{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,9]],"date-time":"2026-09-09T22:06:54Z","timestamp":1788991614935,"version":"build-2803163510"},"reference-count":39,"publisher":"American Chemical Society (ACS)","issue":"35","license":[{"start":{"date-parts":[[2026,8,25]],"date-time":"2026-08-25T00:00:00Z","timestamp":1787616000000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-029"},{"start":{"date-parts":[[2026,8,25]],"date-time":"2026-08-25T00:00:00Z","timestamp":1787616000000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-037"},{"start":{"date-parts":[[2026,8,25]],"date-time":"2026-08-25T00:00:00Z","timestamp":1787616000000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-045"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["31502123"],"award-info":[{"award-number":["31502123"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100007129","name":"Natural Science Foundation of Shandong Province","doi-asserted-by":"publisher","award":["ZR2023QC123"],"award-info":[{"award-number":["ZR2023QC123"]}],"id":[{"id":"10.13039\/501100007129","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100010870","name":"Qingdao Municipal Science and Technology Bureau","doi-asserted-by":"publisher","award":["24-1-8-xdny-21-nsh"],"award-info":[{"award-number":["24-1-8-xdny-21-nsh"]}],"id":[{"id":"10.13039\/501100010870","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100010870","name":"Qingdao Municipal Science and Technology Bureau","doi-asserted-by":"publisher","award":["24-1-8-xdny-29-nsh"],"award-info":[{"award-number":["24-1-8-xdny-29-nsh"]}],"id":[{"id":"10.13039\/501100010870","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2026,9,9]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>Widespread application of anticoagulant rodenticides (ARs) has raised public health concerns due to their toxicity and persistence. Nanobody (Nb) fusion proteins integrate target recognition and signal output for facile immunoassay development. However, whether next-generation sequencing (NGS)-identified Nbs are equally suitable for fusion-protein construction and immunoassay development as phage display-derived Nbs remains underexplored because previous studies on Nb-fusion proteins have focused exclusively on phage display-derived Nbs. Here, two high-affinity anti-AR Nbs, phage display-derived AR-Nb and NGS-identified NGS-Nb, were compared using docking, simulation, and assessments of Nb-fusion-protein binding performance. AR-Nb formed a more stable DIF complex via a channel-like pocket and localized interactions, and AR-Nb-ALP produced \u223c5-fold stronger signals than NGS-Nb-ALP. The optimized AR-Nb-ALP-based chemiluminescent enzyme immunoassay (CLEIA) detected four ARs in urine, soil, and river water with LODs of 0.26\u20131.03 ng\/mL and recoveries of 70.3\u201399.9%. This work provides a novel perspective on how different selection routes affect nanobody structure and fusion-protein performance.<\/jats:p>","DOI":"10.1021\/acs.jafc.6c09468","type":"journal-article","created":{"date-parts":[[2026,8,31]],"date-time":"2026-08-31T23:43:12Z","timestamp":1788219792000},"page":"27986-27996","source":"Crossref","is-referenced-by-count":0,"title":["Channel-Like Binding Pocket-Mediated Nanobody-Alkaline\nPhosphatase Fusion Protein for Chemiluminescent Immunoassay of Anticoagulant\nRodenticides"],"prefix":"10.1021","volume":"74","author":[{"given":"Xiaonan","family":"Wang","sequence":"first","affiliation":[{"name":"Qingdao Agricultural University , , No. 700 Changcheng Road , , ,","place":["Qingdao, People\u2019s Republic of China, 266109"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0006-6364-0664","authenticated-orcid":true,"given":"Ying","family":"Wang","sequence":"additional","affiliation":[{"name":"Qingdao Agricultural University , , No. 700 Changcheng Road , , ,","place":["Qingdao, People\u2019s Republic of China, 266109"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hong","family":"Zhang","sequence":"additional","affiliation":[{"name":"Qingdao Agricultural University , , No. 700 Changcheng Road , , ,","place":["Qingdao, People\u2019s Republic of China, 266109"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1946-430X","authenticated-orcid":true,"given":"Maksim A.","family":"Burkin","sequence":"additional","affiliation":[{"name":"I. Mechnikov Research Institute for Vaccines and Sera , , ,","place":["Moscow, Russia, 105064"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sergei Alexandrovich","family":"Eremin","sequence":"additional","affiliation":[{"name":"M.V. Lomonosov Moscow State University , , Leninsky Gory 1\/3 , , ,","place":["Moscow, Russia, 119991"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lu","family":"Xu","sequence":"additional","affiliation":[{"name":"Qingdao Agricultural University , , No. 700 Changcheng Road , , ,","place":["Qingdao, People\u2019s Republic of China, 266109"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zhicheng","family":"Sun","sequence":"additional","affiliation":[{"name":"Qingdao Agricultural University , , No. 700 Changcheng Road , , ,","place":["Qingdao, People\u2019s Republic of China, 266109"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Liuchuan","family":"Guo","sequence":"additional","affiliation":[{"name":"Qingdao Agricultural University , , No. 700 Changcheng Road , , ,","place":["Qingdao, People\u2019s Republic of China, 266109"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Qidi","family":"Zhang","sequence":"additional","affiliation":[{"name":"Qingdao Agricultural University , , No. 700 Changcheng Road , , ,","place":["Qingdao, People\u2019s Republic of China, 266109"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0167-9559","authenticated-orcid":true,"given":"Zhanhui","family":"Wang","sequence":"additional","affiliation":[{"name":"China Agricultural University , , , ,","place":["Beijing, People\u2019s Republic of China, 100193"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0103-0354","authenticated-orcid":true,"given":"Xiao","family":"Liang","sequence":"additional","affiliation":[{"name":"Qingdao Agricultural University , , No. 700 Changcheng Road , , ,","place":["Qingdao, People\u2019s Republic of China, 266109"]}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"316","published-online":{"date-parts":[[2026,8,25]]},"reference":[{"issue":"1","key":"2026090917075313400_cit1","doi-asserted-by":"publisher","DOI":"10.1128\/CMR.00044-19","article-title":"Yersinia Pestis:\nThe Natural History of Plague","volume":"34","author":"Barbieri","year":"2020","journal-title":"Clin. Microbiol.\nRev."},{"issue":"10","key":"2026090917075313400_cit2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.ppat.1001134","article-title":"Distinct Clones\nof Yersinia Pestis Caused the Black Death","volume":"6","author":"Haensch","year":"2010","journal-title":"PLoS\nPathog."},{"key":"2026090917075313400_cit3","doi-asserted-by":"publisher","first-page":"101688","DOI":"10.1016\/j.jspr.2020.101688","article-title":"Anticoagulant Rodenticides\nand Resistance\nDevelopment in Rodent Pest Species \u2013 A Comprehensive Review","volume":"88","author":"McGee","year":"2020","journal-title":"J. Stored Prod. Res."},{"key":"2026090917075313400_cit4","doi-asserted-by":"publisher","first-page":"120385","DOI":"10.1016\/j.envpol.2022.120385","article-title":"Second-Generation Anticoagulant\nRodenticides in the Blood of Obligate and Facultative European Avian\nScavengers","volume":"315","author":"Oliva-Vidal","year":"2022","journal-title":"Environ. Pollut."},{"key":"2026090917075313400_cit5","doi-asserted-by":"publisher","first-page":"122529","DOI":"10.1016\/j.jchromb.2021.122529","article-title":"Chiral\nLiquid Chromatography-Tandem\nMass Spectrometry Analysis of Superwarfarin Rodenticide Stereoisomers\n\u2013 Bromadiolone, Difenacoum and Brodifacoum \u2013 In Human\nPlasma","volume":"1165","author":"Nosal","year":"2021","journal-title":"J. Chromatogr. B"},{"key":"2026090917075313400_cit6","doi-asserted-by":"publisher","first-page":"170400","DOI":"10.1016\/j.scitotenv.2024.170400","article-title":"Exposure Assessment\nof Anticoagulant\nRodenticides in the Liver of Red Foxes (Vulpes Vulpes) in Slovenia","volume":"918","author":"Cerkvenik-Flajs","year":"2024","journal-title":"Sci. Total Environ."},{"key":"2026090917075313400_cit7","doi-asserted-by":"publisher","first-page":"146287","DOI":"10.1016\/j.scitotenv.2021.146287","article-title":"Monitoring the Hepatic Residues of\nCis- and Trans-Diastereoisomers of Second Generation Anticoagulant\nRodenticides Reveals a Different Bioaccumulation of Diastereoisomers\nin the Food Chain of the Re\u0301union Harrier (Circus Maillardi)","volume":"779","author":"Fourel","year":"2021","journal-title":"Sci. Total Environ."},{"issue":"8","key":"2026090917075313400_cit8","doi-asserted-by":"publisher","first-page":"7315","DOI":"10.1007\/s11356-018-1385-8","article-title":"First Evidence of Anticoagulant Rodenticides\nin Fish and Suspended Particulate Matter: Spatial and Temporal Distribution\nin German Freshwater Aquatic Systems","volume":"26","author":"Kotthoff","year":"2019","journal-title":"Environ.\nSci. Pollut. Res."},{"issue":"1","key":"2026090917075313400_cit9","doi-asserted-by":"publisher","first-page":"215","DOI":"10.1007\/s10311-018-0788-6","article-title":"Rating the Risks of Anticoagulant\nRodenticides in the\nAquatic Environment: A Review","volume":"17","author":"Regnery","year":"2019","journal-title":"Environ. Chem.\nLett."},{"issue":"6","key":"2026090917075313400_cit10","doi-asserted-by":"publisher","first-page":"2611","DOI":"10.1007\/s10311-024-01762-y","article-title":"Rodenticide Contamination of Cormorants\nand Mergansers\nFeeding on Wild Fish","volume":"22","author":"Regnery","year":"2024","journal-title":"Environ. Chem. Lett."},{"key":"2026090917075313400_cit11","doi-asserted-by":"publisher","first-page":"159941","DOI":"10.1016\/j.scitotenv.2022.159941","article-title":"Broad-Specificity Indirect Competitive\nEnzyme-Linked Immunosorbent Assay for Aristolochic Acids: Computer-Aided\nHapten Design and Molecular Mechanism of Antibody Recognition","volume":"859","author":"Wang","year":"2023","journal-title":"Sci. Total Environ."},{"issue":"18","key":"2026090917075313400_cit12","doi-asserted-by":"publisher","first-page":"14881","DOI":"10.1021\/acs.chemrev.1c01012","article-title":"Toward Next\nGeneration Lateral Flow Assays: Integration of Nanomaterials","volume":"122","author":"Sena-Torralba","year":"2022","journal-title":"Chem. Rev."},{"issue":"42","key":"2026090917075313400_cit13","doi-asserted-by":"publisher","first-page":"17804","DOI":"10.1021\/acs.jpcc.2c05008","article-title":"Nanomaterial\nand Interface Advances in Immunoassay Biosensors","volume":"126","author":"Mata Calidonio","year":"2022","journal-title":"J. Phys. Chem. C"},{"issue":"6428","key":"2026090917075313400_cit14","doi-asserted-by":"publisher","first-page":"446","DOI":"10.1038\/363446a0","article-title":"Naturally Occurring\nAntibodies Devoid of Light Chains","volume":"363","author":"Hamers-Casterman","year":"1993","journal-title":"Nature"},{"issue":"10","key":"2026090917075313400_cit15","doi-asserted-by":"publisher","first-page":"576","DOI":"10.3390\/biom9100576","article-title":"Development of a Simple\nPretreatment\nImmunoassay Based on an Organic Solvent-Tolerant Nanobody for the\nDetection of Carbofuran in Vegetable and Fruit Samples","volume":"9","author":"Zhang","year":"2019","journal-title":"Biomolecules"},{"issue":"17","key":"2026090917075313400_cit16","doi-asserted-by":"publisher","first-page":"8873","DOI":"10.1021\/ac502390c","article-title":"Nanobody-Based\nEnzyme Immunoassay for Aflatoxin in Agro-Products with High Tolerance\nto Cosolvent Methanol","volume":"86","author":"He","year":"2014","journal-title":"Anal. Chem."},{"issue":"52","key":"2026090917075313400_cit17","doi-asserted-by":"publisher","first-page":"20519","DOI":"10.1021\/acs.analchem.4c04799","article-title":"Development of a Nanobody\u2013Alkaline\nPhosphatase Fusion Protein for Detection of SARS-CoV-2 Spike Protein\nin a Fluorescence Enzyme Immunoassay","volume":"96","author":"Liu","year":"2024","journal-title":"Anal.\nChem."},{"key":"2026090917075313400_cit18","doi-asserted-by":"publisher","first-page":"118404","DOI":"10.1016\/j.trac.2025.118404","article-title":"Multifunctional Nanobody Fusion Proteins\nin Immunoassays: Diverse Strategies for Enhanced Analytical Performance","volume":"192","author":"He","year":"2025","journal-title":"Trends Analyt. Chem."},{"issue":"9","key":"2026090917075313400_cit19","doi-asserted-by":"publisher","first-page":"2314","DOI":"10.1002\/anie.201708459","article-title":"Nanobodies: Chemical Functionalization Strategies and\nIntracellular Applications","volume":"57","author":"Schumacher","year":"2018","journal-title":"Angew. Chem. Int.\nEd."},{"key":"2026090917075313400_cit20","doi-asserted-by":"publisher","first-page":"118786","DOI":"10.1016\/j.foodres.2026.118786","article-title":"Ratiometric\nFluorescence Nanosensor-Driven Dual-Mode Enzyme Immunoassay Based\non 9-Anthraldehyde and Mimotope Peptide-Alkaline Phosphatase Fusion\nfor Fluorescent and Visual Detection of Ochratoxin A in Pepper","volume":"231","author":"He","year":"2026","journal-title":"Food Res. Int."},{"key":"2026090917075313400_cit21","doi-asserted-by":"publisher","first-page":"105530","DOI":"10.1016\/j.jfca.2023.105530","article-title":"Expression, Purification\nof Codon-Optimized Ochratoxin A Nanobody-GST Fusion Protein and Its\nOne-Step Immunoassay for Detection of OTA in Cereal","volume":"123","author":"Cheng","year":"2023","journal-title":"J. Food Compos. Anal"},{"key":"2026090917075313400_cit22","doi-asserted-by":"publisher","first-page":"238","DOI":"10.1016\/j.envpol.2019.04.135","article-title":"Nanobody-Based\nFluorescence Resonance Energy Transfer Immunoassay for Noncompetitive\nand Simultaneous Detection of Ochratoxin a and Ochratoxin B","volume":"251","author":"Tang","year":"2019","journal-title":"Environ. Pollut."},{"key":"2026090917075313400_cit23","doi-asserted-by":"publisher","first-page":"107384","DOI":"10.1016\/j.fbio.2025.107384","article-title":"A Bioluminescent\nEnzyme Immunoassay\nBased on Nanobody-Nanoluciferase Fusion Protein for the Detection\nof Fenpropathrin","volume":"71","author":"Yang","year":"2025","journal-title":"Food Biosci."},{"issue":"10","key":"2026090917075313400_cit24","doi-asserted-by":"publisher","first-page":"539","DOI":"10.1093\/protein\/gzs060","article-title":"Antibody Isolation\nfrom Immunized Animals: Comparison\nof Phage Display and Antibody Discovery via V Gene Repertoire Mining","volume":"25","author":"Saggy","year":"2012","journal-title":"Protein Eng., Des. Sel."},{"issue":"10","key":"2026090917075313400_cit25","doi-asserted-by":"publisher","first-page":"379","DOI":"10.1093\/protein\/gzv039","article-title":"Identification\nof Cross-Reactive\nSingle-Domain Antibodies against Serum Albumin Using next-Generation\nDNA Sequencing","volume":"28","author":"Henry","year":"2015","journal-title":"Protein Eng., Des. Sel."},{"issue":"17","key":"2026090917075313400_cit26","doi-asserted-by":"publisher","first-page":"13012","DOI":"10.1021\/acs.analchem.6c00989","article-title":"Discovery\nof Nanobodies against Tetramethylenedisulfotetramine by Integrating\nNext-Generation Sequencing and Moderate Random Mutagenesis","volume":"98","author":"Gao","year":"2026","journal-title":"Anal. Chem."},{"issue":"18","key":"2026090917075313400_cit27","doi-asserted-by":"publisher","first-page":"6565","DOI":"10.3390\/molecules28186565","article-title":"Overview on\nthe Development of Alkaline-Phosphatase-Linked Optical Immunoassays","volume":"28","author":"Liu","year":"2023","journal-title":"Molecules"},{"issue":"9","key":"2026090917075313400_cit28","doi-asserted-by":"publisher","first-page":"1157","DOI":"10.1002\/jcc.20035","article-title":"Development\nand Testing of a General Amber Force Field","volume":"25","author":"Wang","year":"2004","journal-title":"J. Comput. Chem."},{"issue":"8","key":"2026090917075313400_cit29","doi-asserted-by":"publisher","first-page":"3696","DOI":"10.1021\/acs.jctc.5b00255","article-title":"ff14SB: Improving\nthe Accuracy of\nProtein Side Chain and Backbone Parameters from ff99SB","volume":"11","author":"Maier","year":"2015","journal-title":"J. Chem. Theory Comput."},{"key":"2026090917075313400_cit30","doi-asserted-by":"publisher","first-page":"19","DOI":"10.1016\/j.softx.2015.06.001","article-title":"GROMACS: High\nPerformance\nMolecular Simulations through Multi-Level Parallelism from Laptops\nto Supercomputers","volume":"1\u20132","author":"Abraham","year":"2015","journal-title":"SoftwareX"},{"key":"2026090917075313400_cit31","doi-asserted-by":"publisher","first-page":"128771","DOI":"10.1016\/j.snb.2020.128771","article-title":"Fluorescent Lateral\nFlow Immunoassay for Highly Sensitive Detection of Eight Anticoagulant\nRodenticides Based on Cadmium-Free Quantum Dot-Encapsulated Nanospheres","volume":"324","author":"Li","year":"2020","journal-title":"Sens. Actuators, B"},{"key":"2026090917075313400_cit32","doi-asserted-by":"publisher","first-page":"133257","DOI":"10.1016\/j.snb.2022.133257","article-title":"Trace Detection of Bromadiolone and\nBrodifacoum in Biological and Food Samples: A Label-Free FLuorescence\nImmunoassay Based on an in Situ Formation Strategy","volume":"380","author":"Chen","year":"2023","journal-title":"Sens. Actuators, B"},{"key":"2026090917075313400_cit33","doi-asserted-by":"publisher","first-page":"115348","DOI":"10.1016\/j.microc.2025.115348","article-title":"Computer Screening Broad-Specific Hapten for Rapid Immunochromatographic\nDetection of Multiple Residues of Second-Generation Anticoagulant\nRodenticides in Fish","volume":"218","author":"Wang","year":"2025","journal-title":"Microchem. J."},{"issue":"25","key":"2026090917075313400_cit34","doi-asserted-by":"publisher","first-page":"6755","DOI":"10.1007\/s00216-019-02051-2","article-title":"Production of a Specific\nMonoclonal Antibody and a Sensitive Immunoassay\nfor the Detection of Diphacinone in Biological Samples","volume":"411","author":"Li","year":"2019","journal-title":"Anal Bioanal Chem."},{"key":"2026090917075313400_cit35","doi-asserted-by":"publisher","first-page":"126787","DOI":"10.1016\/j.snb.2019.126787","article-title":"Novel Inner FIlter Effect-Based Fluorescence\nImmunoassay with Gold Nanoclusters for Bromadiolone Detection in Human\nSerum","volume":"297","author":"Li","year":"2019","journal-title":"Sens. Actuators, B"},{"key":"2026090917075313400_cit36","doi-asserted-by":"publisher","first-page":"137792","DOI":"10.1016\/j.foodchem.2023.137792","article-title":"A Facile Fluorescence Microplate Immunoassay Based on an in Situ Fluorogenic Reaction for the Detection of Two Highly\nToxic Anticoagulant Rodenticides in Food and Biological Matrix","volume":"437","author":"Li","year":"2024","journal-title":"Food Chem."},{"issue":"2","key":"2026090917075313400_cit37","doi-asserted-by":"publisher","first-page":"168","DOI":"10.1111\/vec.12140","article-title":"Evaluation of a\nPoint-of-care Anticoagulant Rodenticide Test for Dogs","volume":"24","author":"Istvan","year":"2014","journal-title":"J. Vet Emergen Crit Care"},{"issue":"10","key":"2026090917075313400_cit38","doi-asserted-by":"publisher","first-page":"605","DOI":"10.3390\/toxics10100605","article-title":"An Improved\nMultiple Competitive\nImmuno-SERS Sensing Platform and Its Application in Rapid Field Chemical\nToxin Screening","volume":"10","author":"Sun","year":"2022","journal-title":"Toxics"},{"issue":"14","key":"2026090917075313400_cit39","doi-asserted-by":"publisher","first-page":"3930","DOI":"10.3390\/ma14143930","article-title":"An Innovative Nanobody-Based High-Biocompatibility\nGold Interdigitated Microelectrode Electrochemical Bioimpedance Sensor\nfor the Ultrasensitive Detection of Difenacoum in Human Serum","volume":"14","author":"Guo","year":"2021","journal-title":"Materials"}],"container-title":["Journal of Agricultural and Food Chemistry"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/pubs.acs.org\/doi\/pdf\/10.1021\/acs.jafc.6c09468","content-type":"application\/pdf","content-version":"vor","intended-application":"unspecified"},{"URL":"https:\/\/pubs.acs.org\/jafcau\/article-pdf\/74\/35\/27986\/67407099\/acs.jafc.6c09468.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"syndication"},{"URL":"https:\/\/pubs.acs.org\/jafcau\/article-pdf\/74\/35\/27986\/67407099\/acs.jafc.6c09468.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,9,9]],"date-time":"2026-09-09T21:11:16Z","timestamp":1788988276000},"score":1,"resource":{"primary":{"URL":"https:\/\/pubs.acs.org\/jafcau\/article\/74\/35\/27986\/5328971\/Channel-Like-Binding-Pocket-Mediated-Nanobody"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,8,25]]},"references-count":39,"journal-issue":{"issue":"35","published-online":{"date-parts":[[2026,8,25]]},"published-print":{"date-parts":[[2026,9,9]]}},"URL":"https:\/\/doi.org\/10.1021\/acs.jafc.6c09468","relation":{},"ISSN":["0021-8561","1520-5118"],"issn-type":[{"value":"0021-8561","type":"print"},{"value":"1520-5118","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026,8,25]]}}}