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This method integrates mechanical densification with catalytic oxidation. It reduces chemical dosage to an ultra-low 0.0289 g\/g biomass, far below that of conventional oxidative pretreatments. Multi-scale characterization revealed that this method effectively disrupted the lignin-carbohydrate matrix without severe delignification, significantly enhancing biomass porosity and enzymatic digestibility. Consequently, DLCA(ft) pretreated corn stover achieved exceptional enzymatic digestibility (&amp;gt;90% total sugar conversion) even at 35% (w\/w) solid loading. Crucially, the minimal chemical usage suppressed the formation of many inhibitory degradation products, enabling direct fermentation to a high ethanol titer of 81.8 g\/L without water washing or detoxification. DLCA(ft) pretreatment addressed critical barriers of high reagent cost and low working mass in traditional pretreatment systems, representing a significant step toward the industrial realization of lignocellulosic biorefinery.<\/jats:p>","DOI":"10.1021\/acs.jafc.6c06174","type":"journal-article","created":{"date-parts":[[2026,9,24]],"date-time":"2026-09-24T17:02:00Z","timestamp":1790269320000},"page":"30940-30953","source":"Crossref","is-referenced-by-count":0,"title":["Synergistic Integration\nof Mechanical Densification\nand Ultra-Low-Dosage Fenton Oxidation Enables Efficient Deconstruction\nof Corn Stover and High-Titer Ethanol Production"],"prefix":"10.1021","volume":"74","author":[{"given":"Xinchuan","family":"Yuan","sequence":"first","affiliation":[{"name":"Nanjing University of Science and Technology , , 200 Xiaolingwei Street , ,","place":["Nanjing, China, 210094"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shizhong","family":"Yang","sequence":"additional","affiliation":[{"name":"Nanjing University of Science and Technology , , 200 Xiaolingwei Street , ,","place":["Nanjing, China, 210094"]},{"name":"Suzhou Pomoea Biotechnology Co., Ltd. , 337 Lushan Road , ,","place":["Suzhou, China, 215129"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Guannan","family":"Shen","sequence":"additional","affiliation":[{"name":"Nanjing University of Science and Technology , , 200 Xiaolingwei Street , ,","place":["Nanjing, China, 210094"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yao","family":"Chen","sequence":"additional","affiliation":[{"name":"Nanjing University of Science and Technology , , 200 Xiaolingwei Street , ,","place":["Nanjing, China, 210094"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xingye","family":"Hu","sequence":"additional","affiliation":[{"name":"Nanjing University of Science and Technology , , 200 Xiaolingwei Street , ,","place":["Nanjing, China, 210094"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shaohua","family":"Cheng","sequence":"additional","affiliation":[{"name":"Nanjing University of Science and Technology , , 200 Xiaolingwei Street , ,","place":["Nanjing, China, 210094"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0094-3178","authenticated-orcid":true,"given":"Zhaoxian","family":"Xu","sequence":"additional","affiliation":[{"name":"Nanjing University of Science and Technology , , 200 Xiaolingwei Street , ,","place":["Nanjing, China, 210094"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9493-305X","authenticated-orcid":true,"given":"Mingjie","family":"Jin","sequence":"additional","affiliation":[{"name":"Nanjing University of Science and Technology , , 200 Xiaolingwei Street , ,","place":["Nanjing, China, 210094"]}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"316","published-online":{"date-parts":[[2026,9,24]]},"reference":[{"issue":"1","key":"2026100711064605700_cit1","doi-asserted-by":"publisher","first-page":"1244","DOI":"10.1038\/s41467-025-56472-y","article-title":"Advances in lignocellulosic\nfeedstocks for bioenergy and bioproducts","volume":"16","author":"Sulis","year":"2025","journal-title":"Nat.\nCommun."},{"issue":"23","key":"2026100711064605700_cit2","doi-asserted-by":"publisher","first-page":"6787","DOI":"10.1021\/acs.jafc.1c07104","article-title":"Novel Approaches in the Valorization\nof Agricultural Wastes and Their Applications","volume":"70","author":"Capanoglu","year":"2022","journal-title":"Journal of Agricultural and Food Chemistry"},{"key":"2026100711064605700_cit3","doi-asserted-by":"publisher","DOI":"10.1016\/j.biortech.2020.122848","article-title":"Lignocellulosic biomass: Acid and\nalkaline pretreatments and their effects on biomass recalcitrance\n\u2013 Conventional processing and recent advances","volume":"304","author":"Lorenci Woiciechowski","year":"2020","journal-title":"Bioresour. 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