{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,16]],"date-time":"2026-09-16T14:08:10Z","timestamp":1789567690786,"version":"build-2803163510"},"reference-count":46,"publisher":"American Chemical Society (ACS)","issue":"36","license":[{"start":{"date-parts":[[2026,9,3]],"date-time":"2026-09-03T00:00:00Z","timestamp":1788393600000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-029"},{"start":{"date-parts":[[2026,9,3]],"date-time":"2026-09-03T00:00:00Z","timestamp":1788393600000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-037"},{"start":{"date-parts":[[2026,9,3]],"date-time":"2026-09-03T00:00:00Z","timestamp":1788393600000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-045"}],"funder":[{"DOI":"10.13039\/501100003392","name":"Natural Science Foundation of Fujian Province","doi-asserted-by":"publisher","award":["2025J01602"],"award-info":[{"award-number":["2025J01602"]}],"id":[{"id":"10.13039\/501100003392","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100003392","name":"Natural Science Foundation of Fujian Province","doi-asserted-by":"publisher","award":["2026J001334"],"award-info":[{"award-number":["2026J001334"]}],"id":[{"id":"10.13039\/501100003392","id-type":"DOI","asserted-by":"publisher"}]},{"name":"State Key Laboratory of Ecological Pest Control for Fujian and Taiwan Crops","award":["SKL2023002"],"award-info":[{"award-number":["SKL2023002"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2026,9,16]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>Tannases can reduce haze and astringency in tea beverages, but their application is often limited by poor thermal stability and catalytic efficiency. A tannase from Paraburkholderia pallida (Tanpp) was engineered using active-site mutagenesis and computational hotspot prediction. The double mutant F384T\/N307A showed the best performance, with a 2.7-fold increase in catalytic efficiency and an 11-fold longer half-life at 50 \u00b0C than wild-type Tanpp. Structural modeling and molecular dynamics simulations suggested that N307A widened the substrate-entry region, whereas F384T compacted the catalytic pocket. The double mutant remained more stable at 323.15 K. In green tea clarification assays, F384T\/N307A produced the lowest turbidity and promoted conversion of galloylated catechins into non-galloylated products and gallic acid. It retained superior performance across the tested enzyme dosages and treatment times. These findings show that coordinated active-site remodeling can improve both catalytic performance and thermal durability, supporting F384T\/N307A as a promising tannase for tea clarification.<\/jats:p>","DOI":"10.1021\/acs.jafc.6c08521","type":"journal-article","created":{"date-parts":[[2026,9,4]],"date-time":"2026-09-04T00:01:47Z","timestamp":1788480107000},"page":"28679-28693","source":"Crossref","is-referenced-by-count":0,"title":["Engineering a\n                    <i>Paraburkholderia pallida<\/i>\n                    Tannase for Improved Catalytic\nEfficiency, Thermal Stability, and\nGreen Tea Clarification"],"prefix":"10.1021","volume":"74","author":[{"given":"Fuquan","family":"Gao","sequence":"first","affiliation":[{"name":"Fujian Agriculture and Forestry University , , ,","place":["Fuzhou, China, 350002"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Keman","family":"He","sequence":"additional","affiliation":[{"name":"Fujian Agriculture and Forestry University , , ,","place":["Fuzhou, China, 350002"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tingting","family":"Lin","sequence":"additional","affiliation":[{"name":"Fujian Agriculture and Forestry University , , ,","place":["Fuzhou, China, 350002"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Haorong","family":"Sun","sequence":"additional","affiliation":[{"name":"Fujian Agriculture and Forestry University , , ,","place":["Fuzhou, China, 350002"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiaoyu","family":"Hu","sequence":"additional","affiliation":[{"name":"Fujian Agriculture and Forestry University , , ,","place":["Fuzhou, China, 350002"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yaoyu","family":"Chen","sequence":"additional","affiliation":[{"name":"Fujian Agriculture and Forestry University , , ,","place":["Fuzhou, China, 350002"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Qiyu","family":"Li","sequence":"additional","affiliation":[{"name":"Fujian Agriculture and Forestry University , , ,","place":["Fuzhou, China, 350002"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xinyu","family":"You","sequence":"additional","affiliation":[{"name":"Fujian Agriculture and Forestry University , , ,","place":["Fuzhou, China, 350002"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiaxia","family":"Wang","sequence":"additional","affiliation":[{"name":"Fujian Agriculture and Forestry University , , ,","place":["Fuzhou, China, 350002"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7906-0476","authenticated-orcid":true,"given":"Si","family":"Chen","sequence":"additional","affiliation":[{"name":"Fujian Agriculture and Forestry University , , ,","place":["Fuzhou, China, 350002"]},{"name":"Fujian Agriculture and Forestry University , , ,","place":["Fuzhou, China, 350002"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8650-6795","authenticated-orcid":true,"given":"Xiaomin","family":"Yu","sequence":"additional","affiliation":[{"name":"Fujian Agriculture and Forestry University , , ,","place":["Fuzhou, China, 350002"]},{"name":"Fujian Agriculture and Forestry University , , ,","place":["Fuzhou, China, 350002"]}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"316","published-online":{"date-parts":[[2026,9,3]]},"reference":[{"key":"2026091609091098000_cit1","doi-asserted-by":"publisher","DOI":"10.3390\/molecules30040800","article-title":"A comprehensive review\nof bioactive tannins in foods and beverages: functional properties,\nhealth benefits, and sensory qualities","volume":"30","author":"Cosme","year":"2025","journal-title":"Molecules"},{"key":"2026091609091098000_cit2","doi-asserted-by":"publisher","first-page":"2164","DOI":"10.1111\/1541-4337.12572","article-title":"Effects of edible plant\npolyphenols on gluten protein functionality\nand potential applications of polyphenol\u2013gluten interactions","volume":"19","author":"Girard","year":"2020","journal-title":"Compr. 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