{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,9]],"date-time":"2026-09-09T22:07:32Z","timestamp":1788991652531,"version":"build-2803163510"},"reference-count":29,"publisher":"American Chemical Society (ACS)","issue":"35","license":[{"start":{"date-parts":[[2026,8,29]],"date-time":"2026-08-29T00:00:00Z","timestamp":1787961600000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-029"},{"start":{"date-parts":[[2026,8,29]],"date-time":"2026-08-29T00:00:00Z","timestamp":1787961600000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-037"},{"start":{"date-parts":[[2026,8,29]],"date-time":"2026-08-29T00:00:00Z","timestamp":1787961600000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-045"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2026,9,9]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>Food-derived bioactive peptides are promising alternatives to conventional xanthine oxidase (XO) inhibitors, yet individual XO inhibitory peptides from sesame protein remain uncharacterized. Here, sesame and peanut protein isolates were screened with four proteases; the trypsin hydrolyzate of sesame (TSH, 120 min) showed the highest XO inhibition (47.16%). Eight 11S globulin peptides were identified by nanoUHPLC-ESI-Q-TOF MS\/MS. QGDIVAIPSGAAHW (IC50 = 467.2 \u03bcM) and AFYLAGGVPR (IC50 = 536.1 \u03bcM) exhibited mixed-type inhibition (\u03b1 &amp;gt; 1). Molecular docking against two XO crystal structures (3NVY, 3NRZ) consistently favored the FAD domain, and 500 ns molecular dynamics simulations with MM-GBSA analysis provided convergent support (\u0394G\u202f=\u202f\u2013160 to \u2212317 kJ\u202fmol\u20131). In a zebrafish hyperuricemia model, TSH reduced uric acid and XO activity while upregulating purine salvage (hprt1) and urate transport (oat1) genes. These findings support sesame-derived peptides as functional food candidates with preferential association with the XO FAD domain.<\/jats:p>","DOI":"10.1021\/acs.jafc.6c07558","type":"journal-article","created":{"date-parts":[[2026,9,2]],"date-time":"2026-09-02T12:20:43Z","timestamp":1788351643000},"page":"27608-27620","source":"Crossref","is-referenced-by-count":0,"title":["Xanthine Oxidase\nInhibitory Peptides from Sesame 11S\nGlobulin Target the FAD Domain: Kinetics, Molecular Dynamics, and\nValidation in a Zebrafish Hyperuricemia Model"],"prefix":"10.1021","volume":"74","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-7194-7555","authenticated-orcid":true,"given":"Mei-Ling","family":"Li","sequence":"first","affiliation":[{"name":"China Medical University , , 100 Jingmao Road Sec. 1 , Beitun District, ,","place":["Taichung, Taiwan, 406040"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2470-1376","authenticated-orcid":true,"given":"Shang-Ming","family":"Huang","sequence":"additional","affiliation":[{"name":"China Medical University , , 100 Jingmao Road Sec. 1 , Beitun District, ,","place":["Taichung, Taiwan, 406040"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yu-Shun","family":"Lin","sequence":"additional","affiliation":[{"name":"China Medical University , , 100 Jingmao Road Sec. 1 , Beitun District, ,","place":["Taichung, Taiwan, 406040"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Pei-Yu","family":"Wu","sequence":"additional","affiliation":[{"name":"China Medical University , , 100 Jingmao Road Sec. 1 , Beitun District, ,","place":["Taichung, Taiwan, 406040"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yu-Hsuan","family":"Chen","sequence":"additional","affiliation":[{"name":"China Medical University , , 100 Jingmao Road Sec. 1 , Beitun District, ,","place":["Taichung, Taiwan, 406040"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yi-Jing","family":"Chuang","sequence":"additional","affiliation":[{"name":"China Medical University , , 100 Jingmao Road Sec. 1 , Beitun District, ,","place":["Taichung, Taiwan, 406040"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9019-2178","authenticated-orcid":true,"given":"Jiann-Jou","family":"Yang","sequence":"additional","affiliation":[{"name":"Chung Shan Medical University , , 110 Jianguo North Road Sec. 1 , ,","place":["Taichung, Taiwan, 402"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3449-8447","authenticated-orcid":true,"given":"Kuo-Chiang","family":"Hsu","sequence":"additional","affiliation":[{"name":"China Medical University , , 100 Jingmao Road Sec. 1 , Beitun District, ,","place":["Taichung, Taiwan, 406040"]},{"name":"Asia University , , 500 Lioufeng Road , Wufeng, ,","place":["Taichung, Taiwan, 41354"]}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"316","published-online":{"date-parts":[[2026,8,29]]},"reference":[{"key":"2026090917072717200_cit1","doi-asserted-by":"publisher","first-page":"991","DOI":"10.1002\/art.40807","article-title":"Contemporary Prevalence of Gout and\nHyperuricemia in\nthe United States and Decadal Trends: The National Health and Nutrition\nExamination Survey, 2007\u20132016","volume":"71","author":"Chen-Xu","year":"2019","journal-title":"Arthritis\nRheumatol."},{"key":"2026090917072717200_cit2","doi-asserted-by":"publisher","first-page":"1843","DOI":"10.1016\/S0140-6736(21)00569-9","article-title":"Gout","volume":"397","author":"Dalbeth","year":"2021","journal-title":"Lancet"},{"issue":"1","key":"2026090917072717200_cit3","doi-asserted-by":"publisher","first-page":"3527579","DOI":"10.1155\/2016\/3527579","article-title":"Xanthine Oxidoreductase-Derived\nReactive Species: Physiological and Pathological Effects","volume":"2016","author":"Battelli","year":"2016","journal-title":"Oxid. Med. Cell. Longevity"},{"key":"2026090917072717200_cit4","doi-asserted-by":"publisher","first-page":"101501","DOI":"10.1016\/j.berh.2020.101501","article-title":"Allopurinol Hypersensitivity: Pathogenesis and Prevention","volume":"34","author":"Stamp","year":"2020","journal-title":"Best Pract. Res., Clin. Rheumatol."},{"key":"2026090917072717200_cit5","doi-asserted-by":"publisher","first-page":"1200","DOI":"10.1056\/NEJMoa1710895","article-title":"Cardiovascular\nSafety of Febuxostat or Allopurinol in Patients with Gout","volume":"378","author":"White","year":"2018","journal-title":"N. Engl. J. Med."},{"issue":"10264","key":"2026090917072717200_cit6","doi-asserted-by":"publisher","first-page":"1745","DOI":"10.1016\/S0140-6736(20)32234-0","article-title":"Long-term cardiovascular safety of\nfebuxostat compared with allopurinol in patients with gout (FAST):\na multicentre, prospective, randomised, open-label, non-inferiority\ntrial","volume":"396","author":"Mackenzie","year":"2020","journal-title":"Lancet"},{"key":"2026090917072717200_cit7","doi-asserted-by":"publisher","DOI":"10.1136\/bmjopen-2017-017542","article-title":"Rate of Adherence to\nUrate-Lowering Therapy among Patients with Gout: A Systematic Review\nand Meta-Analysis","volume":"8","author":"Yin","year":"2018","journal-title":"BMJ. 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Technol."},{"key":"2026090917072717200_cit11","doi-asserted-by":"publisher","first-page":"139444","DOI":"10.1016\/j.foodchem.2024.139444","article-title":"Food-Derived Bioactive\nPeptides with\nAnti-Hyperuricemic Activity: A Comprehensive Review","volume":"451","author":"Mehmood","year":"2024","journal-title":"Food Chem."},{"key":"2026090917072717200_cit12","first-page":"1287","article-title":"Sesame Proteins","volume":"19","author":"Onsaard","year":"2012","journal-title":"Int. Food Res. 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Chem."},{"key":"2026090917072717200_cit27","doi-asserted-by":"publisher","first-page":"1045","DOI":"10.1093\/bib\/bbv008","article-title":"Docking Small Peptides Remains a\nGreat Challenge: An\nAssessment Using AutoDock Vina","volume":"16","author":"Rentzsch","year":"2015","journal-title":"Brief Bioinform."},{"issue":"S13","key":"2026090917072717200_cit28","doi-asserted-by":"publisher","first-page":"426","DOI":"10.1186\/s12859-018-2449-y","article-title":"Benchmarking\nof Different Molecular Docking Methods\nfor Protein-Peptide Docking","volume":"19","author":"Agrawal","year":"2019","journal-title":"BMC Bioinf."},{"key":"2026090917072717200_cit29","doi-asserted-by":"publisher","first-page":"1554","DOI":"10.1016\/j.jaci.2007.03.041","article-title":"Identification of\n2 New Sesame Seed Allergens: Ses i 6 and Ses i 7","volume":"119","author":"Beyer","year":"2007","journal-title":"J. Allergy Clin. Immunol."}],"container-title":["Journal of Agricultural\nand Food Chemistry"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/pubs.acs.org\/doi\/pdf\/10.1021\/acs.jafc.6c07558","content-type":"application\/pdf","content-version":"vor","intended-application":"unspecified"},{"URL":"https:\/\/pubs.acs.org\/jafcau\/article-pdf\/74\/35\/27608\/67739493\/acs.jafc.6c07558.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"syndication"},{"URL":"https:\/\/pubs.acs.org\/jafcau\/article-pdf\/74\/35\/27608\/67739493\/acs.jafc.6c07558.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,9,9]],"date-time":"2026-09-09T21:17:36Z","timestamp":1788988656000},"score":1,"resource":{"primary":{"URL":"https:\/\/pubs.acs.org\/jafcau\/article\/74\/35\/27608\/5361409\/Xanthine-Oxidase-Inhibitory-Peptides-from-Sesame"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,8,29]]},"references-count":29,"journal-issue":{"issue":"35","published-online":{"date-parts":[[2026,8,29]]},"published-print":{"date-parts":[[2026,9,9]]}},"URL":"https:\/\/doi.org\/10.1021\/acs.jafc.6c07558","relation":{},"ISSN":["0021-8561","1520-5118"],"issn-type":[{"value":"0021-8561","type":"print"},{"value":"1520-5118","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026,8,29]]}}}