{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,16]],"date-time":"2026-09-16T14:07:39Z","timestamp":1789567659793,"version":"build-2803163510"},"reference-count":33,"publisher":"American Chemical Society (ACS)","issue":"36","license":[{"start":{"date-parts":[[2026,9,3]],"date-time":"2026-09-03T00:00:00Z","timestamp":1788393600000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-029"},{"start":{"date-parts":[[2026,9,3]],"date-time":"2026-09-03T00:00:00Z","timestamp":1788393600000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-037"},{"start":{"date-parts":[[2026,9,3]],"date-time":"2026-09-03T00:00:00Z","timestamp":1788393600000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-045"}],"funder":[{"DOI":"10.13039\/501100025476","name":"Faculty of Science, Kasetsart University","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100025476","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100005621","name":"Kasetsart University Research and Development Institute","doi-asserted-by":"publisher","award":["FF(KU)14.67"],"award-info":[{"award-number":["FF(KU)14.67"]}],"id":[{"id":"10.13039\/501100005621","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2026,9,16]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>Tropomyosin is a pan-allergen in crustaceans known to cross-react with homologous proteins from edible insects. However, epitope-level cross-reactivity in edible insects remains poorly defined. This study aimed to identify IgE-binding epitopes of tropomyosin and to evaluate their cross-reactivity between shrimp and the edible two-spotted cricket. Here, sera from shrimp-allergic patients were screened for IgE-binding to proteins from Pacific white shrimp (Penaeus vannamei) and two-spotted cricket (Gryllus bimaculatus). Candidate tropomyosin epitopes from the two-spotted cricket were predicted. The cross-reactivity of tropomyosin was assessed using an inhibition dot blot assay, inhibition ELISA, and LAD2 cell degranulation assay. Approximately 40% of shrimp-allergic patients showed IgE reactivity to crude proteins from shrimp and the two-spotted cricket. Four identified peptide epitopes induced \u03b2-hexosaminidase release from LAD2 cells. These findings provide insights into the molecular basis of tropomyosin cross-reactivity essential for the development of biomarker-based tools for allergen risk assessment in the food industry.<\/jats:p>","DOI":"10.1021\/acs.jafc.6c04758","type":"journal-article","created":{"date-parts":[[2026,9,4]],"date-time":"2026-09-04T04:24:26Z","timestamp":1788495866000},"page":"28712-28722","source":"Crossref","is-referenced-by-count":0,"title":["IgE Epitope Mapping\nof Cross-Reactive Tropomyosin\nbetween Shrimp and Edible Cricket (\n                    <i>Gryllus bimaculatus<\/i>\n                    )"],"prefix":"10.1021","volume":"74","author":[{"ORCID":"https:\/\/orcid.org\/0009-0009-4879-208X","authenticated-orcid":true,"given":"Siriluk","family":"Khumsui","sequence":"first","affiliation":[{"name":"Kasetsart University , , ,","place":["Bangkok, Thailand, 10900"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kunlanan","family":"Thuraphan","sequence":"additional","affiliation":[{"name":"Kasetsart University , , ,","place":["Bangkok, Thailand, 10900"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tran Thi Cam","family":"Lien","sequence":"additional","affiliation":[{"name":"Can Tho University , , ,","place":["Can Tho City, Vietnam, 90000"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Pharima","family":"Phiriyangkul","sequence":"additional","affiliation":[{"name":"Kamphaeng Saen Campus, Kasetsart University , , ,","place":["Nakhon Pathom, Thailand, 73140"]},{"name":"Kasetsart University , , ,","place":["Bangkok, Thailand, 10900"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yiwa","family":"Suksawat","sequence":"additional","affiliation":[{"name":"Phramongkutklao Hospital , , ,","place":["Bangkok, Thailand, 10400"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chama","family":"Inson","sequence":"additional","affiliation":[{"name":"Kasetsart University , , ,","place":["Bangkok, Thailand, 10900"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chotika","family":"Yokthongwattana","sequence":"additional","affiliation":[{"name":"Kasetsart University , , ,","place":["Bangkok, Thailand, 10900"]},{"name":"Kasetsart University , , ,","place":["Bangkok, Thailand, 10900"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2432-2122","authenticated-orcid":true,"given":"Chomdao","family":"Sinthuvanich","sequence":"additional","affiliation":[{"name":"Kasetsart University , , ,","place":["Bangkok, Thailand, 10900"]},{"name":"Kasetsart University , , ,","place":["Bangkok, Thailand, 10900"]}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"316","published-online":{"date-parts":[[2026,9,3]]},"reference":[{"issue":"3","key":"2026091609095569400_cit1","doi-asserted-by":"publisher","first-page":"576","DOI":"10.1016\/j.jaci.2023.11.918","article-title":"Feast for\nthought: A comprehensive\nreview of food allergy 2021\u20132023","volume":"153","author":"Bartha","year":"2024","journal-title":"J.\nAllergy Clin. Immunol."},{"issue":"5","key":"2026091609095569400_cit2","doi-asserted-by":"publisher","DOI":"10.1007\/s11882-021-01012-z","article-title":"Allergens\nfrom edible insects: Cross-reactivity and effects of processing","volume":"21","author":"De Marchi","year":"2021","journal-title":"Curr. Allergy Asthma Rep."},{"key":"2026091609095569400_cit3","doi-asserted-by":"publisher","DOI":"10.1016\/j.foodchem.2021.130231","article-title":"Identification\nof potential\nallergens in larva, pupa, moth, silk, slough and feces of domestic\nsilkworm (Bombyx mori)","volume":"362","author":"He","year":"2021","journal-title":"Food Chem."},{"issue":"3","key":"2026091609095569400_cit4","doi-asserted-by":"publisher","DOI":"10.3390\/foods8030095","article-title":"Quality aspects of\ninsects as food\u2500nutritional, sensory, and related concepts","volume":"8","author":"Elhassan","year":"2019","journal-title":"Foods"},{"key":"2026091609095569400_cit5","doi-asserted-by":"publisher","first-page":"1","DOI":"10.1016\/j.ifset.2012.11.005","article-title":"Potential and challenges of insects as an innovative\nsource for food and feed production","volume":"17","author":"Rumpold","year":"2013","journal-title":"Innov.\nFood Sci. Emerging Technol."},{"key":"2026091609095569400_cit6","doi-asserted-by":"publisher","DOI":"10.1016\/j.foodcont.2023.110036","article-title":"Factors affecting\nhistamine levels in edible crickets: Implications for post-harvest\nmanagement and farmed cricket production","volume":"154","author":"Chumjai","year":"2023","journal-title":"Food\nControl"},{"issue":"2","key":"2026091609095569400_cit7","doi-asserted-by":"publisher","first-page":"655","DOI":"10.63369\/ijat.2025.21.2.655-672","article-title":"Species of commercially-farmed crickets\nin Thailand","volume":"21","author":"Ruang-Rit","year":"2025","journal-title":"Int. J. Agric. Technol."},{"issue":"7","key":"2026091609095569400_cit8","doi-asserted-by":"publisher","first-page":"4342","DOI":"10.4049\/jimmunol.162.7.4342","article-title":"Molecular Characterization of American Cockroach Tropomyosin\n(Periplaneta americana Allergen 7),\na Cross-Reactive Allergen","volume":"162","author":"Asturias","year":"1999","journal-title":"J. Immunol."},{"issue":"5","key":"2026091609095569400_cit9","doi-asserted-by":"publisher","first-page":"370","DOI":"10.1002\/mnfr.200400015","article-title":"Recombinant\ntropomyosin from Penaeus aztecus (rPen\na 1) for measurement of specific immuno-globulin E antibodies relevant\nin food allergy to crustaceans and other invertebrates","volume":"48","author":"DeWitt","year":"2004","journal-title":"Mol. Nutr. Food Res."},{"issue":"14","key":"2026091609095569400_cit10","doi-asserted-by":"publisher","first-page":"4031","DOI":"10.1016\/j.foodchem.2013.06.105","article-title":"Impact of heat processing\non the detection of the major shellfish allergen tropomyosin in crustaceans\nand molluscs using specific monoclonal antibodies","volume":"141","author":"Kamath","year":"2013","journal-title":"Food Chem."},{"issue":"2","key":"2026091609095569400_cit11","doi-asserted-by":"publisher","first-page":"e74","DOI":"10.2500\/ar.2012.3.0036","article-title":"Group 10\nallergens (tropomyosins) from house-dust mites may cause covariation\nof sensitization to allergens from other invertebrates","volume":"3","author":"Shafique","year":"2012","journal-title":"Allergy Rhinol."},{"issue":"29","key":"2026091609095569400_cit12","doi-asserted-by":"publisher","first-page":"8247","DOI":"10.1021\/acs.jafc.1c00934","article-title":"Molecular\nCharacterization and Cross-Allergenicity of Tropomyosin from Freshwater\nCrustaceans","volume":"69","author":"Laurchan","year":"2021","journal-title":"J. Agric. Food Chem."},{"key":"2026091609095569400_cit13","doi-asserted-by":"publisher","DOI":"10.1016\/j.fochms.2021.100049","article-title":"Isolation and proteomic characterization of tropomyosin\nextracted from edible insect protein","volume":"3","author":"Hall","year":"2021","journal-title":"Food Chem.\nMol. Sci."},{"issue":"19","key":"2026091609095569400_cit14","doi-asserted-by":"publisher","DOI":"10.3390\/foods11192931","article-title":"Recent insight on edible insect protein: Extraction, functional properties,\nallergenicity, bioactivity, and applications","volume":"11","author":"Pan","year":"2022","journal-title":"Foods"},{"key":"2026091609095569400_cit15","doi-asserted-by":"publisher","DOI":"10.1016\/j.foodcont.2024.110328","article-title":"Effect of\nmicrowave vacuum drying and tray drying on the allergenicity of protein\nallergens in edible cricket, Gryllus bimaculatus","volume":"160","author":"Khammeethong","year":"2024","journal-title":"Food Control"},{"issue":"7","key":"2026091609095569400_cit16","doi-asserted-by":"publisher","first-page":"210","DOI":"10.1007\/s40629-016-0124-2","article-title":"Allergens and molecular diagnostics\nof shellfish allergy: Part 22 of the Series Molecular Allergology","volume":"25","author":"Lopata","year":"2016","journal-title":"Allergo J. Int."},{"issue":"1","key":"2026091609095569400_cit17","doi-asserted-by":"publisher","DOI":"10.1016\/j.waojou.2018.10.001","article-title":"Edible insects: Cross-recognition\nof IgE from crustacean-and house dust mite allergic patients, and\nreduction of allergenicity by food processing","volume":"12","author":"Pali-Scho\u0308ll","year":"2019","journal-title":"World Allergy Org. J."},{"key":"2026091609095569400_cit18","doi-asserted-by":"publisher","first-page":"127","DOI":"10.1016\/j.molimm.2018.12.015","article-title":"Cross-allergenicity\nof crustacean\nand the edible insect Gryllus bimaculatus in patients with shrimp\nallergy","volume":"106","author":"Kamemura","year":"2019","journal-title":"Mol. Immunol."},{"issue":"1","key":"2026091609095569400_cit19","doi-asserted-by":"publisher","first-page":"38","DOI":"10.1039\/d1fo01867a","article-title":"Epitope\nmapping and the effects of\nvarious factors on the immunoreactivity of main allergens in egg white","volume":"13","author":"Gazme","year":"2022","journal-title":"Food Funct."},{"key":"2026091609095569400_cit20","doi-asserted-by":"publisher","DOI":"10.1016\/j.foodchem.2019.125603","article-title":"IgE-binding epitope mapping of tropomyosin\nallergen (Exo m 1) from Exopalaemon modestus, the freshwater Siberian prawn","volume":"309","author":"Zhang","year":"2020","journal-title":"Food Chem."},{"issue":"4","key":"2026091609095569400_cit21","doi-asserted-by":"publisher","first-page":"840","DOI":"10.1271\/bbb.80751","article-title":"Molecular cloning and\nallergenicity of Pen j 1, a major\nallergen of kuruma prawn, Penaeus japonicus","volume":"73","author":"Kunimoto","year":"2009","journal-title":"Biosci., Biotechnol., Biochem."},{"issue":"11","key":"2026091609095569400_cit22","doi-asserted-by":"publisher","first-page":"2944","DOI":"10.1021\/acs.jafc.7b06042","article-title":"Identification\nof Allergenic Epitopes and Critical Amino Acids of Major Allergens\nin Chinese Shrimp (Penaeus chinensis) by Immunoinformatics\nCoupled with Competitive-Binding Strategy","volume":"66","author":"Fu","year":"2018","journal-title":"J.\nAgric. Food Chem."},{"key":"2026091609095569400_cit23","doi-asserted-by":"publisher","first-page":"7.38.1","DOI":"10.1002\/0471142735.im0738s91","article-title":"Measuring\nmast cell mediator release","volume":"91","author":"Kuehn","year":"2010","journal-title":"Curr. Protoc. Immunol."},{"issue":"1","key":"2026091609095569400_cit24","doi-asserted-by":"publisher","first-page":"27","DOI":"10.1159\/000048166","article-title":"Identification\nof continuous, allergenic\nregions of the major shrimp allergen Pen a 1 (tropomyosin)","volume":"127","author":"Ayuso","year":"2002","journal-title":"Int. Arch. Allergy Immunol."},{"issue":"2\u20134","key":"2026091609095569400_cit25","doi-asserted-by":"publisher","first-page":"300","DOI":"10.1159\/000024108","article-title":"IgE\u2013Binding\nEpitopes of Shrimp Tropomyosin, the Major Allergen Pen a 1","volume":"118","author":"Reese","year":"1999","journal-title":"Int. Arch. Allergy Immunol."},{"key":"2026091609095569400_cit26","doi-asserted-by":"publisher","DOI":"10.1016\/j.foodchem.2020.126763","article-title":"Analysis\nof the\nallergenicity and B cell epitopes in tropomyosin of shrimp (Litopenaeus vannamei) and correlation to cross-reactivity\nbased on epitopes with fish (Larimichthys crocea)\nand clam (Ruditapes philippinarum)","volume":"323","author":"Xu","year":"2020","journal-title":"J. Agric. Food Chem."},{"key":"2026091609095569400_cit27","doi-asserted-by":"publisher","first-page":"44739","DOI":"10.1074\/jbc.M111.277152","article-title":"Mas-related\ngene X2 (MrgX2) is a novel G protein-coupled receptor for the antimicrobial\npeptide LL-37 in human mast cells: Resistance to receptor phosphorylation,\ndesensitization, and internalization","volume":"286","author":"Subramanian","year":"2011","journal-title":"J. Biol.\nChem."},{"issue":"4","key":"2026091609095569400_cit28","doi-asserted-by":"publisher","first-page":"1033","DOI":"10.1016\/j.jaci.2024.07.002","article-title":"Inhibition\nof mast cell degranulation by novel small molecule MRGPRX2 antagonists","volume":"154","author":"Wollam","year":"2024","journal-title":"J. Allergy Clin. Immunol."},{"key":"2026091609095569400_cit29","doi-asserted-by":"publisher","DOI":"10.1155\/2016\/6760830","article-title":"An introduction\nto B-cell epitope\nmapping and in silico epitope prediction","volume":"2016","author":"Potocnakova","year":"2016","journal-title":"J.\nImmunol. Res."},{"issue":"1","key":"2026091609095569400_cit30","doi-asserted-by":"publisher","first-page":"127","DOI":"10.1111\/1541-4337.12889","article-title":"An overview\nof tropomyosin as an\nimportant seafood allergen: Structure, cross-reactivity, epitopes,\nallergenicity, and processing modifications","volume":"21","author":"Cheng","year":"2022","journal-title":"Compr. Rev. Food Sci. Food Saf."},{"issue":"38","key":"2026091609095569400_cit31","doi-asserted-by":"publisher","first-page":"12189","DOI":"10.1021\/acs.jafc.2c04849","article-title":"IgE Epitope\nAnalysis for Scy p 1 and Scy p 3, the Heat-Stable Myofibrillar Allergens\nin Mud Crab","volume":"70","author":"Li","year":"2022","journal-title":"J. Agric. Food Chem."},{"issue":"9","key":"2026091609095569400_cit32","doi-asserted-by":"publisher","DOI":"10.3390\/molecules27092667","article-title":"In silico prediction of cross-reactive\nepitopes of tropomyosin from shrimp and other arthropods involved\nin allergy","volume":"27","author":"Saetang","year":"2022","journal-title":"Molecules"},{"issue":"5","key":"2026091609095569400_cit33","doi-asserted-by":"publisher","first-page":"1019","DOI":"10.1111\/all.14120","article-title":"Cross-reactivities\nof non-homologous allergens","volume":"75","author":"Bublin","year":"2020","journal-title":"Allergy"}],"container-title":["Journal of Agricultural\nand Food Chemistry"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/pubs.acs.org\/doi\/pdf\/10.1021\/acs.jafc.6c04758","content-type":"application\/pdf","content-version":"vor","intended-application":"unspecified"},{"URL":"https:\/\/pubs.acs.org\/jafcau\/article-pdf\/74\/36\/28712\/68334470\/acs.jafc.6c04758.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"syndication"},{"URL":"https:\/\/pubs.acs.org\/jafcau\/article-pdf\/74\/36\/28712\/68334470\/acs.jafc.6c04758.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,9,16]],"date-time":"2026-09-16T13:31:02Z","timestamp":1789565462000},"score":1,"resource":{"primary":{"URL":"https:\/\/pubs.acs.org\/jafcau\/article\/74\/36\/28712\/5416728\/IgE-Epitope-Mapping-of-Cross-Reactive-Tropomyosin"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,9,3]]},"references-count":33,"journal-issue":{"issue":"36","published-online":{"date-parts":[[2026,9,3]]},"published-print":{"date-parts":[[2026,9,16]]}},"URL":"https:\/\/doi.org\/10.1021\/acs.jafc.6c04758","relation":{},"ISSN":["0021-8561","1520-5118"],"issn-type":[{"value":"0021-8561","type":"print"},{"value":"1520-5118","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026,9,3]]}}}