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Here, we developed a near-infrared supramolecular probe, M@\u03b3-CD, for rapid, noninvasive, and sensitive detection of \u03b1-amylase activity. The probe was formed through host\u2013guest complexation between \u03b3-cyclodextrin (\u03b3-CD) and a donor\u2212\u03c0\u2013acceptor fluorophore. M@\u03b3-CD reliably detected \u03b1-amylase under physiological conditions and at low concentrations in vitro. Using M@\u03b3-CD, we found that intestinal \u03b1-amylase activity in mice peaked 2 h after feeding and was elevated by high-fat and high-starch diets. Green tea administration reduced intestinal \u03b1-amylase activity, improved glucose and lipid metabolism, and alleviated obesity, suggesting that modulation of \u03b1-amylase activity may contribute to its antiobesity effects. These findings demonstrate the in vivo applicability of M@\u03b3-CD and highlight its potential for monitoring digestive enzyme dynamics and guiding metabolic interventions targeting \u03b1-amylase.<\/jats:p>","DOI":"10.1021\/acs.jafc.6c09000","type":"journal-article","created":{"date-parts":[[2026,8,11]],"date-time":"2026-08-11T14:01:33Z","timestamp":1786456893000},"page":"26838-26848","source":"Crossref","is-referenced-by-count":0,"title":["Dynamic Responses of \u03b1-Amylase to Diets\nand Their Impact on Metabolic Health: Insights from a Novel Noninvasive\nImaging"],"prefix":"10.1021","volume":"74","author":[{"given":"Beibei","family":"Wang","sequence":"first","affiliation":[{"name":"Jiangnan University , , , ,","place":["Wuxi, Jiangsu, China, 214122"]},{"name":"Jining University , , , ,","place":["Jining, Shandong, China, 273155"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Juncai","family":"Leng","sequence":"additional","affiliation":[{"name":"Jiangnan University , , , ,","place":["Wuxi, Jiangsu, China, 214122"]},{"name":"Institute of Future Food Technology, JITRI , , ,","place":["Yixing, Jiangsu, China, 214200"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9495-8508","authenticated-orcid":true,"given":"Wei","family":"Zhao","sequence":"additional","affiliation":[{"name":"Jiangnan University , , , ,","place":["Wuxi, Jiangsu, China, 214122"]},{"name":"Institute of Future Food Technology, JITRI , , ,","place":["Yixing, Jiangsu, China, 214200"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1214-1137","authenticated-orcid":true,"given":"Li","family":"Li","sequence":"additional","affiliation":[{"name":"Jiangnan University , , , ,","place":["Wuxi, Jiangsu, China, 214122"]}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"316","published-online":{"date-parts":[[2026,8,11]]},"reference":[{"key":"2026090104473778100_cit1","doi-asserted-by":"publisher","DOI":"10.1016\/j.tifs.2025.104969","article-title":"Starch Digestion:\nA Comprehensive Update on the Underlying Modulation Mechanisms and\nIts in Vitro Assessment Methodologies","volume":"159","author":"Freitas","year":"2025","journal-title":"Trends\nFood Sci. 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Pathol."},{"issue":"4","key":"2026090104473778100_cit5","doi-asserted-by":"publisher","first-page":"539","DOI":"10.1111\/cbdd.13909","article-title":"Alpha-amylase as\nMolecular Target for Treatment of\nDiabetes Mellitus: A Comprehensive Review","volume":"98","author":"Kaur","year":"2021","journal-title":"Chem.\nBiol. Drug Des."},{"issue":"1","key":"2026090104473778100_cit6","doi-asserted-by":"publisher","DOI":"10.1007\/s13659-026-00606-2","article-title":"A Review on Natural\nEnzyme Inhibitors: An Effective Approach to Control Postprandial Hyperglycemia\nin Type II Diabetes","volume":"16","author":"Shirkhan","year":"2026","journal-title":"Nat. Prod. Bioprospect."},{"issue":"1","key":"2026090104473778100_cit7","doi-asserted-by":"publisher","first-page":"1","DOI":"10.1016\/j.foodchem.2010.08.036","article-title":"In Vitro\nHuman Digestion Models for Food Applications","volume":"125","author":"Hur","year":"2011","journal-title":"Food Chem."},{"issue":"19","key":"2026090104473778100_cit8","doi-asserted-by":"publisher","first-page":"5349","DOI":"10.1080\/10408398.2021.1884841","article-title":"In Vitro Gastrointestinal Digestion and Fermentation Models\nand Their Applications in Food Carbohydrates","volume":"62","author":"Ji","year":"2022","journal-title":"Crit. Rev. Food Sci. Nutr."},{"key":"2026090104473778100_cit9","doi-asserted-by":"publisher","DOI":"10.1016\/j.bios.2020.112333","article-title":"Microdroplet Based\nDisposable Sensor\nPatch for Detection of \u03b1-Amylase in Human Blood Serum","volume":"165","author":"Bhattacharjee","year":"2020","journal-title":"Biosens. 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Bio Mater."},{"key":"2026090104473778100_cit16","doi-asserted-by":"publisher","first-page":"491","DOI":"10.1016\/j.dyepig.2017.04.004","article-title":"Highly Efficient\nFar Red\/Near-Infrared Fluorophores\nwith Aggregation-Induced Emission for Bioimaging","volume":"142","author":"Zhang","year":"2017","journal-title":"Dyes Pigm."},{"issue":"6","key":"2026090104473778100_cit17","doi-asserted-by":"publisher","first-page":"1404","DOI":"10.1021\/acs.accounts.8b00060","article-title":"Aggregation-Induced Emission (AIE) Dots: Emerging Theranostic Nanolights","volume":"51","author":"Feng","year":"2018","journal-title":"Acc. Chem. Res."},{"issue":"8","key":"2026090104473778100_cit18","doi-asserted-by":"publisher","first-page":"1554","DOI":"10.1039\/C8QM00209F","article-title":"AIE-Based Super-Resolution Imaging\nProbes for \u03b2-Amyloid Plaques in Mouse Brains","volume":"2","author":"Wang","year":"2018","journal-title":"Mater. Chem. 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Biotechnol."}],"container-title":["Journal of Agricultural and Food Chemistry"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/pubs.acs.org\/doi\/pdf\/10.1021\/acs.jafc.6c09000","content-type":"application\/pdf","content-version":"vor","intended-application":"unspecified"},{"URL":"https:\/\/pubs.acs.org\/jafcau\/article-pdf\/74\/33\/26838\/66485428\/acs.jafc.6c09000.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"syndication"},{"URL":"https:\/\/pubs.acs.org\/jafcau\/article-pdf\/74\/33\/26838\/66485428\/acs.jafc.6c09000.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,9,1]],"date-time":"2026-09-01T08:47:45Z","timestamp":1788252465000},"score":1,"resource":{"primary":{"URL":"https:\/\/pubs.acs.org\/jafcau\/article\/74\/33\/26838\/5251960\/Dynamic-Responses-of-Amylase-to-Diets-and-Their"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,8,11]]},"references-count":27,"journal-issue":{"issue":"33","published-online":{"date-parts":[[2026,8,11]]},"published-print":{"date-parts":[[2026,8,26]]}},"URL":"https:\/\/doi.org\/10.1021\/acs.jafc.6c09000","relation":{},"ISSN":["0021-8561","1520-5118"],"issn-type":[{"value":"0021-8561","type":"print"},{"value":"1520-5118","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026,8,11]]}}}