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This study demonstrates that treatment with alginate oligosaccharide (AOS) could effectively retard this softening process. AOS treatment slowed down the loss of water inside the fruit, and enhancing the enzymatic activity and gene expression of antioxidants (SOD, CAT, POD, APX) to reduce the accumulation of O2\u2013, H2O2, and MDA. Concurrently, it downregulated the activities and gene expression of cell-wall-degrading enzymes (PG, Cx, \u03b2-Gal, \u03b2-Glu), which helped preserve cell-wall integrity, maintain fruit firmness, and delay softening. Furthermore, AOS treatment reduced weight loss and decay rate, stabilized soluble solids and acidity, and maintained fruit quality. Overall, the AOS treatment coordinated cell wall and ROS metabolism, and delayed softening, offering a practical strategy for extending blueberry postharvest life.<\/jats:p>","DOI":"10.1021\/acs.jafc.6c09596","type":"journal-article","created":{"date-parts":[[2026,10,6]],"date-time":"2026-10-06T16:01:46Z","timestamp":1791302506000},"page":"32663-32676","source":"Crossref","is-referenced-by-count":0,"title":["Alginate Oligosaccharide\nTreatment Delayed Postharvest\nBlueberry Softening by Modulating Water Mobility and ROS Metabolism"],"prefix":"10.1021","volume":"74","author":[{"given":"Zichong","family":"Song","sequence":"first","affiliation":[{"name":"Shenyang Agricultural University , , No. 120 Dongling Road, Shenhe District , ,","place":["Shenyang City, People\u2019s Republic of China, 110866"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hongyu","family":"Dai","sequence":"additional","affiliation":[{"name":"Shenyang Agricultural University , , No. 120 Dongling Road, Shenhe District , ,","place":["Shenyang City, People\u2019s Republic of China, 110866"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Wenxia","family":"Wang","sequence":"additional","affiliation":[{"name":"Dalian Institute of Chemical Physics, Chinese Academy of Sciences , , ,","place":["Dalian, People\u2019s Republic of China, 116023"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xin","family":"Zhou","sequence":"additional","affiliation":[{"name":"Shenyang Agricultural University , , No. 120 Dongling Road, Shenhe District , ,","place":["Shenyang City, People\u2019s Republic of China, 110866"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shujuan","family":"Ji","sequence":"additional","affiliation":[{"name":"Shenyang Agricultural University , , No. 120 Dongling Road, Shenhe District , ,","place":["Shenyang City, People\u2019s Republic of China, 110866"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6821-2040","authenticated-orcid":true,"given":"Qian","family":"Zhou","sequence":"additional","affiliation":[{"name":"Shenyang Agricultural University , , No. 120 Dongling Road, Shenhe District , ,","place":["Shenyang City, People\u2019s Republic of China, 110866"]}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"316","published-online":{"date-parts":[[2026,10,6]]},"reference":[{"key":"2026102109074033400_cit1","doi-asserted-by":"publisher","DOI":"10.1016\/j.fbio.2023.103050","article-title":"Known and\npotential health benefits and mechanisms of blueberry anthocyanins:\nA review","volume":"55","author":"Wu","year":"2023","journal-title":"Food Biosci."},{"key":"2026102109074033400_cit2","doi-asserted-by":"publisher","DOI":"10.1093\/fqsafe\/fyaf038","article-title":"Discussion on postharvest\nquality, strategies of storage and transportation techniques of blueberries:\na comprehensive review","volume":"9","author":"Cao","year":"2025","journal-title":"Food Qual. 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