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LC-MS\/MS-guided molecular networking of Aspergillus japonicus led to the isolation of eight rare calbistrin-type polyketides (1\u20138), including three new derivatives, japonidienes J\u2013L (1\u20133). Their structures were elucidated by spectroscopic analysis and computational methods. Compounds 1, 2, and 5\u20138 were evaluated for phytotoxicity against Eleusine indica and Amaranthus retroflexus seeds. Compounds 1 and 2 inhibited E. indica radicle elongation by 89.4% and 87.8%, respectively, comparable to glyphosate, while compounds 1, 2, and 5 inhibited A. retroflexus seedling growth more strongly than glyphosate. Integrated omics, qRT-PCR, and molecular docking\/molecular dynamics simulations analyses suggested that compound 1 may affect trehalose-related metabolism through potential interaction with trehalose-6-phosphate synthase. Zebrafish embryo assays indicated relatively low developmental toxicity, supporting calbistrins as promising herbicidal leads.<\/jats:p>","DOI":"10.1021\/acs.jafc.6c08564","type":"journal-article","created":{"date-parts":[[2026,8,12]],"date-time":"2026-08-12T02:01:36Z","timestamp":1786500096000},"page":"26427-26440","source":"Crossref","is-referenced-by-count":0,"title":["Molecular Networking-Guided\nDiscovery of New Calbistrins\nfrom\n                    <i>Aspergillus japonicus<\/i>\n                    and Integrated\nOmics Analyses of Their Phytotoxic Mechanism"],"prefix":"10.1021","volume":"74","author":[{"given":"Lian-Cheng","family":"Xu","sequence":"first","affiliation":[{"name":"Suqian First Hospital , , ,","place":["Suqian, People\u2019s Republic of China, 223800"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chi","family":"Liang","sequence":"additional","affiliation":[{"name":"Suqian First Hospital , , ,","place":["Suqian, People\u2019s Republic of China, 223800"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yu","family":"Chen","sequence":"additional","affiliation":[{"name":"Suqian First Hospital , , ,","place":["Suqian, People\u2019s Republic of China, 223800"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yan-Xia","family":"Zhang","sequence":"additional","affiliation":[{"name":"Shandong Institute for Food and Drug Control , , ,","place":["Jinan, People\u2019s Republic of China, 250101"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Guan-Yi","family":"Cao","sequence":"additional","affiliation":[{"name":"Suqian First Hospital , , ,","place":["Suqian, People\u2019s Republic of China, 223800"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8163-327X","authenticated-orcid":true,"given":"Gan","family":"Gu","sequence":"additional","affiliation":[{"name":"Jiangxi Agricultural University , , ,","place":["Nanchang, China, 330045"]}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"316","published-online":{"date-parts":[[2026,8,11]]},"reference":[{"key":"2026090104472069000_cit1","doi-asserted-by":"publisher","first-page":"107","DOI":"10.1186\/s12934-023-02118-x","article-title":"Bioactive compounds and biomedical applications of\nendophytic fungi: a recent review","volume":"22","author":"Hashem","year":"2023","journal-title":"Microb. 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