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In this study, a novel UDP-dependent glycosyltransferase, SoUGT, was identified from Saponaria officinalis for regioselective glycosylation of piceatannol. SoUGT belongs to the UGT72 subfamily and contains the conserved PSPG (Plant Secondary Product Glycosyltransferase) motif typical of plant O-glycosyltransferases. Molecular docking analysis predicted several residues, including Tyr143, Val120, Ala121, and Val123, that may contribute to substrate binding and positioning. GST fusion significantly improved soluble expression and catalytic activity in Escherichia coli. The enzyme showed optimal activity at 50 \u00b0C and pH 8.5, with good thermal stability and solvent tolerance, predominantly producing piceatannol-4\u2032-O-glucoside. To reduce UDP-glucose cost, a cellobiose-based regeneration system was developed. Under optimized conditions, the engineered strain produced 380.84 mg\/L piceatannol-4\u2032-O-glucoside within 48 h, achieving a 78.77% conversion rate.<\/jats:p>","DOI":"10.1021\/acs.jafc.6c07316","type":"journal-article","created":{"date-parts":[[2026,8,18]],"date-time":"2026-08-18T17:02:29Z","timestamp":1787072549000},"page":"26517-26531","source":"Crossref","is-referenced-by-count":0,"title":["Biocatalytic Production\nof Piceatannol-4\u2032-O-Glucoside\nUsing a Novel Glycosyltransferase SoUGT from\n                    <i>Saponaria officinalis<\/i>"],"prefix":"10.1021","volume":"74","author":[{"given":"Jiaqi","family":"Xu","sequence":"first","affiliation":[{"name":"Nanjing Forestry University , , ,","place":["Nanjing, China, 210037"]},{"name":"Nanjing Forestry University , , ,","place":["Nanjing, China, 210037"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jin","family":"Xu","sequence":"additional","affiliation":[{"name":"Nanjing Forestry University , , ,","place":["Nanjing, China, 210037"]},{"name":"Nanjing Forestry University , , ,","place":["Nanjing, China, 210037"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xianying","family":"Fang","sequence":"additional","affiliation":[{"name":"Nanjing Forestry University , , ,","place":["Nanjing, China, 210037"]},{"name":"Nanjing Forestry University , , ,","place":["Nanjing, China, 210037"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kaixuan","family":"Ke","sequence":"additional","affiliation":[{"name":"Nanjing Forestry University , , ,","place":["Nanjing, China, 210037"]},{"name":"Nanjing Forestry University , , ,","place":["Nanjing, China, 210037"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Changning","family":"Lu","sequence":"additional","affiliation":[{"name":"Nanjing Forestry University , , ,","place":["Nanjing, China, 210037"]},{"name":"Nanjing Forestry University , , ,","place":["Nanjing, China, 210037"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6713-0016","authenticated-orcid":true,"given":"Jianjun","family":"Pei","sequence":"additional","affiliation":[{"name":"Nanjing Forestry University , , ,","place":["Nanjing, China, 210037"]},{"name":"Nanjing Forestry University , , ,","place":["Nanjing, China, 210037"]},{"name":"Nanjing Forestry University , , ,","place":["Nanjing, China, 210037"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Qi","family":"Li","sequence":"additional","affiliation":[{"name":"Nanjing Forestry University , , ,","place":["Nanjing, China, 210037"]},{"name":"Nanjing Forestry University , , ,","place":["Nanjing, China, 210037"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0005-8102-7970","authenticated-orcid":true,"given":"Linguo","family":"Zhao","sequence":"additional","affiliation":[{"name":"Nanjing Forestry University , , ,","place":["Nanjing, China, 210037"]},{"name":"Nanjing Forestry University , , ,","place":["Nanjing, China, 210037"]},{"name":"Nanjing Forestry University , , ,","place":["Nanjing, China, 210037"]}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"316","published-online":{"date-parts":[[2026,8,18]]},"reference":[{"issue":"4","key":"2026090104465307800_cit1","doi-asserted-by":"publisher","first-page":"837","DOI":"10.1007\/s13596-025-00845-7","article-title":"Piceatannol:\nA Multifaceted Polyphenol\u2500from Pharmacological Actions to Formulation\nDevelopment and Clinical Trials","volume":"25","author":"Rajan","year":"2025","journal-title":"Adv. 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Biotechnol."},{"issue":"15","key":"2026090104465307800_cit43","doi-asserted-by":"publisher","first-page":"6587","DOI":"10.1007\/s00253-020-10667-z","article-title":"Advances on the In Vivo and in Vitro Glycosylations\nof Flavonoids","volume":"104","author":"Ji","year":"2020","journal-title":"Appl. Microbiol. Biotechnol."},{"issue":"32","key":"2026090104465307800_cit44","doi-asserted-by":"publisher","first-page":"9249","DOI":"10.1021\/acs.jafc.1c02602","article-title":"Dual Strategy for Improving the Oral\nBioavailability of Resveratrol: Enhancing Water Solubility and Inhibiting\nGlucuronidation","volume":"69","author":"Li","year":"2021","journal-title":"J. Agric. 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