{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,14]],"date-time":"2026-10-14T12:53:54Z","timestamp":1791982434342,"version":"4.4.0"},"reference-count":44,"publisher":"American Chemical Society (ACS)","issue":"40","license":[{"start":{"date-parts":[[2026,10,1]],"date-time":"2026-10-01T00:00:00Z","timestamp":1790812800000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-029"},{"start":{"date-parts":[[2026,10,1]],"date-time":"2026-10-01T00:00:00Z","timestamp":1790812800000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-037"},{"start":{"date-parts":[[2026,10,1]],"date-time":"2026-10-01T00:00:00Z","timestamp":1790812800000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-045"}],"funder":[{"DOI":"10.13039\/501100012226","name":"Fundamental Research Funds for the Central Universities","doi-asserted-by":"publisher","award":["SCU2025D003"],"award-info":[{"award-number":["SCU2025D003"]}],"id":[{"id":"10.13039\/501100012226","id-type":"DOI","asserted-by":"publisher"}]},{"name":"National Basic Research Program of China (973 Program)","award":["2025YFF1000600"],"award-info":[{"award-number":["2025YFF1000600"]}]},{"name":"Department of Science and Technology of Sichuan Province","award":["2022ZDZX0015"],"award-info":[{"award-number":["2022ZDZX0015"]}]},{"name":"Department of Science and Technology of Sichuan Province","award":["2025ZHCG0014"],"award-info":[{"award-number":["2025ZHCG0014"]}]},{"name":"Department of Science and Technology of Sichuan Province","award":["SCCXTD-2024-3"],"award-info":[{"award-number":["SCCXTD-2024-3"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2026,10,14]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>Oil quality improvement is a primary target for rapeseed breeding, with particular emphasis on erucic acid. Although some QTLs for fatty acid metabolism have been identified, the genetic network of erucic acid remains poorly explored, except for FAE1. The present study constructed a high-density genetic map using an F2 population of 200 individuals from a cross between high-erucic and high-oleic parents and identified a novel QTL (HO\/HE17) for erucic acid content, which was validated in the F2:3 population. Fine mapping refined HO\/HE17 to a 552 kb region flanked by markers 17SNP2 and loc9 on chromosome C07, encompassing 17 annotated genes, with functional annotation and experimental validation highlighting BnaC07.KCS9 as the most promising candidate gene. Meanwhile, we identified the transcription factor BnaA06.RGA, which positively regulates BnaC07.KCS9. Therefore, this study proposed an RGA-KCS9 regulatory module for erucic acid, providing a new insight into the genetic improvement of very-long-chain fatty acid metabolism and valuable germplasm for developing ultra-high erucic acid varieties.<\/jats:p>","DOI":"10.1021\/acs.jafc.6c05947","type":"journal-article","created":{"date-parts":[[2026,10,1]],"date-time":"2026-10-01T21:01:22Z","timestamp":1790888482000},"page":"31361-31373","source":"Crossref","is-referenced-by-count":0,"title":["<i>BnaC07.KCS9<\/i>\n                    , a New Gene Regulating\nErucic Acid Metabolism in Rapeseed"],"prefix":"10.1021","volume":"74","author":[{"given":"Meng","family":"Yin","sequence":"first","affiliation":[{"name":"Sichuan University , , ,","place":["Chengdu, China, 610065"]},{"name":"Science and Technology Innovation Center of Sichuan Modern Seed Industry Group , ,","place":["Chengdu, China, 611100"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xuefei","family":"Jiang","sequence":"additional","affiliation":[{"name":"Science and Technology Innovation Center of Sichuan Modern Seed Industry Group , ,","place":["Chengdu, China, 611100"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lei","family":"Ding","sequence":"additional","affiliation":[{"name":"Mianyang Academy of Agricultural Sciences\/Crop Germplasm Innovation and Genetic Improvement Key Laboratory of Sichuan Province\/High Erucic Acid Rapeseed Engineering Technology Research Center of Mianyang City , ,","place":["Mianyang, China, 621023"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ruijia","family":"Zhu","sequence":"additional","affiliation":[{"name":"Chengdu No.7-Yingcai School , ,","place":["Chengdu, China, 610066"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tian","family":"Luo","sequence":"additional","affiliation":[{"name":"Sichuan University , , ,","place":["Chengdu, China, 610065"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shuang","family":"Lu","sequence":"additional","affiliation":[{"name":"Sichuan University , , ,","place":["Chengdu, China, 610065"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zihan","family":"Ni","sequence":"additional","affiliation":[{"name":"Sichuan University , , ,","place":["Chengdu, China, 610065"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mingting","family":"Wu","sequence":"additional","affiliation":[{"name":"Sichuan University , , ,","place":["Chengdu, China, 610065"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ziyue","family":"Xu","sequence":"additional","affiliation":[{"name":"Sichuan University , , ,","place":["Chengdu, China, 610065"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Maolin","family":"Wang","sequence":"additional","affiliation":[{"name":"Sichuan University , , ,","place":["Chengdu, China, 610065"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4101-9301","authenticated-orcid":true,"given":"Rui","family":"Wang","sequence":"additional","affiliation":[{"name":"Sichuan University , , ,","place":["Chengdu, China, 610065"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6357-9422","authenticated-orcid":true,"given":"Yun","family":"Zhao","sequence":"additional","affiliation":[{"name":"Sichuan University , , ,","place":["Chengdu, China, 610065"]}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"316","published-online":{"date-parts":[[2026,10,1]]},"reference":[{"key":"2026101408093073000_cit1","doi-asserted-by":"publisher","first-page":"209","DOI":"10.1007\/s11745-010-3524-y","article-title":"Dietary monounsaturated fatty acids\nare protective against metabolic syndrome and cardiovascular disease\nrisk factors","volume":"46","author":"Gillingham","year":"2011","journal-title":"Lipids"},{"key":"2026101408093073000_cit2","doi-asserted-by":"publisher","first-page":"335","DOI":"10.1038\/nrendo.2009.79","article-title":"Trans fatty\nacids: effects on metabolic syndrome, heart\ndisease and diabetes","volume":"5","author":"Micha","year":"2009","journal-title":"Nat. Rev. Endocrinol."},{"key":"2026101408093073000_cit3","doi-asserted-by":"publisher","first-page":"745","DOI":"10.1056\/NEJM198603203141204","article-title":"Comparison\nof monounsaturated fatty acids and carbohydrates for lowering plasma\ncholesterol","volume":"314","author":"Grundy","year":"1986","journal-title":"N. Engl. J. Med."},{"key":"2026101408093073000_cit4","doi-asserted-by":"publisher","first-page":"983","DOI":"10.1111\/pbi.13263","article-title":"The impact\nof reducing fatty acid\ndesaturation on the composition and thermal stability of rapeseed\noil","volume":"18","author":"Kaur","year":"2020","journal-title":"Plant Biotechnol. J."},{"key":"2026101408093073000_cit5","doi-asserted-by":"publisher","first-page":"540","DOI":"10.1016\/j.jnutbio.2004.04.001","article-title":"Decreased aortic early\natherosclerosis and associated\nrisk factors in hypercholesterolemic hamsters fed a high- or mid-oleic\nacid oil compared to a high-linoleic acid oil","volume":"15","author":"Nicolosi","year":"2004","journal-title":"J. Nutr. Biochem."},{"key":"2026101408093073000_cit6","doi-asserted-by":"publisher","first-page":"1299","DOI":"10.1111\/tpj.14102","article-title":"PlantFAdb:\na resource for exploring hundreds of plant fatty acid structures synthesized\nby thousands of plants and their phylogenetic relationships","volume":"96","author":"Ohlrogge","year":"2018","journal-title":"Plant J."},{"key":"2026101408093073000_cit7","doi-asserted-by":"publisher","first-page":"711","DOI":"10.1038\/s41477-018-0225-7","article-title":"Discontinuous\nfatty acid elongation yields hydroxylated seed oil with improved function","volume":"4","author":"Li","year":"2018","journal-title":"Nat. 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J."},{"key":"2026101408093073000_cit9","doi-asserted-by":"publisher","DOI":"10.3390\/nu13010247","article-title":"Long-chain\npolyunsaturated fatty\nacids (LCPUFAs) and the developing immune system: a narrative review","volume":"13","author":"Miles","year":"2021","journal-title":"Nutrients"},{"key":"2026101408093073000_cit10","doi-asserted-by":"publisher","first-page":"2399","DOI":"10.1007\/s00394-023-03160-6","article-title":"Maternal diet supplementation with high-docosahexaenoic-acid\ncanola oil, along with arachidonic acid, promotes immune system development\nin allergy-prone BALB\/c mouse offspring at 3 weeks of age","volume":"62","author":"Patel","year":"2023","journal-title":"Eur. J. Nutr."},{"key":"2026101408093073000_cit11","doi-asserted-by":"publisher","DOI":"10.1016\/j.foodchem.2024.139414","article-title":"Inclusion of oil\nfrom transgenic Camelina sativa in feed effectively\nsupplies EPA and DHA\nto Atlantic salmon (Salmo salar) grown to market\nsize in seawater pens","volume":"456","author":"Tocher","year":"2024","journal-title":"Food Chem."},{"key":"2026101408093073000_cit12","doi-asserted-by":"publisher","DOI":"10.3390\/molecules26030546","article-title":"Erucic acid-rich\nyellow mustard oil improves insulin resistance in KK-Ay Mice","volume":"26","author":"Takahashi","year":"2021","journal-title":"Molecules"},{"key":"2026101408093073000_cit13","doi-asserted-by":"publisher","first-page":"2643","DOI":"10.1007\/s11011-022-01022-4","article-title":"Could dietary\nerucic acid lower risk of brain tumors? An epidemiological look to\nChinese population with implications for prevention and treatment","volume":"37","author":"Altinoz","year":"2022","journal-title":"Metab. Brain Dis."},{"key":"2026101408093073000_cit14","doi-asserted-by":"publisher","first-page":"950","DOI":"10.1126\/science.1253435","article-title":"Plant genetics.\nEarly allopolyploid evolution in the post-Neolithic Brassica napus oilseed genome","volume":"345","author":"Chalhoub","year":"2014","journal-title":"Science"},{"key":"2026101408093073000_cit15","doi-asserted-by":"publisher","first-page":"715","DOI":"10.1007\/s00122-012-1863-1","article-title":"Identification\nof FAD2 and FAD3 genes in Brassica napus genome and\ndevelopment of allele-specific markers for high oleic and low linolenic\nacid contents","volume":"125","author":"Yang","year":"2012","journal-title":"Theor. Appl. Genet."},{"key":"2026101408093073000_cit16","doi-asserted-by":"publisher","first-page":"497","DOI":"10.1007\/s00122-006-0315-1","article-title":"Mapping of the loci\ncontrolling oleic and linolenic acid contents and development of fad2 and fad3 allele-specific markers in\ncanola (Brassica napus L.)","volume":"113","author":"Hu","year":"2006","journal-title":"Theor. Appl. Genet."},{"key":"2026101408093073000_cit17","doi-asserted-by":"publisher","DOI":"10.3389\/fpls.2017.01789","article-title":"The FAD2 gene in plants: occurrence,\nregulation, and role","volume":"8","author":"Dar","year":"2017","journal-title":"Front. Plant Sci."},{"key":"2026101408093073000_cit18","doi-asserted-by":"publisher","first-page":"1444","DOI":"10.1111\/pbi.13876","article-title":"Enhancing\nthe accumulation of eicosapentaenoic acid and docosahexaenoic acid\nin transgenic Camelina through the CRISPR-Cas9 inactivation of the\ncompeting FAE1 pathway","volume":"20","author":"Han","year":"2022","journal-title":"Plant\nBiotechnol. J."},{"key":"2026101408093073000_cit19","doi-asserted-by":"publisher","first-page":"2401","DOI":"10.1007\/s00122-020-03607-y","article-title":"Modifications of fatty acid profile through targeted\nmutation at BnaFAD2 gene with CRISPR\/Cas9-mediated\ngene editing in Brassica napus","volume":"133","author":"Huang","year":"2020","journal-title":"Theor. Appl. Genet."},{"key":"2026101408093073000_cit20","doi-asserted-by":"publisher","DOI":"10.3389\/fgene.2018.00399","article-title":"Identification and\nfunctional analysis of two new mutant BnFAD2 alleles\nthat confer elevated oleic acid content in\nrapeseed","volume":"9","author":"Long","year":"2018","journal-title":"Front. Genet."},{"key":"2026101408093073000_cit21","doi-asserted-by":"publisher","first-page":"63","DOI":"10.1016\/j.plaphy.2018.04.025","article-title":"CRISPR\/Cas9-mediated\ngenome editing\nof the fatty acid desaturase 2 gene in Brassica napus","volume":"131","author":"Okuzaki","year":"2018","journal-title":"Plant Physiol. Biochem."},{"key":"2026101408093073000_cit22","doi-asserted-by":"publisher","first-page":"2313","DOI":"10.1111\/pbi.13142","article-title":"A novel quantitative trait locus\non chromosome A9 controlling oleic acid content in Brassica napus","volume":"17","author":"Zhao","year":"2019","journal-title":"Plant Biotechnol. J."},{"key":"2026101408093073000_cit23","doi-asserted-by":"publisher","first-page":"512","DOI":"10.1007\/BF00417942","article-title":"Specific molecular\nmarker of the genes controlling linolenic acid content in rapeseed","volume":"93","author":"Jourdren","year":"1996","journal-title":"Theor. Appl. Genet."},{"key":"2026101408093073000_cit24","doi-asserted-by":"publisher","DOI":"10.3389\/fpls.2022.899076","article-title":"A review of\nerucic acid production in Brassicaceae oilseeds: progress and prospects\nfor the genetic engineering of high and low-erucic acid rapeseeds\n(Brassica napus)","volume":"13","author":"Wang","year":"2022","journal-title":"Front. Plant\nSci."},{"key":"2026101408093073000_cit25","doi-asserted-by":"publisher","first-page":"852","DOI":"10.1007\/s001220050812","article-title":"The two genes homologous\nto Arabidopsis FAE1 co-segregate with the two loci\ngoverning erucic acid\ncontent in Brassica napus","volume":"96","author":"Fourmann","year":"1998","journal-title":"Theor. Appl. Genet."},{"key":"2026101408093073000_cit26","doi-asserted-by":"publisher","first-page":"1472","DOI":"10.1111\/pbi.13314","article-title":"Dissection of genetic\narchitecture for glucosinolate accumulations in leaves and seeds of Brassica napus by genome-wide association study","volume":"18","author":"Liu","year":"2020","journal-title":"Plant Biotechnol. J."},{"key":"2026101408093073000_cit27","doi-asserted-by":"publisher","first-page":"470","DOI":"10.1016\/j.molp.2020.12.003","article-title":"Genome- and transcriptome-wide association\nstudies provide insights into the genetic basis of natural variation\nof seed oil content in Brassica napus","volume":"14","author":"Tang","year":"2021","journal-title":"Mol. Plant"},{"key":"2026101408093073000_cit28","doi-asserted-by":"publisher","DOI":"10.1186\/s12870-020-02774-w","article-title":"Genome-wide\nassociation\nstudy reveals a patatin-like lipase relating to the reduction of seed\noil content in Brassica napus","volume":"21","author":"Wang","year":"2021","journal-title":"BMC Plant Biol."},{"key":"2026101408093073000_cit29","doi-asserted-by":"crossref","first-page":"506","DOI":"10.1007\/978-94-015-8394-7_139","article-title":"Reducing Polyunsaturation\nin Oils of Transgenic Canola and Soybean","volume-title":"Plant Lipid Metabolism","author":"Hitz","year":"1995"},{"key":"2026101408093073000_cit30","doi-asserted-by":"publisher","first-page":"1973","DOI":"10.2135\/cropsci2010.07.0411","article-title":"Influence of oleic\nacid content on yield in winter\noilseed rape","volume":"51","author":"Schierholt","year":"2011","journal-title":"Crop Sci."},{"key":"2026101408093073000_cit31","doi-asserted-by":"publisher","first-page":"187","DOI":"10.1007\/s00122-005-0124-y","article-title":"SMOOTH: a statistical\nmethod for successful removal of genotyping errors from high-density\ngenetic linkage data","volume":"112","author":"van Os","year":"2005","journal-title":"Theor. Appl. Genet."},{"key":"2026101408093073000_cit32","doi-asserted-by":"publisher","first-page":"193","DOI":"10.1111\/pbi.12128","article-title":"Bottlenecks\nin erucic acid accumulation in genetically engineered ultrahigh erucic\nacid Crambe abyssinica","volume":"12","author":"Guan","year":"2014","journal-title":"Plant\nBiotechnol. J."},{"key":"2026101408093073000_cit33","doi-asserted-by":"publisher","first-page":"1389","DOI":"10.1104\/pp.109.4.1389","article-title":"Lysophosphatidic\nacid acyltransferase from meadowfoam mediates insertion of erucic\nacid at the sn-2 position of triacylglycerol in transgenic\nrapeseed oil","volume":"109","author":"Lassner","year":"1995","journal-title":"Plant Physiol."},{"key":"2026101408093073000_cit34","doi-asserted-by":"publisher","first-page":"105","DOI":"10.1007\/978-1-4614-0827-7_4","article-title":"Breeding Oil Quality\nfor Edible and Industrial Applications in Oilseed Rape and Linseed","volume-title":"Technological Innovations in Major World Oil Crops","author":"McVetty","year":"2012"},{"key":"2026101408093073000_cit35","doi-asserted-by":"publisher","first-page":"765","DOI":"10.1007\/s00122-008-0936-7","article-title":"Increasing\nerucic acid content through combination of endogenous low polyunsaturated\nfatty acids alleles with Ld-LPAAT + Bn-fae1 transgenes in rapeseed (Brassica napus L.)","volume":"118","author":"Nath","year":"2009","journal-title":"Theor. Appl. Genet."},{"key":"2026101408093073000_cit36","doi-asserted-by":"publisher","first-page":"79","DOI":"10.1023\/A:1006325431653","article-title":"Microspore embryogenesis\nof high sn-2 erucic acid Brassica oleracea germplasm","volume":"57","author":"Ferrie","year":"1999","journal-title":"Plant Cell, Tissue Organ Cult."},{"key":"2026101408093073000_cit37","doi-asserted-by":"publisher","first-page":"3027","DOI":"10.1007\/s00253-018-8859-y","article-title":"Biosynthesis of\nnervonic acid and perspectives for its production by microalgae and\nother microorganisms","volume":"102","author":"Fan","year":"2018","journal-title":"Appl. Microbiol. Biotechnol."},{"key":"2026101408093073000_cit38","doi-asserted-by":"publisher","DOI":"10.3389\/fpls.2021.738805","article-title":"Development\nand validation of kompetitive\nallele-specific PCR assays for erucic acid content in Indian mustard\n[Brassica juncea (L.) Czern and Coss.]","volume":"12","author":"Gill","year":"2021","journal-title":"Front. Plant Sci."},{"key":"2026101408093073000_cit39","doi-asserted-by":"publisher","first-page":"93","DOI":"10.1016\/j.plantsci.2013.05.008","article-title":"Extending the story of very-long-chain\nfatty acid elongation","volume":"210","author":"Haslam","year":"2013","journal-title":"Plant. Sci."},{"key":"2026101408093073000_cit40","doi-asserted-by":"publisher","first-page":"1041","DOI":"10.1093\/pcp\/pcz019","article-title":"Functional\noverlap of long-chain acyl-CoA synthetases in Arabidopsis","volume":"60","author":"Zhao","year":"2019","journal-title":"Plant Cell Physiol."},{"key":"2026101408093073000_cit41","doi-asserted-by":"publisher","first-page":"567","DOI":"10.1104\/pp.112.210450","article-title":"Arabidopsis\n3-ketoacyl-coenzyme a synthase9 is involved in the synthesis of tetracosanoic\nacids as precursors of cuticular waxes, suberins, sphingolipids, and\nphospholipids","volume":"162","author":"Kim","year":"2013","journal-title":"Plant Physiol."},{"key":"2026101408093073000_cit42","doi-asserted-by":"publisher","first-page":"3004","DOI":"10.1093\/jxb\/erab543","article-title":"Protein-protein\ninteractions in fatty acid elongase complexes are important for very-long-chain\nfatty acid synthesis","volume":"73","author":"Kim","year":"2022","journal-title":"J. Exp. Bot."},{"key":"2026101408093073000_cit43","doi-asserted-by":"publisher","first-page":"404","DOI":"10.1111\/plb.13364","article-title":"Expression and roles of GRAS gene\nfamily in plant growth, signal\ntransduction, biotic and abiotic stress resistance and symbiosis formation-a\nreview","volume":"24","author":"Khan","year":"2022","journal-title":"Plant Biol."},{"key":"2026101408093073000_cit44","doi-asserted-by":"publisher","first-page":"2011","DOI":"10.1111\/pbi.13628","article-title":"DELLA proteins BnaA6.RGA\nand BnaC7.RGA negatively regulate fatty\nacid biosynthesis by interacting with BnaLEC1s in Brassica napus","volume":"19","author":"Yan","year":"2021","journal-title":"Plant Biotechnol. J."}],"container-title":["Journal of Agricultural\nand Food Chemistry"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/pubs.acs.org\/doi\/pdf\/10.1021\/acs.jafc.6c05947","content-type":"application\/pdf","content-version":"vor","intended-application":"unspecified"},{"URL":"https:\/\/pubs.acs.org\/jafcau\/article-pdf\/74\/40\/31361\/68730366\/acs.jafc.6c05947.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"syndication"},{"URL":"https:\/\/pubs.acs.org\/jafcau\/article-pdf\/74\/40\/31361\/68730366\/acs.jafc.6c05947.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,10,14]],"date-time":"2026-10-14T12:29:58Z","timestamp":1791980998000},"score":1,"resource":{"primary":{"URL":"https:\/\/pubs.acs.org\/jafcau\/article\/74\/40\/31361\/5443588\/BnaC07-KCS9-a-New-Gene-Regulating-Erucic-Acid"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,10,1]]},"references-count":44,"journal-issue":{"issue":"40","published-online":{"date-parts":[[2026,10,1]]},"published-print":{"date-parts":[[2026,10,14]]}},"URL":"https:\/\/doi.org\/10.1021\/acs.jafc.6c05947","relation":{},"ISSN":["0021-8561","1520-5118"],"issn-type":[{"value":"0021-8561","type":"print"},{"value":"1520-5118","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026,10,1]]}}}