{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,14]],"date-time":"2026-10-14T12:53:29Z","timestamp":1791982409089,"version":"4.4.0"},"reference-count":49,"publisher":"American Chemical Society (ACS)","issue":"40","license":[{"start":{"date-parts":[[2026,9,30]],"date-time":"2026-09-30T00:00:00Z","timestamp":1790726400000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-029"},{"start":{"date-parts":[[2026,9,30]],"date-time":"2026-09-30T00:00:00Z","timestamp":1790726400000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-037"},{"start":{"date-parts":[[2026,9,30]],"date-time":"2026-09-30T00:00:00Z","timestamp":1790726400000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-045"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["32402644"],"award-info":[{"award-number":["32402644"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/na","name":"General Program of Liaoning Provincial Science and Technology Joint Fund","doi-asserted-by":"publisher","award":["2024-MSLH-161"],"award-info":[{"award-number":["2024-MSLH-161"]}],"id":[{"id":"10.13039\/na","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/na","name":"Youth Project of the Department of Education of Liaoning Province","doi-asserted-by":"publisher","award":["jytqn2023439"],"award-info":[{"award-number":["jytqn2023439"]}],"id":[{"id":"10.13039\/na","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2026,10,14]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>Orange (Or) mutant cauliflower is prone to discoloration during storage, which affects its commercial value. \u03b3-Aminobutyric acid (GABA) treatment can effectively delay the discoloration of Or mutant cauliflower during storage; however, its mechanism of action remains unclear. Here, we aimed to identify key genes and candidate transcription factors associated with discoloration in Or cauliflower treated with GABA during storage. Transcriptome analysis revealed that GABA regulated BoLCYB, with BoMYB24 identified as the candidate transcription factor in this pathway. BoMYB24 binds the BoLCYB promoter through the MBS-1 motif. Additionally, under GABA treatment, the transcriptional activation effect of BoMYB24 on BoLCYB is further enhanced. BoMYB24 overexpression increases BoLCYB expression, leading to a large accumulation of \u03b2-carotene in chromoplasts and promoting the accumulation of auxin and abscisic acid. Furthermore, under GABA treatment, the transcription level of BoMYB24 was markedly upregulated, thus delaying the postharvest discoloration of Or mutant cauliflower by positively regulating BoLCYB.<\/jats:p>","DOI":"10.1021\/acs.jafc.6c05051","type":"journal-article","created":{"date-parts":[[2026,10,1]],"date-time":"2026-10-01T03:01:48Z","timestamp":1790823708000},"page":"31616-31629","source":"Crossref","is-referenced-by-count":0,"title":["\u03b3-Aminobutyric\nAcid Alleviates Discoloration\nof\n                    <i>Or<\/i>\n                    Mutant Cauliflower via BoMYB24-Mediated Transcriptional\nRegulation of\n                    <i>BoLCYB<\/i>"],"prefix":"10.1021","volume":"74","author":[{"given":"Wen-zheng","family":"Gao","sequence":"first","affiliation":[{"name":"Jinzhou Medical University , , ,","place":["Jinzhou, China, 121001"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0003-8905-950X","authenticated-orcid":true,"given":"Xi-zhu","family":"Wang","sequence":"additional","affiliation":[{"name":"Jinzhou Medical University , , ,","place":["Jinzhou, China, 121001"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2159-9377","authenticated-orcid":true,"given":"Yong-hong","family":"Ge","sequence":"additional","affiliation":[{"name":"Bohai University , , ,","place":["Jinzhou, China, 121013"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0008-0428-4078","authenticated-orcid":true,"given":"Feng","family":"Luo","sequence":"additional","affiliation":[{"name":"Jinzhou Medical University , , ,","place":["Jinzhou, China, 121001"]}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"316","published-online":{"date-parts":[[2026,9,30]]},"reference":[{"issue":"6","key":"2026101408091616000_cit1","doi-asserted-by":"publisher","first-page":"1235","DOI":"10.1038\/s41588-024-01744-4","article-title":"Genomic analyses reveal the stepwise domestication and genetic mechanism\nof curd biogenesis in cauliflower","volume":"56","author":"Chen","year":"2024","journal-title":"Nat. Genet."},{"issue":"4","key":"2026101408091616000_cit2","doi-asserted-by":"publisher","first-page":"588","DOI":"10.1139\/g03-043","article-title":"Molecular\nmapping of Or, a gene inducing\nbeta-carotene accumulation in cauliflower (Brassica oleracea L. var. botrytis)","volume":"46","author":"Li","year":"2003","journal-title":"Genome"},{"issue":"14","key":"2026101408091616000_cit3","doi-asserted-by":"publisher","DOI":"10.3390\/ijms25147603","article-title":"Bioactivity\nand bioavailability of carotenoids applied in human health: technological\nadvances and innovation","volume":"25","author":"Bas","year":"2024","journal-title":"Int. J. Mol. Sci."},{"issue":"8","key":"2026101408091616000_cit4","doi-asserted-by":"publisher","DOI":"10.3390\/antiox8080311","article-title":"Bioactive amines screening in four\ngenotypes of thermally processed cauliflower","volume":"8","author":"Diamante","year":"2019","journal-title":"Antioxidants"},{"issue":"9","key":"2026101408091616000_cit5","doi-asserted-by":"publisher","DOI":"10.3390\/plants10091939","article-title":"\u03b3-Aminobutyrate (GABA) Regulated\nplant defense: mechanisms and opportunities","volume":"10","author":"Shelp","year":"2021","journal-title":"Plants"},{"key":"2026101408091616000_cit6","doi-asserted-by":"publisher","DOI":"10.3389\/fpls.2022.884572","article-title":"\u03b3-Aminobutyrate improves the\npostharvest marketability of horticultural commodities: advances and\nprospects","volume":"13","author":"Aghdam","year":"2022","journal-title":"Front. Plant Sci."},{"key":"2026101408091616000_cit7","doi-asserted-by":"publisher","DOI":"10.1016\/j.foodchem.2025.144772","article-title":"\u03b3-aminobutyric\nacid: an emerging biomolecule for maintaining the postharvest quality\nof fresh fruits and vegetables: a meta-analysis","volume":"487","author":"Ali","year":"2025","journal-title":"Food Chem."},{"key":"2026101408091616000_cit8","doi-asserted-by":"publisher","DOI":"10.3389\/fnut.2021.752583","article-title":"Alleviation\nof postharvest chilling injury of carambola fruit by \u03b3-aminobutyric\nacid: physiological, biochemical, and structural characterization","volume":"8","author":"Mekontso","year":"2021","journal-title":"Front. Nutr."},{"key":"2026101408091616000_cit9","doi-asserted-by":"publisher","DOI":"10.1016\/j.ijbiomac.2023.123966","article-title":"The combined application of Arabic\ngum coating and \u03b3-aminobutyric acid mitigates chilling injury\nand maintains eating quality of \u2018Kinnow\u2019 mandarin fruits","volume":"236","author":"Ali","year":"2023","journal-title":"Int. J. Biol. Macromol."},{"issue":"13","key":"2026101408091616000_cit10","doi-asserted-by":"publisher","DOI":"10.3390\/ijms241310541","article-title":"Effects of L-cysteine and \u03b3-aminobutyric\nacid treatment on postharvest quality and antioxidant activity of\nloquat fruit during storage","volume":"24","author":"Zhang","year":"2023","journal-title":"Int. J. Mol. Sci."},{"issue":"16","key":"2026101408091616000_cit11","doi-asserted-by":"publisher","DOI":"10.3390\/foods13162485","article-title":"Innovative postharvest management for Hass avocado\nat the preclimacteric stage: A combined technology with GABA and 1-MCP","volume":"13","author":"Ruiz-Aracil","year":"2024","journal-title":"Foods"},{"issue":"B","key":"2026101408091616000_cit12","doi-asserted-by":"publisher","DOI":"10.1016\/j.foodchem.2022.134661","article-title":"\u03b3-aminobutyric acid treatment\ninduced chilling tolerance in postharvest kiwifruit (Actinidia chinensis cv. Hongyang) via regulating\nascorbic acid metabolism","volume":"404","author":"Liu","year":"2023","journal-title":"Food Chem."},{"issue":"6","key":"2026101408091616000_cit13","doi-asserted-by":"publisher","DOI":"10.1093\/h\/uhaf059","article-title":"Ethylene promotes anthocyanin\nsynthesis in \u2018Viviana\u2019 lily via the LvMYB5-LvERF113-LvMYB1\nmodule","volume":"12","author":"Zhang","year":"2025","journal-title":"Hortic. Res."},{"key":"2026101408091616000_cit14","doi-asserted-by":"publisher","first-page":"205","DOI":"10.1016\/j.jare.2022.02.005","article-title":"SmbHLH60 and SmMYC2 antagonistically regulate phenolic acids and\nanthocyanins biosynthesis in Salvia miltiorrhiza","volume":"42","author":"Liu","year":"2022","journal-title":"J. Adv. Res."},{"issue":"9","key":"2026101408091616000_cit15","doi-asserted-by":"publisher","DOI":"10.3390\/ijms25094841","article-title":"The transcription factor MiMYB8 suppresses peel coloration in postharvest\n\u2018Guifei\u2019 mango in response to high concentration of\nexogenous ethylene by negatively modulating MiPAL1","volume":"25","author":"Aslam","year":"2024","journal-title":"Int. J. Mol. Sci."},{"issue":"1","key":"2026101408091616000_cit16","doi-asserted-by":"publisher","DOI":"10.1186\/s12870-023-04049-6","article-title":"Betalain biosynthesis\nin red pulp\npitaya is regulated via HuMYB132: a R-R type MYB transcription factor","volume":"23","author":"Xie","year":"2023","journal-title":"BMC Plant Biol."},{"issue":"8","key":"2026101408091616000_cit17","doi-asserted-by":"publisher","DOI":"10.1016\/j.ijbiomac.2023.127665","article-title":"MiMYB10 transcription\nfactor regulates biosynthesis and accumulation of carotenoid involved\ngenes in mango fruit","volume":"253","author":"Dang","year":"2023","journal-title":"Int. J. Biol. Macromol."},{"issue":"41","key":"2026101408091616000_cit18","doi-asserted-by":"publisher","first-page":"12354","DOI":"10.1021\/acs.jafc.1c02703","article-title":"Integrative analyses of metabolomes and transcriptomes\nprovide insights into flavonoid variation in grape berries","volume":"69","author":"Lu","year":"2021","journal-title":"J. Agric. Food Chem."},{"issue":"12","key":"2026101408091616000_cit19","doi-asserted-by":"publisher","first-page":"4319","DOI":"10.1093\/jxb\/erab156","article-title":"Involvement of the\nR2R3-MYB transcription factor MYB21 and its homologs in regulating\nflavonol accumulation in Arabidopsis stamen","volume":"72","author":"Zhang","year":"2021","journal-title":"J. Exp. Bot."},{"issue":"1","key":"2026101408091616000_cit20","doi-asserted-by":"publisher","DOI":"10.1186\/s12864-022-08819-8","article-title":"Analysis of flavonol regulator evolution in the Brassicaceae\nreveals MYB12, MYB111 and MYB21 duplications and MYB11 and MYB24 gene\nloss","volume":"23","author":"Schilbert","year":"2022","journal-title":"BMC Genomics"},{"issue":"9","key":"2026101408091616000_cit21","doi-asserted-by":"publisher","DOI":"10.3390\/antiox13091140","article-title":"Exogenous ergothioneine\nand glutathione limit postharvest senescence\nof arugula","volume":"13","author":"Sivakumar","year":"2024","journal-title":"Antioxidants"},{"issue":"4","key":"2026101408091616000_cit22","doi-asserted-by":"publisher","DOI":"10.3390\/ijms23042186","article-title":"Transcriptome\nand metabolite conjoint analysis reveals the seed dormancy release\nprocess in Callery pear","volume":"23","author":"Zhang","year":"2022","journal-title":"Int. J. Mol. Sci."},{"issue":"6","key":"2026101408091616000_cit23","doi-asserted-by":"publisher","DOI":"10.1007\/s00425-022-04016-9","article-title":"Plastid diversity and chromoplast\nbiogenesis in differently coloured carrots: role of the DcOR3Leu gene","volume":"256","author":"Zhang","year":"2022","journal-title":"Planta"},{"issue":"12","key":"2026101408091616000_cit24","doi-asserted-by":"publisher","first-page":"3594","DOI":"10.1105\/tpc.106.046417","article-title":"The cauliflower or gene encodes\na DnaJ cysteine-rich domain-containing protein that mediates high\nlevels of beta-carotene accumulation","volume":"18","author":"Lu","year":"2007","journal-title":"Plant\nCell"},{"key":"2026101408091616000_cit25","doi-asserted-by":"publisher","DOI":"10.1016\/j.postharvbio.2022.112203","article-title":"Transcriptomic and metabolomic profiling reveal the\nrole of BoMYB2 in flavor regulation mechanism and coloration in the\npostharvest purple cauliflower","volume":"197","author":"Luo","year":"2023","journal-title":"Postharvest\nBiol. Technol."},{"issue":"4","key":"2026101408091616000_cit26","doi-asserted-by":"publisher","first-page":"1875","DOI":"10.1093\/plphys\/kiab013","article-title":"The mechanism for brassinosteroids\nsuppressing climacteric fruit ripening","volume":"185","author":"Ji","year":"2021","journal-title":"Plant\nPhysiol."},{"issue":"2","key":"2026101408091616000_cit27","doi-asserted-by":"publisher","first-page":"1312","DOI":"10.1093\/plphys\/kiab532","article-title":"F-box protein\nEBF1 and transcription factor ABI5-like regulate banana fruit chilling-induced\nripening disorder","volume":"188","author":"Song","year":"2022","journal-title":"Plant Physiol."},{"key":"2026101408091616000_cit28","doi-asserted-by":"publisher","DOI":"10.1038\/s41438-019-0155-1","article-title":"Transcriptome profiling reveals the\nroles of pigment mechanisms in postharvest broccoli yellowing","volume":"6","author":"Luo","year":"2019","journal-title":"Hortic. Res."},{"issue":"4","key":"2026101408091616000_cit29","doi-asserted-by":"publisher","first-page":"482","DOI":"10.1038\/nbt1082","article-title":"Improving the nutritional\nvalue of Golden Rice through increased pro-vitamin A content","volume":"23","author":"Paine","year":"2005","journal-title":"Nat. Biotechnol."},{"key":"2026101408091616000_cit30","doi-asserted-by":"publisher","DOI":"10.3389\/fpls.2019.01716","article-title":"Characterization\nof\ncauliflower or mutant variants","volume":"10","author":"Welsch","year":"2020","journal-title":"Front. Plant\nSci."},{"issue":"14","key":"2026101408091616000_cit31","doi-asserted-by":"publisher","first-page":"5535","DOI":"10.1073\/pnas.1115982109","article-title":"Carotenoid\noxidation\nproducts are stress signals that mediate gene responses to singlet\noxygen in plants","volume":"109","author":"Ramel","year":"2012","journal-title":"Proc. Natl. Acad. Sci. U.S.A."},{"issue":"1","key":"2026101408091616000_cit32","doi-asserted-by":"publisher","first-page":"643","DOI":"10.1093\/plphys\/kiad312","article-title":"Carotenoid\nsequestration protein FIBRILLIN participates in CmOR-regulated \u03b2-carotene\naccumulation in melon","volume":"193","author":"Zhou","year":"2023","journal-title":"Plant Physiol."},{"issue":"19","key":"2026101408091616000_cit33","doi-asserted-by":"publisher","DOI":"10.3390\/ijms251910749","article-title":"Relationship between the GABA pathway and signaling\nof other regulatory molecules","volume":"25","author":"Kaba\u0142a","year":"2024","journal-title":"Int. J. Mol.\nSci."},{"issue":"4","key":"2026101408091616000_cit34","doi-asserted-by":"publisher","first-page":"2005","DOI":"10.1093\/plphys\/kiab347","article-title":"The emerging role of GABA as a transport regulator\nand physiological signal","volume":"187","author":"Xu","year":"2021","journal-title":"Plant Physiol."},{"issue":"B","key":"2026101408091616000_cit35","doi-asserted-by":"publisher","DOI":"10.1016\/j.plaphy.2025.110436","article-title":"Ultrasound treatment\nretards senescence and promotes antioxidant capacity of fresh-cut\nbroccoli by modulating membrane lipid peroxidation, GABA and phenolics\naccumulation","volume":"229","author":"You","year":"2025","journal-title":"Plant Physiol. Biochem."},{"issue":"3","key":"2026101408091616000_cit36","doi-asserted-by":"publisher","DOI":"10.3390\/metabo13030347","article-title":"GABA metabolism, transport and their\nroles and mechanisms in the regulation of abiotic stress (hypoxia,\nsalt, drought) resistance in plants","volume":"13","author":"Yuan","year":"2023","journal-title":"Metabolites"},{"key":"2026101408091616000_cit37","doi-asserted-by":"publisher","DOI":"10.1016\/j.fochx.2024.101252","article-title":"Application\nof \u03b3-aminobutyric acid improves the postharvest marketability\nof strawberry by maintaining fruit quality and enhancing antioxidant\nsystem","volume":"21","author":"Zhang","year":"2024","journal-title":"Food Chem.: X"},{"key":"2026101408091616000_cit38","doi-asserted-by":"publisher","DOI":"10.1016\/j.fochx.2022.100208","article-title":"\u03b3-aminobutyric\nacid treatment reduces chilling injury and improves quality maintenance\nof cold-stored Chinese olive fruit","volume":"13","author":"Fan","year":"2022","journal-title":"Food Chem.:\nX"},{"issue":"1","key":"2026101408091616000_cit39","doi-asserted-by":"publisher","DOI":"10.3390\/ijms25010260","article-title":"Antioxidant systems and quality in\nsweet cherries are improved by preharvest GABA treatments leading\nto delay postharvest senescence","volume":"25","author":"Carrio\u0301n-Antoli\u0301","year":"2024","journal-title":"Int. J.Mol.\nSci."},{"issue":"1","key":"2026101408091616000_cit40","doi-asserted-by":"publisher","first-page":"87","DOI":"10.1104\/pp.19.00009","article-title":"A MYB triad controls primary and phenylpropanoid metabolites\nfor pollen coat patterning","volume":"180","author":"Battat","year":"2019","journal-title":"Plant Physiol."},{"issue":"6","key":"2026101408091616000_cit41","doi-asserted-by":"publisher","DOI":"10.3390\/molecules28062644","article-title":"MYB24 negatively regulates\nthe biosynthesis of lignin and capsaicin\nby affecting the expression of key genes in the phenylpropanoid metabolism\npathway in Capsicum chinense","volume":"28","author":"Yu","year":"2023","journal-title":"Molecules"},{"key":"2026101408091616000_cit42","doi-asserted-by":"publisher","DOI":"10.1016\/j.foodchem.2021.130076","article-title":"Transcriptome and metabolome\nprofiling\nto elucidate mechanisms underlying the blue discoloration of radish\nroots during storage","volume":"362","author":"Zhang","year":"2021","journal-title":"Food Chem."},{"issue":"12","key":"2026101408091616000_cit43","doi-asserted-by":"publisher","first-page":"4238","DOI":"10.1093\/plcell\/koad228","article-title":"MYB24\norchestrates terpene and flavonol metabolism as light responses\nto anthocyanin depletion in variegated grape berries","volume":"35","author":"Zhang","year":"2023","journal-title":"Plant Cell"},{"issue":"3","key":"2026101408091616000_cit44","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pgen.1000440","article-title":"Gibberellin\nacts through jasmonate to control the expression\nof MYB21, MYB24, and MYB57 to promote stamen filament growth in Arabidopsis","volume":"5","author":"Cheng","year":"2009","journal-title":"PLoS Genet."},{"issue":"3","key":"2026101408091616000_cit45","doi-asserted-by":"publisher","first-page":"559","DOI":"10.1105\/tpc.010410","article-title":"Expression\nprofile matrix of Arabidopsis transcription\nfactor genes suggests their putative functions in response to environmental\nstresses","volume":"14","author":"Chen","year":"2002","journal-title":"Plant Cell"},{"issue":"19","key":"2026101408091616000_cit46","doi-asserted-by":"publisher","first-page":"5865","DOI":"10.1093\/jxb\/eraa321","article-title":"N-glycosylation is\ninvolved in stomatal development by modulating\nthe release of active abscisic acid and auxin in Arabidopsis","volume":"71","author":"Jiao","year":"2020","journal-title":"J. Exp. Bot."},{"issue":"5","key":"2026101408091616000_cit47","doi-asserted-by":"publisher","first-page":"1696","DOI":"10.1111\/nph.18585","article-title":"Novel insights\ninto\nthe contribution of plastoglobules and reactive oxygen species to\nchromoplast differentiation","volume":"237","author":"Morelli","year":"2023","journal-title":"New Phytol."},{"key":"2026101408091616000_cit48","doi-asserted-by":"publisher","DOI":"10.3389\/fpls.2019.01250","article-title":"The role\nof carotenogenic metabolic flux in carotenoid\naccumulation and chromoplast differentiation: lessons from the melon\nfruit","volume":"10","author":"Feder","year":"2019","journal-title":"Front. Plant Sci."},{"issue":"1","key":"2026101408091616000_cit49","doi-asserted-by":"publisher","first-page":"295","DOI":"10.1007\/s00709-025-02106-5","article-title":"Transmission electron\nmicroscopy reveals the impact of Or gene on chromoplast\nmorphology and \u03b2-carotene accumulation\nin Or gene introgressed tropical cauliflower","volume":"263","author":"Chandel","year":"2026","journal-title":"Protoplasma"}],"container-title":["Journal of Agricultural\nand Food Chemistry"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/pubs.acs.org\/doi\/pdf\/10.1021\/acs.jafc.6c05051","content-type":"application\/pdf","content-version":"vor","intended-application":"unspecified"},{"URL":"https:\/\/pubs.acs.org\/jafcau\/article-pdf\/doi\/10.1021\/acs.jafc.6c05051\/68721172\/acs.jafc.6c05051.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"syndication"},{"URL":"https:\/\/pubs.acs.org\/jafcau\/article-pdf\/doi\/10.1021\/acs.jafc.6c05051\/68721172\/acs.jafc.6c05051.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,10,14]],"date-time":"2026-10-14T12:28:30Z","timestamp":1791980910000},"score":1,"resource":{"primary":{"URL":"https:\/\/pubs.acs.org\/jafcau\/article\/74\/40\/31616\/5443071\/Aminobutyric-Acid-Alleviates-Discoloration-of-Or"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,9,30]]},"references-count":49,"journal-issue":{"issue":"40","published-online":{"date-parts":[[2026,9,30]]},"published-print":{"date-parts":[[2026,10,14]]}},"URL":"https:\/\/doi.org\/10.1021\/acs.jafc.6c05051","relation":{},"ISSN":["0021-8561","1520-5118"],"issn-type":[{"value":"0021-8561","type":"print"},{"value":"1520-5118","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026,9,30]]}}}