{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,9]],"date-time":"2026-09-09T22:07:26Z","timestamp":1788991646559,"version":"build-2803163510"},"reference-count":55,"publisher":"American Chemical Society (ACS)","issue":"35","license":[{"start":{"date-parts":[[2026,8,29]],"date-time":"2026-08-29T00:00:00Z","timestamp":1787961600000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-029"},{"start":{"date-parts":[[2026,8,29]],"date-time":"2026-08-29T00:00:00Z","timestamp":1787961600000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-037"},{"start":{"date-parts":[[2026,8,29]],"date-time":"2026-08-29T00:00:00Z","timestamp":1787961600000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-045"}],"funder":[{"DOI":"10.13039\/501100002367","name":"Chinese Academy of Sciences","doi-asserted-by":"publisher","award":["176GJHZ2022031GC"],"award-info":[{"award-number":["176GJHZ2022031GC"]}],"id":[{"id":"10.13039\/501100002367","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100002367","name":"Chinese Academy of Sciences","doi-asserted-by":"publisher","award":["XDC0110305"],"award-info":[{"award-number":["XDC0110305"]}],"id":[{"id":"10.13039\/501100002367","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/na","name":"National Basic Research Program of China","doi-asserted-by":"publisher","award":["2023YFF1104604"],"award-info":[{"award-number":["2023YFF1104604"]}],"id":[{"id":"10.13039\/na","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/na","name":"Shanghai Agriculture Applied Technology Development Program","doi-asserted-by":"publisher","award":["T20252010"],"award-info":[{"award-number":["T20252010"]}],"id":[{"id":"10.13039\/na","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2026,9,9]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>Deoxynivalenol (DON), a prevalent mycotoxin produced by Fusarium spp., represents a persistent threat to global food and feed safety. Two innovative strategies were developed herein for efficient DON detoxification. Coupling the genes encoding the aldo-keto reductase AKR13B2 and the pyrroloquinoline quinone-dependent DON dehydrogenase DepA using a flexible linker enabled rational engineering of the bifunctional fusion enzyme BGA (AKR13B2-[GGGGS]2-DepA). This design facilitated proximity-enhanced sequential conversion of DON to 3-keto-DON and the markedly less toxic 3-epi-DON. BGA exhibited significantly enhanced catalytic efficiency, thermal stability, and operational robustness. In parallel, food-grade Kluyveromyces marxianus was engineered for coexpression of codon-optimized DepA and AKR13B2 (termed as KmDepA and KmAKR13B2, respectively), yielding near-complete DON degradation in contaminated wheat grains. KmDepA displayed substantially improved solubility and specific activity relative to its Escherichia coli-expressed counterpart. Collectively, this integrated strategy provides a robust, scalable, and safe method for enzymatic DON remediation, with strong potential for industrial and agricultural applications.<\/jats:p>","DOI":"10.1021\/acs.jafc.6c10441","type":"journal-article","created":{"date-parts":[[2026,9,2]],"date-time":"2026-09-02T12:20:41Z","timestamp":1788351641000},"page":"27954-27966","source":"Crossref","is-referenced-by-count":0,"title":["Bifunctional Enzymes\nfor Deoxynivalenol Degradation\nin Prokaryotic and Eukaryotic Chassis"],"prefix":"10.1021","volume":"74","author":[{"given":"Meichen","family":"Liu","sequence":"first","affiliation":[{"name":"Chinese Academy of Sciences , , ,","place":["Shanghai, People\u2019s Republic of China, 200031"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mingzhu","family":"Guo","sequence":"additional","affiliation":[{"name":"Chinese Academy of Sciences , , ,","place":["Shanghai, People\u2019s Republic of China, 200031"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2280-8644","authenticated-orcid":true,"given":"Ye","family":"Tian","sequence":"additional","affiliation":[{"name":"Chinese Academy of Sciences , , ,","place":["Shanghai, People\u2019s Republic of China, 200031"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Huikang","family":"Lin","sequence":"additional","affiliation":[{"name":"Chinese Academy of Sciences , , ,","place":["Shanghai, People\u2019s Republic of China, 200031"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zheng","family":"Yan","sequence":"additional","affiliation":[{"name":"Chinese Academy of Sciences , , ,","place":["Shanghai, People\u2019s Republic of China, 200031"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Bingxuan","family":"Jia","sequence":"additional","affiliation":[{"name":"Chinese Academy of Sciences , , ,","place":["Shanghai, People\u2019s Republic of China, 200031"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiyan","family":"Sun","sequence":"additional","affiliation":[{"name":"Chinese Academy of Sciences , , ,","place":["Shanghai, People\u2019s Republic of China, 200031"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2160-7253","authenticated-orcid":true,"given":"Sarah","family":"De Saeger","sequence":"additional","affiliation":[{"name":"Ghent University , , Ottergemsesteenweg 460 , ,","place":["Ghent, Belgium, 9000"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Orphe\u0301lie","family":"Lootens","sequence":"additional","affiliation":[{"name":"Ghent University , , Ottergemsesteenweg 460 , ,","place":["Ghent, Belgium, 9000"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Esther","family":"De Rycke","sequence":"additional","affiliation":[{"name":"Ghent University , , Ottergemsesteenweg 460 , ,","place":["Ghent, Belgium, 9000"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jose\u0301","family":"Diana Di Mavungu","sequence":"additional","affiliation":[{"name":"Ghent University , , Ottergemsesteenweg 460 , ,","place":["Ghent, Belgium, 9000"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Dianzhen","family":"Yu","sequence":"additional","affiliation":[{"name":"Chinese Academy of Sciences , , ,","place":["Shanghai, People\u2019s Republic of China, 200031"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7161-1592","authenticated-orcid":true,"given":"Aibo","family":"Wu","sequence":"additional","affiliation":[{"name":"Chinese Academy of Sciences , , ,","place":["Shanghai, People\u2019s Republic of China, 200031"]}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"316","published-online":{"date-parts":[[2026,8,29]]},"reference":[{"key":"2026090917074931600_cit1","doi-asserted-by":"publisher","first-page":"421","DOI":"10.1016\/j.ijbiomac.2022.09.121","article-title":"Structure-based rational design of\na short-chain dehydrogenase\/reductase for improving activity toward\nmycotoxin patulin","volume":"222","author":"Dai","year":"2022","journal-title":"Int. J. Biol. Macromol."},{"key":"2026090917074931600_cit2","doi-asserted-by":"publisher","first-page":"13","DOI":"10.1016\/j.tifs.2017.10.012","article-title":"Deoxynivalenol\nand its masked forms: Characteristics, incidence, control and fate\nduring wheat and wheat based products processing - A review","volume":"71","author":"Khaneghah","year":"2018","journal-title":"Trends Food Sci. Technol."},{"issue":"8","key":"2026090917074931600_cit3","doi-asserted-by":"publisher","first-page":"1346","DOI":"10.1080\/10408398.2019.1571479","article-title":"Global occurrence\nof deoxynivalenol in food commodities and exposure risk assessment\nin humans in the last decade: a survey","volume":"60","author":"Mishra","year":"2020","journal-title":"Crit.\nRev. Food Sci. Nutr."},{"key":"2026090917074931600_cit4","doi-asserted-by":"publisher","first-page":"800","DOI":"10.1016\/j.ijbiomac.2021.07.085","article-title":"Genome mining reveals\nthe genes of\ncarboxypeptidase for OTA-detoxification in Bacillus subtilis CW14","volume":"186","author":"Xu","year":"2021","journal-title":"Int. J. Biol. Macromol."},{"key":"2026090917074931600_cit5","doi-asserted-by":"publisher","first-page":"233","DOI":"10.1016\/j.tifs.2019.12.021","article-title":"Recent\nadvances on toxicity and determination methods of mycotoxins in foodstuffs","volume":"96","author":"Yang","year":"2020","journal-title":"Trends Food Sci. Technol."},{"issue":"1","key":"2026090917074931600_cit6","doi-asserted-by":"publisher","first-page":"13","DOI":"10.1038\/s41467-024-45502-w","article-title":"Regulation of TRI5 expression and deoxynivalenol\nbiosynthesis by a long non-coding RNA in Fusarium graminearum","volume":"15","author":"Huang","year":"2024","journal-title":"Nat. Commun."},{"issue":"4","key":"2026090917074931600_cit7","doi-asserted-by":"publisher","first-page":"17","DOI":"10.1007\/s13205-020-2158-x","article-title":"Fusarium head blight\nin wheat: contemporary status and molecular approaches","volume":"10","author":"Khan","year":"2020","journal-title":"3 Biotech"},{"issue":"6","key":"2026090917074931600_cit8","doi-asserted-by":"publisher","first-page":"515","DOI":"10.1111\/j.1364-3703.2004.00252.x","article-title":"Heading for disaster: Fusarium graminearum on cereal crops","volume":"5","author":"Goswami","year":"2004","journal-title":"Mol. Plant Pathol."},{"key":"2026090917074931600_cit9","doi-asserted-by":"publisher","first-page":"15","DOI":"10.1146\/annurev-phyto-082718-100318","article-title":"Fusarium graminearum Trichothecene Mycotoxins: Biosynthesis, Regulation,\nand Management","volume-title":"Annual Review of Phytopathology","author":"Chen","year":"2019"},{"key":"2026090917074931600_cit10","doi-asserted-by":"publisher","first-page":"307","DOI":"10.1016\/j.tifs.2021.06.045","article-title":"Maize food\nchain and mycotoxins: A review on occurrence studies","volume":"115","author":"Leite","year":"2021","journal-title":"Trends Food Sci. Technol."},{"issue":"3","key":"2026090917074931600_cit11","doi-asserted-by":"publisher","first-page":"200","DOI":"10.3390\/toxins12030200","article-title":"Natural Occurrence of Deoxynivalenol\nand Its Acetylated Derivatives in Chinese Maize and Wheat Collected\nin 2017","volume":"12","author":"Yan","year":"2020","journal-title":"Toxins"},{"issue":"2","key":"2026090917074931600_cit12","doi-asserted-by":"publisher","first-page":"895","DOI":"10.1111\/1541-4337.12545","article-title":"Deoxynivalenol: Masked forms, fate\nduring food processing, and potential biological remedies","volume":"19","author":"Guo","year":"2020","journal-title":"Compr. Rev. Food Sci. Food Saf."},{"key":"2026090917074931600_cit13","doi-asserted-by":"publisher","first-page":"96","DOI":"10.1016\/j.tifs.2022.04.002","article-title":"Elimination of Fusarium mycotoxin deoxynivalenol (DON) via microbial and\nenzymatic strategies:\nCurrent status and future perspectives","volume":"124","author":"Tian","year":"2022","journal-title":"Trends\nFood Sci. Technol."},{"key":"2026090917074931600_cit14","doi-asserted-by":"publisher","first-page":"110799","DOI":"10.1016\/j.cbi.2023.110799","article-title":"Recalling\nthe reported toxicity assessment\nof deoxynivalenol, mitigating strategies and its toxicity mechanisms:\nComprehensive review","volume":"387","author":"Murtaza","year":"2024","journal-title":"Chem.-Biol. Interact."},{"key":"2026090917074931600_cit15","doi-asserted-by":"publisher","first-page":"111357","DOI":"10.1016\/j.fct.2020.111357","article-title":"Effects of deoxynivalenol on mitochondrial\ndynamics\nand autophagy in pig spleen lymphocytes","volume":"140","author":"Ren","year":"2020","journal-title":"Food\nChem. Toxicol."},{"issue":"18","key":"2026090917074931600_cit16","doi-asserted-by":"publisher","first-page":"10616","DOI":"10.1021\/acs.jafc.4c01151","article-title":"Deoxynivalenol Inhibits\nProgenitor Leydig Cell Development by Stimulating Mitochondrial Fission\nin Rats","volume":"72","author":"Yang","year":"2024","journal-title":"J. Agric. Food Chem."},{"issue":"4","key":"2026090917074931600_cit17","doi-asserted-by":"publisher","first-page":"1777","DOI":"10.1111\/1541-4337.12594","article-title":"Application\nof ozone for degradation\nof mycotoxins in food: A review","volume":"19","author":"Afsah-Hejri","year":"2020","journal-title":"Compr. Rev.\nFood Sci. Food Saf."},{"issue":"21","key":"2026090917074931600_cit18","doi-asserted-by":"publisher","first-page":"5903","DOI":"10.1080\/10408398.2021.1895056","article-title":"Mechanisms of deoxynivalenol (DON) degradation during\ndifferent treatments: a review","volume":"62","author":"Feizollahi","year":"2022","journal-title":"Crit. Rev. Food\nSci. Nutr."},{"key":"2026090917074931600_cit19","doi-asserted-by":"publisher","first-page":"22","DOI":"10.3389\/fmicb.2024.1434987","article-title":"Bioenzymatic detoxification of mycotoxins","volume":"15","author":"Liu","year":"2024","journal-title":"Front. Microbiol."},{"issue":"3","key":"2026090917074931600_cit20","doi-asserted-by":"publisher","first-page":"491","DOI":"10.1007\/s00253-011-3401-5","article-title":"Biological\ndetoxification of the mycotoxin deoxynivalenol and its use in genetically\nengineered crops and feed additives","volume":"91","author":"Karlovsky","year":"2011","journal-title":"Appl. Microbiol.\nBiotechnol."},{"issue":"5","key":"2026090917074931600_cit21","doi-asserted-by":"publisher","first-page":"1619","DOI":"10.1128\/AEM.03227-12","article-title":"Bacterial\nCytochrome P450 System\nCatabolizing the Fusarium Toxin Deoxynivalenol","volume":"79","author":"Ito","year":"2013","journal-title":"Appl. Environ. Microbiol."},{"issue":"6","key":"2026090917074931600_cit22","doi-asserted-by":"publisher","first-page":"1106","DOI":"10.1111\/1751-7915.12874","article-title":"The enzymatic detoxification\nof the mycotoxin deoxynivalenol: identification of DepA from the DON\nepimerization pathway","volume":"11","author":"Carere","year":"2018","journal-title":"Microb. Biotechnol."},{"key":"2026090917074931600_cit23","doi-asserted-by":"publisher","first-page":"13","DOI":"10.1038\/s41598-017-08799-w","article-title":"An aldo-keto reductase is responsible\nfor Fusarium toxin-degrading activity in a soil Sphingomonas strain","volume":"7","author":"He","year":"2017","journal-title":"Sci. Rep"},{"key":"2026090917074931600_cit24","doi-asserted-by":"publisher","first-page":"126703","DOI":"10.1016\/j.foodchem.2020.126703","article-title":"A quinone-dependent dehydrogenase\nand two NADPH-dependent\naldo\/keto reductases detoxify deoxynivalenol in wheat via epimerization\nin a Devosia strain","volume":"321","author":"He","year":"2020","journal-title":"Food Chem."},{"key":"2026090917074931600_cit25","doi-asserted-by":"publisher","first-page":"108834","DOI":"10.1016\/j.foodcont.2022.108834","article-title":"A quinoprotein\ndehydrogenase from Pelagibacterium halotolerans ANSP101\noxidizes deoxynivalenol to 3-keto-deoxynivalenol","volume":"136","author":"Qin","year":"2022","journal-title":"Food Control"},{"key":"2026090917074931600_cit26","doi-asserted-by":"publisher","first-page":"250","DOI":"10.1016\/j.fct.2015.09.003","article-title":"Toxicology of 3-epi-deoxynivalenol,\na deoxynivalenol-transformation product by Devosia mutans 17\u20132-E-8","volume":"84","author":"He","year":"2015","journal-title":"Food Chem. Toxicol."},{"key":"2026090917074931600_cit27","doi-asserted-by":"publisher","first-page":"15","DOI":"10.3389\/fmicb.2016.00572","article-title":"Bacterial Epimerization\nas a Route for Deoxynivalenol Detoxification:\nthe Influence of Growth and Environmental Conditions","volume":"7","author":"He","year":"2016","journal-title":"Front. Microbiol."},{"key":"2026090917074931600_cit28","doi-asserted-by":"publisher","first-page":"13","DOI":"10.1038\/srep29105","article-title":"Microbial biotransformation of DON:\nmolecular basis for reduced toxicity","volume":"6","author":"Pierron","year":"2016","journal-title":"Sci. Rep"},{"issue":"10","key":"2026090917074931600_cit29","doi-asserted-by":"publisher","first-page":"3825","DOI":"10.1128\/aem.63.10.3825-3830.1997","article-title":"Novel detoxification of the trichothecene\nmycotoxin deoxynivalenol by a soil bacterium isolated by enrichment\nculture","volume":"63","author":"Shima","year":"1997","journal-title":"Appl. Environ. Microbiol."},{"issue":"4","key":"2026090917074931600_cit30","doi-asserted-by":"publisher","first-page":"299","DOI":"10.1038\/nchem.2459","article-title":"Substrate channelling\nas an approach to cascade reactions","volume":"8","author":"Wheeldon","year":"2016","journal-title":"Nat. Chem."},{"issue":"22","key":"2026090917074931600_cit31","article-title":"GDHS-MGH Evolved Peroxygenase\u2013Aryl Alcohol\nOxidase Fusions\nfor Self-Sufficient Oxyfunctionalization Reactions","volume":"10","author":"Alcalde","year":"2020","journal-title":"ACS Catal."},{"issue":"3","key":"2026090917074931600_cit32","doi-asserted-by":"publisher","first-page":"297","DOI":"10.1016\/j.biotechadv.2009.01.008","article-title":"Production\nof recombinant proteins by microbes and\nhigher organisms","volume":"27","author":"Demain","year":"2009","journal-title":"Biotechnol. Adv."},{"key":"2026090917074931600_cit33","doi-asserted-by":"publisher","first-page":"8","DOI":"10.1186\/1475-2859-8-17","article-title":"Microbial\nfactories for recombinant pharmaceuticals","volume":"8","author":"Ferrer-Miralles","year":"2009","journal-title":"Microb.\nCell Fact."},{"issue":"3","key":"2026090917074931600_cit34","doi-asserted-by":"publisher","first-page":"261","DOI":"10.1007\/bf00873690","article-title":"Physiological and technological aspects of large-scale\nheterologous-protein production with yeasts","volume":"67","author":"Hensing","year":"1995","journal-title":"Antonie van Leeuwenhoek"},{"issue":"11","key":"2026090917074931600_cit35","doi-asserted-by":"publisher","first-page":"1621","DOI":"10.1002\/cbic.202000023","article-title":"Whole-Cell Biocatalysis in Seawater: New Halotolerant\nYeast Strains for the Regio- and Stereoselectivity Reduction of 1-Phenylpropane-1,2-Dione\nin Saline-Rich Media","volume":"21","author":"Andreu","year":"2020","journal-title":"ChemBioChem"},{"issue":"24","key":"2026090917074931600_cit36","doi-asserted-by":"publisher","first-page":"7514","DOI":"10.1128\/AEM.01854-08","article-title":"High-Temperature Ethanol\nFermentation and Transformation with Linear DNA in the Thermotolerant\nYeast Kluyveromyces marxianus DMKU3\u20131042","volume":"74","author":"Nonklang","year":"2008","journal-title":"Appl. Environ. Microbiol."},{"issue":"6","key":"2026090917074931600_cit37","doi-asserted-by":"publisher","first-page":"2076","DOI":"10.1111\/j.1365-2672.2008.03903.x","article-title":"Ethanol fermentation\nwith Kluyveromyces marxianus from Jerusalem artichoke\ngrown in salina and irrigated with a mixture of seawater and freshwater","volume":"105","author":"Yuan","year":"2008","journal-title":"J. Appl. Microbiol."},{"issue":"1\u20132","key":"2026090917074931600_cit38","doi-asserted-by":"publisher","first-page":"17","DOI":"10.1016\/j.fbr.2010.01.001","article-title":"Kluyveromyces\nmarxianus: A yeast emerging from its\nsister\u2019s shadow","volume":"24","author":"Lane","year":"2010","journal-title":"Fungal Biol. Rev."},{"issue":"4","key":"2026090917074931600_cit39","doi-asserted-by":"publisher","first-page":"1573","DOI":"10.1007\/s00253-011-3218-2","article-title":"Growth and\nethanol fermentation ability on hexose and\npentose sugars and glucose effect under various conditions in thermotolerant\nyeast Kluyveromyces marxianus","volume":"90","author":"Rodrussamee","year":"2011","journal-title":"Appl. Microbiol. Biotechnol."},{"issue":"10","key":"2026090917074931600_cit40","doi-asserted-by":"publisher","first-page":"1274","DOI":"10.1002\/biot.201600060","article-title":"High throughput,\ncolorimetric\nscreening of microbial ester biosynthesis reveals high ethyl acetate\nproduction from Kluyveromyces marxianus on C5, C6,\nand C12 carbon sources","volume":"11","author":"Lo\u0308bs","year":"2016","journal-title":"Biotechnol. J."},{"key":"2026090917074931600_cit41","doi-asserted-by":"publisher","first-page":"14","DOI":"10.1186\/s13068-017-0854-5","article-title":"CRISPR-Cas9-enabled\ngenetic disruptions for understanding ethanol and ethyl acetate biosynthesis\nin Kluyveromyces marxianus","volume":"10","author":"Lo\u0308bs","year":"2017","journal-title":"Biotechnol. Biofuels"},{"key":"2026090917074931600_cit42","doi-asserted-by":"publisher","first-page":"12","DOI":"10.1186\/1475-2859-9-4","article-title":"Heterologous\nexpression of glucose oxidase in the yeast Kluyveromyces marxianus","volume":"9","author":"Rocha","year":"2010","journal-title":"Microb. Cell\nFact."},{"issue":"1","key":"2026090917074931600_cit43","doi-asserted-by":"publisher","first-page":"15","DOI":"10.1186\/s13068-020-01852-3","article-title":"CRISPR-mediated multigene\nintegration enables Shikimate pathway refactoring for enhanced 2-phenylethanol\nbiosynthesis in Kluyveromyces marxianus","volume":"14","author":"Li","year":"2021","journal-title":"Biotechnol. Biofuels"},{"issue":"1","key":"2026090917074931600_cit44","doi-asserted-by":"publisher","first-page":"15","DOI":"10.1038\/s41467-024-47519-7","article-title":"Synthetic intrinsically\ndisordered protein fusion tags that enhance protein solubility","volume":"15","author":"Tang","year":"2024","journal-title":"Nat. Commun."},{"key":"2026090917074931600_cit45","doi-asserted-by":"publisher","first-page":"7","DOI":"10.1038\/s41598-018-37034-3","article-title":"Can enzyme\nproximity accelerate cascade reactions?","volume":"9","author":"Kuzmak","year":"2019","journal-title":"Sci.\nRep"},{"key":"2026090917074931600_cit46","doi-asserted-by":"publisher","first-page":"12","DOI":"10.1038\/s41467-018-04543-8","article-title":"The role of dynamic enzyme assemblies\nand substrate\nchannelling in metabolic regulation","volume":"9","author":"Sweetlove","year":"2018","journal-title":"Nat. Commun."},{"issue":"22","key":"2026090917074931600_cit47","doi-asserted-by":"publisher","first-page":"6764","DOI":"10.1021\/acs.jafc.2c01083","article-title":"Structure-Function\nAnalysis of a Quinone-Dependent Dehydrogenase Capable of Deoxynivalenol\nDetoxification","volume":"70","author":"Yang","year":"2022","journal-title":"J. Agric. Food Chem."},{"issue":"1","key":"2026090917074931600_cit48","doi-asserted-by":"publisher","first-page":"13","DOI":"10.1038\/s41598-022-19040-8","article-title":"Structure-function\ncharacterization of an aldo-keto reductase involved in detoxification\nof the mycotoxin, deoxynivalenol","volume":"12","author":"Abraham","year":"2022","journal-title":"Sci. Rep"},{"issue":"11","key":"2026090917074931600_cit49","doi-asserted-by":"publisher","first-page":"2193","DOI":"10.1007\/s10529-011-0676-7","article-title":"Construction and\ncharacterization of a fusion \u03b2-1,3\u20131,4-glucanase\nto improve hydrolytic activity and thermostability","volume":"33","author":"Sun","year":"2011","journal-title":"Biotechnol. Lett."},{"issue":"2","key":"2026090917074931600_cit50","doi-asserted-by":"publisher","first-page":"91","DOI":"10.1023\/A:1014419323550","article-title":"Deoxynivalenol-nonproducing Fusarium graminearum Causes Initial Infection, but does\nnot Cause Disease Spread in Wheat Spikes","volume":"153","author":"Bai","year":"2002","journal-title":"Mycopathologia"},{"key":"2026090917074931600_cit51","doi-asserted-by":"publisher","first-page":"134695","DOI":"10.1016\/j.jhazmat.2024.134695","article-title":"Co-contamination\nand interactions\nof multiple mycotoxins and heavy metals in rice, maize, soybeans,\nand wheat flour marketed in Shanghai City","volume":"474","author":"Zhu","year":"2024","journal-title":"J.\nHazard. Mater."},{"issue":"4","key":"2026090917074931600_cit52","doi-asserted-by":"publisher","first-page":"1308","DOI":"10.3390\/toxins6041308","article-title":"Systemic Growth of F. graminearum in\nWheat Plants and Related Accumulation of Deoxynivalenol","volume":"6","author":"Moretti","year":"2014","journal-title":"Toxins"},{"key":"2026090917074931600_cit53","doi-asserted-by":"publisher","first-page":"133743","DOI":"10.1016\/j.biortech.2025.133743","article-title":"Unlocking Kluyveromyces marxianus for efficient\nprotein expression and single-cell protein production","volume":"442","author":"Cui","year":"2026","journal-title":"Bioresour. Technol."},{"issue":"3","key":"2026090917074931600_cit54","doi-asserted-by":"publisher","first-page":"764","DOI":"10.1016\/j.cell.2024.11.013","article-title":"Structural\ndeterminants of co-translational protein complex assembly","volume":"188","author":"Mallik","year":"2025","journal-title":"Cell"},{"key":"2026090917074931600_cit55","doi-asserted-by":"publisher","first-page":"129512","DOI":"10.1016\/j.ijbiomac.2024.129512","article-title":"Self-cascade\ndeoxynivalenol\ndetoxification by an artificial enzyme with bifunctions of dehydrogenase\nand aldo\/keto reductase from genome mining","volume":"261","author":"Niu","year":"2024","journal-title":"Int. J. Biol. Macromol."}],"container-title":["Journal of Agricultural\nand Food Chemistry"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/pubs.acs.org\/doi\/pdf\/10.1021\/acs.jafc.6c10441","content-type":"application\/pdf","content-version":"vor","intended-application":"unspecified"},{"URL":"https:\/\/pubs.acs.org\/jafcau\/article-pdf\/74\/35\/27954\/67739512\/acs.jafc.6c10441.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"syndication"},{"URL":"https:\/\/pubs.acs.org\/jafcau\/article-pdf\/74\/35\/27954\/67739512\/acs.jafc.6c10441.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,9,9]],"date-time":"2026-09-09T21:16:26Z","timestamp":1788988586000},"score":1,"resource":{"primary":{"URL":"https:\/\/pubs.acs.org\/jafcau\/article\/74\/35\/27954\/5361410\/Bifunctional-Enzymes-for-Deoxynivalenol"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,8,29]]},"references-count":55,"journal-issue":{"issue":"35","published-online":{"date-parts":[[2026,8,29]]},"published-print":{"date-parts":[[2026,9,9]]}},"URL":"https:\/\/doi.org\/10.1021\/acs.jafc.6c10441","relation":{},"ISSN":["0021-8561","1520-5118"],"issn-type":[{"value":"0021-8561","type":"print"},{"value":"1520-5118","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026,8,29]]}}}