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Lactococcus lactis, a common host for waste valorization, shows poor protein expression in conventional systems. We constructed T7RNAP-driven inducible and constitutive expression systems in L. lactis NZ9000. The two systems increased target gene transcription by 103-fold and 207-fold, and enzyme activity by 5.24-fold and 10.36-fold, respectively. A signal peptide enabled efficient extracellular secretion of recombinant proteins. The engineered strains synthesized levansucrase and levan, achieving yields of 36.27 g\/L and 27.53 g\/L in sucrose medium. When using food waste substrates, the levan titer reached 8.84 g\/L with molasses, and 3.2, 1.4, 0.5, 0.7, 0.5 g\/L with sugar cane bagasse and various fruit peels. This work provides a reliable strategy for strain engineering, pomace reuse and low-cost large-scale levan production.<\/jats:p>","DOI":"10.1021\/acs.jafc.6c06579","type":"journal-article","created":{"date-parts":[[2026,8,6]],"date-time":"2026-08-06T07:01:13Z","timestamp":1785999673000},"page":"25463-25475","source":"Crossref","is-referenced-by-count":2,"title":["Construction of\n                    <i>Lactococcus lactis<\/i>\n                    Expression System via Chromosomal\nIntegration of T7 RNA Polymerase\nfor Levan Production from Fruit Pomace"],"prefix":"10.1021","volume":"74","author":[{"given":"Kaige","family":"Peng","sequence":"first","affiliation":[{"name":"Hebei Agricultural University , , 289 Lingyusi Road , , ,","place":["Baoding, Hebei, China, 071000"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6543-0373","authenticated-orcid":true,"given":"Fangkun","family":"Zhao","sequence":"additional","affiliation":[{"name":"Hebei Agricultural University , , 289 Lingyusi Road , , ,","place":["Baoding, Hebei, China, 071000"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xuejiao","family":"Zhang","sequence":"additional","affiliation":[{"name":"Hebei Agricultural University , , 289 Lingyusi Road , , ,","place":["Baoding, Hebei, China, 071000"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yakun","family":"Hou","sequence":"additional","affiliation":[{"name":"University of Otago , , ,","place":["Dunedin, New Zealand, 9054"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kemin","family":"Mao","sequence":"additional","affiliation":[{"name":"Hebei Agricultural University , , 289 Lingyusi Road , , ,","place":["Baoding, Hebei, China, 071000"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jie","family":"Gao","sequence":"additional","affiliation":[{"name":"Hebei Agricultural University , , 289 Lingyusi Road , , ,","place":["Baoding, Hebei, China, 071000"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1071-8297","authenticated-orcid":true,"given":"Yaxin","family":"Sang","sequence":"additional","affiliation":[{"name":"Hebei Agricultural University , , 289 Lingyusi Road , , ,","place":["Baoding, Hebei, China, 071000"]}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"316","published-online":{"date-parts":[[2026,8,6]]},"reference":[{"issue":"3","key":"2026081910172962100_cit1","doi-asserted-by":"publisher","first-page":"227","DOI":"10.12938\/bmfh.2024-110","article-title":"Optimization of growth conditions and the inducer concentration\nfor increasing spike protein expression in recombinant Lactococcus lactis and its kinetic modeling","volume":"44","author":"Mubarak","year":"2025","journal-title":"Biosci. 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