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The flower-like MnO2 interlayer provides chemical shielding, while mesoporous channels were expanded to 6.45 nm to geometrically match CRL (approximately 5 nm). Driven by interfacial activation, the support exhibited a selective purification effect, achieving a high activity-based immobilization yield (85.2%) and protein loading (143.1 mg g\u20131). The immobilized specific activity reached 31.4 U mg\u20131 (1.57-fold higher than the free enzyme) with 88.2% recovered activity. Crucially, in the sterically demanding esterification of phytosterols, the biocatalyst achieved 84.33% maximum conversion at 50 \u00b0C within 40 h. Furthermore, the rigid silica walls exerted a spatial \u201ccage effect\u201d that locked the enzyme active conformation, conferring exceptional thermal stability and reusability. This hierarchical support design offers a robust solution for macromolecular industrial biocatalysis.<\/jats:p>","DOI":"10.1021\/acs.jafc.6c07410","type":"journal-article","created":{"date-parts":[[2026,7,17]],"date-time":"2026-07-17T02:39:31Z","timestamp":1784255971000},"page":"23056-23068","source":"Crossref","is-referenced-by-count":0,"title":["Size-Tailored Nanospaces\nin Hierarchical Magnetic\nCore\u2013Shell Microspheres for Lipase Immobilization and Phytosterol\nEster Synthesis"],"prefix":"10.1021","volume":"74","author":[{"given":"Yuqi","family":"Fan","sequence":"first","affiliation":[{"name":"Hefei University of Technology , , ,","place":["Hefei, China, 230009"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zhonglin","family":"He","sequence":"additional","affiliation":[{"name":"Hefei University of Technology , , ,","place":["Hefei, China, 230009"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4340-4836","authenticated-orcid":true,"given":"Jin","family":"Mao","sequence":"additional","affiliation":[{"name":"Oil Crops Research Institute, Chinese Academy of Agricultural Sciences , , ,","place":["Wuhan, China, 430062"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5044-9047","authenticated-orcid":true,"given":"Qianchun","family":"Deng","sequence":"additional","affiliation":[{"name":"Oil Crops Research Institute, Chinese Academy of Agricultural Sciences , , ,","place":["Wuhan, China, 430062"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0706-8863","authenticated-orcid":true,"given":"Baocai","family":"Xu","sequence":"additional","affiliation":[{"name":"Hefei University of Technology , , ,","place":["Hefei, China, 230009"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9521-3073","authenticated-orcid":true,"given":"Jie","family":"Shi","sequence":"additional","affiliation":[{"name":"Hefei University of Technology , , ,","place":["Hefei, China, 230009"]}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"316","published-online":{"date-parts":[[2026,7,16]]},"reference":[{"issue":"2","key":"2026072910062588700_cit1","doi-asserted-by":"publisher","first-page":"180","DOI":"10.1016\/j.biotechadv.2005.09.003","article-title":"Understanding Candida Rugosa Lipases: An Overview","volume":"24","author":"Domi\u0301nguez De Mari\u0301a","year":"2006","journal-title":"Biotechnol. 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