{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,1]],"date-time":"2026-09-01T09:18:07Z","timestamp":1788254287970,"version":"build-2803163510"},"reference-count":24,"publisher":"American Chemical Society (ACS)","issue":"33","license":[{"start":{"date-parts":[[2026,8,11]],"date-time":"2026-08-11T00:00:00Z","timestamp":1786406400000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-029"},{"start":{"date-parts":[[2026,8,11]],"date-time":"2026-08-11T00:00:00Z","timestamp":1786406400000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-037"},{"start":{"date-parts":[[2026,8,11]],"date-time":"2026-08-11T00:00:00Z","timestamp":1786406400000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-045"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["U24A20809"],"award-info":[{"award-number":["U24A20809"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100002858","name":"China Postdoctoral Science Foundation","doi-asserted-by":"publisher","award":["2025M770856"],"award-info":[{"award-number":["2025M770856"]}],"id":[{"id":"10.13039\/501100002858","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100002858","name":"China Postdoctoral Science Foundation","doi-asserted-by":"publisher","award":["GZC20250581"],"award-info":[{"award-number":["GZC20250581"]}],"id":[{"id":"10.13039\/501100002858","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100012166","name":"National Key Research and Development Program of China","doi-asserted-by":"publisher","award":["2024YFC2815900"],"award-info":[{"award-number":["2024YFC2815900"]}],"id":[{"id":"10.13039\/501100012166","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100021171","name":"Basic and Applied Basic Research Foundation of Guangdong Province","doi-asserted-by":"publisher","award":["2026A1515010947"],"award-info":[{"award-number":["2026A1515010947"]}],"id":[{"id":"10.13039\/501100021171","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2026,8,26]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>Piericidins are \u03b1-pyridone agricultural antibiotics produced by actinomycetes. To improve their yield and structural diversity from marine-derived Streptomyces psammoticus SCSIO NS126, this study employed homologous recombination to achieve large-fragment knockout of modification genes within the piericidin biosynthetic gene cluster. Deletion of the pie56\u201365 genes markedly elevated piericidin titers. Large-scale culture of the mutant afforded 21 piericidins, including 4 new analogues (1\u20134), and their plausible biosynthetic pathway was proposed. All isolated compounds displayed broad-spectrum antimicrobial effects, while compounds 4 and 5 showed potent activity against Staphylococcus aureus (minimum inhibitory concentration (MIC) = 0.391 \u03bcg\/mL) and Alternaria solani (MIC = 0.781 \u03bcg\/mL), respectively. Mechanistically, active piericidins damage pathogen membrane integrity, and compound 19 exhibited dose-dependent in vivo antifungal efficacy in plant infection assays. This study expands piericidin profiling and provides abundant candidate molecules for the development of new eco-friendly agricultural antibiotics.<\/jats:p>","DOI":"10.1021\/acs.jafc.6c08189","type":"journal-article","created":{"date-parts":[[2026,8,12]],"date-time":"2026-08-12T03:02:08Z","timestamp":1786503728000},"page":"26417-26426","source":"Crossref","is-referenced-by-count":0,"title":["Modification Gene\nKnockout Diversifies Piericidins\nfrom the Marine-Derived\n                    <i>Streptomyces psammoticus<\/i>\n                    SCSIO NS126"],"prefix":"10.1021","volume":"74","author":[{"given":"Jiaqi","family":"Liang","sequence":"first","affiliation":[{"name":"Chinese Academy of Sciences , , ,","place":["Guangzhou, China, 510301"]},{"name":"University of Chinese Academy of Sciences , ,","place":["Beijing, China, 100049"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jian","family":"Cai","sequence":"additional","affiliation":[{"name":"Chinese Academy of Sciences , , ,","place":["Guangzhou, China, 510301"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mengqin","family":"Yu","sequence":"additional","affiliation":[{"name":"Chinese Academy of Sciences , , ,","place":["Guangzhou, China, 510301"]},{"name":"Guangxi University of Chinese Medicine , , ,","place":["Nanning, China, 530200"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rongxiang","family":"Qiu","sequence":"additional","affiliation":[{"name":"Southern Medical University , , ,","place":["Guangzhou, China, 510515"]},{"name":"Guangdong Pharmaceutical University , , ,","place":["Guangzhou, China, 510006"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yuecheng","family":"Fang","sequence":"additional","affiliation":[{"name":"Chinese Academy of Sciences , , ,","place":["Guangzhou, China, 510301"]},{"name":"University of Chinese Academy of Sciences , ,","place":["Beijing, China, 100049"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8327-3108","authenticated-orcid":true,"given":"Yonghong","family":"Liu","sequence":"additional","affiliation":[{"name":"Chinese Academy of Sciences , , ,","place":["Guangzhou, China, 510301"]},{"name":"University of Chinese Academy of Sciences , ,","place":["Beijing, China, 100049"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2345-0886","authenticated-orcid":true,"given":"Lan","family":"Tang","sequence":"additional","affiliation":[{"name":"Southern Medical University , , ,","place":["Guangzhou, China, 510515"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9601-4869","authenticated-orcid":true,"given":"Xuefeng","family":"Zhou","sequence":"additional","affiliation":[{"name":"Chinese Academy of Sciences , , ,","place":["Guangzhou, China, 510301"]},{"name":"University of Chinese Academy of Sciences , ,","place":["Beijing, China, 100049"]}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"316","published-online":{"date-parts":[[2026,8,11]]},"reference":[{"key":"2026090104464061200_cit1","doi-asserted-by":"publisher","first-page":"87","DOI":"10.3390\/md14050087","article-title":"Bioprospecting sponge-associated\nmicrobes for antimicrobial compounds","volume":"14","author":"Indraningrat","year":"2016","journal-title":"Mar. 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