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Under optimized conditions, the assay was completed within 10 min. The visual limits of detection for walnut and hazelnut proteins were 25 and 250 ng\/mL, respectively, while the instrumental limits of detection were 12 and 156 ng\/mL. The assay showed no obvious cross-reactivity with 20 nontarget food ingredients. In biscuit, sausage, and soymilk, the minimum detectable levels were 0.5 mg\/kg for walnut protein and 5 mg\/kg for hazelnut protein. These results indicate that the proposed assay is a rapid, sensitive, and practical tool for group-selective screening of walnut and\/or hazelnut protein residues in complex food matrices.<\/jats:p>","DOI":"10.1021\/acs.jafc.6c09670","type":"journal-article","created":{"date-parts":[[2026,9,4]],"date-time":"2026-09-04T16:02:22Z","timestamp":1788537742000},"page":"28795-28806","source":"Crossref","is-referenced-by-count":0,"title":["Development of\na Broad-Spectrum Fluorescent Lateral\nFlow Immunoassay for Rapid Screening of Walnut and Hazelnut Protein\nResidues in Foods"],"prefix":"10.1021","volume":"74","author":[{"given":"Qi","family":"Fu","sequence":"first","affiliation":[{"name":"Ocean University of China , , No. 1299, Sansha Road , , ,","place":["Qingdao, Shandong Province, P. R. 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