article-title":"Evaluating the health risk of probiotic supplements from the perspective\nof antimicrobial resistance","volume":"13","author":"Tian","year":"2025","journal-title":"Microbiol. Spectr."},{"issue":"2","key":"2026080510084198500_cit23","doi-asserted-by":"publisher","first-page":"218","DOI":"10.1007\/s12602-017-9304-8","article-title":"Selection\nof Potential Probiotic Lactobacillus with Inhibitory\nActivity Against Salmonella and Fecal Coliform Bacteria","volume":"10","author":"Abhisingha","year":"2018","journal-title":"Probiot. antimicrob.\nproteins."},{"key":"2026080510084198500_cit24","first-page":"115","volume-title":"16S\/23S rRNA sequencing","author":"Lane","year":"1991"},{"issue":"7","key":"2026080510084198500_cit25","doi-asserted-by":"publisher","first-page":"4782","DOI":"10.1128\/AEM.02885-05","article-title":"Comparison\nof Virulence Gene Profiles of Escherichia coli Strains\nIsolated from Healthy and Diarrheic Swine","volume":"72","author":"Chapman","year":"2006","journal-title":"Appl.\nEnviron. Microbiol."},{"issue":"1","key":"2026080510084198500_cit26","doi-asserted-by":"publisher","first-page":"25","DOI":"10.1177\/104063870902100104","article-title":"Design and Evaluation of a Multiplex Polymerase Chain\nReaction Assay for the Simultaneous Identification of Genes for Nine\nDifferent Virulence Factors Associated with Escherichia Coli that Cause Diarrhea and Edema Disease in Swine","volume":"21","author":"Casey","year":"2009","journal-title":"J. Vet. Diagn. Invest."},{"key":"2026080510084198500_cit27","volume-title":"Performance Standards for\nAntimicrobial Disk Susceptibility Tests, CLSI Standard M02","author":"Clinical and Laboratory Standards Institute.","year":"2025","edition":"14"},{"key":"2026080510084198500_cit28","volume-title":"Performance Standards for\nAntimicrobial Susceptibility Testing, CLSI Standard M100","author":"Clinical and Laboratory Standards Institute.","year":"2025"},{"key":"2026080510084198500_cit29","doi-asserted-by":"publisher","DOI":"10.2903\/sp.efsa.2018.en-1389","article-title":"Guidance\non the characterisation of microorganisms used as feed additives or\nas production organisms","author":"European Food Safety Authority.","year":"2018","journal-title":"European Food Safety\nAuthority"},{"key":"2026080510084198500_cit30","volume-title":"Performance Standards for\nAntimicrobial Susceptibility testing. CLSI Standard M100","author":"Clinical and Laboratory Standards Institute.","year":"2025"},{"issue":"12","key":"2026080510084198500_cit31","doi-asserted-by":"publisher","first-page":"8982","DOI":"10.1128\/AEM.71.12.8982-8986.2005","article-title":"Evaluation of New Broth Media for Microdilution Antibiotic Susceptibility\nTesting of Lactobacilli, Pediococci, Lactococci,\nand Bifidobacteria","volume":"71","author":"Klare","year":"2005","journal-title":"Appl. Environ.\nMicrobiol."},{"issue":"2","key":"2026080510084198500_cit32","doi-asserted-by":"publisher","DOI":"10.14814\/phy2.13514","article-title":"Lactobacillus reuteri strains protect epithelial barrier integrity of IPEC-J2\nmonolayers from the detrimental effect of enterotoxigenic Escherichia coli","volume":"6","author":"Karimi","year":"2018","journal-title":"Physiol. Rep."},{"issue":"1","key":"2026080510084198500_cit33","doi-asserted-by":"publisher","first-page":"76","DOI":"10.1186\/s40104-020-00488-5","article-title":"Lactobacillus salivarius alleviates inflammation via NF-\u03baB signaling\nin ETEC K88-induced\nIPEC-J2 cells","volume":"11","author":"Qiao","year":"2020","journal-title":"J. anim. sci. biotechnol."},{"key":"2026080510084198500_cit34","doi-asserted-by":"publisher","DOI":"10.20935\/acadbiol7982","article-title":"Effects\nof probiotic lactobacilli on inflammatory responses\nto Escherichia coli in pig intestinal cells","volume":"3","author":"Uezen","year":"2025","journal-title":"Academia\nBiology"},{"issue":"6","key":"2026080510084198500_cit35","doi-asserted-by":"publisher","first-page":"1113","DOI":"10.1039\/C3FO60702J","article-title":"A standardised static in vitro digestion\nmethod suitable for food \u2013 an international consensus","volume":"5","author":"Minekus","year":"2014","journal-title":"Food Funct"},{"issue":"5","key":"2026080510084198500_cit36","doi-asserted-by":"publisher","first-page":"1156","DOI":"10.2527\/jas.2007-0145","article-title":"Evaluation of a dynamic in vitro model to simulate\nthe porcine ileal digestion of diets differing in carbohydrate composition1","volume":"86","author":"Meunier","year":"2008","journal-title":"J. Anim. Sci."},{"issue":"2","key":"2026080510084198500_cit37","doi-asserted-by":"publisher","first-page":"485","DOI":"10.1111\/jam.12539","article-title":"In vitro evaluation\nof the mucin-adhesion ability and probiotic potential of Lactobacillus mucosae LM1","volume":"117","author":"Valeriano","year":"2014","journal-title":"J. Appl. Microbiol."},{"issue":"6","key":"2026080510084198500_cit38","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pcbi.1005595","article-title":"Resolving bacterial\ngenome assemblies from short and long sequencing reads","volume":"13","author":"Wick","year":"2017","journal-title":"PLoS Comput. Biol."},{"issue":"14","key":"2026080510084198500_cit39","doi-asserted-by":"publisher","first-page":"2068","DOI":"10.1093\/bioinformatics\/btu153","article-title":"Prokka: rapid\nprokaryotic genome annotation","volume":"30","author":"Seemann","year":"2014","journal-title":"Bioinformatics"},{"issue":"45","key":"2026080510084198500_cit40","doi-asserted-by":"publisher","first-page":"19126","DOI":"10.1073\/pnas.0906412106","article-title":"Shifting the\ngenomic gold standard\nfor the prokaryotic species definition","volume":"106","author":"Richter","year":"2009","journal-title":"Proc.\nNatl. Acad. Sci. U.S.A."},{"issue":"8","key":"2026080510084198500_cit41","doi-asserted-by":"publisher","first-page":"1308","DOI":"10.5713\/ajas.17.0746","article-title":"Effect of Lactobacillus salivarius on growth performance, diarrhea\nincidence, fecal bacterial population and intestinal morphology of\nsuckling pigs challenged with F4+ enterotoxigenic Escherichia coli","volume":"31","author":"Sayan","year":"2018","journal-title":"Asian-Australas.\nJ. Anim. Sci."},{"issue":"11","key":"2026080510084198500_cit42","doi-asserted-by":"publisher","first-page":"10709","DOI":"10.3168\/jds.2020-18907","article-title":"Technical note:\nIs fecal consistency scoring an accurate measure of fecal dry matter\nin dairy calves?","volume":"103","author":"Renaud","year":"2020","journal-title":"JDS"},{"issue":"1","key":"2026080510084198500_cit43","doi-asserted-by":"publisher","first-page":"96","DOI":"10.1186\/s12866-020-01781-z","article-title":"Design of PCR assays to specifically detect and identify\n37 Lactobacillus species in a single 96 well plate","volume":"20","author":"Kim","year":"2020","journal-title":"BMC Microbiol."},{"key":"2026080510084198500_cit44","doi-asserted-by":"publisher","first-page":"456","DOI":"10.1016\/j.watres.2019.03.011","article-title":"Standardized data quality acceptance criteria\nfor a rapid Escherichia coli qPCR method (Draft Method\nC) for water quality monitoring at recreational beaches","volume":"156","author":"Sivaganesan","year":"2019","journal-title":"Water Res."},{"issue":"4","key":"2026080510084198500_cit45","doi-asserted-by":"publisher","first-page":"212","DOI":"10.1089\/fpd.2015.2064","article-title":"Real-Time\nTaqMan PCR Assay for the\nDetection of Heat-Labile and Heat-Stable Enterotoxin Genes in a Geographically\nDiverse Collection of Enterotoxigenic Escherichia coli Strains and Stool Specimens","volume":"13","author":"Pattabiraman","year":"2016","journal-title":"FPD"},{"issue":"1","key":"2026080510084198500_cit46","doi-asserted-by":"publisher","first-page":"257","DOI":"10.1099\/00221287-148-1-257","article-title":"Determination of bacterial\nload by real-time PCR using a broad-range (universal) probe and primers\nset","volume":"148","author":"Nadkarni","year":"2002","journal-title":"Microbiology"},{"issue":"3","key":"2026080510084198500_cit47","doi-asserted-by":"publisher","first-page":"351","DOI":"10.1016\/j.mimet.2011.06.010","article-title":"Improvement\nof phylum- and class-specific primers for real-time PCR quantification\nof bacterial taxa","volume":"86","author":"Bacchetti De Gregoris","year":"2011","journal-title":"J. Microbiol. Methods"},{"issue":"2","key":"2026080510084198500_cit48","doi-asserted-by":"publisher","first-page":"205","DOI":"10.1007\/s13167-018-0132-z","article-title":"Specific\nproperties of probiotic strains: relevance and benefits for the host","volume":"9","author":"Bubnov","year":"2018","journal-title":"EPMA Journal"},{"key":"2026080510084198500_cit49","doi-asserted-by":"publisher","first-page":"106126","DOI":"10.1016\/j.mimet.2020.106126","article-title":"Functional in vitro screening of probiotic\nstrains for inoculation of piglets as a prophylactic measure towards\nEnterotoxigenic Escherichia coli infection","volume":"180","author":"Hansen","year":"2021","journal-title":"J. Microbiol. Methods"},{"key":"2026080510084198500_cit50","doi-asserted-by":"publisher","first-page":"104163","DOI":"10.1016\/j.micpath.2020.104163","article-title":"In vitro probiotic properties of Pediococcus pentosaceus L1 and its effects on enterotoxigenic Escherichia coli-induced inflammatory responses in porcine\nintestinal epithelial cells","volume":"144","author":"Yin","year":"2020","journal-title":"Microb. Pathog."},{"issue":"1","key":"2026080510084198500_cit51","doi-asserted-by":"publisher","first-page":"96","DOI":"10.1038\/s41522-025-00734-6","article-title":"Evolutionary\nadaptation of probiotics in the gut: selection pressures, optimization\nstrategies, and regulatory challenges","volume":"11","author":"Leeflang","year":"2025","journal-title":"npj Biofilms\nand Microbiomes"},{"key":"2026080510084198500_cit52","doi-asserted-by":"publisher","first-page":"1364","DOI":"10.3389\/fmicb.2018.01364","article-title":"Swine-Derived\nProbiotic Lactobacillus plantarum Inhibits Growth\nand Adhesion of\nEnterotoxigenic Escherichia coli and Mediates Host\nDefense","volume":"9","author":"Wang","year":"2018","journal-title":"Front Microbiol"},{"issue":"1","key":"2026080510084198500_cit53","doi-asserted-by":"publisher","first-page":"2653","DOI":"10.1038\/s41467-021-22849-y","article-title":"Variability of plasmid fitness effects contributes\nto plasmid persistence in bacterial communities","volume":"12","author":"Alonso-del Valle","year":"2021","journal-title":"Nat. Commun."},{"key":"2026080510084198500_cit54","doi-asserted-by":"crossref","DOI":"10.1007\/978-3-319-16104-4","volume-title":"The Impact of Food Bioactives on Health: in vitro\nand ex vivo models","author":"Verhoeckx","year":"2015"},{"issue":"1","key":"2026080510084198500_cit55","doi-asserted-by":"publisher","first-page":"39","DOI":"10.1309\/GJ98-JAH8-VQ57-D6N0","article-title":"Interleukin-8-An Early Marker for\nBacterial Infection","volume":"31","author":"Hirao","year":"2000","journal-title":"Lab. Med."},{"issue":"8","key":"2026080510084198500_cit56","doi-asserted-by":"publisher","first-page":"470","DOI":"10.1007\/s00203-022-03097-0","article-title":"Safety\nevaluation and anti-inflammatory activity of Lactobacillus johnsonii IDCC 9203 isolated from feces of breast-fed infants","volume":"204","author":"Adedeji","year":"2022","journal-title":"Arch. Microbiol."},{"key":"2026080510084198500_cit57","doi-asserted-by":"publisher","first-page":"320","DOI":"10.1016\/j.crfs.2021.04.006","article-title":"In vitro test to\nevaluate survival in the gastrointestinal tract of commercial probiotics","volume":"4","author":"Naissinger da Silva","year":"2021","journal-title":"Curr. res. nutr. food sci."},{"issue":"5","key":"2026080510084198500_cit58","doi-asserted-by":"publisher","first-page":"448","DOI":"10.1016\/j.tim.2023.11.001","article-title":"Colonization factors of human and\nanimal-specific enterotoxigenic Escherichia coli (ETEC)","volume":"32","author":"von Mentzer","year":"2024","journal-title":"Trends Microbiol."},{"issue":"1","key":"2026080510084198500_cit59","doi-asserted-by":"publisher","first-page":"178","DOI":"10.1128\/JB.00822-08","article-title":"Heat-Labile Enterotoxin Promotes Escherichia coli Adherence to Intestinal Epithelial Cells","volume":"191","author":"Johnson","year":"2009","journal-title":"J. Bacteriol."},{"key":"2026080510084198500_cit60","doi-asserted-by":"publisher","first-page":"104271","DOI":"10.1016\/j.micpath.2020.104271","article-title":"Antimicrobial and antiadhesive effects of Lactobacillus isolates of healthy human gut origin on Enterotoxigenic Escherichia coli (ETEC) and Enteroaggregative Escherichia coli (EAEC)","volume":"148","author":"Pazhoohan","year":"2020","journal-title":"Microb. Pathog."},{"issue":"2","key":"2026080510084198500_cit61","doi-asserted-by":"publisher","first-page":"243","DOI":"10.1007\/s12602-017-9273-y","article-title":"Roles of Probiotic Lactobacilli Inclusion in Helping Piglets Establish Healthy\nIntestinal Inter-environment for Pathogen Defense","volume":"10","author":"Yang","year":"2018","journal-title":"Probiot. antimicrob. proteins."},{"issue":"1","key":"2026080510084198500_cit62","doi-asserted-by":"publisher","first-page":"2185034","DOI":"10.1080\/19490976.2023.2185034","article-title":"Emerging issues in probiotic\nsafety: 2023 perspectives","volume":"15","author":"Merenstein","year":"2023","journal-title":"Gut Microbes"},{"issue":"2","key":"2026080510084198500_cit63","doi-asserted-by":"publisher","first-page":"148","DOI":"10.3390\/antibiotics13020148","article-title":"Microbiological\nQuality and Antimicrobial Resistance of Commercial Probiotic Products\nfor Food-Producing Animals","volume":"13","author":"Tran","year":"2024","journal-title":"Antibiotics"},{"issue":"29","key":"2026080510084198500_cit64","doi-asserted-by":"publisher","first-page":"11481","DOI":"10.1074\/jbc.RA118.003989","article-title":"Structural mechanism\nof AadA, a dual-specificity aminoglycoside adenylyltransferase from Salmonella enterica","volume":"293","author":"Stern","year":"2018","journal-title":"J. Biol. Chem."},{"issue":"5","key":"2026080510084198500_cit65","doi-asserted-by":"publisher","first-page":"2902","DOI":"10.1111\/jam.15738","article-title":"To be, or not to be\u2019-The\ndilemma of \u2018silent\u2019\nantimicrobial resistance genes in bacteria","volume":"133","author":"Deekshit","year":"2022","journal-title":"J. Appl. Microbiol."},{"issue":"6","key":"2026080510084198500_cit66","doi-asserted-by":"publisher","first-page":"1464","DOI":"10.1111\/j.1365-2958.2011.07657.x","article-title":"Activation of cryptic aminoglycoside resistance in Salmonella enterica","volume":"80","author":"Koskiniemi","year":"2011","journal-title":"Mol. Microbiol."},{"issue":"12","key":"2026080510084198500_cit67","doi-asserted-by":"publisher","first-page":"1491","DOI":"10.3109\/14767058.2014.958462","article-title":"A new risk\nfactor for neonatal vancomycin-resistant Enterococcus colonisation:\nbacterial probiotics","volume":"28","author":"Topcuoglu","year":"2015","journal-title":"J. Matern Fetal Neonatal\nMed."},{"issue":"11","key":"2026080510084198500_cit68","doi-asserted-by":"publisher","first-page":"5340","DOI":"10.1128\/JCM.41.11.5340-5343.2003","article-title":"Outbreak of Saccharomyces cerevisiae Subtype boulardii Fungemia in\nPatients Neighboring Those Treated with a Probiotic Preparation of\nthe Organism","volume":"41","author":"Cassone","year":"2003","journal-title":"J. Clin. Microbiol."},{"issue":"6","key":"2026080510084198500_cit69","doi-asserted-by":"publisher","first-page":"1527","DOI":"10.1007\/s10096-025-05115-7","article-title":"Alkalihalobacillus clausii bacteremia after probiotic use for acute diarrhea:\na case report","volume":"44","author":"Gallego Aristizabal","year":"2025","journal-title":"Eur. J. Clin Microbiol Infect\nDis"},{"key":"2026080510084198500_cit70","doi-asserted-by":"publisher","DOI":"10.12659\/ajcr.947564","article-title":"Whole-Genome\nSequencing of Blood-Isolated Lactobacillus johnsonii in Thailand: Clinical Implications and Public Health Relevance","volume":"26","author":"Chukamnerd","year":"2025","journal-title":"Am. J. Case Rep."},{"issue":"5","key":"2026080510084198500_cit71","doi-asserted-by":"publisher","first-page":"900","DOI":"10.1093\/jac\/dkm035","article-title":"Antimicrobial susceptibilities of Lactobacillus, Pediococcus and Lactococcus human isolates and cultures intended for probiotic or nutritional\nuse","volume":"59","author":"Klare","year":"2007","journal-title":"J. Antimicrob. Chemother."},{"key":"2026080510084198500_cit72","doi-asserted-by":"publisher","DOI":"10.2903\/j.efsa.2012.2740","article-title":"Guidance\non the assessment of bacterial susceptibility to antimicrobials of\nhuman and veterinary importance, EFSA Panel on Additives and Products\nor Substances used in Animal Feed","author":"European Food Safety Authority.","year":"2012","journal-title":"European\nFood Safety Authority"},{"issue":"1","key":"2026080510084198500_cit73","doi-asserted-by":"publisher","first-page":"16","DOI":"10.1186\/s40813-017-0063-4","article-title":"Swine enteric\ncolibacillosis: diagnosis, therapy and antimicrobial resistance","volume":"3","author":"Luppi","year":"2017","journal-title":"Porc. Health Manag."},{"issue":"6","key":"2026080510084198500_cit79","doi-asserted-by":"publisher","first-page":"923","DOI":"10.1017\/S0007114514003201","article-title":"Effect of\nbovine colostrum feeding in comparison with milk replacer and natural\nfeeding on the immune responses and colonisation of enterotoxigenic Escherichia coli in the intestinal tissue of piglets","volume":"113","author":"Sugiharto","year":"2015","journal-title":"Br. J. Nutr."},{"key":"2026080510084198500_cit80","doi-asserted-by":"publisher","first-page":"1098818","DOI":"10.3389\/fmicb.2023.1098818","article-title":"Changyanning regulates\ngut microbiota and metabolism to ameliorate intestinal injury induced\nby ETEC K88","volume":"14","author":"Guo","year":"2023","journal-title":"Front. Microbiol."},{"issue":"1","key":"2026080510084198500_cit81","doi-asserted-by":"publisher","first-page":"7210","DOI":"10.1038\/s41598-022-11340-3","article-title":"Microencapsulated\nprobiotic Lactiplantibacillus plantarum and\/or Pediococcus acidilactici strains ameliorate diarrhoea in\npiglets challenged with enterotoxigenic Escherichia coli","volume":"12","author":"Pupa","year":"2022","journal-title":"Sci. Rep."},{"key":"2026080510084198500_cit74","doi-asserted-by":"publisher","first-page":"101741","DOI":"10.1016\/j.fbio.2022.101741","article-title":"Recent developments\nof lactic acid bacteria and their metabolites on foodborne pathogens\nand spoilage bacteria: Facts and gaps","volume":"47","author":"Fidan","year":"2022","journal-title":"Food\nBiosci"},{"issue":"18","key":"2026080510084198500_cit75","doi-asserted-by":"publisher","first-page":"8317","DOI":"10.1007\/s00253-013-5113-5","article-title":"The damaging effects\nof short chain fatty acids on Escherichia coli membranes","volume":"97","author":"Royce","year":"2013","journal-title":"Appl. Microbiol. Biotechnol."},{"key":"2026080510084198500_cit82","doi-asserted-by":"publisher","first-page":"976406","DOI":"10.3389\/fmicb.2022.976406","article-title":"Potential\nof gut-derived short-chain fatty acids to control enteric pathogens","volume":"13","author":"Zhan","year":"2022","journal-title":"Front Microbiol"},{"key":"2026080510084198500_cit83","doi-asserted-by":"publisher","first-page":"47","DOI":"10.1016\/j.intimp.2017.07.023","article-title":"Sodium butyrate\ninhibits the NF-kappa B signaling pathway\nand histone deacetylation, and attenuates experimental colitis in\nan IL-10 independent manner","volume":"51","author":"Lee","year":"2017","journal-title":"Int. Immunopharmacol."},{"key":"2026080510084198500_cit84","doi-asserted-by":"publisher","first-page":"650313","DOI":"10.3389\/fphys.2021.650313","article-title":"Use of Short-Chain\nFatty Acids for the Recovery of the Intestinal Epithelial Barrier\nAffected by Bacterial Toxins","volume":"12","author":"Pe\u0301rez-Reytor","year":"2021","journal-title":"Front. Physiol."},{"issue":"1","key":"2026080510084198500_cit85","doi-asserted-by":"publisher","first-page":"3745135","DOI":"10.1155\/2022\/3745135","article-title":"Sodium Butyrate Ameliorates Oxidative\nStress-Induced Intestinal Epithelium Barrier Injury and Mitochondrial\nDamage through AMPK-Mitophagy Pathway","volume":"2022","author":"Li","year":"2022","journal-title":"Oxid.\nMed. Cell. Longev."},{"issue":"9","key":"2026080510084198500_cit76","doi-asserted-by":"publisher","first-page":"1289","DOI":"10.3390\/ani15091289","article-title":"Butyrate Derivatives\nExhibited Anti-Inflammatory Effects and Enhanced Intestinal Barrier\nIntegrity in Porcine Cell Culture Models","volume":"15","author":"Kovanda","year":"2025","journal-title":"Animals"},{"issue":"2","key":"2026080510084198500_cit77","doi-asserted-by":"publisher","first-page":"2281011","DOI":"10.1080\/19490976.2023.2281011","article-title":"Butyrate reduces adherent-invasive E. coli-evoked\ndisruption of epithelial mitochondrial morphology\nand barrier function: involvement of free fatty acid receptor 3","volume":"15","author":"Hamed","year":"2023","journal-title":"Gut Microbes"},{"key":"2026080510084198500_cit78","doi-asserted-by":"publisher","first-page":"109448","DOI":"10.1016\/j.abb.2022.109448","article-title":"Sodium\nbutyrate exerts antioxidant stress effects and attenuates A\u03b225\u201335-induced cytotoxicity in PC12 cells","volume":"731","author":"Yuan","year":"2022","journal-title":"Arch. Biochem. Biophys."},{"issue":"17","key":"2026080510084198500_cit86","doi-asserted-by":"publisher","first-page":"E3914","DOI":"10.1073\/pnas.1717522115","article-title":"Tradeoffs\nbetween immune function and childhood growth among Amazonian forager-horticulturalists","volume":"115","author":"Urlacher","year":"2018","journal-title":"Proc. Natl. Acad. Sci. U. S. A."},{"key":"2026080510084198500_cit87","doi-asserted-by":"crossref","DOI":"10.5772\/34067","article-title":"The Impact of\nProbiotics on the Gastrointestinal Physiology","volume-title":"New Advances in the Basic and Clinical Gastroenterology","author":"Matur","year":"2012"},{"issue":"4","key":"2026080510084198500_cit88","doi-asserted-by":"publisher","first-page":"1107","DOI":"10.3390\/nu12041107","article-title":"The Effect\nof Probiotics on the Production\nof Short-Chain Fatty Acids by Human Intestinal Microbiome","volume":"12","author":"Markowiak-Kopec\u0301","year":"2020","journal-title":"Nutrients"},{"key":"2026080510084198500_cit89","doi-asserted-by":"publisher","first-page":"105977","DOI":"10.1016\/j.fbio.2025.105977","article-title":"Lactobacillus paracasei GF027 promotes growth and cognitive development\nin juvenile rats via regulating intestinal microecology and nourishing\nthe brain","volume":"65","author":"Yang","year":"2025","journal-title":"Food Biosci"},{"issue":"5","key":"2026080510084198500_cit90","doi-asserted-by":"publisher","first-page":"785","DOI":"10.3390\/ani13050785","article-title":"Effects of Bacillus licheniformis and\nCombination of Probiotics and Enzymes as Supplements on Growth Performance\nand Serum Parameters in Early-Weaned Grazing Yak Calves","volume":"13","author":"Zhou","year":"2023","journal-title":"Animals"}],"container-title":["Journal of Agricultural\nand Food Chemistry"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/pubs.acs.org\/doi\/pdf\/10.1021\/acs.jafc.6c04192","content-type":"application\/pdf","content-version":"vor","intended-application":"unspecified"},{"URL":"https:\/\/pubs.acs.org\/jafcau\/article-pdf\/74\/30\/23779\/66095554\/acs.jafc.6c04192.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"syndication"},{"URL":"https:\/\/pubs.acs.org\/jafcau\/article-pdf\/74\/30\/23779\/66095554\/acs.jafc.6c04192.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,8,5]],"date-time":"2026-08-05T14:31:58Z","timestamp":1785940318000},"score":1,"resource":{"primary":{"URL":"https:\/\/pubs.acs.org\/jafcau\/article\/74\/30\/23779\/5225131\/A-Genomically-Safe-Lactobacillus-johnsonii-Lacking"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,7,21]]},"references-count":90,"journal-issue":{"issue":"30","published-online":{"date-parts":[[2026,7,21]]},"published-print":{"date-parts":[[2026,8,5]]}},"URL":"https:\/\/doi.org\/10.1021\/acs.jafc.6c04192","relation":{},"ISSN":["0021-8561","1520-5118"],"issn-type":[{"value":"0021-8561","type":"print"},{"value":"1520-5118","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026,7,21]]}}}