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Here, a region-focused engineering strategy was applied to a thermophilic GH10 xylanase to simultaneously improve both properties. The strategy integrates qProtein-guided hydrophobic cluster design for scaffold stabilization and dynamic loop analysis for active-site optimization. The resulting triple mutant A206S\u2013N209D\u2013F130L exhibited substantially improved thermostability, with a 5.79 \u00b0C increase in melting temperature and an 18.8-fold extension of the half-life at 60 \u00b0C. Its optimum temperature increased from 60 to 70 \u00b0C, accompanied by a 129.4% enhancement in catalytic activity at 70 \u00b0C relatively to the wild type. Molecular dynamics simulations indicated that these mutations reshape the conformational energy landscape by stabilizing hydrophobic packing and modulating loop dynamics. This study provides a generalizable framework for simultaneously improving enzyme stability and catalytic performance.<\/jats:p>","DOI":"10.1021\/acs.jafc.6c07704","type":"journal-article","created":{"date-parts":[[2026,8,6]],"date-time":"2026-08-06T16:01:24Z","timestamp":1786032084000},"page":"25476-25489","source":"Crossref","is-referenced-by-count":0,"title":["Dual Engineering\nof the Hydrophobic Core and Functional\nLoop Reshapes the Conformational Energy Landscape for Significantly\nEnhanced Xylanase Activity and Thermostability"],"prefix":"10.1021","volume":"74","author":[{"given":"Zhaoran","family":"Li","sequence":"first","affiliation":[{"name":"Shandong University , , ,","place":["Qingdao, China, 266237"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3682-8307","authenticated-orcid":true,"given":"Zhixin","family":"Dou","sequence":"additional","affiliation":[{"name":"Shandong University , , ,","place":["Qingdao, China, 266237"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0004-1422-4782","authenticated-orcid":true,"given":"Sha","family":"Zhao","sequence":"additional","affiliation":[{"name":"Shandong University , , ,","place":["Qingdao, China, 266237"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0009-2325-2001","authenticated-orcid":true,"given":"Mengyu","family":"Liu","sequence":"additional","affiliation":[{"name":"Shandong University , , ,","place":["Qingdao, China, 266237"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5287-5218","authenticated-orcid":true,"given":"Xiuyun","family":"Wu","sequence":"additional","affiliation":[{"name":"Shandong University , , ,","place":["Qingdao, China, 266237"]},{"name":"Weifang Institute of Shandong University , ,","place":["Weifang, China, 261206"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0000-8159-6886","authenticated-orcid":true,"given":"Lushan","family":"Wang","sequence":"additional","affiliation":[{"name":"Shandong University , , ,","place":["Qingdao, China, 266237"]}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"316","published-online":{"date-parts":[[2026,8,6]]},"reference":[{"issue":"1","key":"2026081910190177400_cit1","doi-asserted-by":"publisher","first-page":"88","DOI":"10.1002\/anie.202006648","article-title":"Biocatalysis:\nEnzymatic Synthesis for Industrial Applications","volume":"60","author":"Wu","year":"2021","journal-title":"Angew. 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Nutr."},{"issue":"22","key":"2026081910190177400_cit4","doi-asserted-by":"publisher","first-page":"7941","DOI":"10.1080\/10408398.2023.2193982","article-title":"Encapsulation of\nEnzymes in Food Industry Using Spray Drying: Recent Advances and Process\nScale-Ups","volume":"64","author":"Weng","year":"2024","journal-title":"Crit. Rev. Food Sci. Nutr."},{"issue":"5","key":"2026081910190177400_cit5","doi-asserted-by":"publisher","first-page":"525","DOI":"10.1080\/07373937.2018.1482908","article-title":"Gaining Insight\non Spray Drying Behavior of Foods via Single Droplet Drying Analyses","volume":"37","author":"Schutyser","year":"2019","journal-title":"Dry. 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