{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,29]],"date-time":"2026-07-29T15:04:33Z","timestamp":1785337473667,"version":"3.55.0"},"reference-count":42,"publisher":"American Chemical Society (ACS)","issue":"29","license":[{"start":{"date-parts":[[2026,7,14]],"date-time":"2026-07-14T00:00:00Z","timestamp":1783987200000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-029"},{"start":{"date-parts":[[2026,7,14]],"date-time":"2026-07-14T00:00:00Z","timestamp":1783987200000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-037"},{"start":{"date-parts":[[2026,7,14]],"date-time":"2026-07-14T00:00:00Z","timestamp":1783987200000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-045"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["32472449"],"award-info":[{"award-number":["32472449"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["U23A20266"],"award-info":[{"award-number":["U23A20266"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2026,7,29]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>Filamin C is an important allergen in Scylla paramamosain and designated as Scy p 9. Hypoallergenic derivatives are one of the tools for solving specific allergy problems. In this study, molecular modification techniques were used to delete the N-\/C-terminal region (AA1\u223c10, AA839\u223c848), mutate the key amino acids (T270), and delete the linear B-cell epitopes of (L-AA187\u223c205, L-AA363\u223c377) of Scy p 9, thereby generating the derivative Scy p 9NCCL. Compared with Scy p 9, Scy p 9NCCL exhibited weaker immune binding activity and markedly attenuated its capacity to trigger basophil activation, indicating that Scy p 9NCCL represents a hypoallergenic derivative. In vivo, Scy p 9NCCL induced significantly lower levels of mouse-specific IgE, whereas the levels of Treg-cell differentiation were significantly higher. Scy p 9NCCL induced production of specific blocking antibodies and alleviated allergic reactions caused by Scy p 9. These results laid a foundation for alleviating seafood allergies.<\/jats:p>","DOI":"10.1021\/acs.jafc.6c07949","type":"journal-article","created":{"date-parts":[[2026,7,15]],"date-time":"2026-07-15T01:38:53Z","timestamp":1784079533000},"page":"23115-23129","source":"Crossref","is-referenced-by-count":0,"title":["Hypoallergenic\nDerivatives of Scy p 9 for Alleviating\nSpecific Allergic Reactions in Mice"],"prefix":"10.1021","volume":"74","author":[{"given":"Xin-Rong","family":"He","sequence":"first","affiliation":[{"name":"The First Affiliated Hospital of Xiamen University , , , ,","place":["Xiamen, Fujian, China, 361000"]},{"name":"Jimei University , , , ,","place":["Xiamen, Fujian, China, 361021"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1083-5418","authenticated-orcid":true,"given":"Yang","family":"Yang","sequence":"additional","affiliation":[{"name":"Jimei University , , , ,","place":["Xiamen, Fujian, China, 361021"]},{"name":"Xiamen Huaxia University , , 288 Tianma Road , , ,","place":["Xiamen, Fujian, China, 361024"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Fei","family":"Huan","sequence":"additional","affiliation":[{"name":"Jimei University , , , ,","place":["Xiamen, Fujian, China, 361021"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xin-Yu","family":"Han","sequence":"additional","affiliation":[{"name":"Jimei University , , , ,","place":["Xiamen, Fujian, China, 361021"]},{"name":"Xiamen Ocean Vocational College , , , ,","place":["Xiamen, Fujian, China, 361100"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Fa-Jie","family":"Li","sequence":"additional","affiliation":[{"name":"Jimei University , , , ,","place":["Xiamen, Fujian, China, 361021"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Gui-Xia","family":"Chen","sequence":"additional","affiliation":[{"name":"Women and Children\u2019s Hospital Affiliated of Xiamen University , , ,","place":["Xiamen, Fujian, China, 361000"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1875-5869","authenticated-orcid":true,"given":"Xiao-Mei","family":"Chen","sequence":"additional","affiliation":[{"name":"Jimei University , , , ,","place":["Xiamen, Fujian, China, 361021"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xue-Qin","family":"Chen","sequence":"additional","affiliation":[{"name":"The First Affiliated Hospital of Xiamen University , , , ,","place":["Xiamen, Fujian, China, 361000"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8689-0504","authenticated-orcid":true,"given":"Guang-Ming","family":"Liu","sequence":"additional","affiliation":[{"name":"Jimei University , , , ,","place":["Xiamen, Fujian, China, 361021"]},{"name":"Xiamen Ocean Vocational College , , , ,","place":["Xiamen, Fujian, China, 361100"]}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"316","published-online":{"date-parts":[[2026,7,14]]},"reference":[{"issue":"5","key":"2026072910074581000_cit1","doi-asserted-by":"publisher","first-page":"S54","DOI":"10.1016\/j.anai.2018.09.174","article-title":"Egg allergy in us children","volume":"121","author":"Samady","year":"2018","journal-title":"Ann. Allerg. Asthma\nIm."},{"issue":"2","key":"2026072910074581000_cit2","doi-asserted-by":"publisher","first-page":"690","DOI":"10.1016\/j.jaip.2018.09.011","article-title":"Low resolution rates of seafood allergy","volume":"7","author":"Ben Shoshan","year":"2019","journal-title":"J. Allergy Clin Immunol Pract."},{"key":"2026072910074581000_cit3","volume-title":"China Fishery Statistical\nYearbook","author":"Liu","year":"2025"},{"issue":"1","key":"2026072910074581000_cit4","doi-asserted-by":"publisher","first-page":"108","DOI":"10.1111\/cea.13266","article-title":"A comprehensive\nanalysis of the allergenicity and IgE\nepitopes of myosinogen allergens in Scylla paramamosain","volume":"49","author":"Yang","year":"2019","journal-title":"Clin. Exp. Allergy"},{"key":"2026072910074581000_cit5","doi-asserted-by":"publisher","first-page":"131588","DOI":"10.1016\/j.foodchem.2021.131588","article-title":"Two hypo-allergenic\nderivatives lacking the dominant linear epitope of Scy p 1 and Scy\np 3","volume":"373","author":"Li","year":"2022","journal-title":"Food Chem."},{"issue":"4","key":"2026072910074581000_cit6","doi-asserted-by":"publisher","first-page":"3006","DOI":"10.1039\/D0FO00004C","article-title":"Cloning, expression and comparison\nof the properties\nof Scy p 9, a Scylla paramamosain allergen","volume":"11","author":"He","year":"2020","journal-title":"Food Funct."},{"key":"2026072910074581000_cit7","doi-asserted-by":"publisher","first-page":"1282","DOI":"10.1021\/acs.jafc.1c07922","article-title":"Crystal structure analysis and IgE epitope mapping\nof allergic predominant region in Scylla paramamosain filamin c","volume":"70","author":"He","year":"2022","journal-title":"J. Agr. Food Chem."},{"key":"2026072910074581000_cit8","doi-asserted-by":"publisher","first-page":"104248","DOI":"10.1016\/j.tifs.2023.104248","article-title":"Effect of enzymatic\nhydrolysis combined with processing\non allergenicity of food allergens","volume":"143","author":"Pang","year":"2024","journal-title":"Trends Food\nSci. Technol."},{"issue":"24","key":"2026072910074581000_cit9","doi-asserted-by":"publisher","first-page":"15321","DOI":"10.1021\/acs.jafc.5c02975","article-title":"Impact\nof heat treatment on the allergenic potential of penaeus chinensis\nTM: A comprehensive analysis of structural changes, molecular dynamics,\ndigestibility, allergenicity and gut microbiota alterations","volume":"73","author":"Liu","year":"2025","journal-title":"J. Agric. Food Chem."},{"key":"2026072910074581000_cit10","doi-asserted-by":"publisher","first-page":"744544","DOI":"10.3389\/fimmu.2021.744544","article-title":"Preventive Administration of Non-Allergenic\nBet v 1 Peptides Reduces Allergic Sensitization to Major Birch Pollen\nAllergen, Bet v 1","volume":"12","author":"Akinfenwa","year":"2021","journal-title":"Front. Immunol."},{"key":"2026072910074581000_cit11","doi-asserted-by":"publisher","first-page":"1250","DOI":"10.1038\/s42256-025-01073-z","article-title":"Self-iterative\nmultiple-instance learning enables the prediction of CD4+ T cell immunogenic epitopes","volume":"7","author":"Shen","year":"2025","journal-title":"Nat. Mach. Intell."},{"issue":"8","key":"2026072910074581000_cit12","doi-asserted-by":"publisher","first-page":"4936","DOI":"10.1021\/acs.jafc.4c11853","article-title":"Study on the Allergenicity\nof Tropomyosin from Different\nAquatic Products Based on Conformational and Linear Epitopes Analysis","volume":"73","author":"Lv","year":"2025","journal-title":"J. Agr. Food Chem."},{"key":"2026072910074581000_cit13","doi-asserted-by":"publisher","first-page":"115512","DOI":"10.1016\/j.fct.2025.115512","article-title":"Non-allergenic mixed T cell epitope\npeptides for preventing\nScy p 9 allergic reactions","volume":"202","author":"He","year":"2025","journal-title":"Food Chem. Toxicol."},{"issue":"45","key":"2026072910074581000_cit14","doi-asserted-by":"publisher","first-page":"17379","DOI":"10.1021\/acs.jafc.3c04970","article-title":"Immunoglobulin E epitope mapping\nand structure-allergenicity\nrelationship analysis of crab allergen Scy p 9","volume":"71","author":"He","year":"2023","journal-title":"J. Agr. Food Chem."},{"issue":"4","key":"2026072910074581000_cit15","doi-asserted-by":"publisher","first-page":"793","DOI":"10.1111\/all.16027","article-title":"Evaluation of clinical outcomes of\nefficacy in food allergen immunotherapy trials, COFAITH EAACI task\nforce","volume":"79","author":"Ri\u0301o","year":"2024","journal-title":"Allergy"},{"key":"2026072910074581000_cit16","doi-asserted-by":"publisher","first-page":"760","DOI":"10.1038\/s41586-024-08440-7","article-title":"RELM\u03b2 sets the threshold for microbiome-dependent\noral tolerance","volume":"638","author":"Stephen-Victor","year":"2025","journal-title":"Nature"},{"issue":"4","key":"2026072910074581000_cit17","doi-asserted-by":"publisher","first-page":"920","DOI":"10.1016\/j.jaci.2022.04.031","article-title":"Art v 1\nIgE epitopes of patients and humanized mice are conformational","volume":"150","author":"Zabel","year":"2022","journal-title":"J. Allergy Clin. Immun."},{"issue":"8","key":"2026072910074581000_cit18","doi-asserted-by":"publisher","first-page":"2555","DOI":"10.1111\/all.14817","article-title":"IgE-cross-blocking antibodies to Fagales following sublingual immunotherapy\nwith recombinant Bet v 1","volume":"76","author":"Grilo","year":"2021","journal-title":"Allergy"},{"issue":"5","key":"2026072910074581000_cit19","doi-asserted-by":"publisher","first-page":"1371","DOI":"10.1016\/j.jaci.2023.01.005","article-title":"The role of IgG1 and\nIgG4 as dominant IgE-blocking antibodies shifts during allergen immunotherapy","volume":"151","author":"Strobl","year":"2023","journal-title":"J. Allergy Clin. Immun."},{"key":"2026072910074581000_cit20","doi-asserted-by":"publisher","first-page":"1973","DOI":"10.1016\/j.ijbiomac.2020.07.325","article-title":"Hypoallergenic\nmutants of the major oyster allergen Cra g 1 alleviate oyster tropomyosin\nallergenic potency","volume":"164","author":"Zhang","year":"2020","journal-title":"Int. J. Biol. Macromol."},{"issue":"16","key":"2026072910074581000_cit21","doi-asserted-by":"publisher","first-page":"9389","DOI":"10.1021\/acs.jafc.4c01120","article-title":"Mutation of disulfide bond sites\nreduces the immunoreactivity\nof Cra a 4 by changing the structural characteristics","volume":"72","author":"Gao","year":"2024","journal-title":"J. Agr. Food Chem."},{"key":"2026072910074581000_cit22","doi-asserted-by":"publisher","first-page":"108601","DOI":"10.1016\/j.intimp.2022.108601","article-title":"Molecular\nand immunochemical characterization of profilin as major allergen\nfrom platanus acerifolia pollen","volume":"106","author":"Yang","year":"2022","journal-title":"Int. Immunopharmacol."},{"key":"2026072910074581000_cit23","doi-asserted-by":"publisher","first-page":"136240","DOI":"10.1016\/j.foodchem.2023.136240","article-title":"Physicochemical\ncharacterization of changes in pea protein as the result of cold extrusion","volume":"423","author":"Helmick","year":"2023","journal-title":"Food Chem."},{"issue":"18","key":"2026072910074581000_cit24","doi-asserted-by":"publisher","first-page":"5221","DOI":"10.1021\/acs.jafc.0c01543","article-title":"Identification and\ncross-reactivity analysis of sarcoplasmic calcium-binding protein\na novel allergen in Crassostrea angulata","volume":"68","author":"Han","year":"2020","journal-title":"J. Agr. Food Chem."},{"issue":"10","key":"2026072910074581000_cit25","doi-asserted-by":"publisher","first-page":"2924","DOI":"10.1111\/all.15278","article-title":"Do basophil activation tests help\nelucidate allergic\nreactions to the ingredients in COVID-19 vaccines?","volume":"77","author":"Eberlein","year":"2022","journal-title":"Allergy"},{"issue":"10","key":"2026072910074581000_cit26","doi-asserted-by":"publisher","first-page":"1900496","DOI":"10.1002\/mnfr.201900496","article-title":"Lactobacillus casei\nzhang alleviates shrimp Tropomyosin-Induced food allergy by switching\nantibody isotypes through the NF-\u03baB-Dependent immune tolerance","volume":"64","author":"Fu","year":"2020","journal-title":"Mol. Nutr. Food Res."},{"issue":"3","key":"2026072910074581000_cit27","doi-asserted-by":"publisher","first-page":"292","DOI":"10.1093\/cei\/uxac033","article-title":"Per a 5-derived T-cell peptides modulate\nNF-kB signalling to ameliorate allergic inflammation systemically\nin murine model of cockroach allergic hyper-reactivity","volume":"208","author":"Sharma","year":"2022","journal-title":"Clin. Exp. Immunol."},{"key":"2026072910074581000_cit28","doi-asserted-by":"publisher","first-page":"1001","DOI":"10.1111\/all.15919","article-title":"Recombinant\nPreS-fusion protein vaccine for birch pollen and apple allergy","volume":"0","author":"Khaitov","year":"2023","journal-title":"Allergy"},{"issue":"6","key":"2026072910074581000_cit29","doi-asserted-by":"publisher","first-page":"1611","DOI":"10.1016\/j.jaci.2024.02.017","article-title":"Neutralizing IgG4\nantibodies are a biomarker of sustained\nefficacy after peanut oral immunotherapy","volume":"153","author":"Keswani","year":"2024","journal-title":"J.\nAllergy Clin. Immun."},{"issue":"1","key":"2026072910074581000_cit30","doi-asserted-by":"publisher","first-page":"182","DOI":"10.1016\/j.jaci.2022.07.011","article-title":"IL-4 receptor\nalpha signaling alters oral food challenge and immunotherapy outcomes\nin mice","volume":"151","author":"Ganesan","year":"2023","journal-title":"J. Allergy Clin. Immun."},{"issue":"8","key":"2026072910074581000_cit31","doi-asserted-by":"publisher","first-page":"1107","DOI":"10.1111\/cea.13432","article-title":"Variable IgE cross-reactivity between\npeanut 2S-albumins: The case for measuring IgE to both Ara h 2 and\nAra h 6","volume":"49","author":"Hazebrouck","year":"2019","journal-title":"Clin. Exp. Allergy"},{"issue":"24","key":"2026072910074581000_cit32","doi-asserted-by":"publisher","first-page":"9508","DOI":"10.1021\/acs.jafc.3c01549","article-title":"IgE epitope analysis\nand hypo-immunoreactivity derivative of arginine kinase in mantis\nshrimp (Oratosquilla oratoria)","volume":"71","author":"Chen","year":"2023","journal-title":"J. Agr. Food Chem."},{"issue":"2","key":"2026072910074581000_cit33","doi-asserted-by":"publisher","first-page":"468","DOI":"10.1016\/j.jaci.2025.04.010","article-title":"Baseline basophil activation and\nearly suppression\nis associated with clinical outcome after peanut sublingual immunotherapy","volume":"156","author":"Humphrey","year":"2025","journal-title":"J. Allergy Clin. Immun."},{"issue":"1","key":"2026072910074581000_cit34","doi-asserted-by":"publisher","first-page":"229","DOI":"10.1016\/j.jaci.2019.08.036","article-title":"Development\nof a novel Ara h 2 hypoallergen with no\nIgE binding or anaphylactogenic activity","volume":"145","author":"Tscheppe","year":"2020","journal-title":"J.\nAllergy Clin. Immun."},{"key":"2026072910074581000_cit35","doi-asserted-by":"publisher","first-page":"401","DOI":"10.1146\/annurev-immunol-090122-043501","article-title":"The immunobiology and\ntreatment of\nfood allergy","volume":"42","author":"Barshow","year":"2024","journal-title":"Annu. Rev. Immunol."},{"issue":"7","key":"2026072910074581000_cit36","doi-asserted-by":"publisher","first-page":"1995","DOI":"10.1111\/all.16530","article-title":"Vaccine-Induced\nAnti-IgE antibodies neutralize free IgE but fail to bind and activate\nmast Cell-Displayed IgE","volume":"80","author":"Gharailoo","year":"2025","journal-title":"Allergy"},{"issue":"4","key":"2026072910074581000_cit37","doi-asserted-by":"publisher","first-page":"923","DOI":"10.1111\/all.15631","article-title":"Introduction of General\nChapters on standard methods for allergen quantification in the European\nPharmacopoeia","volume":"78","author":"Zimmer","year":"2023","journal-title":"Allergy"},{"issue":"6","key":"2026072910074581000_cit38","doi-asserted-by":"publisher","first-page":"1605","DOI":"10.1111\/all.15659","article-title":"Targeting Ara h 2 with human-derived\nmonoclonal antibodies prevents peanut-induced anaphylaxis","volume":"78","author":"Paolucci","year":"2023","journal-title":"Allergy"},{"issue":"682","key":"2026072910074581000_cit39","doi-asserted-by":"publisher","first-page":"6373","DOI":"10.1126\/scitranslmed.add6373","article-title":"Peanut allergen inhibition prevents\nanaphylaxis in a humanized mouse model","volume":"15","author":"Alakhras","year":"2023","journal-title":"Sci.\nTransl. Med."},{"issue":"2","key":"2026072910074581000_cit40","doi-asserted-by":"publisher","first-page":"425","DOI":"10.1016\/j.jaci.2022.02.024","article-title":"A mouse model of the\nLEAP study reveals\na role for CTLA-4 in preventing peanut allergy induced by environmental\npeanut exposure","volume":"150","author":"Krempski","year":"2022","journal-title":"J. Allergy Clin. Immun."},{"key":"2026072910074581000_cit41","doi-asserted-by":"publisher","first-page":"2089","DOI":"10.1111\/all.70111","article-title":"Peanut Component-Specific\nIgE and IgG4 are modulated by sublingual\nimmunotherapy and are associated with remission in 1\u20134-Year-Old\nchildren","volume":"81","author":"Singh","year":"2025","journal-title":"Allergy"},{"issue":"794","key":"2026072910074581000_cit42","doi-asserted-by":"publisher","first-page":"1","DOI":"10.1126\/scitranslmed.adl0406","article-title":"Liver-targeted allergen immunotherapy\nrapidly and safely induces antigen-specific tolerance to treat allergic\nairway disease in mice","volume":"17","author":"Medelli\u0301n","year":"2025","journal-title":"Sci. Transl. Med."}],"container-title":["Journal of Agricultural\nand Food Chemistry"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/pubs.acs.org\/doi\/pdf\/10.1021\/acs.jafc.6c07949","content-type":"application\/pdf","content-version":"vor","intended-application":"unspecified"},{"URL":"https:\/\/pubs.acs.org\/jafcau\/article-pdf\/74\/29\/23115\/65946012\/acs.jafc.6c07949.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"syndication"},{"URL":"https:\/\/pubs.acs.org\/jafcau\/article-pdf\/74\/29\/23115\/65946012\/acs.jafc.6c07949.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,7,29]],"date-time":"2026-07-29T14:19:59Z","timestamp":1785334799000},"score":1,"resource":{"primary":{"URL":"https:\/\/pubs.acs.org\/jafcau\/article\/74\/29\/23115\/5210164\/Hypoallergenic-Derivatives-of-Scy-p-9-for"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,7,14]]},"references-count":42,"journal-issue":{"issue":"29","published-online":{"date-parts":[[2026,7,14]]},"published-print":{"date-parts":[[2026,7,29]]}},"URL":"https:\/\/doi.org\/10.1021\/acs.jafc.6c07949","relation":{},"ISSN":["0021-8561","1520-5118"],"issn-type":[{"value":"0021-8561","type":"print"},{"value":"1520-5118","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026,7,14]]}}}