{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,14]],"date-time":"2026-10-14T12:53:08Z","timestamp":1791982388694,"version":"4.4.0"},"reference-count":43,"publisher":"American Chemical Society (ACS)","issue":"40","license":[{"start":{"date-parts":[[2026,10,2]],"date-time":"2026-10-02T00:00:00Z","timestamp":1790899200000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-029"},{"start":{"date-parts":[[2026,10,2]],"date-time":"2026-10-02T00:00:00Z","timestamp":1790899200000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-037"},{"start":{"date-parts":[[2026,10,2]],"date-time":"2026-10-02T00:00:00Z","timestamp":1790899200000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-045"}],"funder":[{"DOI":"10.13039\/501100010038","name":"Earmarked Fund for China Agriculture Research System","doi-asserted-by":"publisher","award":["CARS-03-29"],"award-info":[{"award-number":["CARS-03-29"]}],"id":[{"id":"10.13039\/501100010038","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/na","name":"National Basic Research Program of China","doi-asserted-by":"publisher","award":["2022YFD1400100"],"award-info":[{"award-number":["2022YFD1400100"]}],"id":[{"id":"10.13039\/na","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2026,10,14]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>Fusarium head blight (FHB) caused by Fusarium graminearum species complex (FGSC) is a devastating disease worldwide. The new succinate dehydrogenase inhibitor pydiflumetofen is highly effective against FHB, but its growing use raises resistance concerns. In this study, we identified four resistant Fusarium asiaticum isolates from 4088 field isolates collected across eight provinces in China in 2025. Gene replacement tests confirmed that the single-point mutation A83V of FaSdhC2 confers resistance. Notably, the resistant isolates did not exhibit significant fitness costs related to pydiflumetofen-resistance in terms of mycelial growth, sporulation, pathogenicity, and stress tolerance. To facilitate field monitoring, we developed a loop-mediated isothermal amplification (LAMP) assay targeting the FaSdhC2A83V mutation. This method is easy to operate, provides visual results within 55 min of amplification and is 100-fold more sensitive than conventional PCR. In conclusion, the LAMP assay established in this study provides a practical tool for rapid on-site monitoring of fungicide resistance and can effectively guide the rational use of fungicides in the field.<\/jats:p>","DOI":"10.1021\/acs.jafc.6c09029","type":"journal-article","created":{"date-parts":[[2026,10,3]],"date-time":"2026-10-03T04:01:37Z","timestamp":1791000097000},"page":"31491-31502","source":"Crossref","is-referenced-by-count":0,"title":["<i>Fusarium asiaticum<\/i>\n                    FaSdhC2 Point\nMutation A83V Confers Resistance to Pydiflumetofen and Development\nof a Rapid LAMP Detection Method"],"prefix":"10.1021","volume":"74","author":[{"given":"Yudi","family":"Ma","sequence":"first","affiliation":[{"name":"Zhejiang University , , ,","place":["Hangzhou, China, 310058"]},{"name":"Zhejiang University , , ,","place":["Hangzhou, China, 310058"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Qiaowan","family":"Chen","sequence":"additional","affiliation":[{"name":"Zhejiang University , , ,","place":["Hangzhou, China, 310058"]},{"name":"Zhejiang University , , ,","place":["Hangzhou, China, 310058"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Guiping","family":"Zhang","sequence":"additional","affiliation":[{"name":"Zhejiang University , , ,","place":["Hangzhou, China, 310058"]},{"name":"Zhejiang University , , ,","place":["Hangzhou, China, 310058"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xin","family":"Chen","sequence":"additional","affiliation":[{"name":"Zhejiang University , , ,","place":["Hangzhou, China, 310058"]},{"name":"Zhejiang University , , ,","place":["Hangzhou, China, 310058"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chaowen","family":"Zhang","sequence":"additional","affiliation":[{"name":"Zhejiang University , , ,","place":["Hangzhou, China, 310058"]},{"name":"Zhejiang University , , ,","place":["Hangzhou, China, 310058"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7671-637X","authenticated-orcid":true,"given":"Shuodan","family":"Hu","sequence":"additional","affiliation":[{"name":"Zhejiang University , , ,","place":["Hangzhou, China, 310058"]},{"name":"Zhejiang University , , ,","place":["Hangzhou, China, 310058"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5426-0997","authenticated-orcid":true,"given":"Zhonghua","family":"Ma","sequence":"additional","affiliation":[{"name":"Zhejiang University , , ,","place":["Hangzhou, China, 310058"]},{"name":"Zhejiang University , , ,","place":["Hangzhou, China, 310058"]}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"316","published-online":{"date-parts":[[2026,10,2]]},"reference":[{"issue":"8","key":"2026101408090359800_cit1","doi-asserted-by":"publisher","first-page":"1172","DOI":"10.1094\/PDIS-11-11-0974-RE","article-title":"Geographic\ndistribution of trichothecene chemotypes\nof the Fusarium graminearum species complex in major\nwinter wheat production areas of China","volume":"96","author":"Shen","year":"2012","journal-title":"Plant\nDis."},{"issue":"21","key":"2026101408090359800_cit2","doi-asserted-by":"publisher","DOI":"10.3390\/ijms242116005","article-title":"Identification and\nexpressional analysis of siRNAs responsive to Fusarium graminearum infection in wheat","volume":"24","author":"Fu","year":"2023","journal-title":"Int. J. Mol. 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Sci."},{"issue":"1","key":"2026101408090359800_cit7","doi-asserted-by":"publisher","first-page":"34","DOI":"10.3390\/toxins14010034","article-title":"Pydiflumetofen\nco-formulated with prothioconazole: A novel fungicide for Fusarium\nhead blight and deoxynivalenol control","volume":"14","author":"Edwards","year":"2022","journal-title":"Toxins"},{"issue":"8","key":"2026101408090359800_cit8","doi-asserted-by":"publisher","first-page":"3694","DOI":"10.1021\/acs.jafc.2c08023","article-title":"Multiple mutations\nin SDHB and SDHC2 subunits confer resistance to the succinate dehydrogenase\ninhibitor cyclobutrifluram in Fusarium fujikuroi","volume":"71","author":"Xue","year":"2023","journal-title":"J. Agric. Food. 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Plant Pathol."},{"issue":"7","key":"2026101408090359800_cit11","doi-asserted-by":"publisher","first-page":"643","DOI":"10.1016\/j.cropro.2010.02.019","article-title":"Progress in understanding molecular mechanisms and\nevolution of resistance to succinate dehydrogenase inhibiting (SDHI)\nfungicides in phytopathogenic fungi","volume":"29","author":"Avenot","year":"2010","journal-title":"Crop Prot."},{"key":"2026101408090359800_cit12","doi-asserted-by":"publisher","DOI":"10.1016\/j.ijbiomac.2024.139360","article-title":"The resistance mechanism of B_P225F\nand B_H272R mutations in succinate dehydrogenase in Botrytis cinerea","volume":"293","author":"Ma","year":"2025","journal-title":"Int. J. Biol. Macromol."},{"issue":"10","key":"2026101408090359800_cit13","doi-asserted-by":"crossref","first-page":"4018","DOI":"10.1002\/ps.7020","article-title":"Functional characterization\nof MoSdhB in conferring resistance to pydiflumetofen in blast fungus Magnaporthe oryzae","volume":"78","author":"Liu","year":"2022","journal-title":"Pest. Manage.\nSci."},{"issue":"5","key":"2026101408090359800_cit14","doi-asserted-by":"publisher","first-page":"1780","DOI":"10.1002\/ps.6795","article-title":"Fusarium graminearum FgSdhC1 point mutation A78V confers resistance\nto the succinate dehydrogenase inhibitor pydiflumetofen","volume":"78","author":"Shao","year":"2022","journal-title":"Pest. Manage. Sci."},{"key":"2026101408090359800_cit15","doi-asserted-by":"publisher","DOI":"10.1016\/j.pestbp.2025.106449","article-title":"Risk assessment\nand resistant mechanism of Fusarium graminearum to\nfluopyram","volume":"212","author":"Zhang","year":"2025","journal-title":"Pestic. Biochem. 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Plant-Microbe Interact."},{"issue":"1","key":"2026101408090359800_cit18","doi-asserted-by":"publisher","DOI":"10.1038\/srep07094","article-title":"Development and\napplication of loop-mediated isothermal amplification for detection\nof the F167Y mutation of carbendazim-resistant isolates in Fusarium graminearum","volume":"4","author":"Duan","year":"2014","journal-title":"Sci. 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Phytopathol."},{"key":"2026101408090359800_cit24","doi-asserted-by":"publisher","first-page":"237","DOI":"10.1016\/j.cropro.2017.02.011","article-title":"Sensitivity\nof Fusarium asiaticum to a novel succinate dehydrogenase\ninhibitor fungicide pydiflumetofen","volume":"96","author":"Hou","year":"2017","journal-title":"Crop Prot."},{"issue":"41","key":"2026101408090359800_cit25","doi-asserted-by":"publisher","first-page":"25848","DOI":"10.1021\/acs.jafc.5c07082","article-title":"Resistance evolution\nand fitness\nof Fusarium asiaticum from fields to succinate dehydrogenase\ninhibitor pydiflumetofen in China","volume":"73","author":"Yuan","year":"2025","journal-title":"J. Agric.\nFood. Chem."},{"issue":"3","key":"2026101408090359800_cit26","first-page":"525","article-title":"Resistance\ndetection of Fusarium graminearum species complex to pydiflumetofen\nin Anhui Province and biological characteristics of resistant populations","volume":"27","author":"Yang","year":"2025","journal-title":"Chin. J. Pestic. Sci."},{"issue":"5","key":"2026101408090359800_cit27","doi-asserted-by":"publisher","first-page":"487","DOI":"10.1094\/PHYTO-99-5-0487","article-title":"Characterization of sterol demethylation\ninhibitor-resistant Isolates of Fusarium asiaticum and F. graminearum collected from wheat in China","volume":"99","author":"Yin","year":"2009","journal-title":"Phytopathology"},{"issue":"11","key":"2026101408090359800_cit28","doi-asserted-by":"publisher","first-page":"973","DOI":"10.1016\/j.fgb.2004.08.001","article-title":"Double-joint\nPCR: a PCR-based molecular tool for gene\nmanipulations in filamentous fungi","volume":"41","author":"Yu","year":"2004","journal-title":"Fungal Genet.\nBiol."},{"key":"2026101408090359800_cit29","doi-asserted-by":"publisher","first-page":"591","DOI":"10.1007\/s00294-018-0909-6","article-title":"Involvement of the\ntwo L-lactate\ndehydrogenase in development and pathogenicity in Fusarium graminearum","volume":"65","author":"Chen","year":"2019","journal-title":"Curr. Genet."},{"issue":"1","key":"2026101408090359800_cit30","doi-asserted-by":"publisher","DOI":"10.1186\/s12866-018-1268-7","article-title":"Involvement\nof a dihydrodipicolinate synthase gene (FaDHDPS1) in fungal development,\npathogenesis and stress responses in Fusarium asiaticum","volume":"18","author":"Ren","year":"2018","journal-title":"BMC Microbiol."},{"issue":"5","key":"2026101408090359800_cit31","doi-asserted-by":"publisher","DOI":"10.1371\/journal.ppat.1012215","article-title":"FgPfn participates\nin vegetative growth, sexual reproduction, pathogenicity, and fungicides\nsensitivity via affecting both microtubules and actin in the filamentous\nfungus Fusarium graminearum","volume":"20","author":"Yuan","year":"2024","journal-title":"PLoS Pathog."},{"issue":"12","key":"2026101408090359800_cit32","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/28.12.e63","article-title":"Loop-mediated\nisothermal amplification\nof DNA","volume":"28","author":"Notomi","year":"2000","journal-title":"Nucleic Acids Res."},{"issue":"17","key":"2026101408090359800_cit33","doi-asserted-by":"publisher","first-page":"2183","DOI":"10.3390\/diagnostics15172183","article-title":"Development\nof SNP-LAMP combined\nwith lateral flow dipstick to detect the S531L rpoB gene mutation\nin rifampicin-resistant Mycobacterium tuberculosis","volume":"15","author":"Ckumdee","year":"2025","journal-title":"Diagnostics"},{"issue":"8","key":"2026101408090359800_cit34","doi-asserted-by":"publisher","first-page":"1924","DOI":"10.1039\/C7AN01897E","article-title":"Impact of\nprimer dimers and self-amplifying hairpins on reverse transcription\nloop-mediated isothermal amplification detection of viral RNA","volume":"143","author":"Meagher","year":"2018","journal-title":"Analyst"},{"issue":"3","key":"2026101408090359800_cit35","doi-asserted-by":"publisher","first-page":"167","DOI":"10.2144\/000113072","article-title":"Colorimetric\ndetection of loop-mediated isothermal amplification reaction by using\nhydroxy naphthol blue","volume":"46","author":"Goto","year":"2009","journal-title":"BioTechniques"},{"key":"2026101408090359800_cit36","doi-asserted-by":"publisher","DOI":"10.1128\/mbio.02237-23","article-title":"A novel\npoint mutation in BcOSBP1 confers resistance to the new fungicide\nSYP-14288 in Botrytis cinerea and is associated with a fitness cost","volume":"176","author":"Duan","year":"2021","journal-title":"Pestic. Biochem. Physiol."},{"key":"2026101408090359800_cit37","doi-asserted-by":"publisher","DOI":"10.1038\/s41598-017-13317-z","article-title":"Rapid on-site evaluation\nof the development of resistance to quinone outside inhibitors in Botrytis cinerea","volume":"7","author":"Hu","year":"2017","journal-title":"Sci. Rep."},{"issue":"5","key":"2026101408090359800_cit38","doi-asserted-by":"publisher","first-page":"1471","DOI":"10.1128\/JCM.03313-13","article-title":"Loop-mediated isothermal amplification\nassay for rapid diagnosis\nof malaria infections in an area of endemicity in Thailand","volume":"52","author":"Sattabongkot","year":"2014","journal-title":"J. Clin. Microbiol."},{"issue":"2","key":"2026101408090359800_cit39","doi-asserted-by":"publisher","first-page":"239","DOI":"10.1007\/s10658-010-9588-y","article-title":"Geographic substructure of Fusarium asiaticum isolates\ncollected from barley in China","volume":"127","author":"Zhang","year":"2010","journal-title":"Eur. J. Plant\nPathol."},{"issue":"4","key":"2026101408090359800_cit40","doi-asserted-by":"publisher","first-page":"580","DOI":"10.1094\/MPMI-07-19-0187-R","article-title":"Amino acid polymorphism\nin succinate dehydrogenase subunit C involved in biological fitness\nof Botrytis cinerea","volume":"33","author":"Shao","year":"2020","journal-title":"Mol. Plant-Microbe\nInteract."},{"key":"2026101408090359800_cit41","doi-asserted-by":"publisher","first-page":"24","DOI":"10.1016\/j.fgb.2014.03.006","article-title":"Fitness measurement\nreveals contrasting\ncosts in homologous recombinant mutants of Botrytis cinerea resistant to succinate dehydrogenase inhibitors","volume":"67","author":"Lale\u0300ve","year":"2014","journal-title":"Fungal Genet. Biol."},{"issue":"18","key":"2026101408090359800_cit42","doi-asserted-by":"publisher","first-page":"11340","DOI":"10.1021\/acs.jafc.5c01463","article-title":"Reference gene-assisted LAMP-LFD\nfor sensitive and specific detection of soy DNA as a marker for allergen\npresence in complex food products","volume":"73","author":"Li","year":"2025","journal-title":"J. Agric.\nFood. Chem."},{"issue":"12","key":"2026101408090359800_cit43","doi-asserted-by":"publisher","first-page":"4608","DOI":"10.1007\/s13197-020-04948-8","article-title":"Development of loop-mediated\nisothermal method and comparison with conventional PCR assay for rapid\non spot identification of tissue of cattle origin","volume":"58","author":"Kumari","year":"2021","journal-title":"J. Food Sci. Technol."}],"container-title":["Journal of Agricultural\nand Food Chemistry"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/pubs.acs.org\/doi\/pdf\/10.1021\/acs.jafc.6c09029","content-type":"application\/pdf","content-version":"vor","intended-application":"unspecified"},{"URL":"https:\/\/pubs.acs.org\/jafcau\/article-pdf\/74\/40\/31491\/68738909\/acs.jafc.6c09029.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"syndication"},{"URL":"https:\/\/pubs.acs.org\/jafcau\/article-pdf\/74\/40\/31491\/68738909\/acs.jafc.6c09029.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,10,14]],"date-time":"2026-10-14T12:25:35Z","timestamp":1791980735000},"score":1,"resource":{"primary":{"URL":"https:\/\/pubs.acs.org\/jafcau\/article\/74\/40\/31491\/5444164\/Fusarium-asiaticum-FaSdhC2-Point-Mutation-A83V"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,10,2]]},"references-count":43,"journal-issue":{"issue":"40","published-online":{"date-parts":[[2026,10,2]]},"published-print":{"date-parts":[[2026,10,14]]}},"URL":"https:\/\/doi.org\/10.1021\/acs.jafc.6c09029","relation":{},"ISSN":["0021-8561","1520-5118"],"issn-type":[{"value":"0021-8561","type":"print"},{"value":"1520-5118","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026,10,2]]}}}