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In this work, Candida rugosa lipase (CRL) was precisely immobilized on magnetic chitosan carriers functionalized with amino acid ionic liquids (AAIL), forming a stable biocatalytic platform. The immobilized lipase showed significantly improved thermal stability, organic solvent resistance, and storage stability. Molecular dynamics simulations revealed that the carrier enhanced enzyme rigidity, while AAIL regulated enzyme lid movement, optimized catalytic site distances, and stabilized the catalytic triad, increasing the active conformation ratio from 37% to 68%. Applied in phytosterol ester synthesis, it achieved a 94.4% esterification yield and retained nearly 70% efficiency after six cycles. This work provides guidance for high-efficiency biocatalysts and green biocatalysis development.<\/jats:p>","DOI":"10.1021\/acs.jafc.6c07095","type":"journal-article","created":{"date-parts":[[2026,8,4]],"date-time":"2026-08-04T13:11:39Z","timestamp":1785849099000},"page":"23684-23697","source":"Crossref","is-referenced-by-count":0,"title":["Amino Acid Ionic\nLiquid-Functionalized Magnetic Interface-Induced\nDirectional Enrichment and Immobilization of Active Conformations\nof Lipase"],"prefix":"10.1021","volume":"74","author":[{"given":"Wei","family":"Zhang","sequence":"first","affiliation":[{"name":"Huaibei Normal University , , ,","place":["Huaibei, China, 235000"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ziyi","family":"Li","sequence":"additional","affiliation":[{"name":"Huaibei Normal University , , ,","place":["Huaibei, China, 235000"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Fan","family":"Li","sequence":"additional","affiliation":[{"name":"Huaibei Normal University , , ,","place":["Huaibei, China, 235000"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jian","family":"Wang","sequence":"additional","affiliation":[{"name":"Huaibei Normal University , , ,","place":["Huaibei, China, 235000"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zeping","family":"Lu","sequence":"additional","affiliation":[{"name":"Nanjing Tech University , , ,","place":["Nanjing, China, 210009"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4238-3268","authenticated-orcid":true,"given":"Yongxing","family":"Zhang","sequence":"additional","affiliation":[{"name":"Huaibei Normal University , , ,","place":["Huaibei, China, 235000"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3080-4571","authenticated-orcid":true,"given":"Yi","family":"Hu","sequence":"additional","affiliation":[{"name":"Nanjing Tech University , , ,","place":["Nanjing, China, 210009"]}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"316","published-online":{"date-parts":[[2026,7,27]]},"reference":[{"issue":"15","key":"2026080510101523000_cit1","doi-asserted-by":"publisher","DOI":"10.1002\/smll.202512609","article-title":"Rigid-Flexible Layered\nImmobilization Enables Precise Confinement and Dynamic Activation\nof Small Enzymes","volume":"22","author":"Zhu","year":"2026","journal-title":"Small"},{"issue":"34","key":"2026080510101523000_cit2","doi-asserted-by":"publisher","first-page":"15111","DOI":"10.1021\/acs.est.4c02849","article-title":"Laccase Immobilized on Arginine-Functionalized Boron\nNitride Nanosheets for Enhanced Atrazine Degradation","volume":"58","author":"Gao","year":"2024","journal-title":"Environ. 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