{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,23]],"date-time":"2026-10-23T19:44:42Z","timestamp":1792784682172,"version":"4.5.0"},"reference-count":32,"publisher":"American Chemical Society (ACS)","issue":"37","license":[{"start":{"date-parts":[[2026,9,9]],"date-time":"2026-09-09T00:00:00Z","timestamp":1788912000000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-029"},{"start":{"date-parts":[[2026,9,9]],"date-time":"2026-09-09T00:00:00Z","timestamp":1788912000000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-037"},{"start":{"date-parts":[[2026,9,9]],"date-time":"2026-09-09T00:00:00Z","timestamp":1788912000000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-045"}],"funder":[{"name":"National Natural Science Foundation of China (NSFC)","award":["42306118"],"award-info":[{"award-number":["42306118"]}],"id":[{"id":"https:\/\/ror.org\/01h0zpd94","id-type":"ROR","asserted-by":"crossref"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2026,9,23]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>Chitin deacetylase is the key enzyme responsible for the biological deacetylation of chitin. However, its limited sources and low catalytic efficiency toward \u03b1-chitin present significant barriers to the bioproduction of chitosan. Herein, a chitin deacetylase Ce1425 sourced from Chitiniphilus eburneus was identified, which displayed optimal catalytic efficiency at pH 10.0 and 55 \u00b0C, maintaining 91.92% of its activity at pH 12.0. The presence of Co2+ enhanced its activity by 167.38%. Ce1425 exhibited the highest specific activity toward (GlcNAc)3 (8.45 U\/mg) and was capable of catalyzing the deacetylation of \u03b1-chitin. 12 h of catalysis reduced the acetylation degree of \u03b1-chitin by 24.65%. Ce1425 could remove up to three acetyl groups from chitooligosaccharide substrates, facilitated by the interactions between the substrates and Asp-83, His-137, Trp-203, and His-232. These findings broaden the sources of chitin deacetylases and provide valuable insights for the biopreparation of specifically deacetylated chitosan and its oligomers.<\/jats:p>","DOI":"10.1021\/acs.jafc.6c05212","type":"journal-article","created":{"date-parts":[[2026,9,9]],"date-time":"2026-09-09T09:02:12Z","timestamp":1788944532000},"page":"29407-29419","source":"Crossref","is-referenced-by-count":0,"title":["A Novel Alkaline-Stable\nChitin Deacetylase from\n                    <i>Chitiniphilus eburneus<\/i>\n                    for Eco-Friendly Preparation\nof Specific Deacetylated Products"],"prefix":"10.1021","volume":"74","author":[{"given":"Yuan","family":"Yu","sequence":"first","affiliation":[{"name":"Chinese Academy of Fishery Sciences , , ,","place":["Qingdao, China, 266071"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kailiang","family":"Leng","sequence":"additional","affiliation":[{"name":"Chinese Academy of Fishery Sciences , , ,","place":["Qingdao, China, 266071"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0946-930X","authenticated-orcid":true,"given":"Rong","family":"Cao","sequence":"additional","affiliation":[{"name":"Chinese Academy of Fishery Sciences , , ,","place":["Qingdao, China, 266071"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Junkui","family":"Miao","sequence":"additional","affiliation":[{"name":"Chinese Academy of Fishery Sciences , , ,","place":["Qingdao, China, 266071"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xixi","family":"Wang","sequence":"additional","affiliation":[{"name":"Chinese Academy of Fishery Sciences , , ,","place":["Qingdao, China, 266071"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yating","family":"Zhang","sequence":"additional","affiliation":[{"name":"Chinese Academy of Fishery Sciences , , ,","place":["Qingdao, China, 266071"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Dong","family":"Su","sequence":"additional","affiliation":[{"name":"Chinese Academy of Fishery Sciences , , ,","place":["Qingdao, China, 266071"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0002-4417-4152","authenticated-orcid":true,"given":"Xiaofang","family":"Liu","sequence":"additional","affiliation":[{"name":"Chinese Academy of Fishery Sciences , , ,","place":["Qingdao, China, 266071"]}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"316","published-online":{"date-parts":[[2026,9,9]]},"reference":[{"key":"2026092310085113900_cit1","doi-asserted-by":"publisher","first-page":"121123","DOI":"10.1016\/j.carbpol.2023.121123","article-title":"Engineering chitin deacetylase AsCDA for improving the catalytic efficiency towards crystalline\nchitin","volume":"318","author":"Yang","year":"2023","journal-title":"Carbohydr. 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