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The molecular basis of this differential sensitivity remains unclear. Here, we investigated how site-specific variations in Fusarium solani myosin I (FsMyoI) affect PHA sensitivity and evaluated FsMyoI as a target for inhibitor discovery. Molecular dynamics simulations, site-directed mutagenesis, binding assays, and ATPase activity measurements revealed that FsMyoIK376M and FsMyoIT218S mutations reshaped the dynamics of key functional regions, influencing PHA binding and ATPase inhibition. These findings guided virtual screening and led to three hits with confirmed antifungal activity and target engagement. These results provide structural insight into Fusarium species-specific PHA sensitivity and highlight FsMyoI as a potential target for novel antifungal development.<\/jats:p>","DOI":"10.1021\/acs.jafc.6c06252","type":"journal-article","created":{"date-parts":[[2026,8,7]],"date-time":"2026-08-07T13:02:00Z","timestamp":1786107720000},"page":"25335-25346","source":"Crossref","is-referenced-by-count":0,"title":["T218 and K376 Control Phenamacril Sensitivity in\n                    <i>Fusarium solani<\/i>\n                    Myosin I and Enable Structure-Based\nAntifungal Discovery"],"prefix":"10.1021","volume":"74","author":[{"ORCID":"https:\/\/orcid.org\/0009-0001-8866-2847","authenticated-orcid":true,"given":"Wei","family":"Yu","sequence":"first","affiliation":[{"name":"Nanjing Agricultural University , , ,","place":["Nanjing, China, 210095"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiyuan","family":"Zhu","sequence":"additional","affiliation":[{"name":"Nanjing Agricultural University , , ,","place":["Nanjing, China, 210095"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chaoqi","family":"He","sequence":"additional","affiliation":[{"name":"Nanjing Agricultural University , , ,","place":["Nanjing, China, 210095"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yongheng","family":"Tang","sequence":"additional","affiliation":[{"name":"Nanjing Agricultural University , , ,","place":["Nanjing, China, 210095"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Fan","family":"Zhong","sequence":"additional","affiliation":[{"name":"Nanjing Agricultural University , , ,","place":["Nanjing, China, 210095"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Bocong","family":"Cai","sequence":"additional","affiliation":[{"name":"Nanjing Agricultural University , , ,","place":["Nanjing, China, 210095"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yaguang","family":"Zhang","sequence":"additional","affiliation":[{"name":"Nanjing Agricultural University , , ,","place":["Nanjing, China, 210095"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shuai","family":"Yang","sequence":"additional","affiliation":[{"name":"Nanjing Agricultural University , , ,","place":["Nanjing, China, 210095"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4632-4364","authenticated-orcid":true,"given":"Jingjing","family":"Guo","sequence":"additional","affiliation":[{"name":"Macao Polytechnic University , , ,","place":["Macao, China, 999078"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jiaxing","family":"Yan","sequence":"additional","affiliation":[{"name":"Nanjing Agricultural University , , ,","place":["Nanjing, China, 210095"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Feng","family":"Zhang","sequence":"additional","affiliation":[{"name":"Nanjing Agricultural University , , ,","place":["Nanjing, China, 210095"]},{"name":"Nanjing Agricultural University , , ,","place":["Sanya, China, 572025"]}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"316","published-online":{"date-parts":[[2026,8,7]]},"reference":[{"issue":"9","key":"2026081910161941400_cit1","doi-asserted-by":"publisher","first-page":"4186","DOI":"10.1002\/ps.8122","article-title":"Rhizospheric\nmicrobiota of suppressive soil protect plants against Fusarium solani infection","volume":"80","author":"Li","year":"2024","journal-title":"Pest Manage. 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Comput.-Aided Mol.\nDes."},{"issue":"5","key":"2026081910161941400_cit31","doi-asserted-by":"publisher","first-page":"4282","DOI":"10.1021\/acs.jafc.5c12378","article-title":"Antifungal\nactivity and resistance risk of cyclobutrifluram against Fusarium solani, the causal agent of peanut root\nrot","volume":"74","author":"Li","year":"2026","journal-title":"J. Agric. Food Chem."},{"key":"2026081910161941400_cit32","doi-asserted-by":"publisher","first-page":"283","DOI":"10.1107\/S0021889892009944","article-title":"PROCHECK: a program\nto check the stereochemical quality of protein structures","volume":"26","author":"Laskowski","year":"1993","journal-title":"J. Appl. 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U.S.A."},{"issue":"4","key":"2026081910161941400_cit35","doi-asserted-by":"publisher","first-page":"1328","DOI":"10.1074\/jbc.RA118.005408","article-title":"Phenamacril\nis a reversible and noncompetitive inhibitor of Fusarium class I myosin","volume":"294","author":"Wollenberg","year":"2019","journal-title":"J. Biol. Chem."},{"issue":"1","key":"2026081910161941400_cit36","doi-asserted-by":"publisher","DOI":"10.1038\/s41467-022-32110-9","article-title":"Multistep orthophosphate release tunes actomyosin energy\ntransduction","volume":"13","author":"Moretto","year":"2022","journal-title":"Nat. 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