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In this study, Xinjunan (dioctyldiethylenetriamine) exerts potent antibacterial activity against Psa (EC50 = 4.29 mg\/L), outperforming thiodiazole-copper (34.8 mg\/L). Transcriptomic analysis showed that Xinjunan affected pathways associated with flagellar assembly, biofilm formation, membrane biogenesis, and chemotaxis. Xinjunan treatment also induced reactive oxygen species (ROS) accumulation, leading to bacterial death. Deletion of FleQ enhances Psa resistance to Xinjunan, reduces virulence, and inhibits flagellar formation. Under Xinjunan treatment, the mutant strain showed better growth than the wild-type strain, with no significant differences in motility capacity or ROS levels; the complemented strain restored sensitivity to Xinjunan. Microscale thermophoresis and isothermal titration calorimetry determined dissociation constants of Xinjunan binding to the FleQ protein as 1.64 and 6.41 \u03bcM, respectively. Molecular docking and molecular dynamics simulations identified FleQ Glu264 as a critical binding residue. These findings provide promising targets for the management of bacterial phytopathogen Psa.<\/jats:p>","DOI":"10.1021\/acs.jafc.6c07386","type":"journal-article","created":{"date-parts":[[2026,9,22]],"date-time":"2026-09-22T03:02:01Z","timestamp":1790046121000},"page":"30549-30560","source":"Crossref","is-referenced-by-count":0,"title":["Xinjunan Binds\nto\n                    <i>Pseudomonas syringae<\/i>\n                    Pv.\n                    <i>Actinidiae<\/i>\n                    FleQ to Inhibit Flagella and Biofilm\nFormation and Reduce Pathogenicity"],"prefix":"10.1021","volume":"74","author":[{"given":"Zhaojia","family":"Li","sequence":"first","affiliation":[{"name":"Center for R&D of Fine Chemicals of Guizhou University , , ,","place":["Guiyang, China, 550025"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xin","family":"Luo","sequence":"additional","affiliation":[{"name":"Center for R&D of Fine Chemicals of Guizhou University , , ,","place":["Guiyang, China, 550025"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Na","family":"Tao","sequence":"additional","affiliation":[{"name":"Center for R&D of Fine Chemicals of Guizhou University , , ,","place":["Guiyang, China, 550025"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shuai","family":"Zhu","sequence":"additional","affiliation":[{"name":"Center for R&D of Fine Chemicals of Guizhou University , , ,","place":["Guiyang, China, 550025"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sheng","family":"Wang","sequence":"additional","affiliation":[{"name":"Center for R&D of Fine Chemicals of Guizhou University , , ,","place":["Guiyang, China, 550025"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jing","family":"Liu","sequence":"additional","affiliation":[{"name":"Center for R&D of Fine Chemicals of Guizhou University , , ,","place":["Guiyang, China, 550025"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4538-5533","authenticated-orcid":true,"given":"Moxian","family":"Chen","sequence":"additional","affiliation":[{"name":"Center for R&D of Fine Chemicals of Guizhou University , , ,","place":["Guiyang, China, 550025"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jingjiang","family":"Zhou","sequence":"additional","affiliation":[{"name":"Center for R&D of Fine Chemicals of Guizhou University , , ,","place":["Guiyang, China, 550025"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1502-1811","authenticated-orcid":true,"given":"Xiangyang","family":"Li","sequence":"additional","affiliation":[{"name":"Center for R&D of Fine Chemicals of Guizhou University , , ,","place":["Guiyang, China, 550025"]}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"316","published-online":{"date-parts":[[2026,9,21]]},"reference":[{"issue":"45","key":"2026100711071943400_cit1","doi-asserted-by":"publisher","first-page":"12558","DOI":"10.1021\/acs.jafc.0c02528","article-title":"Antibacterial functions\nand proposed modes of action of novel 1,2,3,4-tetrahydro-\u03b2-carboline\nderivatives that possess an attractive 1,3-diaminopropan-2-ol pattern\nagainst rice bacterial blight, kiwifruit bacterial canker, and citrus\nbacterial canker","volume":"68","author":"Liu","year":"2020","journal-title":"J. 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