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A recombinant collagenase was heterologously expressed in Escherichia coliBL21(DE3), purified, and characterized. The enzyme showed a specific activity of 231.05 U\/mg and hydrolyzed bovine bone collagen-containing matrix with a peptide yield of 58.12%. Nano-HPLC-MS\/MS identified 1449 peptides in the most active fraction. EGFR-guided virtual screening prioritized GPYGPP and GPLGPA, with docking scores of \u22128.9 and \u22128.6 kcal\/mol, respectively. At 250 \u03bcg\/mL, GPYGPP and GPLGPA significantly increased MC3T3-E1 cell proliferation to 138.10% and 139.98% of the untreated control, respectively, and promoted cell-cycle progression, alkaline phosphatase activity, matrix mineralization, and osteogenic marker expression (p &amp;lt; 0.05). Transcriptomic analysis associated GPYGPP-induced osteogenesis with extracellular matrix remodeling and VEGF, HIF-1, and cAMP-related signaling. These findings provide an integrated strategy for producing bovine bone-derived peptides and identifying food-derived osteogenic oligopeptides with potential applications in bone-health functional foods.<\/jats:p>","DOI":"10.1021\/acs.jafc.6c09495","type":"journal-article","created":{"date-parts":[[2026,9,22]],"date-time":"2026-09-22T06:02:09Z","timestamp":1790056929000},"page":"30911-30927","source":"Crossref","is-referenced-by-count":0,"title":["Recombinant Collagenase-Assisted\nProduction of Bovine\nBone-Derived Peptides and EGFR-Guided Discovery of Osteogenic Oligopeptides"],"prefix":"10.1021","volume":"74","author":[{"given":"Yanru","family":"An","sequence":"first","affiliation":[{"name":"Hefei University of Technology , , , ,","place":["Hefei, Anhui, China, 230601"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yu","family":"Wang","sequence":"additional","affiliation":[{"name":"Hefei University of Technology , , , ,","place":["Hefei, Anhui, China, 230601"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yue","family":"Sun","sequence":"additional","affiliation":[{"name":"Hefei University of Technology , , , ,","place":["Hefei, Anhui, China, 230601"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Dubilige","family":"Su","sequence":"additional","affiliation":[{"name":"Inner Mongolia Kerchin Cattle Industry Co., Ltd. , , ,","place":["Tongliao, Inner Mongolia, China, 028000"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lin","family":"Tong","sequence":"additional","affiliation":[{"name":"Inner Mongolia Kerchin Cattle Industry Co., Ltd. , , ,","place":["Tongliao, Inner Mongolia, China, 028000"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xingguang","family":"Chen","sequence":"additional","affiliation":[{"name":"Hefei University of Technology , , , ,","place":["Hefei, Anhui, China, 230601"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1173-7297","authenticated-orcid":true,"given":"Zeyu","family":"Wu","sequence":"additional","affiliation":[{"name":"Hefei University of Technology , , , ,","place":["Hefei, Anhui, China, 230601"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiaojing","family":"Wang","sequence":"additional","affiliation":[{"name":"Ningxia Academy of Agriculture and Forestry Sciences , , , ,","place":["Yinchuan, Ningxia, China, 750002"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0706-8863","authenticated-orcid":true,"given":"Baocai","family":"Xu","sequence":"additional","affiliation":[{"name":"Hefei University of Technology , , , ,","place":["Hefei, Anhui, China, 230601"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7282-658X","authenticated-orcid":true,"given":"Feiran","family":"Xu","sequence":"additional","affiliation":[{"name":"Hefei University of Technology , , , ,","place":["Hefei, Anhui, China, 230601"]}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"316","published-online":{"date-parts":[[2026,9,22]]},"reference":[{"key":"2026100711071203900_cit1","doi-asserted-by":"publisher","first-page":"111470","DOI":"10.1016\/j.lwt.2021.111470","article-title":"Extraction of low molecular weight\npeptides from bovine bone using ultrasound-assisted double enzyme\nhydrolysis: Impact on the antioxidant activities of the extracted\npeptides","volume":"146","author":"Yang","year":"2021","journal-title":"LWT"},{"key":"2026100711071203900_cit2","doi-asserted-by":"publisher","first-page":"129619","DOI":"10.1016\/j.foodchem.2021.129619","article-title":"Discrimination\nof geographical origin and species of China\u2019s cattle bones\nbased on multi-element analyses by inductively coupled plasma mass\nspectrometry","volume":"356","author":"Zhang","year":"2021","journal-title":"Food Chem."},{"key":"2026100711071203900_cit3","doi-asserted-by":"publisher","first-page":"377","DOI":"10.1002\/vms3.627","article-title":"Usage of\nmeat and bone meal in animal, poultry and fish feeds: A survey and\nrisk analysis for the occurrence of bovine spongiform encephalopathy\nin Bangladesh","volume":"8","author":"Islam","year":"2022","journal-title":"Vet. 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