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Here, we identified a novel temperature-responsive promoter, PycgM, which maintained strong transcriptional activity at 37\u201352 \u00b0C. In a promoter\u2013mCherry reporter system, PycgM exhibited 2287.3-fold higher activity than P2 at 52 \u00b0C, demonstrating excellent compatibility with a thermotolerant host. Truncation analysis identified a 150-bp core functional region responsible for optimal activity under induction and heat stress. When applied to drive glutamate decarboxylase expression at 50 \u00b0C, PycgM enabled \u03b3-aminobutyric acid production of 391.67 g\/L with a 98.69% conversion rate, representing a 275% increase over 37 \u00b0C fermentation. The whole-cell biocatalyst retained 86% activity after five reuse cycles, and SEM analysis indicated acceptable structural stability despite moderate morphological changes. These results demonstrate that PycgM is a robust, high-temperature genetic element for efficient enzyme and metabolite production in thermotolerant hosts.<\/jats:p>","DOI":"10.1021\/acs.jafc.6c05908","type":"journal-article","created":{"date-parts":[[2026,9,3]],"date-time":"2026-09-03T01:04:16Z","timestamp":1788397456000},"page":"27698-27710","source":"Crossref","is-referenced-by-count":0,"title":["Identification of a Novel Thermal Promoter and Its\nApplication in Glutamate Decarboxylase Protein Expression in\n                    <i>Bacillus licheniformis<\/i>"],"prefix":"10.1021","volume":"74","author":[{"given":"Ying","family":"Xu","sequence":"first","affiliation":[{"name":"Jiangnan University , , , ,","place":["Wuxi, Jiangsu, PR China, 214122"]},{"name":"Jiangnan University , , , ,","place":["Wuxi, Jiangsu, PR China, 214122"]},{"name":"Jiangnan University , , , ,","place":["Wuxi, Jiangsu, PR China, 214122"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Pengyang","family":"Liu","sequence":"additional","affiliation":[{"name":"Jiangnan University , , , ,","place":["Wuxi, Jiangsu, PR China, 214122"]},{"name":"Jiangnan University , , , ,","place":["Wuxi, Jiangsu, PR China, 214122"]},{"name":"Jiangnan University , , , ,","place":["Wuxi, Jiangsu, PR China, 214122"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Fengxu","family":"Xiao","sequence":"additional","affiliation":[{"name":"Jiangnan University , , , ,","place":["Wuxi, Jiangsu, PR China, 214122"]},{"name":"Jiangnan University , , , ,","place":["Wuxi, Jiangsu, PR China, 214122"]},{"name":"Jiangnan University , , , ,","place":["Wuxi, Jiangsu, PR China, 214122"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jiawei","family":"Lu","sequence":"additional","affiliation":[{"name":"Jiangnan University , , , ,","place":["Wuxi, Jiangsu, PR China, 214122"]},{"name":"Jiangnan University , , , ,","place":["Wuxi, Jiangsu, PR China, 214122"]},{"name":"Jiangnan University , , , ,","place":["Wuxi, Jiangsu, PR China, 214122"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yupeng","family":"Zhang","sequence":"additional","affiliation":[{"name":"Jiangnan University , , , ,","place":["Wuxi, Jiangsu, PR China, 214122"]},{"name":"Jiangnan University , , , ,","place":["Wuxi, Jiangsu, PR China, 214122"]},{"name":"Jiangnan University , , , ,","place":["Wuxi, Jiangsu, PR China, 214122"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Luyao","family":"Wang","sequence":"additional","affiliation":[{"name":"Jiangnan University , , , ,","place":["Wuxi, Jiangsu, PR China, 214122"]},{"name":"Jiangnan University , , , ,","place":["Wuxi, Jiangsu, PR China, 214122"]},{"name":"Jiangnan University , , , ,","place":["Wuxi, Jiangsu, PR China, 214122"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3396-4132","authenticated-orcid":true,"given":"Youran","family":"Li","sequence":"additional","affiliation":[{"name":"Jiangnan University , , , ,","place":["Wuxi, Jiangsu, PR China, 214122"]},{"name":"Jiangnan University , , , ,","place":["Wuxi, Jiangsu, PR China, 214122"]},{"name":"Jiangnan University , , , ,","place":["Wuxi, Jiangsu, PR China, 214122"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5147-0238","authenticated-orcid":true,"given":"Guiyang","family":"Shi","sequence":"additional","affiliation":[{"name":"Jiangnan University , , , ,","place":["Wuxi, Jiangsu, PR China, 214122"]},{"name":"Jiangnan University , , , ,","place":["Wuxi, Jiangsu, PR China, 214122"]},{"name":"Jiangnan University , , , ,","place":["Wuxi, Jiangsu, PR China, 214122"]}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"316","published-online":{"date-parts":[[2026,8,31]]},"reference":[{"key":"2026090917073939600_cit1","doi-asserted-by":"publisher","first-page":"94","DOI":"10.1016\/j.copbio.2017.11.016","article-title":"Next generation\nindustrial biotechnology based on extremophilic\nbacteria","volume":"50","author":"Chen","year":"2018","journal-title":"Curr. 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