{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,1]],"date-time":"2026-09-01T09:18:11Z","timestamp":1788254291347,"version":"build-2803163510"},"reference-count":40,"publisher":"American Chemical Society (ACS)","issue":"33","license":[{"start":{"date-parts":[[2026,8,17]],"date-time":"2026-08-17T00:00:00Z","timestamp":1786924800000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-029"},{"start":{"date-parts":[[2026,8,17]],"date-time":"2026-08-17T00:00:00Z","timestamp":1786924800000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-037"},{"start":{"date-parts":[[2026,8,17]],"date-time":"2026-08-17T00:00:00Z","timestamp":1786924800000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-045"}],"funder":[{"DOI":"10.13039\/501100007846","name":"Yunnan Provincial Department of Education","doi-asserted-by":"publisher","award":["2024J1110"],"award-info":[{"award-number":["2024J1110"]}],"id":[{"id":"10.13039\/501100007846","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100008871","name":"Yunnan Provincial Science and Technology Department","doi-asserted-by":"publisher","award":["202449CE340010"],"award-info":[{"award-number":["202449CE340010"]}],"id":[{"id":"10.13039\/501100008871","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/na","name":"the Basic Research Program of Yunnan Province","doi-asserted-by":"publisher","award":["202601AT070303"],"award-info":[{"award-number":["202601AT070303"]}],"id":[{"id":"10.13039\/na","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/na","name":"Yunnan Provincial Key Laboratory of Tea Science","doi-asserted-by":"publisher","award":["2026YNCX008"],"award-info":[{"award-number":["2026YNCX008"]}],"id":[{"id":"10.13039\/na","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/na","name":"the \"Xinglin Talent\" Program - Young Scientific and Technological Talent Special Project of Lincang City","doi-asserted-by":"publisher","award":["NA"],"award-info":[{"award-number":["NA"]}],"id":[{"id":"10.13039\/na","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/na","name":"Yunnan Science and Technology Vice President Project","doi-asserted-by":"publisher","award":["NA"],"award-info":[{"award-number":["NA"]}],"id":[{"id":"10.13039\/na","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2026,8,26]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>Strictinin, a bioactive hydrolyzable tannin in tea, varies widely among individual tea trees, but the basis of this variation remains unclear. Here, a rapid LC-MS\/MS method was established and applied to 157 individual large tea trees from Yunnan, revealing extensive natural variation in strictinin content. Comparative metabolomics of contrasting individuals showed that high ST accumulation was associated with altered partitioning of hydrolyzable tannin intermediates rather than increased gallic acid abundance alone, together with broader shifts in phenylpropanoid-related metabolism. Integration of metabolomics, transcriptomics, and weighted gene coexpression network analysis identified strictinin-associated modules and four oxidoreductase candidates, including CsLAC15, CsCYP81C13, CsCYP75B2, and CsCYP75A1. Transient antisense oligonucleotide silencing reduced strictinin accumulation and, for CsCYP81C13, increased gallic acid abundance. These results support an association between oxidoreductase-related metabolism and differential strictinin accumulation in individual large tea trees. These findings provide analytical and molecular resources for breeding tea varieties with elevated strictinin content.<\/jats:p>","DOI":"10.1021\/acs.jafc.6c04986","type":"journal-article","created":{"date-parts":[[2026,8,17]],"date-time":"2026-08-17T15:02:14Z","timestamp":1786978934000},"page":"26594-26609","source":"Crossref","is-referenced-by-count":0,"title":["Integrated Metabolomics,\nTranscriptomics, and Functional\nValidation Suggest Oxidoreductase-Associated Strictinin Accumulation\nin Individual Large Tea Trees from Yunnan"],"prefix":"10.1021","volume":"74","author":[{"ORCID":"https:\/\/orcid.org\/0009-0003-0528-5834","authenticated-orcid":true,"given":"Jin","family":"Sun","sequence":"first","affiliation":[{"name":"Southwest University , , No. 2 Tiansheng Road , Beibei District, ,","place":["Chongqing, China, 400715"]},{"name":"Southwest University , , No. 2 Tiansheng Road , Beibei District, ,","place":["Chongqing, China, 400715"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hongwei","family":"Dai","sequence":"additional","affiliation":[{"name":"Southwest University , , No. 2 Tiansheng Road , Beibei District, ,","place":["Chongqing, China, 400715"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0001-2445-7174","authenticated-orcid":true,"given":"Xin","family":"Zhang","sequence":"additional","affiliation":[{"name":"Southwest University , , No. 2 Tiansheng Road , Beibei District, ,","place":["Chongqing, China, 400715"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lianyu","family":"Yuan","sequence":"additional","affiliation":[{"name":"Southwest University , , No. 2 Tiansheng Road , Beibei District, ,","place":["Chongqing, China, 400715"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yiwei","family":"Weng","sequence":"additional","affiliation":[{"name":"Southwest University , , No. 2 Tiansheng Road , Beibei District, ,","place":["Chongqing, China, 400715"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Qiqian","family":"Su","sequence":"additional","affiliation":[{"name":"West Yunnan University , , ,","place":["Lincang, China, 677000"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xueyan","family":"Liu","sequence":"additional","affiliation":[{"name":"West Yunnan University , , ,","place":["Lincang, China, 677000"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ting","family":"Xu","sequence":"additional","affiliation":[{"name":"West Yunnan University , , ,","place":["Lincang, China, 677000"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yangtao","family":"Zhang","sequence":"additional","affiliation":[{"name":"Lincang Inspection Testing and Certification Institute , ,","place":["Lincang, China, 677000"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yanhong","family":"Liu","sequence":"additional","affiliation":[{"name":"West Yunnan University , , ,","place":["Lincang, China, 677000"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zhengfei","family":"Luo","sequence":"additional","affiliation":[{"name":"West Yunnan University , , ,","place":["Lincang, China, 677000"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4753-0055","authenticated-orcid":true,"given":"Huarong","family":"Tong","sequence":"additional","affiliation":[{"name":"Southwest University , , No. 2 Tiansheng Road , Beibei District, ,","place":["Chongqing, China, 400715"]}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"316","published-online":{"date-parts":[[2026,8,17]]},"reference":[{"key":"2026090104473082900_cit1","first-page":"25","article-title":"Bioactive polyphenols from foods and\ndietary supplements: Challenges and opportunities","volume-title":"Herbs: Challenges in Chemistry and Biology","author":"Seeram"},{"issue":"3","key":"2026090104473082900_cit2","doi-asserted-by":"publisher","first-page":"1080","DOI":"10.3390\/molecules28031080","article-title":"Strictinin, a Major Ingredient in\nYunnan Kucha Tea Possessing Inhibitory Activity on the Infection of\nMouse Hepatitis Virus to Mouse L Cells","volume":"28","author":"Tu","year":"2023","journal-title":"Molecules"},{"issue":"9","key":"2026090104473082900_cit3","doi-asserted-by":"publisher","first-page":"3961","DOI":"10.3390\/molecules28093961","article-title":"Strictinin:\nA Key Ingredient of Tea","volume":"28","author":"Tzen","year":"2023","journal-title":"Molecules"},{"key":"2026090104473082900_cit4","doi-asserted-by":"publisher","first-page":"116377","DOI":"10.1016\/j.jep.2023.116377","article-title":"The potential\nantidiabetic properties of green and purple tea Camellia sinensis (L.) 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Food Drug Anal"},{"key":"2026090104473082900_cit7","doi-asserted-by":"publisher","first-page":"113190","DOI":"10.1016\/j.jep.2020.113190","article-title":"Theacrine and\nstrictinin, two major ingredients for\nthe anti-influenza activity of Yunnan Kucha tea","volume":"262","author":"Lin","year":"2020","journal-title":"J. Ethnopharmacol"},{"key":"2026090104473082900_cit8","doi-asserted-by":"publisher","first-page":"72","DOI":"10.1016\/j.scienta.2011.10.017","article-title":"Light-induced\nexpression of genes involved in phenylpropanoid biosynthetic pathways\nin callus of tea (Camellia sinensis (L.) O. Kuntze)","volume":"133","author":"Wang","year":"2012","journal-title":"Sci. Hortic"},{"issue":"6","key":"2026090104473082900_cit9","doi-asserted-by":"publisher","first-page":"1139","DOI":"10.1007\/s00425-017-2760-2","article-title":"Transcriptional\nprofiling of catechins biosynthesis genes during tea plant leaf development","volume":"246","author":"Guo","year":"2017","journal-title":"Planta"},{"key":"2026090104473082900_cit10","doi-asserted-by":"publisher","first-page":"616","DOI":"10.1186\/s12864-018-4999-9","article-title":"Gene co-expression network\nanalysis reveals coordinated regulation\nof three characteristic secondary biosynthetic pathways in tea plant\n(Camellia sinensis)","volume":"19","author":"Tai","year":"2018","journal-title":"BMC Genomics"},{"issue":"3","key":"2026090104473082900_cit11","doi-asserted-by":"publisher","first-page":"241","DOI":"10.3390\/genes11030241","article-title":"Transcriptomic Analysis Reveals the\nMolecular Adaptation of Three Major Secondary Metabolic Pathways to\nMultiple Macronutrient Starvation in Tea (Camellia sinensis)","volume":"11","author":"Su","year":"2020","journal-title":"Genes"},{"issue":"12","key":"2026090104473082900_cit12","doi-asserted-by":"publisher","DOI":"10.1002\/fsn3.71277","article-title":"Biosynthesis and Application of Catechins\nand Their Derivatives in Camellia sinensis","volume":"13","author":"Yao","year":"2025","journal-title":"Food Sci. 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Yunnan."},{"issue":"4","key":"2026090104473082900_cit21","doi-asserted-by":"publisher","first-page":"402","DOI":"10.1006\/meth.2001.1262","article-title":"Analysis\nof relative gene expression data using real-time\nquantitative PCR and the 2(-Delta Delta C(T)) Method","volume":"25","author":"Livak","year":"2001","journal-title":"Methods"},{"issue":"4","key":"2026090104473082900_cit22","first-page":"1265","article-title":"Effect of microwave\nfixation on tea chemical components","volume":"6","author":"Cui-Cui","year":"2015","journal-title":"J. Food\nSaf. Food Qual."},{"key":"2026090104473082900_cit23","doi-asserted-by":"publisher","first-page":"102","DOI":"10.1007\/s11947-025-04203-y","article-title":"Enzymes Inactivation\nKinetics and Phytochemical Retention in Green Tea Leaves: A Comparative\nStudy of Microwave, Thermosonic, and Conventional Treatments","volume":"19","author":"Borah","year":"2026","journal-title":"Food Bioprocess Technol"},{"issue":"1","key":"2026090104473082900_cit24","doi-asserted-by":"publisher","first-page":"6656","DOI":"10.1038\/s41467-022-34537-6","article-title":"Metabolite\nannotation from knowns to unknowns through knowledge-guided multi-layer\nmetabolic networking","volume":"13","author":"Zhou","year":"2022","journal-title":"Nat. Commun"},{"issue":"7","key":"2026090104473082900_cit25","doi-asserted-by":"publisher","first-page":"621","DOI":"10.1038\/nmeth.1226","article-title":"Mapping and quantifying mammalian transcriptomes by RNA-Seq","volume":"5","author":"Mortazavi","year":"2008","journal-title":"Nat. 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Res."},{"issue":"13","key":"2026090104473082900_cit29","doi-asserted-by":"publisher","first-page":"4957","DOI":"10.1021\/jf070323f","article-title":"Simultaneous analysis of catechins,\ngallic acid, strictinin, and purine alkaloids in green tea by using\ncatechol as an internal standard","volume":"55","author":"Mizukami","year":"2007","journal-title":"J. Agric.\nFood Chem"},{"issue":"1","key":"2026090104473082900_cit30","doi-asserted-by":"publisher","first-page":"kiaf375","DOI":"10.1093\/plphys\/kiaf375","article-title":"Plant metabolism: Zoom in to the\nsingle-cell level","volume":"199","author":"Ntelkis","year":"2025","journal-title":"Plant Physiol"},{"issue":"3","key":"2026090104473082900_cit31","doi-asserted-by":"publisher","first-page":"301","DOI":"10.1016\/S0031-9422(02)00557-5","article-title":"Oxidation of pentagalloylglucose\nto the ellagitannin,\ntellimagrandin II, by a phenol oxidase from Tellima grandiflora leaves","volume":"62","author":"Niemetz","year":"2003","journal-title":"Phytochemistry"},{"issue":"17","key":"2026090104473082900_cit32","doi-asserted-by":"publisher","first-page":"2001","DOI":"10.1016\/j.phytochem.2005.01.009","article-title":"Enzymology of gallotannin\nand ellagitannin biosynthesis","volume":"66","author":"Niemetz","year":"2005","journal-title":"Phytochemistry"},{"key":"2026090104473082900_cit33","doi-asserted-by":"publisher","first-page":"754601","DOI":"10.3389\/fpls.2021.754601","article-title":"Phenoloxidases in Plants - How Structural Diversity\nEnables Functional Specificity","volume":"12","author":"Blaschek","year":"2021","journal-title":"Front. 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Plant\nSci"},{"key":"2026090104473082900_cit36","doi-asserted-by":"publisher","first-page":"144046","DOI":"10.1016\/j.gene.2019.144046","article-title":"Functional\nanalysis of flavonoid 3\u2032-hydroxylase\nand flavonoid 3\u2032,5\u2032-hydroxylases from tea plant (Camellia sinensis), involved in the B-ring hydroxylation\nof flavonoids","volume":"717","author":"Guo","year":"2019","journal-title":"Gene"},{"key":"2026090104473082900_cit37","doi-asserted-by":"publisher","first-page":"102575","DOI":"10.1016\/j.pbi.2024.102575","article-title":"Cell type-specific\ncontrol and post-translational\nregulation of specialized metabolism: opening new avenues for plant\nmetabolic engineering","volume":"81","author":"Ntelkis","year":"2024","journal-title":"Curr. Opin. Plant Biol"},{"issue":"1","key":"2026090104473082900_cit38","doi-asserted-by":"publisher","first-page":"135","DOI":"10.1007\/s11103-018-0808-8","article-title":"Post-translational\nand transcriptional regulation of phenylpropanoid biosynthesis pathway\nby Kelch repeat F-box protein SAGL1","volume":"99","author":"Yu","year":"2019","journal-title":"Plant Mol.\nBiol"},{"issue":"1","key":"2026090104473082900_cit39","doi-asserted-by":"publisher","first-page":"10","DOI":"10.1016\/j.antiviral.2010.06.008","article-title":"Antiviral effect of strictinin on\ninfluenza virus replication","volume":"88","author":"Saha","year":"2010","journal-title":"Antiviral Res"},{"issue":"2","key":"2026090104473082900_cit40","doi-asserted-by":"publisher","first-page":"86","DOI":"10.2174\/2772434417666220628153913","article-title":"Medicinal\nImportance, Pharmacological Activities, and Analytical Aspects of\nStrictinin: A Mini-Review","volume":"17","author":"Patel","year":"2022","journal-title":"Recent Adv. Anti-Infect.\nDrug Discov."}],"container-title":["Journal of Agricultural\nand Food Chemistry"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/pubs.acs.org\/doi\/pdf\/10.1021\/acs.jafc.6c04986","content-type":"application\/pdf","content-version":"vor","intended-application":"unspecified"},{"URL":"https:\/\/pubs.acs.org\/jafcau\/article-pdf\/74\/33\/26594\/66642799\/acs.jafc.6c04986.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"syndication"},{"URL":"https:\/\/pubs.acs.org\/jafcau\/article-pdf\/74\/33\/26594\/66642799\/acs.jafc.6c04986.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,9,1]],"date-time":"2026-09-01T08:47:38Z","timestamp":1788252458000},"score":1,"resource":{"primary":{"URL":"https:\/\/pubs.acs.org\/jafcau\/article\/74\/33\/26594\/5261635\/Integrated-Metabolomics-Transcriptomics-and"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,8,17]]},"references-count":40,"journal-issue":{"issue":"33","published-online":{"date-parts":[[2026,8,17]]},"published-print":{"date-parts":[[2026,8,26]]}},"URL":"https:\/\/doi.org\/10.1021\/acs.jafc.6c04986","relation":{},"ISSN":["0021-8561","1520-5118"],"issn-type":[{"value":"0021-8561","type":"print"},{"value":"1520-5118","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026,8,17]]}}}