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Compound D16 (EC50 = 0.0025 \u03bcg\/mL), which was screened using an in vitro test against oomycetes, exhibited excellent inhibitory activity against P. capsici and showed little difference in efficacy compared to picarbutrazox (0.0010 \u03bcg\/mL). The control efficacy in vivo of compound D16 against P. capsici was evaluated using bell pepper fruits, leaves, and potted plants as the hosts. SEM, TEM, and fluorescence microscopy experiments indicated that compound D16, as picarbutrazox, exerted a certain degree of damage to P. capsici mycelium, leading to organelle loss and cell apoptosis. Furthermore, compound D16 at 0.05 \u03bcg\/mL potently inhibited spore germination of P. capsici by 87.2%. This research on novel piperidine amide derivatives containing a tetrazole moiety provides a valuable reference for new antioomycete agents.<\/jats:p>","DOI":"10.1021\/acs.jafc.6c09671","type":"journal-article","created":{"date-parts":[[2026,9,28]],"date-time":"2026-09-28T09:02:00Z","timestamp":1790586120000},"page":"30928-30939","source":"Crossref","is-referenced-by-count":0,"title":["Novel Piperidine\nAmide Derivatives Containing a Tetrazole\nMoiety: Safe Synthesis Methods, Molecular Design, Antioomycete Evaluation,\nand Action Mechanism"],"prefix":"10.1021","volume":"74","author":[{"ORCID":"https:\/\/orcid.org\/0009-0006-1989-9222","authenticated-orcid":true,"given":"Feng","family":"Peng","sequence":"first","affiliation":[{"name":"Nanjing Agricultural University , , ,","place":["Nanjing, China, 210095"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiangfei","family":"Fu","sequence":"additional","affiliation":[{"name":"Nanjing Agricultural University , , ,","place":["Nanjing, China, 210095"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zhizi","family":"He","sequence":"additional","affiliation":[{"name":"Nanjing Agricultural University , , ,","place":["Nanjing, China, 210095"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ying","family":"Peng","sequence":"additional","affiliation":[{"name":"Nanjing Agricultural University , , ,","place":["Nanjing, China, 210095"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tao","family":"Wang","sequence":"additional","affiliation":[{"name":"Nanjing Agricultural University , , ,","place":["Nanjing, China, 210095"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kai","family":"Yue","sequence":"additional","affiliation":[{"name":"Nanjing Agricultural University , , ,","place":["Nanjing, China, 210095"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2601-1499","authenticated-orcid":true,"given":"Min","family":"Chen","sequence":"additional","affiliation":[{"name":"Nanjing Agricultural University , , ,","place":["Nanjing, China, 210095"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4426-0077","authenticated-orcid":true,"given":"Chunlong","family":"Yang","sequence":"additional","affiliation":[{"name":"Nanjing Agricultural University , , ,","place":["Nanjing, China, 210095"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mingguo","family":"Zhou","sequence":"additional","affiliation":[{"name":"Nanjing Jixing Biotechnology Development Co., Ltd. , ,","place":["Nanjing, China, 211100"]}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"316","published-online":{"date-parts":[[2026,9,28]]},"reference":[{"key":"2026100711075750300_cit1","doi-asserted-by":"publisher","first-page":"9533","DOI":"10.1021\/acs.jafc.4c11375","article-title":"Design, synthesis,\nand anti-oomycete and antifungal activities of novel paeonol derivatives\ncontaining a pyrazole ring","volume":"73","author":"Liu","year":"2025","journal-title":"J. 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