{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,1]],"date-time":"2026-07-01T16:49:13Z","timestamp":1782924553642,"version":"3.54.5"},"reference-count":35,"publisher":"American Chemical Society (ACS)","issue":"25","license":[{"start":{"date-parts":[[2026,6,17]],"date-time":"2026-06-17T00:00:00Z","timestamp":1781654400000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-029"},{"start":{"date-parts":[[2026,6,17]],"date-time":"2026-06-17T00:00:00Z","timestamp":1781654400000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-037"},{"start":{"date-parts":[[2026,6,17]],"date-time":"2026-06-17T00:00:00Z","timestamp":1781654400000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-045"}],"funder":[{"DOI":"10.13039\/501100002858","name":"China Postdoctoral Science Foundation","doi-asserted-by":"publisher","award":["2025M770911"],"award-info":[{"award-number":["2025M770911"]}],"id":[{"id":"10.13039\/501100002858","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["12375352"],"award-info":[{"award-number":["12375352"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100003995","name":"Natural Science Foundation of Anhui Province","doi-asserted-by":"publisher","award":["2308085MC76"],"award-info":[{"award-number":["2308085MC76"]}],"id":[{"id":"10.13039\/501100003995","id-type":"DOI","asserted-by":"publisher"}]},{"name":"YRD Collaborative Fundamental Research Project","award":["2024CSJZN0800"],"award-info":[{"award-number":["2024CSJZN0800"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["J. Agric. Food Chem."],"published-print":{"date-parts":[[2026,7,1]]},"DOI":"10.1021\/acs.jafc.6c05362","type":"journal-article","created":{"date-parts":[[2026,6,17]],"date-time":"2026-06-17T17:11:26Z","timestamp":1781716286000},"page":"19859-19870","source":"Crossref","is-referenced-by-count":0,"title":["5\u2032-End Translationalization: Iterative Assembly of Leaderless Polycistronic Amplifiers for Context-Independent Expression in the Food-Grade Bacterium\n                    <i>Corynebacterium glutamicum<\/i>"],"prefix":"10.1021","volume":"74","author":[{"given":"Manman","family":"Sun","sequence":"first","affiliation":[{"name":"Key Laboratory of High Magnetic Field and Ion Beam Physical Biology","place":["Hefei, China"]},{"name":"Hefei Institutes of Physical Science, Chinese Academy of Sciences","place":["Hefei, China"]},{"name":"Department of Bioengineering and Imperial College Centre for Synthetic Biology","place":["London, U.K."]},{"name":"Imperial College London","place":["London, U.K."]},{"name":"Institute of Hefei Artificial Intelligence Breeding Accelerator","place":["Hefei, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Bin","family":"Ye","sequence":"additional","affiliation":[{"name":"Key Laboratory of High Magnetic Field and Ion Beam Physical Biology","place":["Hefei, China"]},{"name":"Hefei Institutes of Physical Science, Chinese Academy of Sciences","place":["Hefei, China"]},{"name":"Institute of Hefei Artificial Intelligence Breeding Accelerator","place":["Hefei, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yimeng","family":"Zhao","sequence":"additional","affiliation":[{"name":"Key Laboratory of High Magnetic Field and Ion Beam Physical Biology","place":["Hefei, China"]},{"name":"Hefei Institutes of Physical Science, Chinese Academy of Sciences","place":["Hefei, China"]},{"name":"Institute of Hefei Artificial Intelligence Breeding Accelerator","place":["Hefei, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2631-5898","authenticated-orcid":true,"given":"Rodrigo","family":"Ledesma-Amaro","sequence":"additional","affiliation":[{"name":"Department of Bioengineering and Imperial College Centre for Synthetic Biology","place":["London, U.K."]},{"name":"Imperial College London","place":["London, U.K."]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Farkhod","family":"Eshboev","sequence":"additional","affiliation":[{"name":"Institute for Advanced Studies","place":["Tashkent, Uzbekistan"]},{"name":"New Uzbekistan University","place":["Tashkent, Uzbekistan"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Alex Xiong","family":"Gao","sequence":"additional","affiliation":[{"name":"Division of Life Science","place":["Kowloon, China"]},{"name":"The Hong Kong University of Science and Technology","place":["Kowloon, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Peng","family":"Wang","sequence":"additional","affiliation":[{"name":"Key Laboratory of High Magnetic Field and Ion Beam Physical Biology","place":["Hefei, China"]},{"name":"Hefei Institutes of Physical Science, Chinese Academy of Sciences","place":["Hefei, China"]},{"name":"Institute of Hefei Artificial Intelligence Breeding Accelerator","place":["Hefei, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"316","published-online":{"date-parts":[[2026,6,17]]},"reference":[{"key":"ref1\/cit1","doi-asserted-by":"publisher","DOI":"10.3109\/07388551.2015.1004519"},{"key":"ref2\/cit2","doi-asserted-by":"publisher","DOI":"10.1080\/07388551.2016.1206059"},{"key":"ref3\/cit3","doi-asserted-by":"publisher","DOI":"10.1016\/j.jbiotec.2017.02.023"},{"key":"ref4\/cit4","doi-asserted-by":"publisher","DOI":"10.3389\/fmicb.2018.02523"},{"key":"ref5\/cit5","doi-asserted-by":"publisher","DOI":"10.1038\/s41467-021-21740-0"},{"key":"ref6\/cit6","doi-asserted-by":"publisher","DOI":"10.1021\/acssynbio.1c00442"},{"key":"ref7\/cit7","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/gkaa786"},{"key":"ref8\/cit8","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/gkt151"},{"key":"ref9\/cit9","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/gkaa325"},{"key":"ref10\/cit10","doi-asserted-by":"publisher","DOI":"10.1007\/s00253-021-11611-5"},{"key":"ref11\/cit11","doi-asserted-by":"publisher","DOI":"10.1038\/nmeth.2404"},{"key":"ref12\/cit12","doi-asserted-by":"publisher","DOI":"10.1021\/acssynbio.0c00405"},{"key":"ref13\/cit13","doi-asserted-by":"publisher","DOI":"10.1021\/acssynbio.3c00246"},{"key":"ref14\/cit14","doi-asserted-by":"publisher","unstructured":"Beck, H. J.; Moll, I.Leaderless MRNAs in the Spotlight: Ancient but Not Outdated!\n                      Microbiol. Spectr.\n                      2018; Vol. 6 410.1128\/microbiolspec.RWR-0016-2017.","DOI":"10.1128\/microbiolspec.RWR-0016-2017"},{"key":"ref15\/cit15","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/gkac1039"},{"key":"ref16\/cit16","doi-asserted-by":"publisher","DOI":"10.1007\/s00253-022-11918-x"},{"key":"ref17\/cit17","doi-asserted-by":"publisher","DOI":"10.1016\/j.synbio.2025.08.001"},{"key":"ref18\/cit18","doi-asserted-by":"publisher","DOI":"10.1186\/s12934-020-01370-9"},{"key":"ref19\/cit19","doi-asserted-by":"publisher","DOI":"10.1093\/nargab\/lqab081"},{"key":"ref20\/cit20","doi-asserted-by":"publisher","DOI":"10.1021\/acssuschemeng.0c09341"},{"key":"ref21\/cit21","doi-asserted-by":"publisher","DOI":"10.1002\/1522-2683(200112)22:20<4404:AID-ELPS4404>3.0.CO;2-2"},{"key":"ref22\/cit22","doi-asserted-by":"publisher","DOI":"10.1007\/s10529-018-2539-y"},{"key":"ref23\/cit23","doi-asserted-by":"publisher","DOI":"10.1002\/bit.24954"},{"key":"ref24\/cit24","doi-asserted-by":"publisher","DOI":"10.1016\/j.tibtech.2024.01.003"},{"key":"ref25\/cit25","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/gkv635"},{"key":"ref26\/cit26","doi-asserted-by":"publisher","DOI":"10.1017\/S1466252307001338"},{"key":"ref27\/cit27","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.1222534110"},{"key":"ref28\/cit28","doi-asserted-by":"publisher","DOI":"10.1038\/s41587-019-0164-5.Human"},{"key":"ref29\/cit29","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pgen.1005641"},{"key":"ref30\/cit30","doi-asserted-by":"publisher","DOI":"10.1016\/S0022-2836(03)00809-X"},{"key":"ref31\/cit31","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/gkaf049"},{"key":"ref32\/cit32","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.1718029115"},{"key":"ref33\/cit33","doi-asserted-by":"publisher","DOI":"10.1038\/s41564-024-01775-x"},{"key":"ref34\/cit34","doi-asserted-by":"publisher","DOI":"10.1016\/j.molcel.2018.12.009"},{"key":"ref35\/cit35","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/gkaa304"}],"container-title":["Journal of Agricultural and Food Chemistry"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/pubs.acs.org\/doi\/pdf\/10.1021\/acs.jafc.6c05362","content-type":"application\/pdf","content-version":"vor","intended-application":"unspecified"},{"URL":"https:\/\/pubs.acs.org\/doi\/pdf\/10.1021\/acs.jafc.6c05362","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,7,1]],"date-time":"2026-07-01T15:56:07Z","timestamp":1782921367000},"score":1,"resource":{"primary":{"URL":"https:\/\/pubs.acs.org\/doi\/10.1021\/acs.jafc.6c05362"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,6,17]]},"references-count":35,"journal-issue":{"issue":"25","published-print":{"date-parts":[[2026,7,1]]}},"alternative-id":["10.1021\/acs.jafc.6c05362"],"URL":"https:\/\/doi.org\/10.1021\/acs.jafc.6c05362","relation":{},"ISSN":["0021-8561","1520-5118"],"issn-type":[{"value":"0021-8561","type":"print"},{"value":"1520-5118","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026,6,17]]}}}