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The chip integrated dual-layer serpentine microchannels separated by a porous PET membrane, enabling coculture and communication between Caco-2 and RBL-2H3 cells. OVA was quantified indirectly by monitoring interleukin-6 (IL-6) released during the cellular allergic response. The screen-printed electrode was modified by diazonium grafting, followed by AuNP electrodeposition for antibody immobilization and signal amplification. The integrated platform exhibited a linear response to OVA from 0.2 to 4 pg\/mL, with a calibration equation of Ret(k\u03a9) = \u22127.450COVA(k\u03a9\u00b7mL\/pg) + 47.342 (R2 = 0.998) and a detection limit of 0.161 pg\/mL. A maximum electrochemical response was observed after 4 h of OVA stimulation, allowing dynamic monitoring of allergen-induced cellular responses. The proposed platform provides a sensitive and biomimetic strategy for response-oriented food allergen analysis.<\/jats:p>","DOI":"10.1021\/acs.jafc.6c08791","type":"journal-article","created":{"date-parts":[[2026,7,16]],"date-time":"2026-07-16T01:43:46Z","timestamp":1784166226000},"page":"23130-23139","source":"Crossref","is-referenced-by-count":2,"title":["Electrochemical\nBiosensor on a Microfluidic Coculture\nChip for Ovalbumin Detection"],"prefix":"10.1021","volume":"74","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-4639-532X","authenticated-orcid":true,"given":"Donglei","family":"Jiang","sequence":"first","affiliation":[{"name":"Nanjing University of Finance and Economics , , , ,","place":["Nanjing, Jiangsu, P.R. China, 210023"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Luwen","family":"Ye","sequence":"additional","affiliation":[{"name":"Nanjing University of Finance and Economics , , , ,","place":["Nanjing, Jiangsu, P.R. China, 210023"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zeng","family":"Feng","sequence":"additional","affiliation":[{"name":"Nanjing University of Finance and Economics , , , ,","place":["Nanjing, Jiangsu, P.R. China, 210023"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Daokun","family":"Xu","sequence":"additional","affiliation":[{"name":"Nanjing Institute for Food and Drug Control , , ,","place":["Nanjing, Jiangsu, P.R. China, 211198"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Songsong","family":"Jiang","sequence":"additional","affiliation":[{"name":"Yangzhou University , , , ,","place":["Yangzhou, Jiangsu, P.R. China, 225127"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0443-9649","authenticated-orcid":true,"given":"Lifeng","family":"Wang","sequence":"additional","affiliation":[{"name":"Nanjing University of Finance and Economics , , , ,","place":["Nanjing, Jiangsu, P.R. China, 210023"]}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"316","published-online":{"date-parts":[[2026,7,15]]},"reference":[{"key":"2026072910075829700_cit1","doi-asserted-by":"publisher","first-page":"116050","DOI":"10.1016\/j.trac.2020.116050","article-title":"Recent\nadvances and challenges in food-borne allergen detection","volume":"132","author":"Sena-Torralba","year":"2020","journal-title":"TrAC, Trends Anal. 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Food Chem."},{"issue":"12","key":"2026072910075829700_cit5","doi-asserted-by":"publisher","first-page":"2932","DOI":"10.3390\/foods10122932","article-title":"Improved sensitivity\nof allergen detection by immunoaffinity\nLC-MS\/MS using ovalbumin as a case study","volume":"10","author":"Ro\u0308der","year":"2021","journal-title":"Foods"},{"key":"2026072910075829700_cit6","doi-asserted-by":"publisher","first-page":"135562","DOI":"10.1016\/j.foodchem.2023.135562","article-title":"Post-synthesis of covalent\norganic frameworks as a hydrophilic platform for specific detection\nof egg ovalbumin under physiological pH","volume":"412","author":"Feng","year":"2023","journal-title":"Food\nChem."},{"issue":"6","key":"2026072910075829700_cit7","doi-asserted-by":"publisher","first-page":"487","DOI":"10.1016\/j.jbiosc.2023.03.008","article-title":"Development of a simple\ncultured cell\u2013anaerobic\nmicrobial co-culture system using liquid paraffin","volume":"135","author":"Umehara","year":"2023","journal-title":"J. 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Bioelectron."},{"issue":"1","key":"2026072910075829700_cit13","doi-asserted-by":"publisher","DOI":"10.1002\/eji.202249982","article-title":"Epithelial-immune\ncell interactions\nin allergic diseases","volume":"54","author":"Albrecht","year":"2024","journal-title":"Eur. J. Immunol."},{"issue":"38","key":"2026072910075829700_cit14","doi-asserted-by":"publisher","first-page":"51139","DOI":"10.1021\/acsami.4c11409","article-title":"Critical\nsuitability evaluation of Caco-2 cells for gut-on-a-chip","volume":"16","author":"Pan","year":"2024","journal-title":"ACS Appl. Mater. Interfaces"},{"issue":"1","key":"2026072910075829700_cit15","doi-asserted-by":"publisher","first-page":"187","DOI":"10.1016\/j.jaci.2012.08.002","article-title":"IL-33, but\nnot thymic stromal lymphopoietin or IL-25, is central to mite and\npeanut allergic sensitization","volume":"131","author":"Chu","year":"2013","journal-title":"J. Allergy Clin.\nImmunol."},{"issue":"5","key":"2026072910075829700_cit16","doi-asserted-by":"publisher","first-page":"521","DOI":"10.3390\/v12050521","article-title":"Innate Type 2 Responses to Respiratory\nSyncytial Virus Infection","volume":"12","author":"Norlander","year":"2020","journal-title":"Viruses"},{"issue":"1\u20136","key":"2026072910075829700_cit17","doi-asserted-by":"publisher","first-page":"244","DOI":"10.1016\/0925-4005(90)80209-I","article-title":"Miniaturized total chemical analysis\nsystems: a novel concept for chemical sensing","volume":"1","author":"Manz","year":"1990","journal-title":"Sens. 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Bioelectron."},{"key":"2026072910075829700_cit27","doi-asserted-by":"publisher","first-page":"342240","DOI":"10.1016\/j.aca.2024.342240","article-title":"A novel microfluidic\nchip integrating\nwith microcolumn array electrodes for rapid and ultrasensitive detection\nof alpha-fetoprotein","volume":"1291","author":"Ma","year":"2024","journal-title":"Anal. Chim. 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Acta"}],"container-title":["Journal of Agricultural\nand Food Chemistry"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/pubs.acs.org\/doi\/pdf\/10.1021\/acs.jafc.6c08791","content-type":"application\/pdf","content-version":"vor","intended-application":"unspecified"},{"URL":"https:\/\/pubs.acs.org\/jafcau\/article-pdf\/74\/29\/23130\/65967751\/acs.jafc.6c08791.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"syndication"},{"URL":"https:\/\/pubs.acs.org\/jafcau\/article-pdf\/74\/29\/23130\/65967751\/acs.jafc.6c08791.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,7,29]],"date-time":"2026-07-29T14:25:59Z","timestamp":1785335159000},"score":1,"resource":{"primary":{"URL":"https:\/\/pubs.acs.org\/jafcau\/article\/74\/29\/23130\/5211375\/Electrochemical-Biosensor-on-a-Microfluidic"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,7,15]]},"references-count":36,"journal-issue":{"issue":"29","published-online":{"date-parts":[[2026,7,15]]},"published-print":{"date-parts":[[2026,7,29]]}},"URL":"https:\/\/doi.org\/10.1021\/acs.jafc.6c08791","relation":{},"ISSN":["0021-8561","1520-5118"],"issn-type":[{"value":"0021-8561","type":"print"},{"value":"1520-5118","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026,7,15]]}}}