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Here, we developed a glucose\u2013glycerol cofermentation strategy to improve laccase production in Aureobasidium melanogenum ZN by regulating cellular morphology and metabolism. The results showed that glycerol stabilized yeast-like growth and suppressed melanized differentiation, while the optimized combination of 2% glucose and 7.5% glycerol increased laccase activity and yield to 418.43 U\u00b7mL\u20131 and 65.67 U\u00b7g\u20131 dry weight (DW), respectively. The crude laccase showed an optimal activity at 75 \u00b0C and efficiently decolorized triphenylmethane dyes, especially malachite green, with partial activity retained under NaCl-containing and simulated wastewater-like conditions. Physiological and multiomics analyses linked glycerol-enhanced laccase production to yeast-like-state maintenance, carbon-source regulation, respiratory metabolism, redox adjustment, antioxidant responses, and reduced melanized differentiation. This study provides a morphology\u2013metabolism-directed strategy for enhancing laccase production in native polymorphic fungi.<\/jats:p>","DOI":"10.1021\/acs.jafc.6c07800","type":"journal-article","created":{"date-parts":[[2026,8,7]],"date-time":"2026-08-07T12:01:32Z","timestamp":1786104092000},"page":"25490-25506","source":"Crossref","is-referenced-by-count":0,"title":["Glycerol Stabilizes\nYeast-like Morphology and Promotes\nLaccase Biosynthesis through Metabolic Remodeling in\n                    <i>Aureobasidium melanogenum<\/i>\n                    ZN"],"prefix":"10.1021","volume":"74","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-9584-3902","authenticated-orcid":true,"given":"Nan","family":"Zeng","sequence":"first","affiliation":[{"name":"Shenyang Agricultural University , , ,","place":["Shenyang, P. R. 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