{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,19]],"date-time":"2026-08-19T15:25:07Z","timestamp":1787153107282,"version":"build-2736575974"},"reference-count":44,"publisher":"American Chemical Society (ACS)","issue":"32","license":[{"start":{"date-parts":[[2026,8,10]],"date-time":"2026-08-10T00:00:00Z","timestamp":1786320000000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-029"},{"start":{"date-parts":[[2026,8,10]],"date-time":"2026-08-10T00:00:00Z","timestamp":1786320000000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-037"},{"start":{"date-parts":[[2026,8,10]],"date-time":"2026-08-10T00:00:00Z","timestamp":1786320000000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-045"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["U24A20473"],"award-info":[{"award-number":["U24A20473"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100003489","name":"Henan University of Technology","doi-asserted-by":"publisher","award":["2024TXTD08"],"award-info":[{"award-number":["2024TXTD08"]}],"id":[{"id":"10.13039\/501100003489","id-type":"DOI","asserted-by":"publisher"}]},{"name":"Henan Provincial Special Fund for the Construction of Modern Agricultural Industrial Technology System","award":["No. HARS-22-02-G5"],"award-info":[{"award-number":["No. HARS-22-02-G5"]}]},{"name":"Key Research and development projects in Henan Province","award":["231111113300"],"award-info":[{"award-number":["231111113300"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2026,8,19]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>Aflatoxin B1 is a potent Group I carcinogen that contaminates food and feed, necessitating efficient detoxification strategies. This study isolated a novel bacterial strain, Enterobacter sp. HNGD-822 from soil, which efficiently degrades AFB1. Genome analysis identified two novel multicopper oxidase (laccase) genes, EbMCO1 and EbMCO2. The recombinant enzymes were heterologously expressed, exhibiting optimal activity at 50 \u00b0C and pH\u202f7.0, with strict Cu2+ dependence. Within 12 h, EbMCO1 degraded over 99% of AFB1, while EbMCO2 also showed high activity under the same conditions. Both enzymes primarily converted AFB1 into AFQ1 and epi-AFQ1, products that exhibited significantly reduced hepatotoxicity, developmental toxicity, and oxidative stress in transgenic zebrafish. Applied to artificially contaminated peanut meal matrices, EbMCO1 and EbMCO2 achieved degradation efficiencies of 89.35% and 82.66%, respectively, without altering nutritional quality. This work presents novel laccases with promising potential for the biocontrol of AFB1 in the feed and food industries.<\/jats:p>","DOI":"10.1021\/acs.jafc.6c04929","type":"journal-article","created":{"date-parts":[[2026,8,11]],"date-time":"2026-08-11T00:02:00Z","timestamp":1786406520000},"page":"25629-25643","source":"Crossref","is-referenced-by-count":0,"title":["Genome-Guided Discovery\nof\n                    <i>Enterobacter<\/i>\n                    Multicopper Oxidases Enabling Enzymatic\nDetoxification of Aflatoxin\nB1"],"prefix":"10.1021","volume":"74","author":[{"given":"Yan","family":"Zhang","sequence":"first","affiliation":[{"name":"Henan University of Technology , , ,","place":["Zhengzhou, P. R. China, 450001"]},{"name":"Henan Key Laboratory of Cereal and Oil Food Safety and Nutrition , ,","place":["Zhengzhou, P. R. 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China, 450001"]},{"name":"Henan Key Laboratory of Cereal and Oil Food Safety and Nutrition , ,","place":["Zhengzhou, P. R. China, 450001"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5096-8173","authenticated-orcid":true,"given":"Weibin","family":"Ma","sequence":"additional","affiliation":[{"name":"Henan University of Technology , , ,","place":["Zhengzhou, P. R. China, 450001"]},{"name":"Henan Key Laboratory of Cereal and Oil Food Safety and Nutrition , ,","place":["Zhengzhou, P. R. China, 450001"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yabo","family":"Liang","sequence":"additional","affiliation":[{"name":"Henan University of Technology , , ,","place":["Zhengzhou, P. R. China, 450001"]},{"name":"Henan Key Laboratory of Cereal and Oil Food Safety and Nutrition , ,","place":["Zhengzhou, P. R. China, 450001"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3895-5959","authenticated-orcid":true,"given":"Yanli","family":"Xie","sequence":"additional","affiliation":[{"name":"Henan University of Technology , , ,","place":["Zhengzhou, P. R. China, 450001"]},{"name":"Henan Key Laboratory of Cereal and Oil Food Safety and Nutrition , ,","place":["Zhengzhou, P. R. China, 450001"]}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"316","published-online":{"date-parts":[[2026,8,10]]},"reference":[{"key":"2026081910172069600_cit1","doi-asserted-by":"publisher","first-page":"S28","DOI":"10.1093\/toxsci\/kfq283","article-title":"Aflatoxin: A 50-Year\nOdyssey of Mechanistic and Translational Toxicology","volume":"120","author":"Kensler","year":"2011","journal-title":"Toxicol. Sci."},{"issue":"1\u20132","key":"2026081910172069600_cit2","doi-asserted-by":"publisher","first-page":"134","DOI":"10.1016\/j.anifeedsci.2011.12.014","article-title":"Mycotoxin\ncontamination of the feed supply chain: Implications for animal productivity\nand feed security","volume":"173","author":"Bryden","year":"2012","journal-title":"Anim. Feed Sci. Technol."},{"key":"2026081910172069600_cit3","doi-asserted-by":"publisher","first-page":"81","DOI":"10.1016\/j.fct.2018.11.047","article-title":"Aflatoxin B1: A review on metabolism, toxicity,\noccurrence in food, occupational exposure, and detoxification methods","volume":"124","author":"Rushing","year":"2019","journal-title":"Food Chem. Toxicol."},{"key":"2026081910172069600_cit4","doi-asserted-by":"publisher","DOI":"10.1038\/srep24328","article-title":"Aflatoxin B1 contamination in maize in Europe\nincreases due to climate change","volume":"6","author":"Battilani","year":"2016","journal-title":"Sci. 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Microbiol."},{"issue":"4","key":"2026081910172069600_cit31","doi-asserted-by":"publisher","first-page":"693","DOI":"10.1007\/s00449-025-03137-1","article-title":"Degradation and detoxification\nof aflatoxin B1 by two peroxidase enzymes from Irpex lacteus F17","volume":"48","author":"Xu","year":"2025","journal-title":"Bioprocess Biosyst. Eng."},{"key":"2026081910172069600_cit32","doi-asserted-by":"publisher","first-page":"13","DOI":"10.1016\/j.toxicon.2018.03.008","article-title":"Lactobacillus\nplantarum alleviate\naflatoxins (B1 and M1) induced disturbances\nin the intestinal genes expression and DNA fragmentation in mice","volume":"146","author":"Jebali","year":"2018","journal-title":"Toxicon"},{"issue":"36","key":"2026081910172069600_cit33","doi-asserted-by":"publisher","first-page":"22808","DOI":"10.1021\/acs.jafc.5c05089","article-title":"Mechanistic\nInsights\ninto Aflatoxin B1 and M1 Degradation by Bacillus subtilis HNGD-Mq02 from Wheat Koji and Its Application\nin Food Detoxification","volume":"73","author":"Ma","year":"2025","journal-title":"J. Agric. 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Toxicol."},{"issue":"5","key":"2026081910172069600_cit36","doi-asserted-by":"publisher","first-page":"869","DOI":"10.1007\/s00018-014-1826-6","article-title":"Electron transfer and reaction mechanism\nof laccases","volume":"72","author":"Jones","year":"2015","journal-title":"Cell. Mol. Life Sci."},{"issue":"2","key":"2026081910172069600_cit37","doi-asserted-by":"publisher","first-page":"53","DOI":"10.1007\/s11274-022-03491-2","article-title":"Biodegradation\nof ochratoxin A by\nendophytic Trichoderma koningii strains","volume":"39","author":"Ismaiel","year":"2023","journal-title":"World\nJ. Microbiol. 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Microbiol."},{"key":"2026081910172069600_cit40","doi-asserted-by":"publisher","DOI":"10.1016\/j.foodcont.2022.109472","article-title":"CotA laccase\nfrom Bacillus licheniformis ZOM-1 effectively degrades\nzearalenone, aflatoxin B1 and alternariol","volume":"145","author":"Sun","year":"2023","journal-title":"Food Control"},{"issue":"11","key":"2026081910172069600_cit41","doi-asserted-by":"publisher","first-page":"754","DOI":"10.3390\/toxins13110754","article-title":"Efficient degradation of aflatoxin\nB1 and zearalenone\nby laccase-like multicopper oxidase from Streptomyces thermocarboxydus\nin the presence of mediators","volume":"13","author":"Qin","year":"2021","journal-title":"Toxins"},{"key":"2026081910172069600_cit42","doi-asserted-by":"publisher","first-page":"147234","DOI":"10.1016\/j.foodchem.2025.147234","article-title":"In-depth study of zearalenone degradation\nby Ery 4 laccase-mediator\nsystems: in vitro efficacy, mechanistic insights,\nand application to contaminated corn","volume":"499","author":"Ciasca","year":"2026","journal-title":"Food Chem."},{"key":"2026081910172069600_cit43","doi-asserted-by":"publisher","first-page":"144035","DOI":"10.1016\/j.foodchem.2025.144035","article-title":"Comparison\nof the effects of three different fungal\nlaccases on the quality of rye bread","volume":"482","author":"Zhou","year":"2025","journal-title":"Food Chem."},{"issue":"4","key":"2026081910172069600_cit44","doi-asserted-by":"publisher","first-page":"298","DOI":"10.1515\/tjb-2015-0017","article-title":"Kinetics and\nmodelling of batch fermentation for the production of organic solvent\ntolerant and thermostable lipase by recombinant E. coli","volume":"40","author":"Bin Ariff","year":"2015","journal-title":"Turkish J. Biochem."}],"container-title":["Journal of Agricultural\nand Food Chemistry"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/pubs.acs.org\/doi\/pdf\/10.1021\/acs.jafc.6c04929","content-type":"application\/pdf","content-version":"vor","intended-application":"unspecified"},{"URL":"https:\/\/pubs.acs.org\/jafcau\/article-pdf\/74\/32\/25629\/66481732\/acs.jafc.6c04929.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"syndication"},{"URL":"https:\/\/pubs.acs.org\/jafcau\/article-pdf\/74\/32\/25629\/66481732\/acs.jafc.6c04929.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,8,19]],"date-time":"2026-08-19T14:31:43Z","timestamp":1787149903000},"score":1,"resource":{"primary":{"URL":"https:\/\/pubs.acs.org\/jafcau\/article\/74\/32\/25629\/5251742\/Genome-Guided-Discovery-of-Enterobacter"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,8,10]]},"references-count":44,"journal-issue":{"issue":"32","published-online":{"date-parts":[[2026,8,10]]},"published-print":{"date-parts":[[2026,8,19]]}},"URL":"https:\/\/doi.org\/10.1021\/acs.jafc.6c04929","relation":{},"ISSN":["0021-8561","1520-5118"],"issn-type":[{"value":"0021-8561","type":"print"},{"value":"1520-5118","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026,8,10]]}}}