{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,21]],"date-time":"2026-10-21T13:45:24Z","timestamp":1792590324962,"version":"4.5.0"},"reference-count":51,"publisher":"American Chemical Society (ACS)","issue":"41","license":[{"start":{"date-parts":[[2026,10,12]],"date-time":"2026-10-12T00:00:00Z","timestamp":1791763200000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-029"},{"start":{"date-parts":[[2026,10,12]],"date-time":"2026-10-12T00:00:00Z","timestamp":1791763200000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-037"},{"start":{"date-parts":[[2026,10,12]],"date-time":"2026-10-12T00:00:00Z","timestamp":1791763200000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-045"}],"funder":[{"DOI":"10.13039\/na","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["U22A20542"],"award-info":[{"award-number":["U22A20542"]}],"id":[{"id":"10.13039\/na","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2026,10,21]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>Gellan gum exhibits excellent gelation properties and is a valuable hydrocolloid in the food industry. Carbohydrate-binding modules (CBMs) are important tools for polysaccharide research, particularly as probes for in situ analysis. However, no gellan gum CBM has been reported. Herein, a CBM96 domain from a putative gellan gum lyase was recombinantly expressed. The recombinant protein ClCBM96 specifically binds to gellan gum with an association constant (Ka) of 1.5 \u00d7 105 M\u20131. The crystal structure of ClCBM96 was determined at a resolution of 1.81 \u00c5, revealing a typical \u03b2-sandwich fold. Site-directed mutagenesis identified residues W28, K32, T37, and R39 as critical for gellan gum binding. A fluorescent probe constructed by fusing ClCBM96 with green fluorescent protein was validated for in situ visualization of gellan gum. In summary, this study reports the first identification and characterization of a gellan gum-specific CBM, providing a valuable tool for future research on gellan gum.<\/jats:p>","DOI":"10.1021\/acs.jafc.6c08586","type":"journal-article","created":{"date-parts":[[2026,10,13]],"date-time":"2026-10-13T04:02:02Z","timestamp":1791864122000},"page":"32195-32204","source":"Crossref","is-referenced-by-count":0,"title":["Characterization\nand Application of a Novel Gellan\nCarbohydrate Binding Module from the CBM96 Family"],"prefix":"10.1021","volume":"74","author":[{"given":"Kaimeng","family":"Liu","sequence":"first","affiliation":[{"name":"Ocean University of China , , 1299 Sansha Road , , ,","place":["Qingdao, Shandong, China, 266404"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hong","family":"Su","sequence":"additional","affiliation":[{"name":"Ocean University of China , , 1299 Sansha Road , , ,","place":["Qingdao, Shandong, China, 266404"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Menghui","family":"Sun","sequence":"additional","affiliation":[{"name":"Ocean University of China , , 1299 Sansha Road , , ,","place":["Qingdao, Shandong, China, 266404"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xuanwei","family":"Mei","sequence":"additional","affiliation":[{"name":"Shandong University of Technology , , , ,","place":["Zibo, Shandong, China, 255000"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mengyao","family":"Su","sequence":"additional","affiliation":[{"name":"Ocean University of China , , 1299 Sansha Road , , ,","place":["Qingdao, Shandong, China, 266404"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8234-7272","authenticated-orcid":true,"given":"Yaoguang","family":"Chang","sequence":"additional","affiliation":[{"name":"Ocean University of China , , 1299 Sansha Road , , ,","place":["Qingdao, Shandong, China, 266404"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"David Julian","family":"McClements","sequence":"additional","affiliation":[{"name":"University of Massachusetts Amherst , , , ,","place":["Amherst, Massachusetts, United States, 01003"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6976-2906","authenticated-orcid":true,"given":"Changhu","family":"Xue","sequence":"additional","affiliation":[{"name":"Ocean University of China , , 1299 Sansha Road , , ,","place":["Qingdao, Shandong, China, 266404"]}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"316","published-online":{"date-parts":[[2026,10,12]]},"reference":[{"key":"2026102109074646600_cit1","doi-asserted-by":"publisher","first-page":"240","DOI":"10.1080\/21655979.2019.1628882","article-title":"Screening\nand enzymatic activity of high-efficiency gellan lyase producing bacteria\nPseudoalteromonas hodoensis PE1","volume":"10","author":"Li","year":"2019","journal-title":"Bioengineered"},{"key":"2026102109074646600_cit2","doi-asserted-by":"publisher","first-page":"889","DOI":"10.1007\/s00253-008-1496-0","article-title":"Occurrence,\nproduction, and applications of gellan:\ncurrent state and perspectives","volume":"79","author":"Fialho","year":"2008","journal-title":"Appl. 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J."},{"key":"2026102109074646600_cit50","doi-asserted-by":"publisher","first-page":"37748","DOI":"10.1074\/jbc.M606126200","article-title":"The Interaction\nof a Carbohydrate-binding Module from\na Clostridium perfringens N-Acetyl-\u03b2-hexosaminidase with Its\nCarbohydrate Receptor","volume":"281","author":"Ficko-Blean","year":"2006","journal-title":"J. Biol. Chem."},{"key":"2026102109074646600_cit51","doi-asserted-by":"publisher","first-page":"10395","DOI":"10.1021\/bi9013424","article-title":"Analysis\nof the structural and functional diversity of plant cell wall specific\nfamily 6 carbohydrate binding modules","volume":"48","author":"Abbott","year":"2009","journal-title":"Biochemistry"}],"container-title":["Journal of Agricultural\nand Food Chemistry"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/pubs.acs.org\/doi\/pdf\/10.1021\/acs.jafc.6c08586","content-type":"application\/pdf","content-version":"vor","intended-application":"unspecified"},{"URL":"https:\/\/pubs.acs.org\/jafcau\/article-pdf\/74\/41\/32195\/68827893\/acs.jafc.6c08586.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"syndication"},{"URL":"https:\/\/pubs.acs.org\/jafcau\/article-pdf\/74\/41\/32195\/68827893\/acs.jafc.6c08586.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,10,21]],"date-time":"2026-10-21T13:15:36Z","timestamp":1792588536000},"score":1,"resource":{"primary":{"URL":"https:\/\/pubs.acs.org\/jafcau\/article\/74\/41\/32195\/5449436\/Characterization-and-Application-of-a-Novel-Gellan"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,10,12]]},"references-count":51,"journal-issue":{"issue":"41","published-online":{"date-parts":[[2026,10,12]]},"published-print":{"date-parts":[[2026,10,21]]}},"URL":"https:\/\/doi.org\/10.1021\/acs.jafc.6c08586","relation":{},"ISSN":["0021-8561","1520-5118"],"issn-type":[{"value":"0021-8561","type":"print"},{"value":"1520-5118","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026,10,12]]}}}