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In this study, crude extract from Crassostrea angulata was hydrolyzed with trypsin, Neutrase, and Flavorzyme. The IgE\/IgG-binding activity of the hydrolysates was compared, structures and epitopes were explored, and immune tolerance-inducing potential was evaluated using a T-cell proliferation assay. Under optimized conditions (0.5 h reaction; 1.2% trypsin, 0.8% Neutrase, and 0.4% Flavorzyme), the hydrolysates exhibited markedly reduced immunobinding activity, accompanied by conformational rearrangements and destruction of epitopes. All enzymatic hydrolysates retained certain T-cell epitopes and efficiently stimulated CD4+ T-cell proliferation. Among them, flavorzyme hydrolysate significantly upregulated IL-10 secretion, suggesting its superior in vitro immune tolerance-inducing potential. This study confirms that flavorzyme hydrolysis reduces the allergenicity of oyster, and the hydrolysates possess a strong capacity to induce immune tolerance, supporting the industrial production of hypoallergenic oyster and the development of immune tolerance-inducing agents.<\/jats:p>","DOI":"10.1021\/acs.jafc.6c10218","type":"journal-article","created":{"date-parts":[[2026,9,15]],"date-time":"2026-09-15T16:01:56Z","timestamp":1789488116000},"page":"30385-30394","source":"Crossref","is-referenced-by-count":0,"title":["Preparation of\nHypoallergenic Enzymatic Hydrolysates\nfrom\n                    <i>Crassostrea angulata<\/i>\n                    with\n                    <i>In Vitro<\/i>\n                    Immune Tolerance-Inducing Potential"],"prefix":"10.1021","volume":"74","author":[{"given":"Xin-Yi","family":"Huang","sequence":"first","affiliation":[{"name":"Jimei University , , , ,","place":["Xiamen, Fujian, China, 361021"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Meng","family":"Liu","sequence":"additional","affiliation":[{"name":"Applied Technology Engineering Center of Fujian Provincial Higher Education for Marine Food Nutrition Safety and Advanced Processing , , , ,","place":["Xiamen, Fujian, China, 361100"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ming-Xuan","family":"Wu","sequence":"additional","affiliation":[{"name":"Jimei University , , , ,","place":["Xiamen, Fujian, China, 361021"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Fei","family":"Huan","sequence":"additional","affiliation":[{"name":"Jimei University , , , ,","place":["Xiamen, Fujian, China, 361021"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shuai","family":"Gao","sequence":"additional","affiliation":[{"name":"Jimei University , , , ,","place":["Xiamen, Fujian, China, 361021"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1083-5418","authenticated-orcid":true,"given":"Yang","family":"Yang","sequence":"additional","affiliation":[{"name":"Xiamen Huaxia University , , 288 Tianma Road , , ,","place":["Xiamen, Fujian, China, 361024"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Gui-Xia","family":"Chen","sequence":"additional","affiliation":[{"name":"Women and Children\u2019s Hospital Affiliated to Xiamen University , , ,","place":["Xiamen, Fujian, China, 361003"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Dong","family":"Lai","sequence":"additional","affiliation":[{"name":"The Second Affiliated Hospital of Xiamen Medical College , , ,","place":["Xiamen, Fujian, China, 361021"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4835-7792","authenticated-orcid":true,"given":"Li","family":"Wang","sequence":"additional","affiliation":[{"name":"Jimei University , , , ,","place":["Xiamen, Fujian, China, 361021"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8689-0504","authenticated-orcid":true,"given":"Guang-Ming","family":"Liu","sequence":"additional","affiliation":[{"name":"Jimei University , , , ,","place":["Xiamen, Fujian, China, 361021"]},{"name":"Applied Technology Engineering Center of Fujian Provincial Higher Education for Marine Food Nutrition Safety and Advanced Processing , , , ,","place":["Xiamen, Fujian, China, 361100"]}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"316","published-online":{"date-parts":[[2026,9,15]]},"reference":[{"issue":"4","key":"2026093009080737100_cit1","doi-asserted-by":"publisher","DOI":"10.1111\/raq.70074","article-title":"Nutrition and nutraceutical values\nof commercially important oysters:\nA systematic review","volume":"17","author":"Zhang","year":"2025","journal-title":"Rev. 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