{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,7]],"date-time":"2026-10-07T15:57:36Z","timestamp":1791388656079,"version":"4.3.3"},"reference-count":57,"publisher":"American Chemical Society (ACS)","issue":"39","license":[{"start":{"date-parts":[[2026,9,21]],"date-time":"2026-09-21T00:00:00Z","timestamp":1789948800000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-029"},{"start":{"date-parts":[[2026,9,21]],"date-time":"2026-09-21T00:00:00Z","timestamp":1789948800000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-037"},{"start":{"date-parts":[[2026,9,21]],"date-time":"2026-09-21T00:00:00Z","timestamp":1789948800000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-045"}],"funder":[{"DOI":"10.13039\/501100012166","name":"National Key Research and Development Program of China","doi-asserted-by":"publisher","award":["2025YFD1400100"],"award-info":[{"award-number":["2025YFD1400100"]}],"id":[{"id":"10.13039\/501100012166","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2026,10,7]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>Rice bakanae disease, caused by Fusarium fujikuroi, is a destructive seed-borne disease. Metconazole has excellent control potential, but its resistance risk and mechanism remain unreported. This study confirmed metconazole has strong antifungal activity, with EC50 values ranging from 0.0073 to 0.0468 \u03bcg\/mL. After fungicide taming, we obtained seven metconazole-resistant mutants with low-level, genetically stable resistance. Biological fitness showed mycelial growth and virulence did not decrease significantly, but sporulation increased significantly. Cross-resistance found the mutants had cross-resistance to tebuconazole, prochloraz, triticonazole and difenoconazole, but not to prothioconazole, prothioconazole-desthio, epoxiconazole, ipconazole, propiconazole, triadimefon, fludioxonil, or pyraclostrobin. Sequence analysis detected the G464S substitution in FfCYP51B in all mutants, which site-directed mutagenesis confirmed reduces metconazole sensitivity. Molecular docking, dynamics simulation and MM\/GBSA analysis showed the binding between metconazole and FfCYP51B-G464S was weakened, and the binding free energy was less favorable. In conclusion, the resistance risk of F. fujikuroi to metconazole is assessed as low.<\/jats:p>","DOI":"10.1021\/acs.jafc.6c09914","type":"journal-article","created":{"date-parts":[[2026,9,22]],"date-time":"2026-09-22T01:02:19Z","timestamp":1790038939000},"page":"30652-30664","source":"Crossref","is-referenced-by-count":0,"title":["Risk and Molecular\nMechanism of Metconazole Resistance\nin the Rice Bakanae Pathogen\n                    <i>Fusarium fujikuroi<\/i>"],"prefix":"10.1021","volume":"74","author":[{"ORCID":"https:\/\/orcid.org\/0009-0004-4779-3041","authenticated-orcid":true,"given":"Ziyang","family":"Zhang","sequence":"first","affiliation":[{"name":"Nanjing Agricultural University , , ,","place":["Nanjing, China, 210095"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jie","family":"Liu","sequence":"additional","affiliation":[{"name":"Nanjing Agricultural University , , ,","place":["Nanjing, China, 210095"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hui","family":"Qiu","sequence":"additional","affiliation":[{"name":"Nanjing Agricultural University , , ,","place":["Nanjing, China, 210095"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xudong","family":"Liu","sequence":"additional","affiliation":[{"name":"Nanjing Agricultural University , , ,","place":["Nanjing, China, 210095"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xianghao","family":"Meng","sequence":"additional","affiliation":[{"name":"Nanjing Agricultural University , , ,","place":["Nanjing, China, 210095"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jiakai","family":"Wang","sequence":"additional","affiliation":[{"name":"Nanjing Agricultural University , , ,","place":["Nanjing, China, 210095"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xinhao","family":"Song","sequence":"additional","affiliation":[{"name":"Nanjing Agricultural University , , ,","place":["Nanjing, China, 210095"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jie","family":"Zhang","sequence":"additional","affiliation":[{"name":"Nanjing Agricultural University , , ,","place":["Nanjing, China, 210095"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yiqiang","family":"Cai","sequence":"additional","affiliation":[{"name":"Nanjing Agricultural University , , ,","place":["Nanjing, China, 210095"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4183-8729","authenticated-orcid":true,"given":"Yabing","family":"Duan","sequence":"additional","affiliation":[{"name":"Nanjing Agricultural University , , ,","place":["Nanjing, China, 210095"]}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"316","published-online":{"date-parts":[[2026,9,21]]},"reference":[{"key":"2026100711074241800_cit1","doi-asserted-by":"publisher","first-page":"265","DOI":"10.1016\/j.tifs.2020.01.022","article-title":"Rice - not just a staple food: A\ncomprehensive review on its phytochemicals and therapeutic potential","volume":"97","author":"Sen","year":"2020","journal-title":"Trends Food Sci. 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Plant Pathol."},{"issue":"1","key":"2026100711074241800_cit47","doi-asserted-by":"publisher","first-page":"77","DOI":"10.1111\/1574-6968.12504","article-title":"Molecular\nmechanism of resistance of Fusarium fujikuroi to benzimidazole fungicides","volume":"357","author":"Chen","year":"2014","journal-title":"FEMS Microbiol.\nLett."},{"issue":"11","key":"2026100711074241800_cit48","doi-asserted-by":"publisher","DOI":"10.1093\/g3journal\/jkac249","article-title":"Analysis of Cyp51 protein\nsequences shows 4 major Cyp51 gene family groups across fungi","volume":"12","author":"Celia-Sanchez","year":"2022","journal-title":"G3:Genes, Genomes, Genet."},{"issue":"4","key":"2026100711074241800_cit49","doi-asserted-by":"publisher","DOI":"10.1371\/journal.ppat.1005464","article-title":"Fusarium: Molecular\nDiversity and Intrinsic Drug Resistance","volume":"12","author":"Al-Hatmi","year":"2016","journal-title":"PLoS\nPathog."},{"issue":"3","key":"2026100711074241800_cit50","doi-asserted-by":"publisher","first-page":"821","DOI":"10.1111\/nph.12193","article-title":"Characterization\nof the sterol 14\u03b1-demethylases of Fusarium graminearum identifies a novel genus-specific CYP51 function","volume":"198","author":"Fan","year":"2013","journal-title":"New Phytol."},{"key":"2026100711074241800_cit51","doi-asserted-by":"publisher","DOI":"10.1016\/j.pestbp.2024.105786","article-title":"Analysis of resistance risk and mechanism\nof the 14\u03b1-demethylation inhibitor ipconazole in Fusarium pseudograminearum","volume":"199","author":"Li","year":"2024","journal-title":"Pestic. 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