{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,30]],"date-time":"2026-09-30T14:00:33Z","timestamp":1790776833927,"version":"4.1.0"},"reference-count":36,"publisher":"American Chemical Society (ACS)","issue":"38","license":[{"start":{"date-parts":[[2026,9,18]],"date-time":"2026-09-18T00:00:00Z","timestamp":1789689600000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-029"},{"start":{"date-parts":[[2026,9,18]],"date-time":"2026-09-18T00:00:00Z","timestamp":1789689600000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-037"},{"start":{"date-parts":[[2026,9,18]],"date-time":"2026-09-18T00:00:00Z","timestamp":1789689600000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-045"}],"funder":[{"DOI":"10.13039\/501100005196","name":"Chinese Academy of Agricultural Sciences","doi-asserted-by":"publisher","award":["CAASCSCB202401"],"award-info":[{"award-number":["CAASCSCB202401"]}],"id":[{"id":"10.13039\/501100005196","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100005045","name":"Ministry of Finance","doi-asserted-by":"publisher","award":["Y2025YC19"],"award-info":[{"award-number":["Y2025YC19"]}],"id":[{"id":"10.13039\/501100005045","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["22177043"],"award-info":[{"award-number":["22177043"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["22377045"],"award-info":[{"award-number":["22377045"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2026,9,30]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>Xenocoumacin 1 (Xcn1), a dihydroisocoumarin-derived antimicrobial natural product from Xenorhabdus nematophila CB6-T2, exhibits broad-spectrum antifungal activity, yet its antibacterial mechanism remains unknown. This study evaluated the antibacterial spectrum of Xcn1 against 13 bacterial strains, with Ralstonia solanacearum K60-1 showing the highest susceptibility (MIC90 = 0.780 \u03bcg\/mL). In pot experiments, Xcn1 provided &amp;gt;66.0% efficacy against bacterial wilt, comparable to zhongshengmycin. Phenotypic assays revealed antibacterial effects characterized by membrane disruption, together with antivirulence effects, including inhibition of biofilm formation (90.3%) and suppression of swimming and twitching motilities (&amp;gt;61.0%). Integrated transcriptomic and proteomic analyses identified 934 differentially expressed genes and 356 differentially expressed proteins, highlighting oxidative phosphorylation as the most enriched pathway and ubiB and glmU as key downregulated hub genes. These findings reveal a multi-target antibacterial mechanism involving membrane disruption, energy metabolism collapse, and virulence attenuation, positioning Xcn1 as a promising green antibacterial candidate for the management of bacterial wilt. Future studies should focus on formulation optimization, field-scale efficacy and long-term resistance monitoring, and further confirmation of its direct molecular targets.<\/jats:p>","DOI":"10.1021\/acs.jafc.6c08360","type":"journal-article","created":{"date-parts":[[2026,9,18]],"date-time":"2026-09-18T10:01:19Z","timestamp":1789725679000},"page":"30068-30078","source":"Crossref","is-referenced-by-count":0,"title":["Xenocoumacin 1\nfrom\n                    <i>Xenorhabdus nematophila<\/i>\n                    CB6-T2:\nAntibacterial Spectrum and Multi-Target Mode of Action against\n                    <i>Ralstonia solanacearum<\/i>\n                    K60-1"],"prefix":"10.1021","volume":"74","author":[{"given":"Liu","family":"Yang","sequence":"first","affiliation":[{"name":"Lanzhou University , , ,","place":["Lanzhou, People\u2019s Republic of China, 730000"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ya-Ya","family":"Lei","sequence":"additional","affiliation":[{"name":"Lanzhou University , , ,","place":["Lanzhou, People\u2019s Republic of China, 730000"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jin-Cheng","family":"Ma","sequence":"additional","affiliation":[{"name":"Lanzhou University , , ,","place":["Lanzhou, People\u2019s Republic of China, 730000"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hui-Wen","family":"Wang","sequence":"additional","affiliation":[{"name":"Lanzhou University , , ,","place":["Lanzhou, People\u2019s Republic of China, 730000"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yu-Chen","family":"Sun","sequence":"additional","affiliation":[{"name":"Lanzhou University , , ,","place":["Lanzhou, People\u2019s Republic of China, 730000"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Bao-Qi","family":"Zhang","sequence":"additional","affiliation":[{"name":"Lanzhou University , , ,","place":["Lanzhou, People\u2019s Republic of China, 730000"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Guo-Liang","family":"Mou","sequence":"additional","affiliation":[{"name":"Lanzhou University , , ,","place":["Lanzhou, People\u2019s Republic of China, 730000"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shao-Yong","family":"Zhang","sequence":"additional","affiliation":[{"name":"Huzhou University , , ,","place":["Huzhou, China, 313000"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3055-3426","authenticated-orcid":true,"given":"Zhi-Jun","family":"Zhang","sequence":"additional","affiliation":[{"name":"Lanzhou University , , ,","place":["Lanzhou, People\u2019s Republic of China, 730000"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ji","family":"Xie","sequence":"additional","affiliation":[{"name":"Institute of Plant Protection Chinese Academy of Agricultural Sciences , , ,","place":["Beijing, China, 100193"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Guang-yue","family":"Li","sequence":"additional","affiliation":[{"name":"Institute of Plant Protection Chinese Academy of Agricultural Sciences , , ,","place":["Beijing, China, 100193"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5600-0913","authenticated-orcid":true,"given":"Ying-Qian","family":"Liu","sequence":"additional","affiliation":[{"name":"Lanzhou University , , ,","place":["Lanzhou, People\u2019s Republic of China, 730000"]},{"name":"Huzhou University , , ,","place":["Huzhou, China, 313000"]},{"name":"Lanzhou University , , ,","place":["Lanzhou, China, 730000"]}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"316","published-online":{"date-parts":[[2026,9,18]]},"reference":[{"issue":"3","key":"2026093009081989300_cit1","doi-asserted-by":"publisher","first-page":"430","DOI":"10.1038\/s41559-018-0793-y","article-title":"The global burden of pathogens and pests on major food\ncrops","volume":"3","author":"Savary","year":"2019","journal-title":"Nat. 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Plant Pathol."},{"issue":"11","key":"2026093009081989300_cit3","doi-asserted-by":"publisher","first-page":"929","DOI":"10.1016\/j.tim.2018.06.002","article-title":"How Ralstonia\nsolanacearum Exploits\nand Thrives in the Flowing Plant Xylem Environment","volume":"26","author":"Lowe-Power","year":"2018","journal-title":"Trends Microbiol."},{"issue":"1","key":"2026093009081989300_cit4","doi-asserted-by":"publisher","first-page":"1","DOI":"10.1264\/jsme2.ME14144","article-title":"Recent trends\nin control methods for bacterial wilt diseases caused by Ralstonia\nsolanacearum","volume":"30","author":"Yuliar","year":"2015","journal-title":"Microbes Environ."},{"issue":"3","key":"2026093009081989300_cit5","doi-asserted-by":"publisher","first-page":"770","DOI":"10.1021\/acs.jnatprod.9b01285","article-title":"Natural Products\nas Sources of New Drugs over the Nearly\nFour Decades from 01\/1981 to 09\/2019","volume":"83","author":"Newman","year":"2020","journal-title":"J. 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Food Chem."},{"issue":"10","key":"2026093009081989300_cit18","doi-asserted-by":"publisher","first-page":"1377","DOI":"10.1111\/mpp.12977","article-title":"The large, diverse, and robust arsenal of Ralstonia\nsolanacearum type III effectors and their in planta functions","volume":"21","author":"Landry","year":"2020","journal-title":"Mol. Plant Pathol."},{"key":"2026093009081989300_cit19","doi-asserted-by":"publisher","first-page":"2437","DOI":"10.3791\/2437","article-title":"Microtiter\ndish biofilm formation assay","volume":"47","author":"O\u2019Toole","year":"2011","journal-title":"J. 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Plant-Microbe Interact."},{"issue":"7","key":"2026093009081989300_cit22","doi-asserted-by":"publisher","first-page":"6310","DOI":"10.1021\/acs.jafc.5c05956","article-title":"Adaptive\nLaboratory Evolution of Baker\u2019s Yeast for Improved Trehalose\nAccumulation and Freeze-Thaw Tolerance","volume":"74","author":"Yook","year":"2026","journal-title":"J. Agric.\nFood Chem."},{"key":"2026093009081989300_cit23","doi-asserted-by":"publisher","first-page":"57","DOI":"10.1007\/978-1-60761-411-1_4","article-title":"Identification of ROS using oxidized\nDCFDA and flow-cytometry","volume":"594","author":"Eruslanov","year":"2010","journal-title":"Methods Mol. 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Agric. Food Chem."},{"issue":"1","key":"2026093009081989300_cit27","doi-asserted-by":"publisher","DOI":"10.1038\/s41598-024-76765-4","article-title":"Evaluating the impact\nof different biochar types on wheat germination","volume":"14","author":"Kataya","year":"2024","journal-title":"Sci. Rep."},{"key":"2026093009081989300_cit28","doi-asserted-by":"publisher","first-page":"967","DOI":"10.3389\/fpls.2017.00967","article-title":"Regulation Involved\nin Colonization\nof Intercellular Spaces of Host Plants in Ralstonia solanacearum","volume":"8","author":"Hikichi","year":"2017","journal-title":"Front. Plant Sci."},{"issue":"1","key":"2026093009081989300_cit29","doi-asserted-by":"publisher","first-page":"139","DOI":"10.1093\/bioinformatics\/btp616","article-title":"edgeR: a Bioconductor package for\ndifferential expression analysis of digital gene expression data","volume":"26","author":"Robinson","year":"2010","journal-title":"Bioinformatics."},{"issue":"1","key":"2026093009081989300_cit30","doi-asserted-by":"publisher","first-page":"41","DOI":"10.1038\/s41592-019-0638-x","article-title":"DIA-NN: neural\nnetworks and interference correction enable deep proteome coverage\nin high throughput","volume":"17","author":"Demichev","year":"2020","journal-title":"Nat. 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Phytopathol."},{"issue":"11","key":"2026093009081989300_cit36","doi-asserted-by":"publisher","first-page":"4103","DOI":"10.1111\/1462-2920.13446","article-title":"Extracellular\nDNases of Ralstonia solanacearum modulate biofilms and facilitate\nbacterial wilt virulence","volume":"18","author":"Minh Tran","year":"2016","journal-title":"Environ. Microbiol."}],"container-title":["Journal of Agricultural\nand Food Chemistry"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/pubs.acs.org\/doi\/pdf\/10.1021\/acs.jafc.6c08360","content-type":"application\/pdf","content-version":"vor","intended-application":"unspecified"},{"URL":"https:\/\/pubs.acs.org\/jafcau\/article-pdf\/74\/38\/30068\/68552642\/acs.jafc.6c08360.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"syndication"},{"URL":"https:\/\/pubs.acs.org\/jafcau\/article-pdf\/74\/38\/30068\/68552642\/acs.jafc.6c08360.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,9,30]],"date-time":"2026-09-30T13:30:49Z","timestamp":1790775049000},"score":1,"resource":{"primary":{"URL":"https:\/\/pubs.acs.org\/jafcau\/article\/74\/38\/30068\/5432520\/Xenocoumacin-1-from-Xenorhabdus-nematophila-CB6-T2"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,9,18]]},"references-count":36,"journal-issue":{"issue":"38","published-online":{"date-parts":[[2026,9,18]]},"published-print":{"date-parts":[[2026,9,30]]}},"URL":"https:\/\/doi.org\/10.1021\/acs.jafc.6c08360","relation":{},"ISSN":["0021-8561","1520-5118"],"issn-type":[{"value":"0021-8561","type":"print"},{"value":"1520-5118","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026,9,18]]}}}