{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,5]],"date-time":"2026-08-05T15:07:56Z","timestamp":1785942476897,"version":"3.56.0"},"reference-count":39,"publisher":"American Chemical Society (ACS)","issue":"30","license":[{"start":{"date-parts":[[2026,7,24]],"date-time":"2026-07-24T00:00:00Z","timestamp":1784851200000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-029"},{"start":{"date-parts":[[2026,7,24]],"date-time":"2026-07-24T00:00:00Z","timestamp":1784851200000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-037"},{"start":{"date-parts":[[2026,7,24]],"date-time":"2026-07-24T00:00:00Z","timestamp":1784851200000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-045"}],"funder":[{"DOI":"10.13039\/501100007846","name":"Yunnan Provincial Department of Education","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100007846","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100008871","name":"Yunnan Provincial Science and Technology Department","doi-asserted-by":"publisher","award":["2022YKZY001"],"award-info":[{"award-number":["2022YKZY001"]}],"id":[{"id":"10.13039\/501100008871","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["22267001"],"award-info":[{"award-number":["22267001"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["32360107"],"award-info":[{"award-number":["32360107"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["82260835"],"award-info":[{"award-number":["82260835"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["82460693"],"award-info":[{"award-number":["82460693"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2026,8,5]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>Fifteen new metabolites were isolated from Clonostachys rosea, a biocontrol fungus derived from Taxus yunnanensis. Among these, clonostachydimers A and B (14\u201315) are unprecedented anthrone-furanone hybrid dimers with a unique 6\/6\/6\/6\/5 pentacyclic framework, presumably biosynthesized via a [4 + 2] cycloaddition reaction. Their structures and absolute configurations were established by spectroscopic analyses, X-ray crystallography, ECD calculations, Snatzke\u2019s method, and DP4+ analysis. Bioassays indicated that several metabolites exhibited significant antimicrobial activity against drug-resistant pathogens as well as cytotoxic activity. Compound 3 showed potent cytotoxicity against A549\/Taxol cells (IC50 = 0.542 \u03bcM), exceeding that of doxorubicin and paclitaxel, while also displaying activity against MRSA and Candida albicans. Molecular docking suggested potential dual targeting of Topoisomerase II and P-glycoprotein, supporting its activity against paclitaxel-resistant cells.<\/jats:p>","DOI":"10.1021\/acs.jafc.6c05971","type":"journal-article","created":{"date-parts":[[2026,7,27]],"date-time":"2026-07-27T05:00:35Z","timestamp":1785128435000},"page":"23576-23590","source":"Crossref","is-referenced-by-count":0,"title":["Novel Anthrone-Furanone Hybrids and Monomers with\nAntidrug-Resistant Activity from the Biocontrol Fungus\n                    <i>Clonostachys rosea<\/i>"],"prefix":"10.1021","volume":"74","author":[{"given":"Hai-Li","family":"Han","sequence":"first","affiliation":[{"name":"Yunnan University , , ,","place":["Kunming, People\u2019s Republic of China, 650500"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3259-5737","authenticated-orcid":true,"given":"Xue-Qiong","family":"Yang","sequence":"additional","affiliation":[{"name":"Yunnan University , , ,","place":["Kunming, People\u2019s Republic of China, 650500"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yun-Xia","family":"Xie","sequence":"additional","affiliation":[{"name":"Yunnan University , , ,","place":["Kunming, People\u2019s Republic of China, 650500"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Long-Yue","family":"Zhang","sequence":"additional","affiliation":[{"name":"Yunnan University , , ,","place":["Kunming, People\u2019s Republic of China, 650500"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yi-Cen","family":"Tu","sequence":"additional","affiliation":[{"name":"Yunnan University , , ,","place":["Kunming, People\u2019s Republic of China, 650500"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0970-4920","authenticated-orcid":true,"given":"Le","family":"Cai","sequence":"additional","affiliation":[{"name":"Yunnan University , , ,","place":["Kunming, People\u2019s Republic of China, 650500"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5509-4559","authenticated-orcid":true,"given":"Ya-Bin","family":"Yang","sequence":"additional","affiliation":[{"name":"Yunnan University , , ,","place":["Kunming, People\u2019s Republic of China, 650500"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7860-060X","authenticated-orcid":true,"given":"Zhong-Tao","family":"Ding","sequence":"additional","affiliation":[{"name":"Yunnan University , , ,","place":["Kunming, People\u2019s Republic of China, 650500"]},{"name":"Yunnan University of Chinese Medicine , , ,","place":["Kunming, People\u2019s Republic of China, 650500"]}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"316","published-online":{"date-parts":[[2026,7,24]]},"reference":[{"key":"2026080510091819800_cit1","doi-asserted-by":"publisher","first-page":"1","DOI":"10.1007\/s13225-010-0034-4","article-title":"Fungal endophytes from\nhigher plants: A prolific source of phytochemicals and other bioactive\nnatural products","volume":"41","author":"Aly","year":"2010","journal-title":"Fungal Divers"},{"key":"2026080510091819800_cit2","doi-asserted-by":"publisher","first-page":"770","DOI":"10.1021\/acs.jnatprod.9b01285","article-title":"Natural products\nas sources of new drugs over the nearly\nfour decades from 01\/1981 to 09\/2019","volume":"83","author":"Newman","year":"2020","journal-title":"J. Nat.\nProd."},{"key":"2026080510091819800_cit3","doi-asserted-by":"publisher","first-page":"2325","DOI":"10.1021\/ja00738a045","article-title":"Plant antitumor\nagents. VI. The isolation and structure of taxol, a novel antileukemic\nand antitumor agent from Taxus brevifolia","volume":"93","author":"Wani","year":"1971","journal-title":"J. Am. Chem. Soc."},{"key":"2026080510091819800_cit4","doi-asserted-by":"publisher","first-page":"214","DOI":"10.1126\/science.8097061","article-title":"Taxol and taxane production by Taxomyces andreanae, an endophytic fungus of Pacific yew","volume":"260","author":"Stierle","year":"1993","journal-title":"Science"},{"key":"2026080510091819800_cit5","doi-asserted-by":"publisher","first-page":"2298","DOI":"10.1021\/acs.jafc.6b00556","article-title":"Isolation\nof secondary metabolites from the soil-derived fungus Clonostachys rosea YRS-06, a biological control agent, and evaluation\nof antibacterial activity","volume":"64","author":"Zhai","year":"2016","journal-title":"J. Agric. Food Chem."},{"key":"2026080510091819800_cit6","doi-asserted-by":"publisher","first-page":"110547","DOI":"10.1016\/j.scienta.2021.110547","article-title":"Insights into the molecular basis of biocontrol of Botrytis cinere by Clonostachys rosea in tomato","volume":"291","author":"Meng","year":"2022","journal-title":"Sci. Hortic."},{"key":"2026080510091819800_cit7","doi-asserted-by":"publisher","first-page":"11056","DOI":"10.1021\/acs.jafc.3c00447","article-title":"Asperphenalenones isolated from the biocontrol agent Clonostachys rosea and their antimicrobial activities","volume":"71","author":"Wang","year":"2023","journal-title":"J. Agric. Food Chem."},{"key":"2026080510091819800_cit8","doi-asserted-by":"publisher","first-page":"229","DOI":"10.3390\/jof6040229","article-title":"Metabolites from Clonostachys fungi\nand their biological activities","volume":"6","author":"Han","year":"2020","journal-title":"J. Fungi."},{"key":"2026080510091819800_cit9","doi-asserted-by":"publisher","first-page":"167","DOI":"10.1038\/s41579-018-0121-1","article-title":"Fungal\nsecondary metabolism: Regulation, function and drug discovery","volume":"17","author":"Keller","year":"2019","journal-title":"Nat. Rev. Microbiol."},{"issue":"10325","key":"2026080510091819800_cit10","doi-asserted-by":"crossref","first-page":"629","DOI":"10.1016\/S0140-6736(21)02724-0","article-title":"Global burden of bacterial antimicrobial resistance\nin 2019: A systematic analysis","volume":"399","author":"Murray","year":"2022","journal-title":"The Lancet"},{"key":"2026080510091819800_cit11","doi-asserted-by":"publisher","first-page":"203","DOI":"10.1038\/s41579-018-0147-4","article-title":"Methicillin-resistant Staphylococcus aureus: An overview of basic and clinical\nresearch","volume":"17","author":"Turner","year":"2019","journal-title":"Nat. Rev. Microbiol."},{"key":"2026080510091819800_cit12","doi-asserted-by":"publisher","first-page":"557","DOI":"10.1038\/s41579-022-00720-1","article-title":"Tackling the emerging\nthreat of antifungal resistance to human health","volume":"20","author":"Fisher","year":"2022","journal-title":"Nat. Rev. Microbiol."},{"key":"2026080510091819800_cit13","doi-asserted-by":"publisher","first-page":"521","DOI":"10.3390\/antibiotics4040521","article-title":"Livestock-associated\nMRSA: The impact\non humans","volume":"4","author":"Cuny","year":"2015","journal-title":"Antibiotics"},{"issue":"1","key":"2026080510091819800_cit14","doi-asserted-by":"publisher","first-page":"639","DOI":"10.1080\/21691401.2020.1728287","article-title":"KANK1 regulates\npaclitaxel resistance in lung adenocarcinoma A549 cells","volume":"48","author":"Pu","year":"2020","journal-title":"Artif. Cells, Nanomed., Biotechnol."},{"key":"2026080510091819800_cit15","doi-asserted-by":"publisher","first-page":"9000","DOI":"10.1021\/acs.jafc.4c12292","article-title":"Novel polyketides\nwith agricultural bioactivity produced by Phomopsis sp. HDSJ-28, an endophytic fungus derived from Taxus yunnanensis","volume":"73","author":"Han","year":"2025","journal-title":"J. Agric. Food Chem."},{"key":"2026080510091819800_cit16","doi-asserted-by":"publisher","first-page":"321","DOI":"10.1039\/B305068H","article-title":"Enzymatic catalysis\nof the Diels-Alder reaction in\nthe biosynthesis of natural products","volume":"21","author":"Oikawa","year":"2004","journal-title":"Nat. Prod.\nRep."},{"key":"2026080510091819800_cit17","doi-asserted-by":"publisher","first-page":"2492","DOI":"10.1038\/s41467-024-46845-0","article-title":"The evolutionary\norigin of naturally occurring intermolecular Diels-Alderases from Morus alba","volume":"15","author":"Ding","year":"2024","journal-title":"Nat. Commun."},{"key":"2026080510091819800_cit18","doi-asserted-by":"publisher","first-page":"107516","DOI":"10.1016\/j.cclet.2022.05.030","article-title":"Vercytochalasins A and B: Two unprecedented\nbiosynthetically\nrelated cytochalasins from the deep-sea-sourced endozoic fungus Curvularia verruculosa","volume":"34","author":"Hu","year":"2023","journal-title":"Chin. Chem.\nLett."},{"key":"2026080510091819800_cit19","doi-asserted-by":"publisher","first-page":"12526","DOI":"10.1021\/acs.joc.5b02396","article-title":"Beyond\nDP4: An improved probability\nfor the stereochemical assignment of isomeric compounds using quantum\nchemical calculations of NMR shifts","volume":"80","author":"Grimblat","year":"2015","journal-title":"J. Org.\nChem."},{"key":"2026080510091819800_cit20","doi-asserted-by":"publisher","first-page":"2404","DOI":"10.1021\/acs.jafc.4c09030","article-title":"Metabolites, biotransformation, and\nplant-growth dual regulatory activity from Aspergillus nishimurae uncovered by the fermentation interaction with a host","volume":"73","author":"Chen","year":"2025","journal-title":"J. Agric. Food Chem."},{"issue":"W1","key":"2026080510091819800_cit21","doi-asserted-by":"publisher","first-page":"W159","DOI":"10.1093\/nar\/gkac394","article-title":"CB-Dock2: Improved protein\u2013ligand blind docking by integrating\ncavity detection, docking and homologous template fitting","volume":"50","author":"Liu","year":"2022","journal-title":"Nucleic Acids Res."},{"key":"2026080510091819800_cit22","doi-asserted-by":"publisher","first-page":"7301","DOI":"10.1021\/jf4026663","article-title":"Lentisone,\na new phytotoxic anthraquinone produced by Ascochyta lentis, the causal agent of Ascochyta blight in Lens culinaris","volume":"61","author":"Andolfi","year":"2013","journal-title":"J. Agric. Food Chem."},{"issue":"12","key":"2026080510091819800_cit23","doi-asserted-by":"publisher","first-page":"1151","DOI":"10.1080\/10286020.2018.1520222","article-title":"Bioactive\nnaphthoquinone and anthrone derivatives from endophytic Micromonospora sp. NEAU-gq13","volume":"21","author":"Li","year":"2019","journal-title":"J. Asian Nat. Prod. Res."},{"key":"2026080510091819800_cit24","doi-asserted-by":"publisher","first-page":"929","DOI":"10.1590\/S0103-50532006000500017","article-title":"Novel anthraquinone\nderivatives produced by Phoma sorghina, an endophyte\nfound in association with the\nmedicinal plant Tithonia diversifolia (Asteraceae)","volume":"17","author":"Borges","year":"2006","journal-title":"J. Braz. Chem. Soc."},{"issue":"14","key":"2026080510091819800_cit25","doi-asserted-by":"publisher","first-page":"4819","DOI":"10.1021\/jo010136v","article-title":"Determination of absolute configuration of acyclic\n1,2-diols with Mo 2 (OAc) 4. 1. Snatzke\u2019s method revisited","volume":"66","author":"Di Bari","year":"2001","journal-title":"J. Org. Chem."},{"key":"2026080510091819800_cit26","doi-asserted-by":"publisher","first-page":"2906","DOI":"10.1021\/jo062445x","article-title":"Practical method for\nthe absolute configuration assignment of tert\/tert 1,2-diols using\ntheir complexes with Mo2(OAc)4","volume":"72","author":"Go\u0301recki","year":"2007","journal-title":"J. Org. Chem."},{"key":"2026080510091819800_cit27","doi-asserted-by":"publisher","first-page":"4593","DOI":"10.1021\/acs.orglett.8b01880","article-title":"Variecolortins A-C, three pairs of spirocyclic\ndiketopiperazine enantiomers from the marine-derived fungus Eurotium sp. SCSIO F452","volume":"20","author":"Zhong","year":"2018","journal-title":"Org. Lett."},{"key":"2026080510091819800_cit28","doi-asserted-by":"publisher","first-page":"1600","DOI":"10.1016\/j.tetlet.2019.05.025","article-title":"(+)- and (\u2212)-Eurotone\nA: A pair of enantiomeric\npolyketide dimers from a marine-derived fungus Eurotium sp. SCSIO F452","volume":"60","author":"Zhong","year":"2019","journal-title":"Tetrahedron Lett."},{"key":"2026080510091819800_cit29","doi-asserted-by":"publisher","first-page":"252","DOI":"10.1016\/j.fitote.2013.11.012","article-title":"Three pairs\nof variecolortide enantiomers from Eurotium sp. with\ncaspase-3 inhibitory activity","volume":"92","author":"Chen","year":"2014","journal-title":"Fitoterapia"},{"key":"2026080510091819800_cit30","doi-asserted-by":"publisher","first-page":"1206","DOI":"10.1021\/np300074z","article-title":"Albopunctatone,\nan antiplasmodial anthrone-anthraquinone from the Australian ascidian Didemnum albopunctatum","volume":"75","author":"Carroll","year":"2012","journal-title":"J. Nat. Prod."},{"key":"2026080510091819800_cit31","doi-asserted-by":"publisher","first-page":"1084","DOI":"10.1002\/anie.200704625","article-title":"Total synthesis and structure assignment\nof the anthrone C-glycoside cassialoin","volume":"47","author":"Koyama","year":"2008","journal-title":"Angew.\nChem., Int. Ed."},{"key":"2026080510091819800_cit32","doi-asserted-by":"publisher","first-page":"111780","DOI":"10.1016\/j.cclet.2025.111780","article-title":"Anti-MRSA anthrone\u2013macrolide\nhybrids from the ascomycete fungus Neonectria sp","volume":"37","author":"Ren","year":"2026","journal-title":"Chin. Chem. Lett."},{"key":"2026080510091819800_cit33","doi-asserted-by":"publisher","first-page":"9933","DOI":"10.1021\/jo301592k","article-title":"Navigating the fungal polyketide chemical space: From genes to molecules","volume":"77","author":"Chooi","year":"2012","journal-title":"J. Org. Chem."},{"key":"2026080510091819800_cit34","doi-asserted-by":"publisher","first-page":"2965","DOI":"10.1021\/ja8088185","article-title":"A gene cluster containing two fungal polyketide synthases\nencodes the biosynthetic pathway for a polyketide, asperfuranone,\nin Aspergillus nidulans","volume":"131","author":"Chiang","year":"2009","journal-title":"J.\nAm. Chem. Soc."},{"key":"2026080510091819800_cit35","doi-asserted-by":"publisher","first-page":"338","DOI":"10.1038\/nrc2607","article-title":"Targeting\nDNA topoisomerase II in cancer chemotherapy","volume":"9","author":"Nitiss","year":"2009","journal-title":"Nat. Rev. Cancer"},{"key":"2026080510091819800_cit36","doi-asserted-by":"publisher","first-page":"452","DOI":"10.1038\/s41568-018-0005-8","article-title":"Revisiting the role of ABC transporters in multidrug-resistant\ncancer","volume":"18","author":"Robey","year":"2018","journal-title":"Nat. Rev. Cancer"},{"key":"2026080510091819800_cit37","doi-asserted-by":"publisher","first-page":"216","DOI":"10.1186\/s40168-025-02231-6","article-title":"One health,\none microbiome","volume":"13","author":"Muhummed","year":"2025","journal-title":"Microbiome"},{"key":"2026080510091819800_cit38","doi-asserted-by":"publisher","first-page":"739","DOI":"10.1126\/science.aap7999","article-title":"Worldwide emergence\nof resistance to antifungal drugs challenges human health and food\nsecurity","volume":"360","author":"Fisher","year":"2018","journal-title":"Science"},{"key":"2026080510091819800_cit39","doi-asserted-by":"publisher","first-page":"377","DOI":"10.1007\/s00284-015-0958-8","article-title":"Control of\nbovine mastitis: Old and recent therapeutic\napproaches","volume":"72","author":"Gomes","year":"2016","journal-title":"Curr. Microbiol."}],"container-title":["Journal of Agricultural and Food Chemistry"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/pubs.acs.org\/doi\/pdf\/10.1021\/acs.jafc.6c05971","content-type":"application\/pdf","content-version":"vor","intended-application":"unspecified"},{"URL":"https:\/\/pubs.acs.org\/jafcau\/article-pdf\/74\/30\/23576\/66245523\/acs.jafc.6c05971.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"syndication"},{"URL":"https:\/\/pubs.acs.org\/jafcau\/article-pdf\/74\/30\/23576\/66245523\/acs.jafc.6c05971.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,8,5]],"date-time":"2026-08-05T14:29:19Z","timestamp":1785940159000},"score":1,"resource":{"primary":{"URL":"https:\/\/pubs.acs.org\/jafcau\/article\/74\/30\/23576\/5231692\/Novel-Anthrone-Furanone-Hybrids-and-Monomers-with"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,7,24]]},"references-count":39,"journal-issue":{"issue":"30","published-online":{"date-parts":[[2026,7,24]]},"published-print":{"date-parts":[[2026,8,5]]}},"URL":"https:\/\/doi.org\/10.1021\/acs.jafc.6c05971","relation":{},"ISSN":["0021-8561","1520-5118"],"issn-type":[{"value":"0021-8561","type":"print"},{"value":"1520-5118","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026,7,24]]}}}