{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,21]],"date-time":"2026-10-21T13:49:50Z","timestamp":1792590590195,"version":"4.5.0"},"reference-count":46,"publisher":"American Chemical Society (ACS)","issue":"41","license":[{"start":{"date-parts":[[2026,10,9]],"date-time":"2026-10-09T00:00:00Z","timestamp":1791504000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"The State of Michigan ???PROJECT GREEEN???","award":["GR24-077"],"award-info":[{"award-number":["GR24-077"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2026,10,21]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>Indaziflam is a potent herbicide that has become an important tool for pre-emergence weed control. Although it is classified as a cellulose biosynthesis inhibitor, its precise site of action remains unresolved. Here, we show that indaziflam preferentially affects the root elongation zone, where cells exhibit swelling and frequent rupture. We performed ligand-baited coprecipitation assays using root tissue lysates from Arabidopsis thaliana and Oryza sativa. Proteomic analysis revealed that the highly enriched proteins were associated with vesicular trafficking, including multiple coatomer subunits, while no cellulose synthase components were detected. Live-cell imaging using FM4-64 dye demonstrated that indaziflam does not cause a complete inhibition of clathrin-mediated endocytosis. AlphaFold3-based protein\u2013ligand modeling further identified relatively stable predicted indaziflam poses with select trafficking-associated proteins, including coatomer subunit \u03b2. Collectively, our results support a model in which indaziflam indirectly disrupts cellulose biosynthesis by interfering with intracellular trafficking processes required for proper cellulose synthase complex homeostasis.<\/jats:p>","DOI":"10.1021\/acs.jafc.6c07779","type":"journal-article","created":{"date-parts":[[2026,10,9]],"date-time":"2026-10-09T11:02:39Z","timestamp":1791543759000},"page":"32184-32194","source":"Crossref","is-referenced-by-count":0,"title":["Ligand-Baited Proteomics\nReveals Vesicle Trafficking\nProteins as Putative Site of Action of Indaziflam"],"prefix":"10.1021","volume":"74","author":[{"ORCID":"https:\/\/orcid.org\/0009-0009-5479-7995","authenticated-orcid":true,"given":"Mohit","family":"Mahey","sequence":"first","affiliation":[{"name":"Michigan State University , , , ,","place":["East Lansing, Michigan, United States, 48824"]},{"name":"Michigan State University , , , ,","place":["East Lansing, Michigan, United States, 48824"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8390-8089","authenticated-orcid":true,"given":"Peter Knut","family":"Lundquist","sequence":"additional","affiliation":[{"name":"Michigan State University , , , ,","place":["East Lansing, Michigan, United States, 48824"]},{"name":"Michigan State University , , , ,","place":["East Lansing, Michigan, United States, 48824"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7111-6287","authenticated-orcid":true,"given":"Eric Lloyd","family":"Patterson","sequence":"additional","affiliation":[{"name":"Michigan State University , , , ,","place":["East Lansing, Michigan, United States, 48824"]}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"316","published-online":{"date-parts":[[2026,10,9]]},"reference":[{"issue":"2","key":"2026102109085489800_cit1","doi-asserted-by":"publisher","first-page":"384","DOI":"10.1017\/S0890037X00043116","article-title":"Classification of Herbicides by Site\nof Action for Weed Resistance Management Strategies","volume":"11","author":"Retzinger","year":"1997","journal-title":"Weed Technol."},{"issue":"3","key":"2026102109085489800_cit2","doi-asserted-by":"publisher","first-page":"1177","DOI":"10.1104\/pp.114.241950","article-title":"Indaziflam\nHerbicidal Action: A Potent Cellulose Biosynthesis Inhibitor1[C][W][OPEN]","volume":"166","author":"Brabham","year":"2014","journal-title":"Plant Physiol."},{"key":"2026102109085489800_cit3","doi-asserted-by":"crossref","first-page":"233","DOI":"10.1021\/bk-2015-1204.ch017","article-title":"Indaziflam: An\nInnovative Broad Spectrum Herbicide","volume-title":"Discovery\nand Synthesis of Crop Protection Products","author":"Ahrens","year":"2015"},{"issue":"1","key":"2026102109085489800_cit4","doi-asserted-by":"publisher","first-page":"539","DOI":"10.1002\/ps.70214","article-title":"Poa annua becomes\nthe first weed to evolve resistance to indaziflam applied preemergence\nand early-postemergence","volume":"82","author":"Miranda","year":"2026","journal-title":"Pest Manage. Sci."},{"issue":"6","key":"2026102109085489800_cit5","doi-asserted-by":"publisher","first-page":"2049","DOI":"10.1002\/ps.5740","article-title":"Annual bluegrass (Poa annua) resistance\nto indaziflam applied early-postemergence","volume":"76","author":"Brosnan","year":"2020","journal-title":"Pest\nManage. Sci."},{"issue":"1","key":"2026102109085489800_cit6","doi-asserted-by":"publisher","first-page":"278","DOI":"10.1104\/pp.126.1.278","article-title":"Temperature-sensitive\nalleles of RSW2 link the KORRIGAN endo-1,4-beta-glucanase to cellulose\nsynthesis and cytokinesis in Arabidopsis","volume":"126","author":"Lane","year":"2001","journal-title":"Plant\nPhysiol."},{"issue":"6","key":"2026102109085489800_cit7","doi-asserted-by":"publisher","first-page":"1749","DOI":"10.1105\/tpc.105.031732","article-title":"COBRA, an Arabidopsis\nextracellular glycosyl-phosphatidyl inositol-anchored protein, specifically\ncontrols highly anisotropic expansion through its involvement in cellulose\nmicrofibril orientation","volume":"17","author":"Roudier","year":"2005","journal-title":"Plant Cell"},{"issue":"29","key":"2026102109085489800_cit8","doi-asserted-by":"publisher","first-page":"12866","DOI":"10.1073\/pnas.1007092107","article-title":"Identification of a\ncellulose synthase-associated protein required for cellulose biosynthesis","volume":"107","author":"Gu","year":"2010","journal-title":"Proc. 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Sci."},{"issue":"2","key":"2026102109085489800_cit12","doi-asserted-by":"publisher","first-page":"482","DOI":"10.1104\/pp.010822","article-title":"Resistance against herbicide\nisoxaben and cellulose deficiency caused by distinct mutations in\nsame cellulose synthase isoform CESA6","volume":"128","author":"Desprez","year":"2002","journal-title":"Plant\nPhysiol."},{"issue":"1","key":"2026102109085489800_cit13","doi-asserted-by":"publisher","first-page":"146","DOI":"10.1104\/pp.90.1.146","article-title":"Mutation of a Locus\nof Arabidopsis thaliana Confers Resistance to the\nHerbicide Isoxaben","volume":"90","author":"Heim","year":"1989","journal-title":"Plant Physiol."},{"issue":"18","key":"2026102109085489800_cit14","doi-asserted-by":"publisher","first-page":"10079","DOI":"10.1073\/pnas.191361598","article-title":"Modifications\nof cellulose synthase confer resistance to isoxaben and thiazolidinone\nherbicides in Arabidopsis Ixr1 mutants","volume":"98","author":"Scheible","year":"2001","journal-title":"Proc.\nNatl. 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Plant Sci."},{"issue":"7","key":"2026102109085489800_cit34","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pgen.1006140","article-title":"Arabidopsis COG Complex Subunits COG3 and COG8Modulate\nGolgi Morphology, Vesicle Trafficking Homeostasis and Are Essential\nfor Pollen Tube Growth","volume":"12","author":"Tan","year":"2016","journal-title":"PLoS Genet."},{"key":"2026102109085489800_cit35","doi-asserted-by":"publisher","first-page":"315","DOI":"10.5511\/plantbiotechnology.25.315","article-title":"A survey of cellulose\nbiosynthesis in higher plants","volume":"25","author":"Bessueille","year":"2008","journal-title":"Plant Biotechnol."},{"key":"2026102109085489800_cit36","doi-asserted-by":"publisher","first-page":"245","DOI":"10.1146\/annurev.arplant.50.1.245","article-title":"CELLULOSE\nBIOSYNTHESIS: Exciting Times for A Difficult Field of Study","volume":"50","author":"Delmer","year":"1999","journal-title":"Annu. Rev. Plant Physiol Plant Mol. Biol."},{"issue":"4","key":"2026102109085489800_cit37","doi-asserted-by":"publisher","first-page":"1141","DOI":"10.1105\/tpc.108.065334","article-title":"Pausing of Golgi bodies\non microtubules regulates secretion of cellulose synthase complexes\nin Arabidopsis","volume":"21","author":"Crowell","year":"2009","journal-title":"Plant Cell"},{"issue":"7","key":"2026102109085489800_cit38","doi-asserted-by":"publisher","first-page":"797","DOI":"10.1038\/ncb1886","article-title":"Arabidopsis\ncortical microtubules position cellulose synthase delivery to the\nplasma membrane and interact with cellulose synthase trafficking compartments","volume":"11","author":"Gutierrez","year":"2009","journal-title":"Nat. Cell Biol."},{"issue":"1","key":"2026102109085489800_cit39","doi-asserted-by":"publisher","first-page":"150","DOI":"10.1104\/pp.113.221234","article-title":"The endocytosis of\ncellulose synthase in Arabidopsis is dependent on \u03bc2, a clathrin-mediated\nendocytosis adaptin","volume":"163","author":"Bashline","year":"2013","journal-title":"Plant Physiol."},{"issue":"41","key":"2026102109085489800_cit40","doi-asserted-by":"publisher","first-page":"12870","DOI":"10.1073\/pnas.1509292112","article-title":"The TWD40\u20132 protein and the\nAP2 complex cooperate in the clathrin-mediated endocytosis of cellulose\nsynthase to regulate cellulose biosynthesis","volume":"112","author":"Bashline","year":"2015","journal-title":"Proc. Natl. Acad. Sci. U.S.A."},{"issue":"8","key":"2026102109085489800_cit41","doi-asserted-by":"publisher","first-page":"2666","DOI":"10.1529\/biophysj.106.099473","article-title":"The cellulose synthase\ncomplex: a polymerization driven\nsupramolecular motor","volume":"92","author":"Diotallevi","year":"2007","journal-title":"Biophys. J."},{"issue":"1","key":"2026102109085489800_cit42","doi-asserted-by":"publisher","first-page":"132","DOI":"10.1105\/tpc.104.026351","article-title":"Receptor salvage from\nthe prevacuolar compartment is essential for efficient vacuolar protein\ntargeting","volume":"17","author":"dasilva","year":"2005","journal-title":"Plant Cell"},{"issue":"11","key":"2026102109085489800_cit43","doi-asserted-by":"publisher","first-page":"1051","DOI":"10.1111\/jipb.12694","article-title":"Rab-H1b is essential for trafficking of cellulose\nsynthase and for hypocotyl growth in Arabidopsis thaliana","volume":"60","author":"He","year":"2018","journal-title":"J. Integr. 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Plant\nSci."},{"issue":"2","key":"2026102109085489800_cit46","doi-asserted-by":"publisher","first-page":"701","DOI":"10.1104\/pp.105.059774","article-title":"Loss-of-function\nmutations of ROOT HAIR DEFECTIVE3 suppress root waving, skewing, and\nepidermal cell file rotation in Arabidopsis","volume":"138","author":"Yuen","year":"2005","journal-title":"Plant Physiol."}],"container-title":["Journal of Agricultural\nand Food Chemistry"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/pubs.acs.org\/doi\/pdf\/10.1021\/acs.jafc.6c07779","content-type":"application\/pdf","content-version":"vor","intended-application":"unspecified"},{"URL":"https:\/\/pubs.acs.org\/jafcau\/article-pdf\/74\/41\/32184\/68797231\/acs.jafc.6c07779.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"syndication"},{"URL":"https:\/\/pubs.acs.org\/jafcau\/article-pdf\/74\/41\/32184\/68797231\/acs.jafc.6c07779.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,10,21]],"date-time":"2026-10-21T13:29:46Z","timestamp":1792589386000},"score":1,"resource":{"primary":{"URL":"https:\/\/pubs.acs.org\/jafcau\/article\/74\/41\/32184\/5447622\/Ligand-Baited-Proteomics-Reveals-Vesicle"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,10,9]]},"references-count":46,"journal-issue":{"issue":"41","published-online":{"date-parts":[[2026,10,9]]},"published-print":{"date-parts":[[2026,10,21]]}},"URL":"https:\/\/doi.org\/10.1021\/acs.jafc.6c07779","relation":{},"ISSN":["0021-8561","1520-5118"],"issn-type":[{"value":"0021-8561","type":"print"},{"value":"1520-5118","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026,10,9]]}}}