{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,16]],"date-time":"2026-09-16T14:05:37Z","timestamp":1789567537191,"version":"build-2803163510"},"reference-count":42,"publisher":"American Chemical Society (ACS)","issue":"36","license":[{"start":{"date-parts":[[2026,9,7]],"date-time":"2026-09-07T00:00:00Z","timestamp":1788739200000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-029"},{"start":{"date-parts":[[2026,9,7]],"date-time":"2026-09-07T00:00:00Z","timestamp":1788739200000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-037"},{"start":{"date-parts":[[2026,9,7]],"date-time":"2026-09-07T00:00:00Z","timestamp":1788739200000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-045"}],"funder":[{"DOI":"10.13039\/501100012166","name":"National Key Research and Development Program of China","doi-asserted-by":"publisher","award":["2023YFD2200501"],"award-info":[{"award-number":["2023YFD2200501"]}],"id":[{"id":"10.13039\/501100012166","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100012176","name":"Project 211","doi-asserted-by":"publisher","award":["B21022"],"award-info":[{"award-number":["B21022"]}],"id":[{"id":"10.13039\/501100012176","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/na","name":"5?5 Engineering Research & Innovation Team Project of Beijing Forestry University","doi-asserted-by":"publisher","award":["BLRC2023B01"],"award-info":[{"award-number":["BLRC2023B01"]}],"id":[{"id":"10.13039\/na","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2026,9,16]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>Efficient biomass utilization is constrained by the trade-off between the cell wall stability and deconstructability. Space-mutated Arundo donax L. (MUT) achieves coordinated improvements in both properties. Here, confocal Raman microscopy imaging, chemical composition analysis, and 2D HSQC NMR were integrated to investigate the lignin-hydroxycinnamic acid (HCA)\u2013xylan (LCC) network. Compared with wild-type (WT), MUT exhibited altered monolignol composition and linkages, resulting in a less condensed lignin network and a more homogeneous LCC network. Raman imaging revealed that lignin, HCA, and xylan in MUT were more susceptible to alkaline treatment, particularly in compound middle lamella (CML). After acidified NaClO2 treatment, lignin and HCA were largely removed from the CML in MUT, and the cross-link network in the secondary wall extensively disassembled, accompanied by a clear reduction in co-localization regions. These findings provide a novel structural perspective for balancing cell wall stability and degradability, laying the theoretical foundation for the targeted breeding of high-quality biomass crops.<\/jats:p>","DOI":"10.1021\/acs.jafc.6c06968","type":"journal-article","created":{"date-parts":[[2026,9,7]],"date-time":"2026-09-07T07:02:55Z","timestamp":1788764575000},"page":"28542-28557","source":"Crossref","is-referenced-by-count":0,"title":["Topochemical Reorganization of the Lignin\u2013HCA\u2013Xylan\nNetwork Governs Cell Wall Deconstructability in Space-Mutated\n                    <i>Arundo donax<\/i>\n                    L."],"prefix":"10.1021","volume":"74","author":[{"ORCID":"https:\/\/orcid.org\/0009-0005-7463-9709","authenticated-orcid":true,"given":"Li","family":"Li","sequence":"first","affiliation":[{"name":"State Key Laboratory of Efficient Production of Forest Resources , ,","place":["Beijing, China, 100083"]},{"name":"Beijing Key Laboratory of Lignocellulosic Chemistry , ,","place":["Beijing, China, 100083"]},{"name":"Beijing Forestry University , ,","place":["Beijing, China, 100083"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shenao","family":"Sun","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Efficient Production of Forest Resources , ,","place":["Beijing, China, 100083"]},{"name":"Beijing Key Laboratory of Lignocellulosic Chemistry , ,","place":["Beijing, China, 100083"]},{"name":"Beijing Forestry University , ,","place":["Beijing, China, 100083"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xun","family":"Zhang","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Efficient Production of Forest Resources , ,","place":["Beijing, China, 100083"]},{"name":"Beijing Key Laboratory of Lignocellulosic Chemistry , ,","place":["Beijing, China, 100083"]},{"name":"Beijing Forestry University , ,","place":["Beijing, China, 100083"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2184-1872","authenticated-orcid":true,"given":"Feng","family":"Xu","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Efficient Production of Forest Resources , ,","place":["Beijing, China, 100083"]},{"name":"Beijing Key Laboratory of Lignocellulosic Chemistry , ,","place":["Beijing, China, 100083"]},{"name":"Beijing Forestry University , ,","place":["Beijing, China, 100083"]}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"316","published-online":{"date-parts":[[2026,9,7]]},"reference":[{"issue":"2","key":"2026091609093964300_cit1","doi-asserted-by":"publisher","first-page":"463","DOI":"10.1093\/jxb\/erv511","article-title":"Plant Cell\nWall Extensibility: Connecting Plant Cell Growth with Cell Wall Structure,\nMechanics, and the Action of Wall-Modifying Enzymes","volume":"67","author":"Cosgrove","year":"2016","journal-title":"J. Exp. Bot."},{"key":"2026091609093964300_cit2","doi-asserted-by":"publisher","first-page":"212","DOI":"10.3390\/plants9020212","article-title":"Plant Cell\nWalls Tackling Climate Change: Biotechnological Strategies to Improve\nCrop Adaptations and Photosynthesis in Response to Global Warming","volume":"9","author":"Ezquer","year":"2020","journal-title":"Plants"},{"key":"2026091609093964300_cit3","doi-asserted-by":"publisher","first-page":"373","DOI":"10.1016\/j.biortech.2019.01.055","article-title":"Overview\nof the Recent Advances in Lignocellulose Liquefaction for Producing\nBiofuels, Bio-Based Materials and Chemicals","volume":"279","author":"Kim","year":"2019","journal-title":"Bioresour. Technol."},{"issue":"1","key":"2026091609093964300_cit4","doi-asserted-by":"publisher","first-page":"27","DOI":"10.1111\/ppl.12705","article-title":"Plant Cell Wall Sugars:\nSweeteners for a Bio-Based Economy","volume":"164","author":"Van de Wouwer","year":"2018","journal-title":"Physiol.\nPlant."},{"issue":"1","key":"2026091609093964300_cit5","doi-asserted-by":"publisher","first-page":"60","DOI":"10.1111\/nph.17383","article-title":"Growth\u2013Defense Trade-Offs\nand Yield Loss in Plants with Engineered Cell Walls","volume":"231","author":"Ha","year":"2021","journal-title":"New Phytol."},{"issue":"5","key":"2026091609093964300_cit6","doi-asserted-by":"publisher","first-page":"997","DOI":"10.1016\/j.biotechadv.2016.06.001","article-title":"Genetic Modification\nof Plant Cell Walls to Enhance Biomass Yield\nand Biofuel Production in Bioenergy Crops","volume":"34","author":"Wang","year":"2016","journal-title":"Biotechnol.\nAdv."},{"key":"2026091609093964300_cit7","doi-asserted-by":"publisher","first-page":"115448","DOI":"10.1016\/j.carbpol.2019.115448","article-title":"Distinct Cellulose\nand Callose Accumulation for Enhanced Bioethanol\nProduction and Biotic Stress Resistance in OsSUS3 Transgenic Rice","volume":"232","author":"Fan","year":"2020","journal-title":"Carbohydr. Polym."},{"issue":"4","key":"2026091609093964300_cit8","doi-asserted-by":"publisher","first-page":"465","DOI":"10.1002\/bbb.1331","article-title":"Biomass recalcitrance.\nPart I: the\nChemical Compositions and Physical Structures Affecting the Enzymatic\nHydrolysis of Lignocellulose","volume":"6","author":"Zhao","year":"2012","journal-title":"Biofuels, Bioprod.\nBiorefin."},{"key":"2026091609093964300_cit9","doi-asserted-by":"publisher","first-page":"763","DOI":"10.1007\/s00425-010-1338-z","article-title":"Functional Plant Cell Wall Design\nRevealed by the Raman Imaging Approach","volume":"233","author":"Richter","year":"2011","journal-title":"Planta"},{"issue":"13","key":"2026091609093964300_cit10","doi-asserted-by":"publisher","first-page":"4997","DOI":"10.1021\/acssuschemeng.0c01109","article-title":"Lignin Monomers from\nBeyond the Canonical Monolignol Biosynthetic Pathway: Another Brick\nin the Wall","volume":"8","author":"del Ri\u0301o","year":"2020","journal-title":"ACS Sustain. Chem. Eng."},{"key":"2026091609093964300_cit11","doi-asserted-by":"publisher","first-page":"269","DOI":"10.1186\/s13068-018-1262-1","article-title":"Lignin\u2013Carbohydrate\nComplexes:\nProperties, Applications, Analyses, and Methods of Extraction: A Review","volume":"11","author":"Tarasov","year":"2018","journal-title":"Biotechnol. Biofuels."},{"issue":"3","key":"2026091609093964300_cit12","doi-asserted-by":"publisher","first-page":"733","DOI":"10.1016\/0031-9422(90)80009-6","article-title":"Phenolic\nAcid Bridges between Polysaccharides\nand Lignin in Wheat Internodes","volume":"29","author":"Iiyama","year":"1990","journal-title":"Phytochemistry"},{"key":"2026091609093964300_cit13","doi-asserted-by":"publisher","first-page":"118817","DOI":"10.1016\/j.carbpol.2021.118817","article-title":"Chlorine Dioxide Oxidation of Hemicellulose\nfrom Alkaline Hydrolysate Bagasse to Remove Lignin Unit in Lignin\u2013Carbohydrate\nComplex","volume":"277","author":"Han","year":"2022","journal-title":"Carbohydr. Polym."},{"key":"2026091609093964300_cit14","doi-asserted-by":"publisher","first-page":"122316","DOI":"10.1016\/j.indcrop.2025.122316","article-title":"Characterization\nof Cell Wall Structure, Stress Resistance, and Biomass\nSaccharification in Space-Mutated Arundo donax L","volume":"237","author":"Li","year":"2025","journal-title":"Ind. Crops Prod."},{"key":"2026091609093964300_cit15","doi-asserted-by":"publisher","first-page":"55910","DOI":"10.3791\/55910-v","article-title":"Combining\nRaman Imaging and Multivariate\nAnalysis to Visualize Lignin, Cellulose, and Hemicellulose in the\nPlant Cell Wall","volume":"124","author":"Zhang","year":"2017","journal-title":"J. Vis. Exp."},{"key":"2026091609093964300_cit16","doi-asserted-by":"publisher","first-page":"29","DOI":"10.1186\/s13007-021-00730-9","article-title":"A Label-Free, Fast\nand High-Specificity Technique for Plant Cell Wall Imaging and Composition\nAnalysis","volume":"17","author":"Xu","year":"2021","journal-title":"Plant Methods"},{"key":"2026091609093964300_cit17","unstructured":"Hames, B.; Ruiz, R.; Scarlata, C.; Sluiter, A.; Sluiter, J.; Templeton, D.\n          Preparation\nof Samples for Compositional\nAnalysis, Laboratory Analytical Procedure (LAP), 2008; Vol. 1617, pp 65\u201371."},{"issue":"12","key":"2026091609093964300_cit18","doi-asserted-by":"publisher","first-page":"11618","DOI":"10.1021\/acssuschemeng.7b02970","article-title":"Structural\nCharacteristics of Lignin Macromolecules from Different Eucalyptus\nSpecies","volume":"5","author":"Wang","year":"2017","journal-title":"ACS Sustain. Chem. Eng."},{"key":"2026091609093964300_cit19","unstructured":"Scarlata, C.; Sluiter, J.; Templeton, D.; Crocker, D.\n          Determination of Structural\nCarbohydrates and Lignin in Biomass, Technical Report NREL\/TP-510-42618; National Renewable Energy Laboratory (NREL): Golden, CO, 2011."},{"key":"2026091609093964300_cit20","doi-asserted-by":"publisher","first-page":"332","DOI":"10.1016\/j.indcrop.2012.05.041","article-title":"Unmasking\nthe Structural Features and Property of Lignin from Bamboo","volume":"42","author":"Wen","year":"2013","journal-title":"Ind. Crops Prod."},{"issue":"23","key":"2026091609093964300_cit21","doi-asserted-by":"publisher","first-page":"5922","DOI":"10.1021\/jf301002n","article-title":"Structural Characterization of Wheat\nStraw Lignin as Revealed by Analytical Pyrolysis, 2D-NMR, and Reductive\nCleavage Methods","volume":"60","author":"del Ri\u0301o","year":"2012","journal-title":"J. Agric. Food Chem."},{"key":"2026091609093964300_cit22","doi-asserted-by":"publisher","first-page":"9529","DOI":"10.1002\/chem.201003045","article-title":"Elucidation of Lignin Structure by\nQuantitative 2D NMR","volume":"17","author":"Sette","year":"2011","journal-title":"Chem.\u2500Eur. J."},{"issue":"3","key":"2026091609093964300_cit23","doi-asserted-by":"publisher","first-page":"956","DOI":"10.1017\/S1431927614000658","article-title":"Raman Microspectroscopy Imaging Study on Topochemical Correlation\nbetween Lignin and Hydroxycinnamic Acids in Miscanthus Sinensis","volume":"20","author":"Ma","year":"2014","journal-title":"Microsc. Microanal."},{"issue":"3","key":"2026091609093964300_cit24","doi-asserted-by":"publisher","first-page":"963","DOI":"10.1016\/S0032-3861(00)00434-1","article-title":"Interactions between Wood Polymers\nStudied by Dynamic\nFT-IR Spectroscopy","volume":"42","author":"\u00c5kerholm","year":"2001","journal-title":"Polymer"},{"issue":"3","key":"2026091609093964300_cit25","doi-asserted-by":"publisher","first-page":"435","DOI":"10.3390\/biom13030435","article-title":"Comparative Analysis\nof G-Layers in Bast Fiber and Xylem Cell Walls in Flax Using Raman\nSpectroscopy","volume":"13","author":"Blervacq","year":"2023","journal-title":"Biomolecules"},{"issue":"18","key":"2026091609093964300_cit26","doi-asserted-by":"publisher","first-page":"8570","DOI":"10.3390\/app11188570","article-title":"Comparative FT-IR Prospecting for\nCellulose in Stems of Some Fiber Plants: Flax, Velvet Leaf, Hemp and\nJute","volume":"11","author":"Va\u0302rban","year":"2021","journal-title":"Appl. Sci."},{"issue":"9","key":"2026091609093964300_cit27","doi-asserted-by":"publisher","first-page":"1659","DOI":"10.3390\/molecules24091659","article-title":"Analysis\nof Cellulose and Lignocellulose Materials by Raman Spectroscopy: A\nReview of the Current Status","volume":"24","author":"Agarwal","year":"2019","journal-title":"Molecules"},{"issue":"6","key":"2026091609093964300_cit28","doi-asserted-by":"publisher","first-page":"859","DOI":"10.1016\/S0031-9422(01)00049-8","article-title":"Lignification\nand Lignin Topochemistry\u2500An Ultrastructural View","volume":"57","author":"Donaldson","year":"2001","journal-title":"Phytochemistry"},{"issue":"14","key":"2026091609093964300_cit29","doi-asserted-by":"publisher","first-page":"4305","DOI":"10.1093\/jxb\/erv016","article-title":"Cell-Wall\nProperties\nContributing to Improved Deconstruction by Alkaline Pre-treatment\nand Enzymatic Hydrolysis in Diverse Maize (Zea mays L.) Lines","volume":"66","author":"Li","year":"2015","journal-title":"J. Exp. Bot."},{"key":"2026091609093964300_cit30","doi-asserted-by":"publisher","first-page":"45","DOI":"10.3389\/fchem.2016.00045","article-title":"Current Understanding of the Correlation of Lignin\nStructure with Biomass Recalcitrance","volume":"4","author":"Li","year":"2016","journal-title":"Front.\nChem."},{"issue":"1","key":"2026091609093964300_cit31","doi-asserted-by":"publisher","first-page":"65","DOI":"10.1007\/s11101-009-9141-9","article-title":"Hydroxycinnamates\nin Lignification","volume":"9","author":"Ralph","year":"2010","journal-title":"Phytochem. Rev."},{"issue":"1","key":"2026091609093964300_cit32","doi-asserted-by":"publisher","first-page":"5","DOI":"10.3390\/fib12010005","article-title":"Raman Spectra of\nDelignified Plant Fibers: Exploring\nthe Impact of Xylan\u2019s Presence on the Spectral Features of\nCellulose","volume":"12","author":"Agarwal","year":"2024","journal-title":"Fibers"},{"issue":"3","key":"2026091609093964300_cit33","doi-asserted-by":"publisher","first-page":"179","DOI":"10.1016\/S0926-6690(01)00112-1","article-title":"Ester and\nEther Linkages between Hydroxycinnamic Acids and Lignins from Wheat,\nRice, Rye, and Barley Straws, Maize Stems, and Fast-Growing Poplar\nWood","volume":"15","author":"Sun","year":"2002","journal-title":"Ind. Crops Prod."},{"key":"2026091609093964300_cit34","doi-asserted-by":"publisher","first-page":"319","DOI":"10.1007\/s12649-024-02567-4","article-title":"The Effect\nof Oxidative Bleaching\nUsing Chlorine Oxoacid Agents on the Characteristics of Cellulose\nFibers and Hydrogel Films of Sugarcane Bagasse","volume":"16","author":"Ibaraki","year":"2025","journal-title":"Waste Biomass Valor."},{"key":"2026091609093964300_cit35","doi-asserted-by":"publisher","first-page":"154018","DOI":"10.1016\/j.cej.2024.154018","article-title":"Structural Evolution and Reaction\nKinetics of Lignin-Carbohydrate Complex Oxidized by Chlorine Dioxide","volume":"496","author":"Wang","year":"2024","journal-title":"Chem. Eng. J."},{"key":"2026091609093964300_cit36","doi-asserted-by":"publisher","first-page":"149","DOI":"10.1016\/S0921-0423(01)80067-8","article-title":"On the Mechanism\nto Regulate the Ratio of Syringyl to Guaiacyl Moieties in Lignin","volume-title":"Progress in Biotechnology","author":"Fukushima","year":"2001"},{"key":"2026091609093964300_cit37","doi-asserted-by":"publisher","first-page":"188","DOI":"10.12677\/BR.2014.35024","article-title":"Study on Microscopy\nTechniques for\nIdentifying Lignin Distribution in the Stem of Sorghum","volume":"3","author":"Deng","year":"2014","journal-title":"Bot. Res."},{"issue":"50","key":"2026091609093964300_cit38","doi-asserted-by":"publisher","first-page":"10747","DOI":"10.1021\/acs.jafc.5b04831","article-title":"Unraveling\nthe Structural Modifications in Lignin of Arundo donax Linn. During\nAcid-Enhanced Ionic Liquid Pretreatment","volume":"63","author":"You","year":"2015","journal-title":"J.\nAgric. Food Chem."},{"issue":"5","key":"2026091609093964300_cit39","doi-asserted-by":"publisher","first-page":"2163","DOI":"10.1111\/nph.70347","article-title":"Stacked Mutations\nDisrupting Syringyl\nand P-Coumaroylated Lignin Biosynthesis Result in Lignin Dominated\nby Guaiacyl Units in Rice","volume":"247","author":"Ji","year":"2025","journal-title":"New Phytol."},{"key":"2026091609093964300_cit40","doi-asserted-by":"publisher","first-page":"309","DOI":"10.1016\/j.indcrop.2016.03.012","article-title":"Wheat Straw Lignin Fractionation\nand Characterization as Lignin-Carbohydrate\nComplexes","volume":"85","author":"Zikeli","year":"2016","journal-title":"Ind. Crops Prod."},{"key":"2026091609093964300_cit41","doi-asserted-by":"publisher","first-page":"121475","DOI":"10.1016\/j.biortech.2019.121475","article-title":"Subcellular Dissolution of Xylan and Lignin for Enhancing Enzymatic\nHydrolysis of Microwave Assisted Deep Eutectic Solvent Pretreated\nPinus bungeana Zucc","volume":"288","author":"Li","year":"2019","journal-title":"Bioresour. Technol."},{"issue":"5","key":"2026091609093964300_cit42","doi-asserted-by":"publisher","first-page":"1152","DOI":"10.1111\/tpj.15620","article-title":"Hydroxycinnamic Acid-Modified\nXylan Side Chains and Their Cross-Linking Products in Rice Cell Walls\nare Reduced in the Xylosyl Arabinosyl Substitution Of Xylan 1 Mutant","volume":"109","author":"Feijao","year":"2022","journal-title":"Plant J."}],"container-title":["Journal of Agricultural\nand Food Chemistry"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/pubs.acs.org\/doi\/pdf\/10.1021\/acs.jafc.6c06968","content-type":"application\/pdf","content-version":"vor","intended-application":"unspecified"},{"URL":"https:\/\/pubs.acs.org\/jafcau\/article-pdf\/74\/36\/28542\/68399555\/acs.jafc.6c06968.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"syndication"},{"URL":"https:\/\/pubs.acs.org\/jafcau\/article-pdf\/74\/36\/28542\/68399555\/acs.jafc.6c06968.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,9,16]],"date-time":"2026-09-16T13:19:38Z","timestamp":1789564778000},"score":1,"resource":{"primary":{"URL":"https:\/\/pubs.acs.org\/jafcau\/article\/74\/36\/28542\/5422110\/Topochemical-Reorganization-of-the-Lignin-HCA"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,9,7]]},"references-count":42,"journal-issue":{"issue":"36","published-online":{"date-parts":[[2026,9,7]]},"published-print":{"date-parts":[[2026,9,16]]}},"URL":"https:\/\/doi.org\/10.1021\/acs.jafc.6c06968","relation":{},"ISSN":["0021-8561","1520-5118"],"issn-type":[{"value":"0021-8561","type":"print"},{"value":"1520-5118","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026,9,7]]}}}