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This study identified a novel XyZHD exhibiting higher catalytic activity toward \u03b1-ZAL and \u03b1-ZEL than ZEN under the assay conditions. Recombinant XyZHD showed optimal activity at pH 9.0 and 45 \u00b0C, and its activity was significantly affected by Zn2+ and Fe3+. Structural analysis combined with mutagenesis studies provided insights into residues associated with catalytic activity. The S153E mutant showed a 3.1-fold increase in activity compared with the wild type. XyZHD exhibited high specific activity toward ZEN derivatives, favorable thermal stability, and efficient degradation of \u03b1-ZAL in corn grits, suggesting its potential for further research and development in the field of ZEN biocontrol.<\/jats:p>","DOI":"10.1021\/acs.jafc.6c07705","type":"journal-article","created":{"date-parts":[[2026,9,3]],"date-time":"2026-09-03T17:19:42Z","timestamp":1788455982000},"page":"28752-28766","source":"Crossref","is-referenced-by-count":0,"title":["A Novel Hydrolase\nCapable of Efficiently Degrading\nthe Highly Toxic ZEN Derivative"],"prefix":"10.1021","volume":"74","author":[{"given":"Mingzhu","family":"Yan","sequence":"first","affiliation":[{"name":"Tianjin University of Science and Technology , , No 9, 13th, Avenue, Tianjin Economic and Technological Development Area , ,","place":["Tianjin, China, 300457"]},{"name":"Tianjin Institute of Industrial Biotechnology, Chinese Academy of Sciences , , ,","place":["Tianjin, China, 300308"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9712-6897","authenticated-orcid":true,"given":"Jian","family":"Gao","sequence":"additional","affiliation":[{"name":"Tianjin Institute of Industrial Biotechnology, Chinese Academy of Sciences , , ,","place":["Tianjin, China, 300308"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Qianli","family":"Zhang","sequence":"additional","affiliation":[{"name":"Tianjin Institute of Industrial Biotechnology, Chinese Academy of Sciences , , ,","place":["Tianjin, China, 300308"]},{"name":"University of Chinese Academy of Sciences , 19A Yuquan Road , ,","place":["Beijing, China, 100049"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiao","family":"Liang","sequence":"additional","affiliation":[{"name":"Tianjin Institute of Industrial Biotechnology, Chinese Academy of Sciences , , ,","place":["Tianjin, China, 300308"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2588-3634","authenticated-orcid":true,"given":"Huimin","family":"Qin","sequence":"additional","affiliation":[{"name":"Tianjin University of Science and Technology , , No 9, 13th, Avenue, Tianjin Economic and Technological Development Area , ,","place":["Tianjin, China, 300457"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7954-7700","authenticated-orcid":true,"given":"Weidong","family":"Liu","sequence":"additional","affiliation":[{"name":"Tianjin University of Science and Technology , , No 9, 13th, Avenue, Tianjin Economic and Technological Development Area , ,","place":["Tianjin, China, 300457"]},{"name":"Tianjin Institute of Industrial Biotechnology, Chinese Academy of Sciences , , ,","place":["Tianjin, China, 300308"]}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"316","published-online":{"date-parts":[[2026,9,2]]},"reference":[{"key":"2026091609084325800_cit1","doi-asserted-by":"publisher","first-page":"146511","DOI":"10.1016\/j.gene.2022.146511","article-title":"Oxidative stress as\na plausible mechanism for zearalenone to induce genome toxicity","volume":"829","author":"Feng","year":"2022","journal-title":"Gene"},{"issue":"24","key":"2026091609084325800_cit2","doi-asserted-by":"publisher","first-page":"5221","DOI":"10.1016\/j.scitotenv.2011.09.029","article-title":"Urinary mycoestrogens,\nbody size and breast development in New Jersey girls","volume":"409","author":"Bandera","year":"2011","journal-title":"Sci. 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