{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,16]],"date-time":"2026-09-16T14:04:15Z","timestamp":1789567455871,"version":"build-2803163510"},"reference-count":44,"publisher":"American Chemical Society (ACS)","issue":"36","license":[{"start":{"date-parts":[[2026,9,7]],"date-time":"2026-09-07T00:00:00Z","timestamp":1788739200000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-029"},{"start":{"date-parts":[[2026,9,7]],"date-time":"2026-09-07T00:00:00Z","timestamp":1788739200000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-037"},{"start":{"date-parts":[[2026,9,7]],"date-time":"2026-09-07T00:00:00Z","timestamp":1788739200000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-045"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["32172641"],"award-info":[{"award-number":["32172641"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["32472414"],"award-info":[{"award-number":["32472414"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["32472804"],"award-info":[{"award-number":["32472804"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2026,9,16]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>Citrus fruits are susceptible to infection by Penicillium digitatum, causing significant economic loss. Exploring eco-friendly control strategies is therefore critical. Here, we identified and heterologously expressed a novel secreted protein, PdCP1, from P. digitatum and characterized it as a proteinaceous elicitor. Exogenous application of PdCP1 to citrus fruit significantly inhibited lesion development without direct antifungal activity, effectively preserving postharvest quality. PdCP1 triggered a series of defense responses, including reactive oxygen species bursts, callose deposition, and upregulation of pattern-triggered immunity genes. Furthermore, it enhanced the activity and transcript levels of key defense enzymes, upregulated the expression of critical genes in salicylic acid and jasmonic acid signaling pathways, and elevated endogenous hormone levels. PdCP1-induced resistance was also effective in other fruits tested against fungal pathogens. These findings demonstrated that PdCP1 acts as a potent protein elicitor, providing a novel and sustainable strategy for controlling postharvest decay via an induced resistance.<\/jats:p>","DOI":"10.1021\/acs.jafc.6c08759","type":"journal-article","created":{"date-parts":[[2026,9,7]],"date-time":"2026-09-07T08:02:25Z","timestamp":1788768145000},"page":"28351-28361","source":"Crossref","is-referenced-by-count":0,"title":["PdCP1 from\n                    <i>Penicillium digitatum<\/i>\n                    Acts\nas a Protein Elicitor to Control Postharvest Decay and Preserve\nQuality in Citrus Fruit"],"prefix":"10.1021","volume":"74","author":[{"given":"Xue","family":"Bai","sequence":"first","affiliation":[{"name":"Jiangsu University , , 301 Xuefu Road , ,","place":["Zhenjiang, China, 212013"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xi","family":"Zhang","sequence":"additional","affiliation":[{"name":"Jiangsu University , , 301 Xuefu Road , ,","place":["Zhenjiang, China, 212013"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Solairaj","family":"Dhanasekaran","sequence":"additional","affiliation":[{"name":"PSGR Krishnammal College for Women , , ,","place":["Coimbatore, India, 641004"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xu","family":"Fan","sequence":"additional","affiliation":[{"name":"Jiangsu University , , 301 Xuefu Road , ,","place":["Zhenjiang, China, 212013"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chenggong","family":"Liu","sequence":"additional","affiliation":[{"name":"Jiangsu University , , 301 Xuefu Road , ,","place":["Zhenjiang, China, 212013"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Qiya","family":"Yang","sequence":"additional","affiliation":[{"name":"Jiangsu University , , 301 Xuefu Road , ,","place":["Zhenjiang, China, 212013"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6906-4713","authenticated-orcid":true,"given":"Hongyin","family":"Zhang","sequence":"additional","affiliation":[{"name":"Jiangsu University , , 301 Xuefu Road , ,","place":["Zhenjiang, China, 212013"]}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"316","published-online":{"date-parts":[[2026,9,7]]},"reference":[{"key":"2026091609090055500_cit1","doi-asserted-by":"publisher","first-page":"112673","DOI":"10.1016\/j.postharvbio.2023.112673","article-title":"Xyloglucan-Specific\nEndo-\u03b2-1,4-Glucanase (PdXEG1)\nGene Is Important for the Growth, Development and Virulence of Penicillium digitatum","volume":"208","author":"Wang","year":"2024","journal-title":"Postharvest\nBiol. Technol."},{"key":"2026091609090055500_cit2","doi-asserted-by":"publisher","first-page":"833328","DOI":"10.3389\/fpls.2021.833328","article-title":"Alternative\nManagement Approaches of Citrus Diseases Caused by Penicillium digitatum (Green Mold) and Penicillium italicum (Blue Mold)","volume":"12","author":"Bhatta","year":"2022","journal-title":"Front. Plant Sci."},{"key":"2026091609090055500_cit3","doi-asserted-by":"publisher","first-page":"105835","DOI":"10.1016\/j.pestbp.2024.105835","article-title":"Octanal Enhances\nDisease Resistance in Postharvest Citrus Fruit by the Biosynthesis\nand Metabolism of Aromatic Amino Acids","volume":"200","author":"Duan","year":"2024","journal-title":"Pestic.\nBiochem. Physiol."},{"key":"2026091609090055500_cit4","doi-asserted-by":"publisher","first-page":"1541448","DOI":"10.3389\/fmicb.2025.1541448","article-title":"Protein Elicitor PeVn1 Induces Resistance to Botrytis cinerea in Strawberry and Differential Transcriptomic Analysis","volume":"16","author":"Zhang","year":"2025","journal-title":"Front. Microbiol."},{"issue":"7","key":"2026091609090055500_cit5","doi-asserted-by":"publisher","first-page":"467","DOI":"10.3390\/jof11070467","article-title":"Elicitor from Trichothecium roseum Activates the\nDisease Resistance of Salicylic Acid, Jasmonic Acid, and Ca2\u00b1Dependent Pathways in Potato Tubers","volume":"11","author":"Wang","year":"2025","journal-title":"J. Fungi"},{"key":"2026091609090055500_cit6","doi-asserted-by":"publisher","first-page":"149622","DOI":"10.1016\/j.ijbiomac.2025.149622","article-title":"Levan Induced the Resistance\nof Tomato against Botrytis cinerea and Pepper against Phytophthora Capsici","volume":"337","author":"Yang","year":"2026","journal-title":"Int. J. Biol.\nMacromol."},{"key":"2026091609090055500_cit7","doi-asserted-by":"publisher","first-page":"1064589","DOI":"10.3389\/fpls.2022.1064589","article-title":"A Novel Elicitor\nProtein Phosphopentomutase from Bacillus velezensis LJ02 Enhances Tomato Resistance to Botrytis cinerea","volume":"13","author":"Li","year":"2022","journal-title":"Front. Plant Sci."},{"issue":"9","key":"2026091609090055500_cit8","doi-asserted-by":"publisher","first-page":"5470","DOI":"10.1002\/ps.8900","article-title":"A Putative Elicitor CcHE1 from Cytospora Chrysosperma Enhances Plant Resistance to Phytopathogenic\nFungi","volume":"81","author":"Liu","year":"2025","journal-title":"Pest Manage. Sci."},{"issue":"11","key":"2026091609090055500_cit9","doi-asserted-by":"publisher","first-page":"2849","DOI":"10.3390\/ijms20112849","article-title":"The Novel Cerato-Platanin-Like Protein\nFocCP1 from Fusarium oxysporum Triggers an Immune Response in Plants","volume":"20","author":"Li","year":"2019","journal-title":"Int. J. Mol. 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Proteomics"},{"key":"2026091609090055500_cit14","doi-asserted-by":"publisher","first-page":"1880","DOI":"10.3389\/fpls.2017.01880","article-title":"The Verticillium dahliae SnodProt1-Like Protein\nVdCP1 Contributes to Virulence and Triggers the Plant Immune System","volume":"8","author":"Zhang","year":"2017","journal-title":"Front. Plant Sci."},{"issue":"4","key":"2026091609090055500_cit15","doi-asserted-by":"publisher","first-page":"299","DOI":"10.1094\/MPMI-12-15-0266-R","article-title":"Magnaporthe oryzae -Secreted Protein\nMSP1 Induces Cell Death and Elicits Defense Responses\nin Rice","volume":"29","author":"Wang","year":"2016","journal-title":"Mol. Plant-Microbe Interact."},{"issue":"8","key":"2026091609090055500_cit16","doi-asserted-by":"publisher","first-page":"838","DOI":"10.1094\/MPMI-19-0838","article-title":"Sm1, a\nProteinaceous Elicitor Secreted by the Biocontrol Fungus Trichoderma Virens Induces Plant Defense Responses and Systemic Resistance","volume":"19","author":"Djonovic\u0301","year":"2006","journal-title":"Mol. 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Int."},{"issue":"8","key":"2026091609090055500_cit23","doi-asserted-by":"publisher","first-page":"2619","DOI":"10.1021\/acs.jafc.0c06283","article-title":"Pichia galeiformis Induces Resistance in Postharvest Citrus by Activating\nthe Phenylpropanoid Biosynthesis Pathway","volume":"69","author":"Chen","year":"2021","journal-title":"J.\nAgric. Food Chem."},{"key":"2026091609090055500_cit24","doi-asserted-by":"publisher","first-page":"112975","DOI":"10.1016\/j.scienta.2024.112975","article-title":"Bacillus siamensis N-1 Improves Fruit Quality and\nDisease Resistance by Regulating ROS Homeostasis and Defense Enzyme\nActivities in Pitaya","volume":"329","author":"Xu","year":"2024","journal-title":"Sci. Hortic."},{"key":"2026091609090055500_cit25","doi-asserted-by":"publisher","first-page":"135","DOI":"10.1021\/acs.jafc.4c07212","article-title":"Enhancing\nFruit Resistance against Fungal Pathogens Using a Pathogen-Associated\nMolecular Pattern PdEIX","volume":"73","author":"He","year":"2025","journal-title":"J. Agric. Food Chem."},{"key":"2026091609090055500_cit26","doi-asserted-by":"publisher","first-page":"117012","DOI":"10.1016\/j.foodres.2025.117012","article-title":"In Vitro Bioaccessibility of Carotenoids in Satsuma\nMandarin Juice: Effects of High Hydrostatic Pressure and It-Mediated\nMatrix Changes","volume":"219","author":"Huang","year":"2025","journal-title":"Food Res. Int."},{"issue":"6","key":"2026091609090055500_cit27","doi-asserted-by":"publisher","DOI":"10.1093\/hr\/uhaf074","article-title":"CYP98A Monooxygenases:\nA Key Enzyme Family in Plant Phenolic Compound Biosynthesis","volume":"12","author":"Zhou","year":"2025","journal-title":"Hortic. Res."},{"key":"2026091609090055500_cit28","doi-asserted-by":"publisher","first-page":"104719","DOI":"10.1016\/j.pestbp.2020.104719","article-title":"A Novel Glycoprotein from Streptomyces sp. Triggers\nEarly Responses of Plant Defense","volume":"171","author":"Han","year":"2021","journal-title":"Pestic. Biochem.\nPhysiol."},{"key":"2026091609090055500_cit29","doi-asserted-by":"publisher","first-page":"769","DOI":"10.3389\/fpls.2014.00769","article-title":"Cerato-Platanin\nFamily Proteins: One Function for Multiple Biological Roles?","volume":"5","author":"Baccelli","year":"2014","journal-title":"Front. Plant Sci."},{"key":"2026091609090055500_cit30","doi-asserted-by":"publisher","first-page":"139669","DOI":"10.1016\/j.foodchem.2024.139669","article-title":"Sanxiapeptin Is an Ideal Preservative\nwith a Dual Effect of Controlling Green Mold and Inducing Systemic\nDefense in Postharvest Citrus","volume":"453","author":"Li","year":"2024","journal-title":"Food Chem."},{"issue":"5","key":"2026091609090055500_cit31","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0178337","article-title":"A Single Amino\nAcid Mutation Affects Elicitor and Expansins-like\nActivities of Cerato-Platanin, a Non-Catalytic Fungal Protein","volume":"12","author":"Luti","year":"2017","journal-title":"PLoS One"},{"key":"2026091609090055500_cit32","doi-asserted-by":"publisher","first-page":"114150","DOI":"10.1016\/j.postharvbio.2025.114150","article-title":"Thioredoxin\nPgTRX Acts as a Protein\nElicitor to Suppress Green Mold in Citrus Fruit via Modulation of\nGlutathione Metabolism","volume":"234","author":"Zhu","year":"2026","journal-title":"Postharvest Biol. Technol."},{"key":"2026091609090055500_cit33","doi-asserted-by":"publisher","first-page":"102302","DOI":"10.1016\/j.fochx.2025.102302","article-title":"Natural Wax-Based\nEdible Coatings for Preserving Postharvest Quality\nof Mandarin Orange","volume":"26","author":"Devi","year":"2025","journal-title":"Food Chem. X"},{"key":"2026091609090055500_cit34","doi-asserted-by":"publisher","first-page":"2673","DOI":"10.1111\/pbi.70517","article-title":"3-Methyl-1-Butanol\nEnhances Postharvest\nResistance of Red Grapes to Botrytis cinerea by Activating\nPhenylpropanoid Metabolism and Antioxidant Defences","volume":"24","author":"Zhai","year":"2026","journal-title":"Plant Biotechnol. J."},{"key":"2026091609090055500_cit35","doi-asserted-by":"publisher","first-page":"102115","DOI":"10.1016\/j.fochx.2024.102115","article-title":"Blue Light\nIrradiation Combined with Low-Temperature\nStorage Further Enhances Postharvest Quality of Strawberries through\nImproving Antioxidant Defense and Cell Wall Metabolic Activities","volume":"25","author":"Lu","year":"2025","journal-title":"Food Chem. X"},{"key":"2026091609090055500_cit36","doi-asserted-by":"publisher","first-page":"208","DOI":"10.1016\/j.foodchem.2018.01.100","article-title":"Effect of Cryptococcus laurentii on Inducing Disease\nResistance in Cherry Tomato Fruit with Focus on the Expression of\nDefense-Related Genes","volume":"254","author":"Lai","year":"2018","journal-title":"Food Chem."},{"key":"2026091609090055500_cit37","doi-asserted-by":"publisher","first-page":"105","DOI":"10.1016\/j.postharvbio.2019.04.021","article-title":"(1\u21923)-\u03b2-D-Glucan\nfrom Yeast Cell Wall: Characteristic and Potential Application in\nControlling Postharvest Disease of Pear","volume":"154","author":"Sun","year":"2019","journal-title":"Postharvest\nBiol. Technol."},{"issue":"1","key":"2026091609090055500_cit38","doi-asserted-by":"publisher","first-page":"44","DOI":"10.1186\/s40529-017-0198-2","article-title":"Synergistic\nEffects of Plant Defense Elicitors and Trichoderma harzianum on Enhanced Induction of Antioxidant Defense System in Tomato against\nFusarium Wilt Disease","volume":"58","author":"Zehra","year":"2017","journal-title":"Bot Stud."},{"key":"2026091609090055500_cit39","doi-asserted-by":"publisher","first-page":"773","DOI":"10.1111\/jipb.13820","article-title":"Salicylic Acid: The Roles in Plant\nImmunity and Crosstalk with Other Hormones","volume":"67","author":"Tian","year":"2025","journal-title":"J. Integr. Plant Biol."},{"issue":"1","key":"2026091609090055500_cit40","doi-asserted-by":"publisher","first-page":"249","DOI":"10.1104\/pp.105.072348","article-title":"The Outcomes\nof Concentration-Specific Interactions between Salicylate and Jasmonate\nSignaling Include Synergy, Antagonism, and Oxidative Stress Leading\nto Cell Death","volume":"140","author":"Mur","year":"2006","journal-title":"Plant Physiol."},{"issue":"6","key":"2026091609090055500_cit41","doi-asserted-by":"publisher","DOI":"10.4161\/psb.24260","article-title":"Jasmonic Acid\nand Salicylic Acid Activate a Common Defense System in Rice","volume":"8","author":"Tamaoki","year":"2013","journal-title":"Plant. Signal. Behav."},{"issue":"1","key":"2026091609090055500_cit42","doi-asserted-by":"publisher","first-page":"7974","DOI":"10.1038\/s41598-018-26328-1","article-title":"Functional Analysis of Eliciting\nPlant Response Protein Epl1-Tas\nfrom Trichoderma asperellum ACCC30536","volume":"8","author":"Yu","year":"2018","journal-title":"Sci. Rep."},{"issue":"13","key":"2026091609090055500_cit43","doi-asserted-by":"publisher","first-page":"2443","DOI":"10.3390\/plants12132443","article-title":"Expression\nof EPL1 from Trichoderma atroviride in Arabidopsis Confers Resistance to Bacterial and Fungal Pathogens","volume":"12","author":"Rojas Moreno","year":"2023","journal-title":"Plants"},{"issue":"7","key":"2026091609090055500_cit44","doi-asserted-by":"publisher","DOI":"10.1093\/hr\/uhaf082","article-title":"Salicylic Acid and\nJasmonic Acid in Plant Immunity","volume":"12","author":"Zhang","year":"2025","journal-title":"Hortic. Res."}],"container-title":["Journal of Agricultural\nand Food Chemistry"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/pubs.acs.org\/doi\/pdf\/10.1021\/acs.jafc.6c08759","content-type":"application\/pdf","content-version":"vor","intended-application":"unspecified"},{"URL":"https:\/\/pubs.acs.org\/jafcau\/article-pdf\/74\/36\/28351\/68400347\/acs.jafc.6c08759.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"syndication"},{"URL":"https:\/\/pubs.acs.org\/jafcau\/article-pdf\/74\/36\/28351\/68400347\/acs.jafc.6c08759.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,9,16]],"date-time":"2026-09-16T13:11:49Z","timestamp":1789564309000},"score":1,"resource":{"primary":{"URL":"https:\/\/pubs.acs.org\/jafcau\/article\/74\/36\/28351\/5422157\/PdCP1-from-Penicillium-digitatum-Acts-as-a-Protein"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,9,7]]},"references-count":44,"journal-issue":{"issue":"36","published-online":{"date-parts":[[2026,9,7]]},"published-print":{"date-parts":[[2026,9,16]]}},"URL":"https:\/\/doi.org\/10.1021\/acs.jafc.6c08759","relation":{},"ISSN":["0021-8561","1520-5118"],"issn-type":[{"value":"0021-8561","type":"print"},{"value":"1520-5118","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026,9,7]]}}}