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A series of thiocarbamate derivatives were synthesized and evaluated against ten phytopathogenic fungi. Over 50% showed broad-spectrum fungicidal activity at 40 mg\/L. Notably, compound b6 exhibited superior activity against S. rolfsii (EC50 = 0.75 mg\/L), surpassing Thiram (EC50 = 0.91 mg\/L). In vivo assays confirmed robust protective and curative efficacies on peanut seedlings. b6 also demonstrated potent laccase inhibition (IC50 = 0.064 mmol\/L), significantly outperforming l-cysteine (IC50 = 0.551 mmol\/L) and PMDD-5Y (IC50 = 0.326 mmol\/L). Mechanistic studies revealed that b6 disrupts fungal cell membrane integrity and induces intracellular ROS accumulation. Overall, b6 represents a promising antifungal lead compound for designing novel laccase inhibitor-based fungicides.<\/jats:p>","DOI":"10.1021\/acs.jafc.6c05272","type":"journal-article","created":{"date-parts":[[2026,9,10]],"date-time":"2026-09-10T20:01:36Z","timestamp":1789070496000},"page":"29088-29099","source":"Crossref","is-referenced-by-count":0,"title":["Discovery of Thiram-Inspired\nThiocarbamate Derivatives\nas Novel Laccase Inhibitors against\n                    <i>Sclerotium rolfsii<\/i>"],"prefix":"10.1021","volume":"74","author":[{"given":"Rong","family":"Li","sequence":"first","affiliation":[{"name":"Anhui Agricultural University , , ,","place":["Hefei, P. R. China, 230036"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yuanjian","family":"Huang","sequence":"additional","affiliation":[{"name":"Anhui Agricultural University , , ,","place":["Hefei, P. R. 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